Sublinear response in lacZ mutant frequency of Muta™Mouse spermatogonial stem cells after low dose subchronic exposure to N-ethyl-N-nitrosourea

Environmental and Molecular Mutagenesis

Volumn 56, Issue 4, 2015, Pages 347-355

  O'Brien, Jason M ^a   Walker, Mike ^a   Sivathayalan, Ahalya ^a   Douglas, George R ^a   Yauk, Carole L ^a   Marchetti, Francesco ^a  

^a HEALTH CANADA   (Canada)

Author keywords

Dose response modeling; Germ cell mutation; N ethyl N nitrosourea; Spermatogonia; Threshold; Transgenic rodent mutation assay

Indexed keywords

BETA GALACTOSIDASE; ETHYLNITROSOUREA; GENOMIC DNA; MUTAGENIC AGENT;

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DNA ISOLATION; DNA REPAIR; DOSE RESPONSE; EPIDIDYMIS TAIL; EXPOSURE; GENE FREQUENCY; GENE MUTATION; GENOTOXICITY; MALE; MOUSE; MUTANT FREQUENCY; NONHUMAN; REPRODUCTIVE TOXICITY; RISK ASSESSMENT; SPERMATOGONIUM; STEM CELL; TRANSGENIC MOUSE; ADULT STEM CELL; ANIMAL; DNA DAMAGE; DRUG EFFECTS; LACTOSE OPERON; MUTATION; MUTATION RATE; PROCEDURES; TOXICITY TESTING;

RODENTIA;

ADULT STEM CELLS; ANIMALS; DNA DAMAGE; DOSE-RESPONSE RELATIONSHIP, DRUG; ETHYLNITROSOUREA; LAC OPERON; MALE; MICE, TRANSGENIC; MUTAGENS; MUTATION; MUTATION RATE; TOXICITY TESTS, SUBCHRONIC;

EID: 84928066320     PISSN: 08936692     EISSN: 10982280     Source Type: Journal    
DOI: 10.1002/em.21932     Document Type: Article

References (40)

1. Akaike H. 1974. A new look at the statistical model identification. IEEE Trans Autom Control 19:716-723.
2. Bolker B. 2013. bbmle: Tools for general maximum likelihood estimation. http://cran.r-project.org/web/packages/bbmle/bbmle.pdf (accessed July 3, 2014).
3. Bronstein SM, Cochrane JE, Craft TR, Swenberg JA, Skopek TR. 1991. Toxicity, mutagenicity, and mutational spectra of N-ethyl-N-nitrosourea in human cell lines with different DNA repair phenotypes. Cancer Res 51:5188-5197.
4. Bronstein SM, Skopek TR, Swenberg JA. 1992. Efficient repair of O6-ethylguanine, but not O4-ethylthymine or O2-ethylthymine, is dependent upon O6-alkylguanine-DNA alkyltransferase and nucleotide excision repair activities in human cells. Cancer Res 52:2008-2011.
5. Bryce SM, Avlasevich SL, Bemis JC, Phonethepswath S, Dertinger SD. 2010. Miniaturized flow cytometric in vitro micronucleus assay represents an efficient tool for comprehensively characterizing genotoxicity dose-response relationships. Mutat Res 703:191-199.
6. Cao X, Mittelstaedt RA, Pearce MG, Allen BC, Soeteman-Hernández LG, Johnson GE, Bigger CA, Heflich RH. 2014. Quantitative dose-response analysis of ethyl methanesulfonate genotoxicity in adult gpt-delta transgenic mice. Environ Mol Mutagen 55:385-399.
7. Conrad DF, Keebler JE, DePristo MA, Lindsay SJ, Zhang Y, Casals F, Idaghdour Y, Hartl CL, Torroja C, Garimella KV, et al. 2011. Variation in genome-wide mutation rates within and between human families. Nat Genet 43:712-714.
8. Cosentino L, Heddle JA. 1999. Effects of extended chronic exposures on endogenous and transgenic loci: Implications for low-dose extrapolations. Environ Mol Mutagen 34:208-215.
9. Demarini DM. 2012. Declaring the existence of human germ-cell mutagens. Environ Mol Mutagen 53:166-172.
10. Doak SH, Jenkins GJ, Johnson GE, Quick E, Parry EM, Parry JM. 2007. Mechanistic influences for mutation induction curves after exposure to DNA-reactive carcinogens. Cancer Res 67:3904-3911.
11. Douglas GR, Jiao J, Gingerich JD, Gossen JA, Soper LM. 1995. Temporal and molecular characteristics of mutations induced by ethylnitrosourea in germ cells isolated from seminiferous tubules and in spermatozoa of lacZ transgenic mice. Proc Natl Acad Sci USA 92:7485-7489.
12. Favor J, Sund M, Neuhauser-Klaus A, Ehling UH. 1990. A dose-response analysis of ethylnitrosourea-induced recessive specific-locus mutations in treated spermatogonia of the mouse. Mutat Res 231:47-54.
13. Gocke E, Muller L. 2009. In vivo studies in the mouse to define a threshold for the genotoxicity of EMS and ENU. Mutat Res 678:101-107.
14. Gollapudi BB, Johnson GE, Hernandez LG, Pottenger LH, Dearfield KL, Jeffrey AM, Julien E, Kim JH, Lovell DP, Macgregor JT, et al. 2013. Quantitative approaches for assessing dose-response relationships in genetic toxicology studies. Environ Mol Mutagen 54:8-18.
15. Johnson GE, Doak SH, Griffiths SM, Quick EL, Skibinski DO, Zair ZM, Jenkins GJ. 2009. Non-linear dose-response of DNA-reactive genotoxins: Recommendations for data analysis. Mutat Res 678:95-100.
16. Johnson GE, Zair Z, Bodger OG, Lewis PD, Rees BJ, Verma JR, Thomas AD, Doak SH, Jenkins GJS. 2012. Investigating mechanisms for non-linear genotoxic responses, and analysing their effects in binary combination. Genes Environ 34:179-185.
17. Johnson GE, Soeteman-Hernandez LG, Gollapudi BB, Bodger OG, Dearfield KL, Heflich RH, Hixon JG, Lovell DP, Macgregor JT, Pottenger LH, et al. 2014. Derivation of point of departure (PoD) estimates in genetic toxicology studies and their potential applications in risk assessment. Environ Mol Mutagen 55:609-623.
18. Julien E, Boobis AR, Olin SS, Ilsi Research Foundation Threshold Working Group. 2009. The key events dose-response framework: A cross-disciplinary mode-of-action based approach to examining dose-response and thresholds. Crit Rev Food Sci Nutr 49:682-689.
19. Justice MJ, Carpenter DA, Favor J, Neuhauser-Klaus A, Hrabe de Angelis M, Soewarto D, Moser A, Cordes S, Miller D, et al. 2000. Effects of ENU dosage on mouse strains. Mamm Genome 11:484-488.
20. Kong A, Frigge ML, Masson G, Besenbacher S, Sulem P, Magnusson G, Gudjonsson SA, Sigurdsson A, Jonasdottir A, Jonasdottir A, et al. 2012. Rate of de novo mutations and the importance of father's age to disease risk. Nature 488:471-475.
21. Lutz WK. 1998. Dose-response relationships in chemical carcinogenesis: Superposition of different mechanisms of action, resulting in linear-nonlinear curves, practical thresholds, J-shapes. Mutat Res 405:117-124.
22. Lynch AM, Giddings A, Custer L, Gleason C, Henwood A, Aylott M, Kenny J. 2011. International Pig-A gene mutation assay trial (stage III): Results with N-methyl-N-nitrosourea. Environ Mol Mutagen 52:699-710.
23. Muller L, Gocke E, Lave T, Pfister T. 2009. Ethyl methanesulfonate toxicity in Viracept-A comprehensive human risk assessment based on threshold data for genotoxicity. Toxicol Lett 190:317-329.
24. Murphey P, McLean DJ, McMahan CA, Walter CA, McCarrey JR. 2013. Enhanced genetic integrity in mouse germ cells. Biol Reprod 88:6.
25. Noveroske JK, Weber JS, Justice MJ. 2000. The mutagenic action of N-ethyl-N-nitrosourea in the mouse. Mamm Genome 11:478-483.
26. O'Brien JM, Beal MA, Gingerich JD, Soper L, Douglas GR, Yauk CL, Marchetti F. 2014. Transgenic rodent assay for quantifying male germ cell mutation frequency. J Vis Exp 90:e51576.
27. OECD. 2009. Detailed review paper on transgenic rodent assays. Organization for Economic Co-operation and Development. http://www.oecd.org/officialdocuments/publicdisplaydocumentpdf/?cote=ENV/JM/MONO%282009%297&docLanguage=En (accessed July 3, 2014).
28. OECD. 2013. OECD guideline for the testing of chemicals: Transgenic rodent somatic and germ cell gene mutation assays. doi:10.1787/9789264203907-en.
29. Pegg AE. 2011. Multifaceted roles of alkyltransferase and related proteins in DNA repair, DNA damage, resistance to chemotherapy, and research tools. Chem Res Toxicol 24:618-639.
30. Pottenger LH, Gollapudi BB. 2010. Genotoxicity testing: Moving beyond qualitative "screen and bin" approach towards characterization of dose-response and thresholds. Environ Mol Mutagen 51:792-799.
31. Pottenger LH, Schisler MR, Zhang F, Bartels MJ, Fontaine DD, McFadden LG, Bhaskar Gollapudi B. 2009. Dose-response and operational thresholds/NOAELs for in vitro mutagenic effects from DNA-reactive mutagens, MMS and MNU. Mutat Res 678:138-147.
32. Russell WL, Hunsicker PR, Raymer GD, Steele MH, Stelzner KF, Thompson HM. 1982. Dose-response curve for ethylnitrosourea-induced specific-locus mutations in mouse spermatogonia. Proc Natl Acad Sci USA 79:3589-3591.
33. SAS Institute Inc. 2008. SAS/STAT 9.2 User's Guide. Cary, NC, USA: SAS Institute Inc.
34. Shwed PS, Crosthwait J, Douglas GR, Seligy VL. 2010. Characterisation of MutaMouse lambdagt10-lacZ transgene: Evidence for in vivo rearrangements. Mutagenesis 25:609-616.
35. Thomas AD, Jenkins GJ, Kaina B, Bodger OG, Tomaszowski KH, Lewis PD, Doak SH, Johnson GE. 2013. Influence of DNA repair on nonlinear dose-responses for mutation. Toxicol Sci 132:87-95.
36. van Delft JH, Baan RA. 1995. Germ cell mutagenesis in lambda lacZ transgenic mice treated with ethylnitrosourea; comparison with specific-locus test. Mutagenesis 10:209-214.
37. van Delft JH, Bergmans A, van Dam FJ, Tates AD, Howard L, Winton DJ, Baan RA. 1998. Gene-mutation assays in lambda lacZ transgenic mice: Comparison of lacZ with endogenous genes in splenocytes and small intestinal epithelium. Mutat Res 415:85-96.
38. van Zeeland AA, de Groot A, Neuhauser-Klaus A. 1990. DNA adduct formation in mouse testis by ethylating agents: A comparison with germ-cell mutagenesis. Mutat Res 231:55-62.
39. Wilhelm D, Bender K, Knebel A, Angel P. 1997. The level of intracellular glutathione is a key regulator for the induction of stress-activated signal transduction pathways including Jun N-terminal protein kinases and p38 kinase by alkylating agents. Mol Cell Biol 17:4792-4800.
40. Zair ZM, Jenkins GJ, Doak SH, Singh R, Brown K, Johnson GE. 2011. N-methylpurine DNA glycosylase plays a pivotal role in the threshold response of ethyl methanesulfonate-induced chromosome damage. Toxicol Sci 119:346-358.