G-protein coupled receptor solubilization and purification for biophysical analysis and functional studies, in the total absence of detergent

Bioscience Reports

Volumn 35, Issue , 2015, Pages

  Jamshad, Mohammed ^a   Charlton, Jack ^a   Lin, Yu Pin ^a   Routledge, Sarah J ^b   Bawa, Zharain ^b   Knowles, Timothy J ^a   Overduin, Michael ^a   Dekker, Niek ^c   Dafforn, Tim R ^a   Bill, Roslyn M ^b   Poyner, David R ^b   Wheatley, Mark ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^b ASTON UNIVERSITY   (United Kingdom)

^c ASTRAZENECA R AND D   (Sweden)

Author keywords

Adenosine receptor; Detergent free; G protein coupled receptor (GPCR); Protein thermostability; Structure

Indexed keywords

ADENOSINE A2A RECEPTOR; NANODISC; SCAFFOLD PROTEIN; STYRENE MALEIC ANHYDRIDE COPOLYMER; DODECYL MALTOPYRANOSIDE; MALTOSE; RECOMBINANT PROTEIN;

ARTICLE; CIRCULAR DICHROISM; CONTROLLED STUDY; EMBRYO; ENCAPSULATION; HUMAN; HUMAN CELL; KOMAGATAELLA PASTORIS; LIGAND BINDING; MAMMAL CELL; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; RECEPTOR SOLUBILIZATION; THERMOSTABILITY; ANALOGS AND DERIVATIVES; BIOSYNTHESIS; CELL LINE; CHEMISTRY; GENETICS; HEK293 CELL LINE; ISOLATION AND PURIFICATION; METABOLISM; PICHIA; PROTEIN STABILITY; SOLUBILITY;

MAMMALIA; PICHIA PASTORIS;

CELL LINE; HEK293 CELLS; HUMANS; MALTOSE; PICHIA; PROTEIN STABILITY; RECEPTOR, ADENOSINE A2A; RECOMBINANT PROTEINS; SOLUBILITY;

EID: 84927919762     PISSN: 01448463     EISSN: 15734935     Source Type: Journal    
DOI: 10.1042/BSR20140171     Document Type: Article

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