-
1
-
-
79955602637
-
High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome
-
Alsford, S. et al. High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome. Genome Res. 21, 915-924 (2011).
-
(2011)
Genome Res.
, vol.21
, pp. 915-924
-
-
Alsford, S.1
-
2
-
-
84856955769
-
High-throughput decoding of antitrypanosomal drug efficacy and resistance
-
Alsford, S. et al. High-throughput decoding of antitrypanosomal drug efficacy and resistance. Nature 482, 232-236 (2012).
-
(2012)
Nature
, vol.482
, pp. 232-236
-
-
Alsford, S.1
-
3
-
-
78951491179
-
Genome-wide RNAi screens in African trypanosomes identify the nifurtimox activator NTR and the eflornithine transporter AAT6
-
Baker, N., Alsford, S., Horn, D. Genome-wide RNAi screens in African trypanosomes identify the nifurtimox activator NTR and the eflornithine transporter AAT6. Mol. Biochem. Parasitol. 176, 55-57 (2011).
-
(2011)
Mol. Biochem. Parasitol.
, vol.176
, pp. 55-57
-
-
Baker, N.1
Alsford, S.2
Horn, D.3
-
4
-
-
78049436362
-
Genome-wide RNAi screens in bloodstream form trypanosomes identify drug transporters
-
Schumann Burkard, G., Jutzi, P., Roditi, I. Genome-wide RNAi screens in bloodstream form trypanosomes identify drug transporters. Mol. Biochem. Parasitol. 175, 91-94 (2011).
-
(2011)
Mol. Biochem. Parasitol.
, vol.175
, pp. 91-94
-
-
Schumann Burkard, G.1
Jutzi, P.2
Roditi, I.3
-
5
-
-
84877805892
-
Nucleolar proteins regulate stage-specific gene expression and ribosomal RNA maturation in Trypanosoma brucei
-
Schumann Burkard, G. et al. Nucleolar proteins regulate stage-specific gene expression and ribosomal RNA maturation in Trypanosoma brucei. Mol. Microbiol. 88, 827-840 (2013).
-
(2013)
Mol. Microbiol.
, vol.88
, pp. 827-840
-
-
Schumann Burkard, G.1
-
6
-
-
84884224511
-
Cyclic AMP effectors in African trypanosomes revealed by genome-scale RNA interference library screening for resistance to the phosphodiesterase inhibitor CpdA
-
Gould, M.K. et al. Cyclic AMP effectors in African trypanosomes revealed by genome-scale RNA interference library screening for resistance to the phosphodiesterase inhibitor CpdA. Antimicrob. Agents Chemother. 57, 4882-4893 (2013).
-
(2013)
Antimicrob. Agents Chemother.
, vol.57
, pp. 4882-4893
-
-
Gould, M.K.1
-
7
-
-
84895900845
-
Genome-wide dissection of the quorum sensing signalling pathway in Trypanosoma brucei
-
Mony, B.M. et al. Genome-wide dissection of the quorum sensing signalling pathway in Trypanosoma brucei. Nature 505, 681-685 (2014).
-
(2014)
Nature
, vol.505
, pp. 681-685
-
-
Mony, B.M.1
-
8
-
-
84901676974
-
Cathepsin-L can resist lysis by human serum in Trypanosoma brucei brucei
-
Alsford, S., Currier, R.B., Guerra-Assuncao, J.A., Clark, T.G., Horn, D. Cathepsin-L can resist lysis by human serum in Trypanosoma brucei brucei. PLoS Pathog. 10, e1004130 (2014).
-
(2014)
PLoS Pathog
, vol.10
, pp. 1004130
-
-
Alsford, S.1
Currier, R.B.2
Guerra-Assuncao, J.A.3
Clark, T.G.4
Horn, D.5
-
9
-
-
0028009258
-
Axenic culture of African trypanosome bloodstream forms
-
Hirumi, H., Hirumi, K. Axenic culture of African trypanosome bloodstream forms. Parasitol. Today 10, 80-84 (1994).
-
(1994)
Parasitol. Today
, vol.10
, pp. 80-84
-
-
Hirumi, H.1
Hirumi, K.2
-
10
-
-
22244441812
-
The genome of the African trypanosome Trypanosoma brucei
-
Berriman, M. et al. The genome of the African trypanosome Trypanosoma brucei. Science 309, 416-422 (2005).
-
(2005)
Science
, vol.309
, pp. 416-422
-
-
Berriman, M.1
-
11
-
-
38749115393
-
Codon usage suggests that translational selection has a major impact on protein expression in trypanosomatids
-
Horn, D. Codon usage suggests that translational selection has a major impact on protein expression in trypanosomatids. BMC Genomics 9, 2 (2008).
-
(2008)
BMC Genomics
, vol.9
, pp. 2
-
-
Horn, D.1
-
12
-
-
34247506767
-
Tandem gene arrays in Trypanosoma brucei: Comparative phylogenomic analysis of duplicate sequence variation
-
Jackson, A.P. Tandem gene arrays in Trypanosoma brucei: comparative phylogenomic analysis of duplicate sequence variation. BMC Evol. Biol. 7, 54 (2007).
-
(2007)
BMC Evol. Biol.
, vol.7
, pp. 54
-
-
Jackson, A.P.1
-
13
-
-
0027176807
-
DNA-mediated transformation of bloodstream-form Trypanosoma brucei
-
Carruthers, V.B., van der Ploeg, L.H., Cross, G.A. DNA-mediated transformation of bloodstream-form Trypanosoma brucei. Nucleic Acids Res. 21, 2537-2538 (1993).
-
(1993)
Nucleic Acids Res.
, vol.21
, pp. 2537-2538
-
-
Carruthers, V.B.1
Van Der Ploeg, L.H.2
Cross, G.A.3
-
14
-
-
0028989439
-
Inducible gene expression in trypanosomes mediated by a prokaryotic repressor
-
Wirtz, E., Clayton, C. Inducible gene expression in trypanosomes mediated by a prokaryotic repressor. Science 268, 1179-1183 (1995).
-
(1995)
Science
, vol.268
, pp. 1179-1183
-
-
Wirtz, E.1
Clayton, C.2
-
15
-
-
0032410928
-
Double-stranded RNA induces mRNA degradation in Trypanosoma brucei
-
Ngo, H., Tschudi, C., Gull, K., Ullu, E. Double-stranded RNA induces mRNA degradation in Trypanosoma brucei. Proc. Natl. Acad. Sci. USA 95, 14687-14692 (1998).
-
(1998)
Proc. Natl. Acad. Sci. USA
, vol.95
, pp. 14687-14692
-
-
Ngo, H.1
Tschudi, C.2
Gull, K.3
Ullu, E.4
-
16
-
-
0033768371
-
Double-stranded RNA interference in Trypanosoma brucei using head-to-head promoters
-
LaCount, D.J., Bruse, S., Hill, K.L., Donelson, J.E. Double-stranded RNA interference in Trypanosoma brucei using head-to-head promoters. Mol. Biochem. Parasitol. 111, 67-76 (2000).
-
(2000)
Mol. Biochem. Parasitol.
, vol.111
, pp. 67-76
-
-
Lacount, D.J.1
Bruse, S.2
Hill, K.L.3
Donelson, J.E.4
-
17
-
-
0034704084
-
Inhibition of Trypanosoma brucei gene expression by RNA interference using an integratable vector with opposing T7 promoters
-
Wang, Z., Morris, J.C., Drew, M.E., Englund, P.T. Inhibition of Trypanosoma brucei gene expression by RNA interference using an integratable vector with opposing T7 promoters. J. Biol. Chem. 275, 40174-40179 (2000).
-
(2000)
J. Biol. Chem.
, vol.275
, pp. 40174-40179
-
-
Wang, Z.1
Morris, J.C.2
Drew, M.E.3
Englund, P.T.4
-
18
-
-
0037009442
-
Glycolysis modulates trypanosome glycoprotein expression as revealed by an RNAi library
-
Morris, J.C., Wang, Z., Drew, M.E., Englund, P.T. Glycolysis modulates trypanosome glycoprotein expression as revealed by an RNAi library. EMBO J. 21, 4429-4438 (2002).
-
(2002)
EMBO J.
, vol.21
, pp. 4429-4438
-
-
Morris, J.C.1
Wang, Z.2
Drew, M.E.3
Englund, P.T.4
-
19
-
-
4143079140
-
RNA interference for analysis of gene function in trypanosomatids
-
Motyka, S.A., Englund, P.T. RNA interference for analysis of gene function in trypanosomatids. Curr. Opin. Microbiol. 7, 362-368 (2004).
-
(2004)
Curr. Opin. Microbiol.
, vol.7
, pp. 362-368
-
-
Motyka, S.A.1
Englund, P.T.2
-
20
-
-
0037466382
-
RNAit: An automated web-based tool for the selection of RNAi targets in Trypanosoma brucei
-
Redmond, S., Vadivelu, J., Field, M.C. RNAit: an automated web-based tool for the selection of RNAi targets in Trypanosoma brucei. Mol. Biochem. Parasitol. 128, 115-118 (2003).
-
(2003)
Mol. Biochem. Parasitol.
, vol.128
, pp. 115-118
-
-
Redmond, S.1
Vadivelu, J.2
Field, M.C.3
-
21
-
-
33748856576
-
Chromosome-wide analysis of gene function by RNA interference in the African trypanosome
-
Subramaniam, C. et al. Chromosome-wide analysis of gene function by RNA interference in the African trypanosome. Eukaryotic Cell 5, 1539-1549 (2006).
-
(2006)
Eukaryotic Cell
, vol.5
, pp. 1539-1549
-
-
Subramaniam, C.1
-
22
-
-
27644517022
-
Tagging a T. Brucei RRNA locus improves stable transfection efficiency and circumvents inducible expression position effects
-
Alsford, S., Kawahara, T., Glover, L., Horn, D. Tagging a T. brucei RRNA locus improves stable transfection efficiency and circumvents inducible expression position effects. Mol. Biochem. Parasitol. 144, 142-148 (2005).
-
(2005)
Mol. Biochem. Parasitol.
, vol.144
, pp. 142-148
-
-
Alsford, S.1
Kawahara, T.2
Glover, L.3
Horn, D.4
-
23
-
-
11144304648
-
Multiplex analysis of RNA interference defects in Trypanosoma brucei
-
Alsford, S., Glover, L., Horn, D. Multiplex analysis of RNA interference defects in Trypanosoma brucei. Mol. Biochem. Parasitol. 139, 129-132 (2005).
-
(2005)
Mol. Biochem. Parasitol.
, vol.139
, pp. 129-132
-
-
Alsford, S.1
Glover, L.2
Horn, D.3
-
24
-
-
43349103776
-
Sequence homology and microhomology dominate chromosomal double-strand break repair in African trypanosomes
-
Glover, L., McCulloch, R., Horn, D. Sequence homology and microhomology dominate chromosomal double-strand break repair in African trypanosomes. Nucleic Acids Res. 36, 2608-2618 (2008).
-
(2008)
Nucleic Acids Res
, vol.36
, pp. 2608-2618
-
-
Glover, L.1
McCulloch, R.2
Horn, D.3
-
25
-
-
67349156677
-
Site-specific DNA double-strand breaks greatly increase stable transformation efficiency in Trypanosoma brucei
-
Glover, L., Horn, D. Site-specific DNA double-strand breaks greatly increase stable transformation efficiency in Trypanosoma brucei. Mol. Biochem. Parasitol. 166, 194-197 (2009).
-
(2009)
Mol. Biochem. Parasitol.
, vol.166
, pp. 194-197
-
-
Glover, L.1
Horn, D.2
-
26
-
-
73249130761
-
Simultaneous assay of every Salmonella typhi gene using one million transposon mutants
-
Langridge, G.C. et al. Simultaneous assay of every Salmonella typhi gene using one million transposon mutants. Genome Res. 19, 2308-2316 (2009).
-
(2009)
Genome Res
, vol.19
, pp. 2308-2316
-
-
Langridge, G.C.1
-
27
-
-
0344443749
-
The adenosine analog tubercidin inhibits glycolysis in Trypanosoma brucei as revealed by an RNA interference library
-
Drew, M.E. et al. The adenosine analog tubercidin inhibits glycolysis in Trypanosoma brucei as revealed by an RNA interference library. J. Biol. Chem. 278, 46596-46600 (2003).
-
(2003)
J. Biol. Chem.
, vol.278
, pp. 46596-46600
-
-
Drew, M.E.1
-
28
-
-
42449155428
-
A mechanism for cross-resistance to nifurtimox and benznidazole in trypanosomes
-
Wilkinson, S.R., Taylor, M.C., Horn, D., Kelly, J.M., Cheeseman, I. A mechanism for cross-resistance to nifurtimox and benznidazole in trypanosomes. Proc. Natl. Acad. Sci. USA 105, 5022-5027 (2008).
-
(2008)
Proc. Natl. Acad. Sci. USA
, vol.105
, pp. 5022-5027
-
-
Wilkinson, S.R.1
Taylor, M.C.2
Horn, D.3
Kelly, J.M.4
Cheeseman, I.5
-
29
-
-
84863585771
-
Aquaglyceroporin 2 controls susceptibility to melarsoprol and pentamidine in African trypanosomes
-
Baker, N. et al. Aquaglyceroporin 2 controls susceptibility to melarsoprol and pentamidine in African trypanosomes. Proc. Natl. Acad. Sci. USA 109, 10996-11001 (2012).
-
(2012)
Proc. Natl. Acad. Sci. USA
, vol.109
, pp. 10996-11001
-
-
Baker, N.1
-
30
-
-
84887310584
-
Aquaporin 2 mutations in Trypanosoma brucei gambiense field isolates correlate with decreased susceptibility to pentamidine and melarsoprol
-
Graf, F.E. et al. Aquaporin 2 mutations in Trypanosoma brucei gambiense field isolates correlate with decreased susceptibility to pentamidine and melarsoprol. PLoS Negl. Trop. Dis. 7, e2475 (2013).
-
(2013)
PLoS Negl. Trop. Dis.
, vol.7
, pp. 2475
-
-
Graf, F.E.1
-
31
-
-
84903465686
-
A genome-wide tethering screen reveals novel potential post-transcriptional regulators in Trypanosoma brucei
-
Erben, E.D., Fadda, A., Lueong, S., Hoheisel, J.D., Clayton, C. A genome-wide tethering screen reveals novel potential post-transcriptional regulators in Trypanosoma brucei. PLoS Pathog. 10, e1004178 (2014).
-
(2014)
PLoS Pathog
, vol.10
, pp. 1004178
-
-
Erben, E.D.1
Fadda, A.2
Lueong, S.3
Hoheisel, J.D.4
Clayton, C.5
-
32
-
-
78449245604
-
Retention and loss of RNA interference pathways in trypanosomatid protozoans
-
Lye, L.F. et al. Retention and loss of RNA interference pathways in trypanosomatid protozoans. PLoS Pathog. 6, e1001161 (2010).
-
(2010)
PLoS Pathog
, vol.6
, pp. 1001161
-
-
Lye, L.F.1
-
33
-
-
84892749369
-
Genetic screens in human cells using the CRISPR-Cas9 system
-
Wang, T., Wei, J.J., Sabatini, D.M., Lander, E.S. Genetic screens in human cells using the CRISPR-Cas9 system. Science 343, 80-84 (2014).
-
(2014)
Science
, vol.343
, pp. 80-84
-
-
Wang, T.1
Wei, J.J.2
Sabatini, D.M.3
Lander, E.S.4
-
34
-
-
0026725397
-
Electroporation in 'intracellular' buffer increases cell survival
-
van den Hoff, M.J., Moorman, A.F., Lamers, W.H. Electroporation in 'intracellular' buffer increases cell survival. Nucleic Acids Res. 20, 2902 (1992).
-
(1992)
Nucleic Acids Res
, vol.20
, pp. 2902
-
-
Van Den Hoff, M.J.1
Moorman, A.F.2
Lamers, W.H.3
-
35
-
-
47949091926
-
Single-locus targeting constructs for reliable regulated RNAi and transgene expression in Trypanosoma brucei
-
Alsford, S., Horn, D. Single-locus targeting constructs for reliable regulated RNAi and transgene expression in Trypanosoma brucei. Mol. Biochem. Parasitol. 161, 76-79 (2008).
-
(2008)
Mol. Biochem. Parasitol.
, vol.161
, pp. 76-79
-
-
Alsford, S.1
Horn, D.2
-
36
-
-
75249095274
-
DEGseq: An R package for identifying differentially expressed genes from RNA-seq data
-
Wang, L., Feng, Z., Wang, X., Zhang, X. DEGseq: an R package for identifying differentially expressed genes from RNA-seq data. Bioinformatics 26, 136-138 (2010).
-
(2010)
Bioinformatics
, vol.26
, pp. 136-138
-
-
Wang, L.1
Feng, Z.2
Wang, X.3
Zhang, X.4
-
37
-
-
0030612893
-
The Alamar Blue assay to determine drug sensitivity of African trypanosomes (T.B. Rhodesiense and T.B. Gambiense) in vitro
-
Raz, B., Iten, M., Grether-Buhler, Y., Kaminsky, R., Brun, R. The Alamar Blue assay to determine drug sensitivity of African trypanosomes (T.b. rhodesiense and T.b. gambiense) in vitro. Acta Tropica 68, 139-147 (1997).
-
(1997)
Acta Tropica
, vol.68
, pp. 139-147
-
-
Raz, B.1
Iten, M.2
Grether-Buhler, Y.3
Kaminsky, R.4
Brun, R.5
-
38
-
-
57049122389
-
A large genome center's improvements to the Illumina sequencing system
-
Quail, M.A. et al. A large genome center's improvements to the Illumina sequencing system. Nat. Methods 5, 1005-1010 (2008).
-
(2008)
Nat. Methods
, vol.5
, pp. 1005-1010
-
-
Quail, M.A.1
-
39
-
-
0038173771
-
Efficiency and specificity of RNA interference generated by intra-and intermolecular double stranded RNA in Trypanosoma brucei
-
Durand-Dubief, M., Kohl, L., Bastin, P. Efficiency and specificity of RNA interference generated by intra-and intermolecular double stranded RNA in Trypanosoma brucei. Mol. Biochem. Parasitol. 129, 11-21 (2003).
-
(2003)
Mol. Biochem. Parasitol.
, vol.129
, pp. 11-21
-
-
Durand-Dubief, M.1
Kohl, L.2
Bastin, P.3
-
40
-
-
84896398000
-
Discovery of unconventional kinetochores in kinetoplastids
-
Akiyoshi, B., Gull, K. Discovery of unconventional kinetochores in kinetoplastids. Cell 156, 1247-1258 (2014).
-
(2014)
Cell
, vol.156
, pp. 1247-1258
-
-
Akiyoshi, B.1
Gull, K.2
-
41
-
-
84893808671
-
Regulators of Trypanosoma brucei cell cycle progression and differentiation identified using a kinome-wide RNAi screen
-
Jones, N.G. et al. Regulators of Trypanosoma brucei cell cycle progression and differentiation identified using a kinome-wide RNAi screen. PLoS Pathog. 10, e1003886 (2014).
-
(2014)
PLoS Pathog
, vol.10
, pp. 1003886
-
-
Jones, N.G.1
-
42
-
-
84897402075
-
Genetic validation of aminoacyl-tRNA synthetases as drug targets in Trypanosoma brucei
-
Kalidas, S. et al. Genetic validation of aminoacyl-tRNA synthetases as drug targets in Trypanosoma brucei. Eukaryotic Cell 13, 504-516 (2014).
-
(2014)
Eukaryotic Cell
, vol.13
, pp. 504-516
-
-
Kalidas, S.1
-
43
-
-
78951481408
-
A molecular mechanism for eflornithine resistance in African trypanosomes
-
Vincent, I.M. et al. A molecular mechanism for eflornithine resistance in African trypanosomes. PLoS Pathog. 6, e1001204 (2010).
-
(2010)
PLoS Pathog
, vol.6
, pp. 1001204
-
-
Vincent, I.M.1
-
44
-
-
0037330751
-
Development of RNA interference revertants in Trypanosoma brucei cell lines generated with a double stranded RNA expression construct driven by two opposing promoters
-
Chen, Y., Hung, C.H., Burderer, T., Lee, G.S. Development of RNA interference revertants in Trypanosoma brucei cell lines generated with a double stranded RNA expression construct driven by two opposing promoters. Mol. Biochem. Parasitol. 126, 275-279 (2003).
-
(2003)
Mol. Biochem. Parasitol.
, vol.126
, pp. 275-279
-
-
Chen, Y.1
Hung, C.H.2
Burderer, T.3
Lee, G.S.4
-
45
-
-
1642431650
-
Integration of pZJM library plasmids into unexpected locations in the Trypanosoma brucei genome
-
Motyka, S.A., Zhao, Z., Gull, K., Englund, P.T. Integration of pZJM library plasmids into unexpected locations in the Trypanosoma brucei genome. Mol. Biochem. Parasitol. 134, 163-167 (2004).
-
(2004)
Mol. Biochem. Parasitol.
, vol.134
, pp. 163-167
-
-
Motyka, S.A.1
Zhao, Z.2
Gull, K.3
Englund, P.T.4
-
46
-
-
84859210032
-
Fast gapped-read alignment with Bowtie 2
-
Langmead, B., Salzberg, S.L. Fast gapped-read alignment with Bowtie 2. Nat. Methods 9, 357-359 (2012).
-
(2012)
Nat. Methods
, vol.9
, pp. 357-359
-
-
Langmead, B.1
Salzberg, S.L.2
-
47
-
-
68549104404
-
The Sequence Alignment/Map format and SAMtools
-
Li, H. et al. The Sequence Alignment/Map format and SAMtools. Bioinformatics 25, 2078-2079 (2009).
-
(2009)
Bioinformatics
, vol.25
, pp. 2078-2079
-
-
Li, H.1
-
48
-
-
0033635022
-
Artemis: Sequence visualization and annotation
-
Rutherford, K. et al. Artemis: sequence visualization and annotation. Bioinformatics 16, 944-945 (2000).
-
(2000)
Bioinformatics
, vol.16
, pp. 944-945
-
-
Rutherford, K.1
|