Tagging a T. brucei RRNA locus improves stable transfection efficiency and circumvents inducible expression position effects

Molecular and Biochemical Parasitology

Volumn 144, Issue 2, 2005, Pages 142-148

  Alsford, Sam ^a   Kawahara, Taemi ^a   Glover, Lucy ^a   Horn, David ^a  

^a LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE   (United Kingdom)

Author keywords

Bloodstream form; Functional genomics; GFP; Leaky; Position effect; RNAi; T7 RNA polymerase; Transfection

Indexed keywords

GREEN FLUORESCENT PROTEIN; RIBOSOME RNA; RNA POLYMERASE;

ARTICLE; CONTROLLED STUDY; GENE LOCUS; GENETIC TRANSFECTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RNA INTERFERENCE; TRYPANOSOMA BRUCEI;

ANIMALS; DNA-DIRECTED RNA POLYMERASES; PROMOTER REGIONS (GENETICS); RNA INTERFERENCE; RNA, PROTOZOAN; RNA, RIBOSOMAL; TRANSFECTION; TRYPANOSOMA BRUCEI BRUCEI; VIRAL PROTEINS;

MAMMALIA; TRYPANOSOMA BRUCEI;

EID: 27644517022     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2005.08.009     Document Type: Article

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