A large genome center's improvements to the Illumina sequencing system

Nature Methods

Volumn 5, Issue 12, 2008, Pages 1005-1010

  Quail, Michael A ^a   Kozarewa, Iwanka ^a   Smith, Frances ^a   Scally, Aylwyn ^a   Stephens, Philip J ^a   Durbin, Richard ^a   Swerdlow, Harold ^a   Turner, Daniel J ^a  

^a WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA; SINGLE STRANDED DNA;

ANALYZER; ARTICLE; BIOINFORMATICS; CONTROLLED STUDY; DNA CONTENT; DNA DENATURATION; DNA EXTRACTION; DNA FRAGMENTATION; DNA HYBRIDIZATION; DNA LIBRARY; DNA MODIFICATION; DNA REPAIR; DNA SEQUENCE; DNA TEMPLATE; GENE AMPLIFICATION; GENE TECHNOLOGY; GENOME SIZE; HIGH THROUGHPUT SCREENING; HUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SEQUENCE DATABASE;

EID: 57049122389     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth.1270     Document Type: Article

References (13)

1. Bentley, D.R. Whole-genome re-sequencing. Curr. Opin. Genet. Dev. 16, 545-552 (2006).
2. Mardis, E.R. The impact of next-generation sequencing technology on genetics. Trends Genet. 24, 133-141 (2008).
3. Smith, D.R. et al. Rapid whole-genome mutational profiling using next-generation sequencing technologies. Genome Res. 18, 1638-1642 (2008).
4. Surzycki, S. DNA sequencing. in Basic Techniques in Molecular Biology 377-380 (Springer-Verlag, Berlin, 2000).
5. Albert, T.J. et al. Direct selection of human genomic loci by microarray hybridization. Nat. Methods 4, 903-905 (2007).
6. Hodges, E. et al. Genome-wide in situ exon capture for selective resequencing. Nat. Genet. 39, 1522-1527 (2007).
7. Sambrook, J., Fritsch, E. & Maniatis, T. Molecular Cloning: A Laboratory Manual, (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1989).
8. Smith, D. & Malek, J. Asymmetrical adapters and uses thereof. US patent 0172839 (2007).
9. Riley, J. et al. A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones. Nucleic Acids Res. 18, 2887-2890 (1990).
10. Hawkins, T.L., O'Connor-Morin, T., Roy, A. & Santillan, C. DNA purification and isolation using a solid-phase. Nucleic Acids Res. 22, 4543-4544 (1994).
11. Meyer, M. et al. From micrograms to picograms: Quantitative PCR reduces the material demands of high-throughput sequencing. Nucleic Acids Res. 36, e5 (2008).
12. Thomas, R. The denaturation of DNA. Gene 135, 77-79 (1993).
13. Mandel, M. & Marmur, J. Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol. 12, 195-206 (1968).