Generating targeted libraries by the combinatorial incorporation of synthetic oligonucleotides during gene shuffling (ISOR)

Methods in Molecular Biology

Volumn 1179, Issue , 2014, Pages 129-137

  Rockah Shmuel, Liat ^a   Tawfik, Dan S ^a   Goldsmith, Moshe ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

Author keywords

Combinatorial library; Designed library; Directed evolution; Gene shuffling; Protein engineering; Saturation library; Targeted mutations

Indexed keywords

DNA FRAGMENT; OLIGONUCLEOTIDE;

ARTICLE; COMBINATORIAL LIBRARY; DNA FRAGMENTATION; DNA HYBRIDIZATION; DNA TEMPLATE; GENE MUTATION; GENE SHUFFLING; GENETIC VARIABILITY; INCORPORATING SYNTHETIC OLIGONUCLEOTIDES VIA GENE REASSEMBLY; MOLECULAR CLONING; MOLECULAR EVOLUTION; MOLECULAR GENETICS; PH; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ENGINEERING; SITE DIRECTED MUTAGENESIS; DIRECTED MOLECULAR EVOLUTION; GENETICS; PROCEDURES;

DIRECTED MOLECULAR EVOLUTION; MUTAGENESIS, SITE-DIRECTED; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING;

EID: 84925883703     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1053-3_8     Document Type: Article

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