Reconstructed evolutionary adaptive paths give polymerases accepting reversible terminators for sequencing and SNP detection

Proceedings of the National Academy of Sciences of the United States of America

Volumn 107, Issue 5, 2010, Pages 1948-1953

  Chen, Fei ^a,b   Gauchera, Eric A ^a,c   Leal, Nicole A ^a,b   Hutter, Daniel ^a,b   Havemann, Stephanie A ^a   Govindarajan, Sridhar ^d   Ortlund, Eric A ^e   Benner, Steven A ^a,b  

^a FOUNDATION FOR APPLIED MOLECULAR EVOLUTION   (United States)

^b Westheimer Institute for Science and Technology TWIST   (United States)

^c GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

^d DNA20 Inc   (United States)

^e EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Dna polymerases; Evolutionary analysis; Sequencing technology; Single nucleotide polymorphisms; Synthetic biology

Indexed keywords

HYDROXIDE; NITROGEN; NUCLEOSIDE TRIPHOSPHATE; OXYGEN; TAQ POLYMERASE;

ARTICLE; CATALYSIS; CHEMICAL BOND; CRYSTALLOGRAPHY; DNA DETERMINATION; GENE SEQUENCE; GENE STRUCTURE; MOLECULAR EVOLUTION; PRIORITY JOURNAL; PROTEIN FUNCTION; SINGLE NUCLEOTIDE POLYMORPHISM; SUBSTITUTION REACTION;

AMINO ACID SUBSTITUTION; BASE SEQUENCE; CATALYTIC DOMAIN; DNA PRIMERS; EVOLUTION, MOLECULAR; GENETIC VARIATION; MODELS, MOLECULAR; POLYMORPHISM, SINGLE NUCLEOTIDE; SEQUENCE ANALYSIS, DNA; SUBSTRATE SPECIFICITY; TAQ POLYMERASE;

EID: 76649090003     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0908463107     Document Type: Article

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