Affinity purification of in vitro transcribed RNA with homogeneous ends using a 3′-aribo tag

Methods in Enzymology

Volumn 549, Issue C, 2014, Pages 49-84

  Di Tomasso, Geneviève ^a   Salvail Lacoste, Alix ^a   Bouvette, Jonathan ^a   Omichinski, James G ^a   Legault, Pascale ^a  

^a UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

Affinity purification of RNA; ARiBo tag; CRISPR; CRISPR specific nuclease; Hammerhead ribozyme; Lambda boxB; Lambda N protein glmS ribozyme; Riboswitch; RNA; RNA polymerase; T7

Indexed keywords

HAMMERHEAD RIBOZYME; HYBRID PROTEIN; PLASMID DNA; RIBONUCLEASE; RNA POLYMERASE; AFFINITY LABELING; BACTERIOPHAGE T7 RNA POLYMERASE; DNA DIRECTED RNA POLYMERASE; RIBOZYME; RNA; VIRUS PROTEIN;

3' ARIBO TAG; AFFINITY PURIFICATION; ARTICLE; BACTERIAL CELL; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA TEMPLATE; GENETIC PROCEDURES; IN VITRO STUDY; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; QUANTITATIVE ANALYSIS; RNA CLEAVAGE; RNA PURIFICATION; RNA TRANSCRIPTION; AFFINITY LABELING; AMINO ACID SEQUENCE; BACILLUS ANTHRACIS; BACTERIUM; CHEMISTRY; CULTURE TECHNIQUE; ENTEROBACTERIA PHAGE LAMBDA; ENTEROBACTERIA PHAGE T7; GENETIC TRANSCRIPTION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROCEDURES;

AFFINITY LABELS; AMINO ACID SEQUENCE; BACILLUS ANTHRACIS; BACTERIA; BACTERIOPHAGE LAMBDA; BACTERIOPHAGE T7; BASE SEQUENCE; CELL CULTURE TECHNIQUES; CLONING, MOLECULAR; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA-DIRECTED RNA POLYMERASES; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; RNA; RNA, CATALYTIC; TRANSCRIPTION, GENETIC; VIRAL PROTEINS;

EID: 84925438125     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-801122-5.00003-9     Document Type: Chapter

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