Affinity purification of T7 RNA transcripts with homogeneous ends using ARiBo and CRISPR tags

RNA

Volumn 19, Issue 7, 2013, Pages 1003-1014

  Salvail Lacoste, Alix ^a   Di Tomasso, Geneviève ^a   Piette, Benjamin L ^a   Legault, Pascale ^a  

^a UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

5 heterogeneity; Affinity purification of RNA; ARiBo tag; CRISPR tag; Cse3 endoribonuclease; T7 RNA polymerase

Indexed keywords

DOUBLE STRANDED RNA; NUCLEOTIDE; PROTEIN CSE3; RIBONUCLEASE; RIBOZYME; RNA POLYMERASE; RNA PRECURSOR; SINGLE STRANDED RNA; T7 RNA POLYMERASE; UNCLASSIFIED DRUG;

ACTIVATABLE RIBOZYME THE GLMS RIBOZYME AND THE LAMBDABOXB RNA TAG; ARTICLE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; GENETIC HETEROGENEITY; MUTANT; NEUROSPORA; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; RNA CLEAVAGE; RNA PURIFICATION; RNA SEQUENCE; RNA TRANSCRIPTION;

5′ HETEROGENEITY; AFFINITY PURIFICATION OF RNA; ARIBO TAG; CRISPR TAG; CSE3 ENDORIBONUCLEASE; T7 RNA POLYMERASE;

AFFINITY LABELS; BACTERIOPHAGE T7; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; DNA-DIRECTED RNA POLYMERASES; GENETIC HETEROGENEITY; INVERTED REPEAT SEQUENCES; NEUROSPORA; NUCLEIC ACID CONFORMATION; PLASMIDS; PROMOTER REGIONS, GENETIC; RECOMBINANT FUSION PROTEINS; RNA CLEAVAGE; RNA STABILITY; RNA, CATALYTIC; RNA, FUNGAL; RNA, SPLICED LEADER; RNA, VIRAL; THERMUS THERMOPHILUS; TRANSCRIPTION, GENETIC; VIRAL PROTEINS;

NEUROSPORA;

EID: 84879323584     PISSN: 13558382     EISSN: 14699001     Source Type: Journal    
DOI: 10.1261/rna.037432.112     Document Type: Article

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