Relaxed rotational and scrunching changes in P266L mutant of T7 RNA polymerase reduce short abortive RNAs while delaying transition into elongation

PLoS ONE

Volumn 9, Issue 3, 2014, Pages

  Tang, Guo Qing ^a   Nandakumar, Divya ^a   Bandwar, Rajiv P ^a   Lee, Kyung Suk ^b   Roy, Rahul ^c   Ha, Taekjip ^b,c,d   Patel, Smita S ^a  

^a RUTGERS ROBERT WOOD JOHNSON MEDICAL SCHOOL   (United States)

^b University of Illinois at Urbana Champaign   (United States)

^c UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^d Howard Hughes Medical Institutes   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GUANOSINE TRIPHOSPHATE; LEUCINE; PROLINE; RNA; RNA POLYMERASE; BACTERIOPHAGE T7 RNA POLYMERASE; DNA DIRECTED RNA POLYMERASE; MESSENGER RNA; MUTANT PROTEIN; PROTEIN BINDING; VIRUS DNA; VIRUS PROTEIN; VIRUS RNA;

ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DNA RNA HYBRIDIZATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; MUTATIONAL ANALYSIS; PROMOTER REGION; PROTEIN DOMAIN; RNA SYNTHESIS; RNA TRANSCRIPTION; TRANSCRIPTION INITIATION; WILD TYPE; AMINO ACID SEQUENCE; BIOSYNTHESIS; CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; PROTEIN FOLDING; PROTEIN TERTIARY STRUCTURE; ROTATION; TRANSCRIPTION ELONGATION;

AMINO ACID SEQUENCE; DNA, VIRAL; DNA-DIRECTED RNA POLYMERASES; GUANOSINE TRIPHOSPHATE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTANT PROTEINS; MUTATION; PROMOTER REGIONS, GENETIC; PROTEIN BINDING; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; RNA, MESSENGER; RNA, VIRAL; ROTATION; TRANSCRIPTION ELONGATION, GENETIC; VIRAL PROTEINS;

EID: 84898635934     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0091859     Document Type: Article

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