Theoretical study of enzymatic catalysis explains why the trapped covalent intermediate in the E303C mutant of glycosyltransferase GTB was not detected in the wild-type enzyme

Glycobiology

Volumn 25, Issue 1, 2015, Pages 3-7

  Bobovská, Adela ^a,b   Tvaroška, Igor ^a,c   Kóňa, Juraj ^a  

^a INSTITUTE OF CHEMISTRY   (Slovakia)

^b Physics and Informatics   (Slovakia)

^c The Philosopher University in Nitra Geoinformatics and Regional Development   (Slovakia)

Author keywords

glycosyl enzyme intermediate; kinetic isotope effect; mechanism of glycosyltransferases; molecular modeling; structure of transition state

Indexed keywords

ALPHA (1,3) GALACTOSYLTRANSFERASE; CARBON; GALACTOSYLTRANSFERASE; ION; ISOTOPE; NUCLEOPHILE; OXOCARBENIUM ION; OXYGEN; UNCLASSIFIED DRUG; CYSTEINE; GLUTAMIC ACID; N-ACETYLLACTOSAMINIDE ALPHA-1,3-GALACTOSYLTRANSFERASE; PROTEIN BINDING;

ARTICLE; CATALYSIS; CHEMICAL BOND; CONFORMATION; CRYSTAL STRUCTURE; ENERGY; ENZYMATIC CATALYSIS; GLYCOSIDIC BOND; HUMAN; MASS SPECTROMETRY; METHODOLOGY; NUCLEOPHILICITY; POTENTIAL ENERGY SURFACE; PRIORITY JOURNAL; PROTON TRANSPORT; RATE CONSTANT; SURFACE PROPERTY; THEORETICAL STUDY; WILD TYPE; AMINO ACID SUBSTITUTION; BINDING SITE; BIOCATALYSIS; CHEMICAL MODEL; CHEMISTRY; ENZYME SPECIFICITY; GENETICS; KINETICS; MUTATION; PROTEIN CONFORMATION; QUANTUM THEORY; THERMODYNAMICS;

AMINO ACID SUBSTITUTION; BINDING SITES; BIOCATALYSIS; CYSTEINE; GALACTOSYLTRANSFERASES; GLUTAMIC ACID; HUMANS; KINETICS; MASS SPECTROMETRY; MODELS, CHEMICAL; MUTATION; PROTEIN BINDING; PROTEIN CONFORMATION; QUANTUM THEORY; SUBSTRATE SPECIFICITY; THERMODYNAMICS;

EID: 84924942777     PISSN: 09596658     EISSN: 14602423     Source Type: Journal    
DOI: 10.1093/glycob/cwu085     Document Type: Article

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