Dissection of the pH dependence of inhibitor binding energies for an aspartic protease: Direct measurement of the protonation states of the catalytic aspartic acid residues

Biochemistry

Volumn 36, Issue 51, 1997, Pages 16166-16172

  Xie, Dong ^a   Gulnik, Sergei ^a   Collins, Laura ^a   Gustchina, Elena ^a   Suvorov, Leonid ^a   Erickson, John W ^a  

^a SAIC FREDERICK INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC ACID; ASPARTIC PROTEINASE; PEPSTATIN; PLASMEPSIN II; UNCLASSIFIED DRUG;

ARTICLE; CALORIMETRY; DRUG DESIGN; ENERGY TRANSFER; ENZYME MECHANISM; NONHUMAN; PH; PLASMODIUM FALCIPARUM; PRIORITY JOURNAL; PROTON TRANSPORT; THERMODYNAMICS;

ANIMALS; ASPARTIC ENDOPEPTIDASES; CALORIMETRY; CATHEPSIN D; CIRCULAR DICHROISM; HYDROGEN-ION CONCENTRATION; MODELS, MOLECULAR; PEPSTATINS; PLASMODIUM FALCIPARUM; PROTEASE INHIBITORS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; PROTONS; THERMODYNAMICS;

PLASMODIUM FALCIPARUM;

EID: 0031423107     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi971550l     Document Type: Article

References (13)

1. Goldberg, D. E., Slater, A. F. G., Cerami, A., & Henderson, G. B. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 2931-2935.
2. Silva, A., Lee, A., Gulnik, S., Majer, P., Collins, J., Bhat, T., Collins, P., Cachau, R., Luker, K., Gluzman, I., Francis, S., Oksman, A., Goldberg, D., & Erickson, J. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 10034-10039.
3. Baldwin, E. T., Bhat, T. N., Gulnik, S., Hosur, M. V., Sowder, R. C, II, Cachau, R. E., Collins, J., Silva, A. M., & Erickson, J. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 6796-6800.
4. Bailey, D., Cooper, J., Tickle, I., Veepandian, B., Blundell, T., Atrash, B., Jones, D., & Szelke, M. (1993) Biochem. J. 289, 363-371.
5. Xie, D., Gulnik, S., Collins, L., Gustchina, E., Bhat, T. N., & Erickson, J. W. (1997) in Structure and Function of Aspartic Proteinases: Retroviral and Cellular Enzymes (James, M., Ed.) Plenum, New York (in press).
6. Fruton, J. S. (1976) Adv. Enzymol. Relat. Areas Mol. Biol. 44, 1-36.
7. Hofmann, T., Hodges, R., & James, M. (1984) Biochemistry 23, 635-643.
8. Hyland, L., Tomaszek, T., & Meek, T. (1991) Biochemistry 30, 8454-8463.
9. Lin, Y., Fusek, M., Lin, X., Hartsuck, J., Kezdy, F., & Tang, J. (1992) J. Biol. Chem. 267, 18413-18418.
10. Gomez, J., & Freire, E. (1995) J. Mol. Biol. 252, 337-350.
11. Wiseman, T., Williston, S., Brandt, J., & Lin, L. (1989) Anal. Biochem. 179, 131-135.
12. Johnson, M. L. (1983) Biophys. J. 44, 101-106.
13. Johnson, M. L., & Frasier, S. G. (1985) Methods Enzymol. 117, 301-342.