Site-directed mutagenesis of glutamate 317 of bovine α-1,3Galactosyltransferase and its effect on enzyme activity: Implications for reaction mechanism

Biochimica et Biophysica Acta - General Subjects

Volumn 1770, Issue 8, 2007, Pages 1266-1273

  Molina, Patricia ^a   Knegtel, Ronald M A ^b   Macher, Bruce A ^a  

^a SAN FRANCISCO STATE UNIVERSITY   (United States)

^b VERTEX PHARMACEUTICALS   (United States)

Author keywords

1,3Galactosyltransferase; Double displacement reaction mechanism; Modeling; Site directed mutagenesis

Indexed keywords

ALANINE; ALPHA 1,3 GALACTOSYLTRANSFERASE; ASPARTIC ACID; CYSTEINE; GALACTOSE; GLUTAMIC ACID; HISTIDINE;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; ENZYME ACTIVITY; GENE MUTATION; MOLECULAR MODEL; PRIORITY JOURNAL; REACTION ANALYSIS; SITE DIRECTED MUTAGENESIS;

ANIMALS; BINDING SITES; CATTLE; GALACTOSYLTRANSFERASES; GLUTAMIC ACID; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; SUBSTRATE SPECIFICITY;

BOVINAE;

EID: 34347350226     PISSN: 03044165     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbagen.2007.04.012     Document Type: Article

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