Asymmetry of the Budding Yeast Tem1 GTPase at Spindle Poles Is Required for Spindle Positioning But Not for Mitotic Exit

PLoS Genetics

Volumn 11, Issue 2, 2015, Pages 1-29

  Scarfone, Ilaria ^a,b   Venturetti, Marianna ^b   Hotz, Manuel ^c   Lengefeld, Jette ^c   Barral, Yves ^c   Piatti, Simonetta ^a,b  

^a Centre National de la Recherche Scientifique   (France)

^b UNIVERSITY OF MILANO BICOCCA   (Italy)

^c ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

BFA1 GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN; BUB2 GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN; TEM1 GUANOSINE TRIPHOSPHATASE; UNCLASSIFIED DRUG; BFA1 PROTEIN, S CEREVISIAE; BUB2 PROTEIN, S CEREVISIAE; CELL CYCLE PROTEIN; CYTOSKELETON PROTEIN; GLUTAMINE; KAR9 PROTEIN, S CEREVISIAE; MONOMERIC GUANINE NUCLEOTIDE BINDING PROTEIN; NUCLEAR PROTEIN; SACCHAROMYCES CEREVISIAE PROTEIN; TEM1 PROTEIN, S CEREVISIAE;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ANALYSIS; ENZYME LOCALIZATION; HYDROLYSIS; MITOSIS; MITOTIC EXIT NETWORK; NONHUMAN; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE; SPINDLE POLE; SPINDLE POSITION CHECKPOINT; CELL CYCLE; CELL POLARITY; CYTOKINESIS; GENE EXPRESSION REGULATION; GENETICS; METABOLISM;

SACCHAROMYCETALES;

CELL CYCLE; CELL CYCLE PROTEINS; CELL POLARITY; CYTOKINESIS; CYTOSKELETAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; GLUTAMINE; GTP PHOSPHOHYDROLASES; MITOSIS; MONOMERIC GTP-BINDING PROTEINS; NUCLEAR PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SPINDLE POLES;

EID: 84924425807     PISSN: 15537390     EISSN: 15537404     Source Type: Journal    
DOI: 10.1371/journal.pgen.1004938     Document Type: Article

References (84)

1. Inaba M, Yamashita YM, (2012) Asymmetric stem cell division: precision for robustness. Cell Stem Cell 11: 461–469. doi: 10.1016/j.stem.2012.09.003 23040475
2. Knoblich JA, (2010) Asymmetric cell division: recent developments and their implications for tumour biology. Nat Rev Mol Cell Biol 11: 849–860. doi: 10.1038/nrm3010 21102610
3. Li R, (2013) The art of choreographing asymmetric cell division. Dev Cell 25: 439–450. doi: 10.1016/j.devcel.2013.05.003 23763946
4. Gonzalez C, (2007) Spindle orientation, asymmetric division and tumour suppression in Drosophila stem cells. Nat Rev Genet 8: 462–472. doi: 10.1038/nrg2103 17510666
5. Siller KH, Doe CQ, (2009) Spindle orientation during asymmetric cell division. Nat Cell Biol 11: 365–374. doi: 10.1038/ncb0409-365 19337318
6. Caydasi AK, Ibrahim B, Pereira G, (2010) Monitoring spindle orientation: Spindle position checkpoint in charge. Cell Div 5: 28. doi: 10.1186/1747-1028-5-28 21143992
7. Yamashita YM, Yuan H, Cheng J, Hunt AJ, (2010) Polarity in stem cell division: asymmetric stem cell division in tissue homeostasis. Cold Spring Harb Perspect Biol 2: a001313. doi: 10.1101/cshperspect.a001313 20182603
8. Moore JK, Cooper JA, (2010) Coordinating mitosis with cell polarity: Molecular motors at the cell cortex. Semin Cell Dev Biol 21: 283–289. doi: 10.1016/j.semcdb.2010.01.020 20109571
9. Fraschini R, Venturetti M, Chiroli E, Piatti S, (2008) The spindle position checkpoint: how to deal with spindle misalignment during asymmetric cell division in budding yeast. Biochem Soc Trans 36: 416–420. doi: 10.1042/BST0360416 18481971
10. Krapp A, Gulli MP, Simanis V, (2004) SIN and the art of splitting the fission yeast cell. Curr Biol 14: R722–730. doi: 10.1016/j.cub.2004.08.049 15341766
11. Stegmeier F, Amon A, (2004) Closing Mitosis: The Functions of the Cdc14 Phosphatase and Its Regulation. Annu Rev Genet 38: 203–232. doi: 10.1146/annurev.genet.38.072902.093051 15568976
12. Visintin R, Craig K, Hwang ES, Prinz S, Tyers M, et al. (1998) The phosphatase Cdc14 triggers mitotic exit by reversal of Cdk- dependent phosphorylation. Mol Cell 2: 709–718. doi: 10.1016/S1097-2765(00)80286-5 9885559
13. Shou W, Seol JH, Shevchenko A, Baskerville C, Moazed D, et al. (1999) Exit from mitosis is triggered by Tem1-dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex. Cell 97: 233–244. doi: 10.1016/S0092-8674(00)80733-3 10219244
14. Visintin R, Hwang ES, Amon A, (1999) Cfi1 prevents premature exit from mitosis by anchoring Cdc14 phosphatase in the nucleolus [see comments]. Nature 398: 818–823. doi: 10.1038/19775 10235265
15. Stegmeier F, Visintin R, Amon A, (2002) Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function in a network that controls Cdc14 localization during early anaphase. Cell 108: 207–220. doi: 10.1016/S0092-8674(02)00618-9 11832211
16. Queralt E, Lehane C, Novak B, Uhlmann F, (2006) Downregulation of PP2A(Cdc55) phosphatase by separase initiates mitotic exit in budding yeast. Cell 125: 719–732. doi: 10.1016/j.cell.2006.03.038 16713564
17. D’Amours D, Amon A, (2004) At the interface between signaling and executing anaphase--Cdc14 and the FEAR network. Genes Dev 18: 2581–2595. doi: 10.1101/gad.1247304 15520278
18. Hotz M, Leisner C, Chen D, Manatschal C, Wegleiter T, et al. (2012) Spindle pole bodies exploit the mitotic exit network in metaphase to drive their age-dependent segregation. Cell 148: 958–972. doi: 10.1016/j.cell.2012.01.041 22385961
19. Liakopoulos D, Kusch J, Grava S, Vogel J, Barral Y, (2003) Asymmetric loading of Kar9 onto spindle poles and microtubules ensures proper spindle alignment. Cell 112: 561–574. doi: 10.1016/S0092-8674(03)00119-3 12600318
20. Pereira G, Schiebel E, (2001) The role of the yeast spindle pole body and the mammalian centrosome in regulating late mitotic events. Curr Opin Cell Biol 13: 762–769. doi: 10.1016/S0955-0674(00)00281-7 11698194
21. Gruneberg U, Campbell K, Simpson C, Grindlay J, Schiebel E, (2000) Nud1p links astral microtubule organization and the control of exit from mitosis [In Process Citation]. Embo J 19: 6475–6488. doi: 10.1093/emboj/19.23.6475 11101520
22. Valerio-Santiago M, Monje-Casas F, (2011) Tem1 localization to the spindle pole bodies is essential for mitotic exit and impairs spindle checkpoint function. J Cell Biol 192: 599–614. doi: 10.1083/jcb.201007044 21321099
23. Bourne HR, Sanders DA, McCormick F, (1990) The GTPase superfamily: a conserved switch for diverse cell functions. Nature 348: 125–132. doi: 10.1038/348125a0 2122258
24. Vetter IR, Wittinghofer A, (2001) The guanine nucleotide-binding switch in three dimensions. Science 294: 1299–1304. doi: 10.1126/science.1062023 11701921
25. Fraschini R, D’Ambrosio C, Venturetti M, Lucchini G, Piatti S, (2006) Disappearance of the budding yeast Bub2-Bfa1 complex from the mother-bound spindle pole contributes to mitotic exit. J Cell Biol 172: 335–346. doi: 10.1083/jcb.200507162 16449187
26. Geymonat M, Spanos A, Smith SJ, Wheatley E, Rittinger K, et al. (2002) Control of mitotic exit in budding yeast. In vitro regulation of Tem1 GTPase by Bub2 and Bfa1. J Biol Chem 277: 28439–28445. doi: 10.1074/jbc.M202540200 12048186
27. Bourne HR, Sanders DA, McCormick F, (1991) The GTPase superfamily: conserved structure and molecular mechanism. Nature 349: 117–127. doi: 10.1038/349117a0 1898771
28. Schweins T, Wittinghofer A, (1994) GTP-binding proteins. Structures, interactions and relationships. Curr Biol 4: 547–550. doi: 10.1016/S0960-9822(00)00122-6 7922378
29. Neuwald AF, (1997) A shared domain between a spindle assembly checkpoint protein and Ypt/Rab-specific GTPase-activators. Trends Biochem Sci 22: 243–244. doi: 10.1016/S0968-0004(97)01073-6 9255064
30. Geymonat M, Spanos A, de Bettignies G, Sedgwick SG, (2009) Lte1 contributes to Bfa1 localization rather than stimulating nucleotide exchange by Tem1. J Cell Biol 187: 497–511. doi: 10.1083/jcb.200905114 19948498
31. Ro HS, Song S, Lee KS, (2002) Bfa1 can regulate Tem1 function independently of Bub2 in the mitotic exit network of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 99: 5436–5441. doi: 10.1073/pnas.062059999 11959999
32. Maekawa H, Priest C, Lechner J, Pereira G, Schiebel E, (2007) The yeast centrosome translates the positional information of the anaphase spindle into a cell cycle signal. J Cell Biol 179: 423–436. doi: 10.1083/jcb.200705197 17967947
33. Chan LY, Amon A, (2010) Spindle position is coordinated with cell-cycle progression through establishment of mitotic exit-activating and -inhibitory zones. Mol Cell 39: 444–454. doi: 10.1016/j.molcel.2010.07.032 20705245
34. D’Aquino KE, Monje-Casas F, Paulson J, Reiser V, Charles GM, et al. (2005) The protein kinase Kin4 inhibits exit from mitosis in response to spindle position defects. Mol Cell 19: 223–234. doi: 10.1016/j.molcel.2005.06.005 16039591
35. Pereira G, Schiebel E, (2005) Kin4 kinase delays mitotic exit in response to spindle alignment defects. Mol Cell 19: 209–221. doi: 10.1016/j.molcel.2005.05.030 16039590
36. Caydasi AK, Pereira G, (2009) Spindle alignment regulates the dynamic association of checkpoint proteins with yeast spindle pole bodies. Dev Cell 16: 146–156. doi: 10.1016/j.devcel.2008.10.013 19154725
37. Frenz LM, Lee SE, Fesquet D, Johnston LH, (2000) The budding yeast Dbf2 protein kinase localises to the centrosome and moves to the bud neck in late mitosis. J Cell Sci 113 Pt 19: 3399–3408. 10984431
38. Molk JN, Schuyler SC, Liu JY, Evans JG, Salmon ED, et al. (2004) The differential roles of budding yeast Tem1p, Cdc15p, and Bub2p protein dynamics in mitotic exit. Mol Biol Cell 15: 1519–1532. doi: 10.1091/mbc.E03-09-0708 14718561
39. Monje-Casas F, Amon A, (2009) Cell polarity determinants establish asymmetry in MEN signaling. Dev Cell 16: 132–145. doi: 10.1016/j.devcel.2008.11.002 19154724
40. Pereira G, Hofken T, Grindlay J, Manson C, Schiebel E, (2000) The Bub2p spindle checkpoint links nuclear migration with mitotic exit. Mol Cell 6: 1–10. doi: 10.1016/S1097-2765(05)00017-1 10949022
41. Rock JM, Amon A, (2011) Cdc15 integrates Tem1 GTPase-mediated spatial signals with Polo kinase-mediated temporal cues to activate mitotic exit. Genes Dev 25: 1943–1954. doi: 10.1101/gad.17257711 21937712
42. Rock JM, Lim D, Stach L, Ogrodowicz RW, Keck JM, et al. (2013) Activation of the Yeast Hippo Pathway by Phosphorylation-Dependent Assembly of Signaling Complexes. Science 340: 871–875. doi: 10.1126/science.1235822 23579499
43. Visintin R, Amon A, (2001) Regulation of the mitotic exit protein kinases cdc15 and dbf2. Mol Biol Cell 12: 2961–2974. doi: 10.1091/mbc.12.10.2961 11598184
44. Xu S, Huang HK, Kaiser P, Latterich M, Hunter T, (2000) Phosphorylation and spindle pole body localization of the Cdc15p mitotic regulatory protein kinase in budding yeast. Curr Biol 10: 329–332. doi: 10.1016/S0960-9822(00)00382-1 10744974
45. Cerutti L, Simanis V, (1999) Asymmetry of the spindle pole bodies and spg1p GAP segregation during mitosis in fission yeast. J Cell Sci 112 (Pt 14): 2313–2321. 10381387
46. Li C, Furge KA, Cheng QC, Albright CF, (2000) Byr4 localizes to spindle-pole bodies in a cell cycle-regulated manner to control Cdc7 localization and septation in fission yeast. J Biol Chem 275: 14381–14387. doi: 10.1074/jbc.275.19.14381 10799520
47. Sohrmann M, Schmidt S, Hagan I, Simanis V, (1998) Asymmetric segregation on spindle poles of the Schizosaccharomyces pombe septum-inducing protein kinase Cdc7p. Genes Dev 12: 84–94. doi: 10.1101/gad.12.1.84 9420333
48. Pan X, Eathiraj S, Munson M, Lambright DG, (2006) TBC-domain GAPs for Rab GTPases accelerate GTP hydrolysis by a dual-finger mechanism. Nature 442: 303–306. doi: 10.1038/nature04847 16855591
49. Miller RK, Rose MD, (1998) Kar9p is a novel cortical protein required for cytoplasmic microtubule orientation in yeast. J Cell Biol 140: 377–390. doi: 10.1083/jcb.140.2.377 9442113
50. Yeh E, Skibbens RV, Cheng JW, Salmon ED, Bloom K, (1995) Spindle dynamics and cell cycle regulation of dynein in the budding yeast, Saccharomyces cerevisiae. J Cell Biol 130: 687–700. doi: 10.1083/jcb.130.3.687 7622568
51. Hu F, Wang Y, Liu D, Li Y, Qin J, et al. (2001) Regulation of the Bub2/Bfa1 GAP complex by Cdc5 and cell cycle checkpoints. Cell 107: 655–665. doi: 10.1016/S0092-8674(01)00580-3 11733064
52. Valerio-Santiago M, de Los Santos-Velazquez AI, Monje-Casas F, (2013) Inhibition of the mitotic exit network in response to damaged telomeres. PLoS Genet 9: e1003859. doi: 10.1371/journal.pgen.1003859 24130507
53. Asakawa K, Yoshida S, Otake F, Toh EA, (2001) A Novel Functional Domain of Cdc15 Kinase Is Required for Its Interaction With Tem1 GTPase in Saccharomyces cerevisiae. Genetics 157: 1437–1450. 11290702
54. Schmidt S, Sohrmann M, Hofmann K, Woollard A, Simanis V, (1997) The Spg1p GTPase is an essential, dosage-dependent inducer of septum formation in Schizosaccharomyces pombe. Genes Dev 11: 1519–1534. doi: 10.1101/gad.11.12.1519 9203579
55. Bi E, Maddox P, Lew DJ, Salmon ED, McMillan JN, et al. (1998) Involvement of an actomyosin contractile ring in Saccharomyces cerevisiae cytokinesis. J Cell Biol 142: 1301–1312. doi: 10.1083/jcb.142.5.1301 9732290
56. Park SY, Cable AE, Blair J, Stockstill KE, Shannnon KB, (2009) Bub2 regulation of cytokinesis and septation in budding yeast. BMC Cell Biol 10: 43. doi: 10.1186/1471-2121-10-43 19490645
57. Kahana JA, Schlenstedt G, Evanchuk DM, Geiser JR, Hoyt MA, et al. (1998) The yeast dynactin complex is involved in partitioning the mitotic spindle between mother and daughter cells during anaphase B. Mol Biol Cell 9: 1741–1756. doi: 10.1091/mbc.9.7.1741 9658168
58. Collins SR, Miller KM, Maas NL, Roguev A, Fillingham J, et al. (2007) Functional dissection of protein complexes involved in yeast chromosome biology using a genetic interaction map. Nature 446: 806–810. doi: 10.1038/nature05649 17314980
59. Costanzo M, Baryshnikova A, Myers CL, Andrews B, Boone C, (2011) Charting the genetic interaction map of a cell. Curr Opin Biotechnol 22: 66–74. doi: 10.1016/j.copbio.2010.11.001 21111604
60. Tong AH, Lesage G, Bader GD, Ding H, Xu H, et al. (2004) Global mapping of the yeast genetic interaction network. Science 303: 808–813. doi: 10.1126/science.1091317 14764870
61. Caydasi AK, Lohel M, Grunert G, Dittrich P, Pereira G, et al. (2012) A dynamical model of the spindle position checkpoint. Mol Syst Biol 8: 582. doi: 10.1038/msb.2012.15 22580890
62. Kim J, Luo G, Bahk YY, Song K, (2012) Cdc5-dependent asymmetric localization of bfa1 fine-tunes timely mitotic exit. PLoS Genet 8: e1002450. doi: 10.1371/journal.pgen.1002450 22253605
63. Konig C, Maekawa H, Schiebel E, (2010) Mutual regulation of cyclin-dependent kinase and the mitotic exit network. J Cell Biol 188: 351–368. doi: 10.1083/jcb.200911128 20123997
64. Cepeda-Garcia C, Delgehyr N, Juanes Ortiz MA, ten Hoopen R, Zhiteneva A, et al. (2010) Actin-mediated delivery of astral microtubules instructs Kar9p asymmetric loading to the bud-ward spindle pole. Mol Biol Cell 21: 2685–2695. doi: 10.1091/mbc.E10-03-0197 20534809
65. Juanes MA, Twyman H, Tunnacliffe E, Guo Z, ten Hoopen R, et al. (2013) Spindle pole body history intrinsically links pole identity with asymmetric fate in budding yeast. Curr Biol 23: 1310–1319. doi: 10.1016/j.cub.2013.05.057 23810537
66. Ahmadian MR, Stege P, Scheffzek K, Wittinghofer A, (1997) Confirmation of the arginine-finger hypothesis for the GAP-stimulated GTP-hydrolysis reaction of Ras. Nat Struct Biol 4: 686–689. doi: 10.1038/nsb0997-686 9302992
67. Scheffzek K, Ahmadian MR, Kabsch W, Wiesmuller L, Lautwein A, et al. (1997) The Ras-RasGAP complex: structural basis for GTPase activation and its loss in oncogenic Ras mutants. Science 277: 333–338. doi: 10.1126/science.277.5324.333 9219684
68. Gavriljuk K, Gazdag EM, Itzen A, Kotting C, Goody RS, et al. (2012) Catalytic mechanism of a mammalian Rab.RabGAP complex in atomic detail. Proc Natl Acad Sci U S A 109: 21348–21353. doi: 10.1073/pnas.1214431110 23236136
69. Langemeyer L, Nunes Bastos R, Cai Y, Itzen A, Reinisch KM, et al. (2014) Diversity and plasticity in Rab GTPase nucleotide release mechanism has consequences for Rab activation and inactivation. Elife 3: e01623. doi: 10.7554/eLife.01623 24520163
70. Geymonat M, Spanos A, Walker PA, Johnston LH, Sedgwick SG, (2003) In Vitro Regulation of Budding Yeast Bfa1/Bub2 GAP Activity by Cdc5. J Biol Chem 278: 14591–14594. doi: 10.1074/jbc.C300059200 12637549
71. Baro B, Rodriguez-Rodriguez JA, Calabria I, Hernaez ML, Gil C, et al. (2013) Dual Regulation of the mitotic exit network (MEN) by PP2A-Cdc55 phosphatase. PLoS Genet 9: e1003966. doi: 10.1371/journal.pgen.1003966 24339788
72. Johnson AE, Gould KL, (2011) Dma1 ubiquitinates the SIN scaffold, Sid4, to impede the mitotic localization of Plo1 kinase. EMBO J 30: 341–354. doi: 10.1038/emboj.2010.317 21131906
73. Krapp A, Cano E, Simanis V, (2003) Mitotic hyperphosphorylation of the fission yeast SIN scaffold protein cdc11p is regulated by the protein kinase cdc7p. Curr Biol 13: 168–172. doi: 10.1016/S0960-9822(02)01417-3 12546793
74. Singh NS, Shao N, McLean JR, Sevugan M, Ren L, et al. (2011) SIN-inhibitory phosphatase complex promotes Cdc11p dephosphorylation and propagates SIN asymmetry in fission yeast. Curr Biol 21: 1968–1978. doi: 10.1016/j.cub.2011.10.051 22119525
75. Feoktistova A, Morrell-Falvey J, Chen JS, Singh NS, Balasubramanian MK, et al. (2012) The fission yeast septation initiation network (SIN) kinase, Sid2, is required for SIN asymmetry and regulates the SIN scaffold, Cdc11. Mol Biol Cell 23: 1636–1645. doi: 10.1091/mbc.E11-09-0792 22419817
76. Hotz M, Barral Y, (2014) The Mitotic Exit Network: new turns on old pathways. Trends Cell Biol 24: 145–152. doi: 10.1016/j.tcb.2013.09.010 24594661
77. Hampoelz B, Knoblich JA, (2004) Heterotrimeric G proteins: new tricks for an old dog. Cell 119: 453–456. doi: 10.1016/j.cell.2004.10.025 15537535
78. Gonczy P, (2008) Mechanisms of asymmetric cell division: flies and worms pave the way. Nat Rev Mol Cell Biol 9: 355–366. doi: 10.1038/nrm2388 18431399
79. Wach A, Brachat A, Pohlmann R, Philippsen P, (1994) New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10: 1793–1808. doi: 10.1002/yea.320101310 7747518
80. Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, et al. (2004) A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes. Yeast 21: 947–962. doi: 10.1002/yea.1142 15334558
81. Sheff MA, Thorn KS, (2004) Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast 21: 661–670. doi: 10.1002/yea.1130 15197731
82. Fraschini R, Beretta A, Sironi L, Musacchio A, Lucchini G, et al. (2001) Bub3 interaction with Mad2, Mad3 and Cdc20 is mediated by WD40 repeats and does not require intact kinetochores. Embo J 20: 6648–6659. doi: 10.1093/emboj/20.23.6648 11726501
83. Surana U, Amon A, Dowzer C, McGrew J, Byers B, et al. (1993) Destruction of the CDC28/CLB mitotic kinase is not required for the metaphase to anaphase transition in budding yeast. Embo J 12: 1969–1978. 8491189
84. Fraschini R, Formenti E, Lucchini G, Piatti S, (1999) Budding yeast Bub2 is localized at spindle pole bodies and activates the mitotic checkpoint via a different pathway from Mad2. J Cell Biol 145: 979–991. doi: 10.1083/jcb.145.5.979 10352016