Proteome exploration to provide a resource for the investigation of Ganoderma lucidum

PLoS ONE

Volumn 10, Issue 3, 2015, Pages

  Yu, Guo Jun ^a   Yin, Ya Lin ^a   Yu, Wen Hui ^a   Liu, Wei ^a   Jin, Yan Xia ^a   Shrestha, Alok ^a   Yang, Qing ^a   Ye, Xiang Dong ^a   Sun, Hui ^a,b  

^a WUHAN UNIVERSITY   (China)

^b HUBEI UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA GALACTOSIDASE; ELONGATION FACTOR; FUNGAL PROTEIN; GLUCAN 1,3 BETA GLUCOSIDASE; GLUTATHIONE REDUCTASE; GLYCOSIDASE; PROTEOME; IMMUNOLOGIC FACTOR;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL DENSITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME DEGRADATION; GANODERMA LUCIDUM; GEL ELECTROPHORESIS; IMMUNOMODULATION; LYMPHOCYTE; MALE; MOUSE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DATABASE; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEOMICS; SIGNAL TRANSDUCTION; TANDEM MASS SPECTROMETRY; ANIMAL; C57BL MOUSE; CHEMISTRY; FRUITING BODY; GENE ONTOLOGY; METABOLISM; MOLECULAR GENETICS;

FUNGI; GANODERMA LUCIDUM;

AMINO ACID SEQUENCE; ANIMALS; FRUITING BODIES, FUNGAL; FUNGAL PROTEINS; GENE ONTOLOGY; IMMUNOLOGIC FACTORS; MALE; MICE, INBRED C57BL; MOLECULAR SEQUENCE ANNOTATION; MOLECULAR SEQUENCE DATA; PROTEOME; REISHI;

EID: 84924371087     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0119439     Document Type: Article

References (83)

1. Paterson RR. Ganoderma-a therapeutic fungal biofactory. Phytochemistry. 2006; 67(18):1985-2001. PMID: 16905165
2. Sanodiya BS, Thakur GS, Baghel RK, Prasad GB, Bisen PS. Ganoderma lucidum: a potent pharmacological macrofungus. Current pharmaceutical biotechnology. 2009; 10(8):717-42. PMID: 19939212
3. Boh B, Berovic M, Zhang J, Zhi-Bin L. Ganoderma lucidum and its pharmaceutically active compounds. Biotechnology annual review. 2007; 13:265-301. PMID: 17875480
4. Yoon HM, Jang KJ, Han MS, Jeong JW, Kim GY, Lee JH, et al. Ganoderma lucidum ethanol extract inhibits the inflammatory response by suppressing the NF-kappaB and toll-like receptor pathways in lipo-polysaccharide-stimulated BV2 microglial cells. Experimental and therapeutic medicine. 2013; 5 (3):957-63. PMID: 23408713
5. Wu GS, Guo JJ, Bao JL, Li XW, Chen XP, Lu JJ, et al. Anti-cancer properties of triterpenoids isolated from Ganoderma lucidum-a review. Expert opinion on investigational drugs. 2013; 22(8):981-92. doi: 10.1517/13543784.2013.805202 PMID: 23790022
6. Wang J, Wang Y, Liu X, Yuan Y, Yue T. Free radical scavenging and immunomodulatory activities of Ganoderma lucidum polysaccharides derivatives. Carbohydrate polymers. 2013; 91(1):33-8. doi: 10. 1016/j.carbpol.2012.08.017 PMID: 23044102
7. Zhou X, Lin J, Yin Y, Zhao J, Sun X, Tang K. Ganodermataceae: natural products and their related pharmacological functions. The American journal of Chinese medicine. 2007; 35(4):559-74. PMID: 17708623
8. Shiao MS. Natural products of the medicinal fungus Ganoderma lucidum: occurrence, biological activities, and pharmacological functions. Chemical record. 2003; 3(3):172-80. PMID: 12900937
9. Dudhgaonkar S, Thyagarajan A, Sliva D. Suppression of the inflammatory response by triterpenes isolated from the mushroom Ganoderma lucidum. International immunopharmacology. 2009; 9 (11):1272-80. doi: 10.1016/j.intimp.2009.07.011 PMID: 19651243
10. Kimura Y, Taniguchi M, Baba K. Antitumor and antimetastatic effects on liver of triterpenoid fractions of Ganoderma lucidum: mechanism of action and isolation of an active substance. Anticancer research. 2002; 22(6A):3309-18. PMID: 12530080
11. Wu X, Zeng J, Hu J, Liao Q, Zhou R, Zhang P, et al. Hepatoprotective effects of aqueous extract from Lingzhi or Reishi medicinal mushroom Ganoderma lucidum (higher basidiomycetes) on alpha-amani-tin-induced liver injury in mice. International journal of medicinal mushrooms. 2013; 15(4):383-91.PMID: 23796220
12. Li P, Deng YP, Wei XX, Xu JH. Triterpenoids from Ganoderma lucidum and their cytotoxic activities. Natural product research. 2013; 27(1):17-22. doi: 10.1080/14786419.2011.652961 PMID: 22263904
13. Bao X, Liu C, Fang J, Li X. Structural and immunological studies of a major polysaccharide from spores of Ganoderma lucidum (Fr.) Karst. Carbohydrate research. 2001; 332(1):67-74. PMID: 11403089
14. Zhang CR, Yang SP, Yue JM. Sterols and triterpenoids from the spores of Ganoderma lucidum. Natural product research. 2008; 22(13):1137-42. doi: 10.1080/14786410601129721 PMID: 18855213
15. Ko EM, Leem YE, Choi HT. Purification and characterization of laccase isozymes from the white-rot basidiomycete Ganoderma lucidum. Applied microbiology and biotechnology. 2001; 57(1-2):98-102.
16. D'Souza TM, Merritt CS, Reddy CA. Lignin-modifying enzymes of the white rot basidiomycete Ganoderma lucidum. Applied and environmental microbiology. 1999; 65(12):5307-13. PMID: 10583981
17. Shendure J, Ji H. Next-generation DNA sequencing. Nature biotechnology. 2008; 26(10):1135-45. doi: 10.1038/nbt1486 PMID: 18846087
18. Ansorge WJ. Next-generation DNA sequencing techniques. New biotechnology. 2009; 25(4):195-203. doi: 10.1016/j.nbt.2008.12.009 PMID: 19429539
19. Chen S, Xu J, Liu C, Zhu Y, Nelson DR, Zhou S, et al. Genome sequence of the model medicinal mushroom Ganoderma lucidum. Nature communications. 2012; 3:913. doi: 10.1038/ncomms1923 PMID: 22735441
20. Liu D, Gong J, Dai W, Kang X, Huang Z, Zhang HM, et al. The genome of Ganoderma lucidum provides insights into triterpenes biosynthesis and wood degradation [corrected]. PloS one. 2012; 7(5):e36146. doi: 10.1371/journal.pone.0036146 PMID: 22567134
21. Yu GJ, Wang M, Huang J, Yin YL, Chen YJ, Jiang S, et al. Deep insight into the Ganoderma lucidum by comprehensive analysis of its transcriptome. PloS one. 2012; 7(8):e44031. doi: 10.1371/journal.pone. 0044031 PMID: 22952861
22. Huang YH, Wu HY, Wu KM, Liu TT, Liou RF, Tsai SF, et al. Generation and analysis of the expressed sequence tags from the mycelium of Ganoderma lucidum. PloS one. 2013; 8(5):e61127. doi: 10.1371/ journal.pone.0061127 PMID: 23658685
23. Luo H, Sun C, Song J, Lan J, Li Y, Li X, et al. Generation and analysis of expressed sequence tags from a cDNA library of the fruiting body of Ganoderma lucidum. Chinese medicine. 2010; 5:9. doi: 10. 1186/1749-8546-5-9 PMID: 20230644
24. Ren A, Li MJ, Shi L, Mu DS, Jiang AL, Han Q, et al. Profiling and quantifying differential gene transcription provide insights into ganoderic acid biosynthesis in Ganoderma lucidum in response to methyl jasmonate. PloS one. 2013; 8(6):e65027. doi: 10.1371/journal.pone.0065027 PMID: 23762280
25. Park BH, Karpinets TV, Syed MH, Leuze MR, Uberbacher EC. CAZymes Analysis Toolkit (CAT): web service for searching and analyzing carbohydrate-active enzymes in a newly sequenced organism using CAZy database. Glycobiology. 2010; 20(12):1574-84. doi: 10.1093/glycob/cwq106 PMID: 20696711
26. Steen H, Mann M. The ABC's (and XYZ's) of peptide sequencing. Nature reviews Molecular cell biology. 2004; 5(9):699-711. PMID: 15340378
27. Sickmann A, Mreyen M, Meyer HE. Mass spectrometry-a key technology in proteome research. Advances in biochemical engineering/biotechnology. 2003; 83:141-76. PMID: 12934929
28. de Oliveira JM, de Graaff LH. Proteomics of industrial fungi: trends and insights for biotechnology. Applied microbiology and biotechnology. 2011; 89(2):225-37. doi: 10.1007/s00253-010-2900-0 PMID: 20922379
29. Monteiro R, Vitorino R, Domingues P, Radhouani H, Carvalho C, Poeta P, et al. Proteome of a methicil-lin-resistant Staphylococcus aureus clinical strain of sequence type ST398. Journal of proteomics. 2012; 75(10):2892-915. doi: 10.1016/j.jprot.2011.12.036 PMID: 22245554
30. Barros BH, da Silva SH, dos ReisMarques Edos R, Rosa JC, Yatsuda AP, Roberts DW, et al. A proteomic approach to identifying proteins differentially expressed in conidia and mycelium of the entomopathogenic fungus Metarhizium acridum. Fungal biology. 2010; 114(7):572-9. doi: 10.1016/j.funbio. 2010.04.007 PMID: 20943168
31. Scherl A, Francois P, Bento M, Deshusses JM, Charbonnier Y, Converset V, et al. Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth. Journal of microbiological methods. 2005; 60(2):247-57. PMID: 15590099
32. Matallana-Surget S, Derock J, Leroy B, Badri H, Deschoenmaeker F, Wattiez R. Proteome-wide analysis and diel proteomic profiling of the cyanobacterium Arthrospira platensis PCC 8005. PloS one. 2014; 9(6):e99076. doi: 10.1371/journal.pone.0099076 PMID: 24914774
33. D'Ambrosio C, Arena S, Rocco M, Verrillo F, Novi G, Viscosi V, et al. Proteomic analysis of apricot fruit during ripening. Journal of proteomics. 2013; 78:39-57. doi: 10.1016/j.jprot.2012.11.008 PMID: 23178875
34. Bharti RK, Srivastava S, Thakur IS. Proteomic analysis of carbon concentrating chemolithotrophic bacteria Serratia sp. for sequestration of carbon dioxide. PloS one. 2014; 9(3):e91300. doi: 10.1371/ journal.pone.0091300 PMID: 24619032
35. Horie K, Rakwal R, Hirano M, Shibato J, Nam HW, Kim YS, et al. Proteomics of two cultivated mushrooms Sparassis crispa and Hericium erinaceum provides insight into their numerous functional protein components and diversity. Journal of proteome research. 2008; 7(5):1819-35. doi: 10.1021/pr070369o PMID: 18380476
36. Johnson SL, Villarroel M, Rosengrave P, Carne A, Kleffmann T, Lokman PM, et al. Proteomic analysis of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid. PloS one. 2014; 9(8):e104155. doi: 10. 1371/journal.pone.0104155 PMID: 25089903
37. Reinhardt TA, Lippolis JD, Nonnecke BJ, Sacco RE. Bovine milk exosome proteome. Journal of proteomics. 2012; 75(5):1486-92. doi: 10.1016/j.jprot.2011.11.017 PMID: 22129587
38. Restelli L, Codrea MC, Savoini G, Ceciliani F, Bendixen E. LC-MS/MS analysis of visceral and subcutaneous adipose tissue proteomes in young goats with focus on innate immunity and inflammation related proteins. Journal of proteomics. 2014; 108:295-305. doi: 10.1016/j.jprot.2014.05.027 PMID: 24911890
39. Rahmad N, Al-Obaidi JR, Nor Rashid NM, Zean NB, Mohd Yusoff MH, Shaharuddin NS, et al. Comparative proteomic analysis of different developmental stages of the edible mushroom Termitomyces heimii. Biological research. 2014; 47(1):30. doi: 10.1186/0717-6287-47-30 PMID: 25053143
40. Yang J, Wang L, Ji X, Feng Y, Li X, Zou C, et al. Genomic and proteomic analyses of the fungus Arthrobotrys oligospora provide insights into nematode-trap formation. PLoS pathogens. 2011; 7(9): e1002179. doi: 10.1371/journal.ppat.1002179 PMID: 21909256
41. Wang M, Gu B, Huang J, Jiang S, Chen Y, Yin Y, et al. Transcriptome and proteome exploration to provide a resource for the study of Agrocybe aegerita. PloS one. 2013; 8(2):e56686. doi: 10.1371/journal. pone.0056686 PMID: 23418592
42. Yin Y, Yu G, Chen Y, Jiang S, Wang M, Jin Y, et al. Genome-wide transcriptome and proteome analysis on different developmental stages of Cordyceps militaris. PloS one. 2012; 7(12):e51853. doi: 10.1371/ journal.pone.0051853 PMID: 23251642
43. Hsu HC, Hsu CI, Lin RH, Kao CL, Lin JY. Fip-vvo, a new fungal immunomodulatory protein isolated from Volvariella volvacea. The Biochemical journal. 1997; 323 (Pt 2):557-65. PMID: 9163352
44. Hsin IL, Ou CC, Wu TC, Jan MS, Wu MF, Chiu LY, et al. GMI, an immunomodulatory protein from Ganoderma microsporum, induces autophagy in non-small cell lung cancer cells. Autophagy. 2011; 7 (8):873-82. PMID: 21490426
45. Lin CH, Hsiao YM, Ou CC, Lin YW, Chiu YL, Lue KH, et al. GMI, a Ganoderma immunomodulatory protein, down-regulates tumor necrosis factor alpha-induced expression of matrix metalloproteinase 9 via NF-kappaB pathway in human alveolar epithelial A549 cells. Journal of agricultural and food chemistry. 2010; 58(22):12014-21. doi: 10.1021/jf103068w PMID: 21028821
46. Kuan YC, Wu TJ, Kuo CY, Hsu JC, Chang WY, Sheu F. Molecular cloning of a new immunomodulatory protein from Anoectochilus formosanus which induces B cell IgM secretion through a T-independent mechanism. PloS one. 2011; 6(6):e21004. doi: 10.1371/journal.pone.0021004 PMID: 21698210
47. Jiang S, Chen Y, Wang M, Yin Y, Pan Y, Gu B, et al. A novel lectin from Agrocybe aegerita shows high binding selectivity for terminal N-acetylglucosamine. The Biochemical journal. 2012; 443(2):369-78. doi: 10.1042/BJ20112061 PMID: 22268569
48. Zhao C, Sun H, Tong X, Qi Y. An antitumour lectin from the edible mushroom Agrocybe aegerita. The Biochemical journal. 2003; 374(Pt 2):321-7. PMID: 12757412
49. Kino K, Yamashita A, Yamaoka K, Watanabe J, Tanaka S, Ko K, et al. Isolation and characterization of a new immunomodulatory protein, ling zhi-8 (LZ-8), from Ganoderma lucidium. The Journal of biological chemistry. 1989; 264(1):472-8. PMID: 2909532
50. Tanaka S, Ko K, Kino K, Tsuchiya K, Yamashita A, Murasugi A, et al. Complete amino acid sequence of an immunomodulatory protein, ling zhi-8 (LZ-8). An immunomodulator from a fungus, Ganoderma lucidium, having similarity to immunoglobulin variable regions. The Journal of biological chemistry. 1989; 264(28):16372-7. PMID: 2570780
51. Manavalan T, Manavalan A, Thangavelu KP, Heese K. Secretome analysis of Ganoderma lucidum cultivated in sugarcane bagasse. Journal of proteomics. 2012; 77:298-309. doi: 10.1016/j.jprot.2012.09. 004 PMID: 23000217
52. Wu Y, Wang D. A new class of natural glycopeptides with sugar moiety-dependent antioxidant activities derived from Ganoderma lucidum fruiting bodies. Journal of proteome research. 2009; 8(2):436-42. doi: 10.1021/pr800554w PMID: 18989955
53. Chen YY, Lin SY, Yeh YY, Hsiao HH, Wu CY, Chen ST, et al. A modified protein precipitation procedure for efficient removal of albumin from serum. Electrophoresis. 2005; 26(11):2117-27. PMID: 15880626
54. Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, et al. Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nature genetics. 2000; 25(1):25-9. PMID: 10802651
55. Tatusov RL, Fedorova ND, Jackson JD, Jacobs AR, Kiryutin B, Koonin EV, et al. The COG database: an updated version includes eukaryotes. BMC bioinformatics. 2003; 4:41. PMID: 12969510
56. Ishiyama M, Miyazono Y, Sasamoto K, Ohkura Y, Ueno K. A highly water-soluble disulfonated tetrazolium salt as a chromogenic indicator for NADH as well as cell viability. Talanta. 1997; 44(7):1299-305. PMID: 18966866
57. Lombard V, Golaconda Ramulu H, Drula E, Coutinho PM, Henrissat B. The carbohydrate-active enzymes database (CAZy) in 2013. Nucleic acids research. 2014; 42(Database issue):D490-5. doi: 10. 1093/nar/gkt1178 PMID: 24270786
58. Cantarel BL, Coutinho PM, Rancurel C, Bernard T, Lombard V, Henrissat B. The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics. Nucleic acids research. 2009; 37 (Database issue):D233-8. doi: 10.1093/nar/gkn663 PMID: 18838391
59. Levasseur A, Drula E, Lombard V, Coutinho PM, Henrissat B. Expansion of the enzymatic repertoire of the CAZy database to integrate auxiliary redox enzymes. Biotechnology for biofuels. 2013; 6(1):41. doi: 10.1186/1754-6834-6-41 PMID: 23514094
60. Aoshima M. Novel enzyme reactions related to the tricarboxylic acid cycle: phylogenetic/functional implications and biotechnological applications. Applied microbiology and biotechnology. 2007; 75 (2):249-55. PMID: 17333169
61. Nunes-Nesi A, Araujo WL, Obata T, Fernie AR. Regulation of the mitochondrial tricarboxylic acid cycle. Current opinion in plant biology. 2013; 16(3):335-43. doi: 10.1016/j.pbi.2013.01.004 PMID: 23462640
62. Terol J, Soler G, Talon M, Cercos M. The aconitate hydratase family from Citrus. BMC plant biology. 2010; 10:222. doi: 10.1186/1471-2229-10-222 PMID: 20958971
63. Aleksandrov A, Zvereva E, Field M. The mechanism of citryl-coenzyme A formation catalyzed by citrate synthase. The journal of physical chemistry B. 2014; 118(17):4505-13. doi: 10.1021/jp412346g PMID: 24720842
64. McNeil MB, Hampton HG, Hards KJ, Watson BN, Cook GM, Fineran PC. The succinate dehydrogenase assembly factor, SdhE, is required for the flavinylation and activation of fumarate reductase in bacteria. FEBS letters. 2014; 588(3):414-21. doi: 10.1016/j.febslet.2013.12.019 PMID: 24374335
65. Sanfelix-Haywood N, Coll-Marques JM, Yebra MJ. Role of alpha-phosphoglucomutase and phosphoglucose isomerase activities at the branching point between sugar catabolism and anabolism in Lactobacillus casei. Journal of applied microbiology. 2011; 111(2):433-42. doi: 10.1111/j.1365-2672.2011. 05045.x PMID: 21605291
66. Tomm JM, Krause C, Simon JC, Treudler R, von Bergen M, Averbeck M. Pyruvate kinase and phosphopyruvate hydratase as novel IgE reactive proteins in prawn. Journal of investigational allergology & clinical immunology. 2013; 23(6):443-5. doi: 10.1111/ddg.12103 PMID: 23721594
67. Zheng H, Beliavsky A, Tchigvintsev A, Brunzelle JS, Brown G, Flick R, et al. Structure and activity of the NAD(P)+-dependent succinate semialdehyde dehydrogenase YneI from Salmonella typhimurium. Proteins. 2013; 81(6):1031-41. doi: 10.1002/prot.24227 PMID: 23229889
68. Liebminger E, Veit C, Pabst M, Batoux M, Zipfel C, Altmann F, et al. Beta-N-acetylhexosaminidases HEXO1 and HEXO3 are responsible for the formation of paucimannosidic N-glycans in Arabidopsis thaliana. The Journal of biological chemistry. 2011; 286(12):10793-802. doi: 10.1074/jbc.M110. 178020 PMID: 21252225
69. Guranowski A, de Diego A, Sillero A, Sillero MAG. Uridine 5 '-polyphosphates (p(4)U and p(5)U) and uridine(5 ')polyphospho(5 ')nucleosides (Up(n)Ns) can be synthesized by UTP: glucose-1-phosphate uridylyltransferase from Saccharomyces cerevisiae. FEBS letters. 2004; 561(1-3):83-8. PMID: 15013780
70. Johswich A, Longuet C, Pawling J, Rahman AA, Ryczko M, Drucker DJ, et al. N-glycan remodeling on glucagon receptor is an effector of nutrient sensing by the hexosamine biosynthesis pathway. The Journal of biological chemistry. 2014; 289(23):15927-41. doi: 10.1074/jbc.M114.563734 PMID: 24742675
71. Ohnuma K, Hosono O, Dang NH, Morimoto C. Dipeptidyl peptidase in autoimmune pathophysiology. Advances in clinical chemistry. 2011; 53:51-84. PMID: 21404914
72. Horimoto Y, Dee DR, Yada RY. Multifunctional aspartic peptidase prosegments. New biotechnology. 2009; 25(5):318-24. doi: 10.1016/j.nbt.2009.03.010 PMID: 19491047
73. Liu DL, Yao DS, Liang YQ, Zhou TH, Song YP, Zhao L, et al. Production, purification, and characterization of an intracellular aflatoxin-detoxifizyme from Armillariella tabescens (E-20). Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association. 2001; 39(5):461-6.
74. Yao Z, Zhang C, Lu F, Bie X, Lu Z. Gene cloning, expression, and characterization of a novel acetaldehyde dehydrogenase from Issatchenkia terricola strain XJ-2. Applied microbiology and biotechnology. 2012; 93(5):1999-2009. doi: 10.1007/s00253-011-3541-7 PMID: 21858493
75. Lau SK, Chow WN, Wong AY, Yeung JM, Bao J, Zhang N, et al. Identification of microRNA-like RNAs in mycelial and yeast phases of the thermal dimorphic fungus Penicillium marneffei. PLoS neglected tropical diseases. 2013; 7(8):e2398. doi: 10.1371/journal.pntd.0002398 PMID: 23991243
76. Douglas CM. Fungal beta(1,3)-D-glucan synthesis. Medical mycology: official publication of the International Society for Human and Animal Mycology. 2001; 39 Suppl 1:55- 66.
77. Shahinian S, Bussey H. beta-1,6-Glucan synthesis in Saccharomyces cerevisiae. Molecular microbiology. 2000; 35(3):477-89. PMID: 10672173
78. Scott MG, Dullaghan E, Mookherjee N, Glavas N, Waldbrook M, Thompson A, et al. An anti-infective peptide that selectively modulates the innate immune response. Nature biotechnology. 2007; 25 (4):465-72. PMID: 17384586
79. Li S, Nie Y, Ding Y, Shi L, Tang X. Recombinant Expression of a Novel Fungal Immunomodulatory Protein with Human Tumor Cell Antiproliferative Activity from Nectria haematococca. International journal of molecular sciences. 2014; 15(10):17751-64. doi: 10.3390/ijms151017751 PMID: 25272229
80. Li F, Wen H, Liu X, Zhou F, Chen G. Gene cloning and recombinant expression of a novel fungal immunomodulatory protein from Trametes versicolor. Protein expression and purification. 2012; 82 (2):339-44. doi: 10.1016/j.pep.2012.01.015 PMID: 22342678
81. Ko JL, Hsu CI, Lin RH, Kao CL, Lin JY. A new fungal immunomodulatory protein, FIP-fve isolated from the edible mushroom, Flammulina velutipes and its complete amino acid sequence. European journal of biochemistry / FEBS. 1995; 228(2):244-9. PMID: 7705335
82. Jakucs E, Racz I, Lasztity D. Some characteristics and partial purification of the Ganoderma lucidum cellulase system. Acta microbiologica et immunologica Hungarica. 1994; 41(1):23-31. PMID: 7921848
83. Sripuan T, Aoki K, Yamamoto K, Tongkao D, Kumagai H. Purification and characterization of thermostable alpha-galactosidase from Ganoderma lucidum. Bioscience, biotechnology, and biochemistry. 2003; 67(7):1485-91. PMID: 12913291