Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris

Microbial Cell Factories

Volumn 14, Issue 1, 2015, Pages

  Krainer, Florian W ^a   Capone, Simona ^b   Jäger, Martin ^b   Vogl, Thomas ^a   Gerstmann, Michaela ^a   Glieder, Anton ^a   Herwig, Christoph ^b   Spadiut, Oliver ^b  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^b VIENNA UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

Apo protein; Cofactor; Heme; Heme biosynthesis; Horseradish peroxidase; Metabolic engineering; Pichia pastoris; Plant peroxidase; Recombinant protein production

Indexed keywords

AMINOLEVULINIC ACID; FUNGAL ENZYME; HEMIN; HEMOPROTEIN; HORSERADISH PEROXIDASE; RECOMBINANT ENZYME; RECOMBINANT HEME PEROXIDASE; UNCLASSIFIED DRUG; ALDEHYDE OXIDASE; FERROUS ION; FERROUS SULFATE; FUNGAL PROTEIN; HEME; RECOMBINANT PROTEIN; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVITY; ENZYME SYNTHESIS; GENE DOSAGE; GENE OVEREXPRESSION; GENE SEQUENCE; KOMAGATAELLA PASTORIS; METABOLIC ENGINEERING; NONHUMAN; PROTEIN TARGETING; REAL TIME POLYMERASE CHAIN REACTION; SINGLE NUCLEOTIDE POLYMORPHISM; SUPPLEMENTATION; BATCH CELL CULTURE; BIOSYNTHESIS; ENZYMOLOGY; GENETICS; METABOLISM; PICHIA; PROMOTER REGION;

ARMORACIA RUSTICANA; PICHIA PASTORIS;

ALDEHYDE OXIDASE; BATCH CELL CULTURE TECHNIQUES; FERROUS COMPOUNDS; FUNGAL PROTEINS; HEME; HORSERADISH PEROXIDASE; PICHIA; PLANT PROTEINS; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS;

EID: 84924085603     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-014-0187-z     Document Type: Article

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