Determination of a dynamic feeding strategy for recombinant Pichia pastoris strains

Methods in Molecular Biology

Volumn 1152, Issue , 2014, Pages 185-194

  Spadiut, Oliver ^a   Dietzsch, Christian ^a   Herwig, Christoph ^a  

^a NONE

Author keywords

Dynamic fed batch strategy; Methanol pulses; Pichia pastoris; Specific substrate uptake rate

Indexed keywords

ARTICLE; FED BATCH CULTURE; FED BATCH FERMENTATION; FEEDING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; KOMAGATAELLA PASTORIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; BATCH CELL CULTURE; BIOREACTOR; BIOSYNTHESIS; DRUG EFFECTS; GENETICS; GROWTH, DEVELOPMENT AND AGING; MICROBIOLOGY; PICHIA; PROCEDURES; SPECIES DIFFERENCE; TIME;

PICHIA PASTORIS;

METHANOL; RECOMBINANT PROTEIN;

BATCH CELL CULTURE TECHNIQUES; BIOREACTORS; METHANOL; PICHIA; RECOMBINANT PROTEINS; SPECIES SPECIFICITY; TIME FACTORS;

EID: 84921425098     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-0563-8_11     Document Type: Article

References (15)

1. Potgieter TI, Kersey SD, Mallem MR, Nylen AC, d’Anjou M (2010) Antibody expression kinetics in glycoengineered Pichia pastoris. Biotechnol Bioeng 106:918-927
2. Jacobs PP, Inan M, Festjens N, Haustraete J, Van Hecke A, Contreras R, Meagher MM, Callewaert N (2010) Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate. Microb Cell Fact 9:93
3. Sinha J, Plantz BA, Zhang W, Gouthro M, Schlegel V, Liu CP, Meagher MM (2003) Improved production of recombinant ovine interferon-tau by mut(+) strain of Pichia pastoris using an optimized methanol feed profile. Biotechnol Prog 19:794-802
4. Zhang W, Sinha J, Smith LA, Inan M, Meagher MM (2005) Maximization of production of secreted recombinant proteins in Pichia pastoris fed-batch fermentation. Biotechnol Prog 21:386-393
5. Ohya T, Ohyama M, Kobayashi K (2005) Optimization of human serum albumin production in methylotrophic yeast Pichia pastoris by repeated fed-batch fermentation. Biotechnol Bioeng 90:876-887
6. Khatri NK, Hoffmann F (2006) Oxygen-limited control of methanol uptake for improved production of a single-chain antibody fragment with recombinant Pichia pastoris. Appl Microbiol Biotechnol 72:492-498
7. Khatri NK, Hoffmann F (2006) Impact of methanol concentration on secreted protein production in oxygen-limited cultures of recombinant Pichia pastoris. Biotechnol Bioeng 93: 871-879
8. Cunha AE, Clemente JJ, Gomes R, Pinto F, Thomaz M, Miranda S, Pinto R, Moosmayer D, Donner P, Carrondo MJ (2004) Methanol induction optimization for scFv antibody fragment production in Pichia pastoris. Biotechnol Bioeng 86:458-467
9. d’Anjou MC, Daugulis AJ (2001) A rational approach to improving productivity in recombinant Pichia pastoris fermentation. Biotechnol Bioeng 72:1-11
10. Ren H, Yuan JQ, Bellgardt KH (2003) Macrokinetic model for methylotrophic Pichia pastoris based on stoichiometric balance. J Biotechnol 106:53-68
11. Ren HT, Yuan JQ (2005) Model-based specific growth rate control for Pichia pastoris to improve recombinant protein production. J Chem Technol Biotechnol 80:1268-1272
12. Dietzsch C, Spadiut O, Herwig C (2011) A dynamic method based on the specific substrate uptake rate to set up a feeding strategy for Pichia pastoris. Microb Cell Fact 10:14
13. Rodgers RC, Hill GE (1978) Equations for vapour pressure versus temperature: derivation and use of the Antoine equation on a hand-held programmable calculator. Br J Anaesth 50:415-424
14. Krainer FW, Dietzsch C, Hajek T, Herwig C, Spadiut O, Glieder A (2012) Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway. Microb Cell Fact 11:22
15. Dietzsch C, Spadiut O, Herwig C (2011) A fast approach to determine a fed batch feeding profile for recombinant Pichia pastoris strains. Microb Cell Fact 10:85