Multiplication of microbes below 0.690 water activity: Implications for terrestrial and extraterrestrial life

Environmental Microbiology

Volumn 17, Issue 2, 2015, Pages 257-277

  Stevenson, Andrew ^a   Burkhardt, Jürgen ^b   Cockell, Charles S ^c   Cray, Jonathan A ^a   Dijksterhuis, Jan ^d   Fox Powell, Mark ^c   Kee, Terence P ^e   Kminek, Gerhard ^f   Mcgenity, Terry J ^g   Timmis, Kenneth N ^h   Timson, David J ^a   Voytek, Mary A ⁱ   Westall, Frances ^j   Yakimov, Michail M ^k   Hallsworth, John E ^a  

^a QUEEN'S UNIVERSITY BELFAST   (United Kingdom)

^b UNIVERSITY OF BONN   (Germany)

^c UNIVERSITY OF EDINBURGH   (United Kingdom)

^d CBS KNAW FUNGAL BIODIVERSITY CENTRE   (Netherlands)

^e UNIVERSITY OF LEEDS   (United Kingdom)

^f EUROPEAN SPACE AGENCY   (Netherlands)

^g UNIVERSITY OF ESSEX   (United Kingdom)

^h TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

ⁱ NASA HEADQUARTERS   (United States)

^j CENTRE DE BIOPHYSIQUE MOLÉCULAIRE   (France)

^k CNR   (Italy)

more..

Author keywords

[No Author keywords available]

Indexed keywords

EUKARYOTA; PROKARYOTA; XEROMYCES BISPORUS;

SODIUM CHLORIDE; WATER;

ARCHAEON; ASCOMYCETES; ASTROBIOLOGY; BACTERIUM; CELL DIVISION; CYTOLOGY; ECOSYSTEM; MICROBIOLOGY; MICROCLIMATE; PHYSIOLOGY; PROKARYOTIC CELL; SALINITY;

ARCHAEA; ASCOMYCOTA; BACTERIA; CELL DIVISION; ECOSYSTEM; EXOBIOLOGY; EXTRATERRESTRIAL ENVIRONMENT; PROKARYOTIC CELLS; SALINITY; SODIUM CHLORIDE; WATER; WATER MICROBIOLOGY;

EID: 84923655103     PISSN: 14622912     EISSN: 14622920     Source Type: Journal    
DOI: 10.1111/1462-2920.12598     Document Type: Review

References (226)

1. Allwood, A.C., Walter, M.R., Burch, I.W., and Kamber, B.S. (2007) 3.43 billion-year-old stromatolite reef from the Pilbara Craton of Western Australia: ecosystem-scale insights to early life on Earth. Precambrian Res 158: 198-227.
2. Antón, J., Oren, A., Benlloch, S., Rodríguez-Valera, F., Amann, R., and Rosselló-Mora, R. (2002) Salinibacter ruber gen. nov., sp. nov., a novel, extremely halophilic member of the Bacteria from saltern crystallizer ponds. Int J Syst Evol Microbiol 52: 485-491.
3. Argyris, D., Tummala, N.R., Striolo, A., and Cole, D.R. (2008) Molecular structure and dynamics in thin water films at the silica and graphite surfaces. J Phys Chem C 112: 13587-13599.
4. Armolick, N., and Dickson, J.G. (1956) Minimum humidity requirement for germination of conidia of fungi associated with storage of grains. Phytopathology 46: 462-465.
5. Asada, S., Takano, M., and Shibasaki, I. (1979) Deoxyribonucleic acid strand breaks during drying of Escherichia coli on a hydrophobic filter membrane. Appl Environ Microbiol 37: 266-273.
6. Ayerst, G. (1969) The effects of moisture and temperature on growth and spore germination in some fungi. J Stored Prod Res 5: 127-141.
7. Baati, H., Guermazi, S., Amdouni, R., Gharsallah, N., Sghir, A., and Ammar, E. (2008) Prokaryotic diversity of a Tunisian multipond solar saltern. Extremophiles 12: 505-518.
8. Beaty, D.W., Buxbaum, K.L., Meyer, M.A., Barlow, N.G., Boynton, W.V., Clark, B.C., etal. (2006) Findings of the special regions science analysis group. Astrobiology 6: 677-732.
9. Bekker, M., Huinink, H.P., Adan, O.C.G., Samson, R.A., Wyatt, T., and Dijksterhuis, J. (2012) Production of an extracellular matrix as an isotropic growth phase of Penicillium rubens on gypsum. Appl Environ Microbiol 78: 6930-6937.
10. Bell, A.N.W., Magill, E., Hallsworth, J.E., and Timson, D.T. (2013) Effects of alcohols and compatible solutes on the activity of ß-galactosidase. Appl Biochem Biotechnol 169: 786-796.
11. Bhaganna, P., Volkers, R.J.M., Bell, A.N.W., Kluge, K., Timson, D.J., McGrath, J.W., etal. (2010) Hydrophobic substances induce water stress in microbial cells. Microb Biotechnol 3: 701-716.
12. Bing, H., and Ma, W. (2011) Laboratory investigation of the freezing point of saline soil. Cold Reg Sci Technol 67: 79-88.
13. Blake, R.E., Chang, S.J., and Lepland, A. (2010) Phosphate oxygen isotope evidence for a temperate and biologically active Archean ocean. Nature 464: 1029-1033.
14. Bootsma, A., Gillespie, T.J., and Sutton, J.C. (1973) Germination of Phyllosticta maydis conidia in an incubation chamber with control of high relative humidities. Phytopathology 63: 1157-1161.
15. Bradley, J.P., Ishii, H.A., Gillis-Davis, J.J., Ciston, J., Nielsen, M.H., Bechtel, H.A., etal. (2014) Detection of solar wind-produced water in irradiated rims on silicate minerals. Proc Natl Acad Sci USA 111: 1732-1735.
16. Brodie, H.J., and Neufeld, C.C. (1942) The development and structure of the conidia of Erysiphe polygoni DC and their germination at low humidity. Can J Res 20: 41-61.
17. Brown, A.D. (1976) Microbial water stress. Bacteriol Rev 40: 803-846.
18. Brown, A.D. (1990) Microbial Water Stress Physiology: Principles and Perspectives. Chichester, England: John Wiley & Sons.
19. Brown, R.H., Clark, R.N., Buratti, B.J., Cruickshank, D.P., Barnes, J.W., Mastrapa, R.M.E., etal. (2006) Composition and physical properties of Enceladus' surface. Science 10: 1425-1428.
20. Burch, A.Y., Finkel, O.M., Cho, J.K., Belkin, S., and Lindow, S.E. (2013) Diverse microhabitats experienced by Halomonas variabilis on salt-secreting leaves. Appl Environ Microbiol 79: 845-852.
21. Burch, A.Y., Zeisler, V., Yokota, K., Schreiber, L., and Lindow, S.E. (2014) The hygroscopic biosurfactant syringafactin produced by Pseudomonas syringae enhances fitness on leaf surfaces during fluctuating humidity. Environ Microbiol doi: 10.1111/1462-2920.12437. (in press).
22. Burkhardt, J., and Hunsche, M. (2013) 'Breath figures' on leaf surfaces - formation and effects of microscopic leaf wetness. Front Plant Sci 4: 422. doi: 10.3389/fpls.2013.00422.
23. Burns, B.P., Goh, F., Allen, M., and Neilan, B.A. (2004) Microbial diversity of extant stromatolites in the hypersaline marine environment of Shark Bay, Australia. Environ Microbiol 6: 1096-1101.
24. Campins, H., Hargrove, K., Pinilla-Alonso, N., Howell, E.S., Kelley, M.S., Licandro, J., etal. (2010) Water ice and organics on the surface of the asteroid 24 Themis. Nature 464: 1320-1321.
25. Carr, M. (2006) The Surface of Mars. Cambridge, UK: Cambridge University Press.
26. Carroll, J.E., and Wilcox, W.F. (2003) Effects of humidity on the development of grapevine powdery mildew. Phytopathology 93: 1137-1144.
27. Carter, J., Poulet, F., Bibring, J.P., Mangold, N., and Murchie, S. (2013) Hydrous minerals on Mars as seen by the CRISM and OMEGA imagined spectrometers: updated global view. J Geophys Res-Planet 118: 831-858.
28. Chang, W.S., van de Mortel, M., Nielsen, L., de Guzman, G.N., Li, X.H., and Halverson, L.J. (2007) Alginate production by Pseudomonas putida creates a hydrated microenvironment and contributes to biofilm architecture and stress tolerance under water-limiting conditions. J Bacteriol 189: 8290-8299.
29. Chin, J.P., Megaw, J., Magill, C.L., Nowotarski, K., Williams, J.P., Bhaganna, P., etal. (2010) Solutes determine the temperature windows for microbial survival and growth. Proc Natl Acad Sci USA 107: 7835-7840.
30. Chivian, D., Brodie, E.L., Alm, E.J., Culley, D.E., Dehal, P.S., DeSantis, T.Z., etal. (2008) Environmental genomics reveals a single-species ecosystem deep within Earth. Science 322: 275-278.
31. Christner, B.C., Morris, C.E., Foreman, C.M., Cai, R., and Sands, D.C. (2008) Ubiquity of biological ice nucleators in snowfall. Science 319: 1214.
32. Clancy, P., Brack, A., and Horneck, G. (2005) Looking for Life: Searching the Solar System. Cambridge, UK: Cambridge University Press.
33. Cobucci-Ponzano, B., Rossi, M., and Moracci, M. (2006) Interrupted genes in extremophilic Archaea: mechanisms of gene expression in early organisms. Orig Life Evol Biosph 36: 487-492.
34. Cockell, C.S., Brack, A., Wynn-Williams, D.D., Baglioni, P., Brandstätter, F., Demets, R., etal. (2007) Interplanetary transfer of photosynthesis: an experimental demonstration of a selective dispersal filter in planetary island biogeography. Astrobiology 7: 1-9.
35. Collins, M.A., and Buick, R.K. (1989) Effect of temperature on the spoilage of stored peas by Rhodotorula glutinis. Food Microbiol 6: 135-142.
36. Cray, J.A., Bell, A.N.W., Bhaganna, P., Mswaka, A.Y., Timson, D.J., and Hallsworth, J.E. (2013a) The biology of habitat dominance; can microbes behave as weeds? Microb Biotechnol 6: 453-492.
37. Cray, J.A., Russell, J.T., Timson, D.J., Singhal, R.S., and Hallsworth, J.E. (2013b) A universal measure of chaotropicity and kosmotropicity. Environ Microbiol 15: 287-296.
38. Da Silva, J.A.L., and Holm, N.G. (2014) Borophosphates and silicophosphates as plausible contributors to the emergence of life. J Colloid Interface Sci 431: 250-254.
39. Daffonchio, D., Borin, S., Brusa, T., Brusetti, L., van der Wielen, P.W.J.J., Bolhuis, H., etal. (2006) Stratified prokaryote network in the oxic-anoxic transition of a deep sea halocline. Nature 440: 203-207.
40. Daniel, R.M., Finney, J.L., and Stoneham, M. (2004) The molecular basis of life: is life possible without water? A discussion meeting held at the Royal Society, London, UK, 3-4 December 2003. Philos Trans R Soc Lond B Biol Sci 359: 1141-1328.
41. Dash, J.G., Rempel, A.W., and Wettlaufer, J.S. (2006) The physics of premelted ice and its geophysical consequences. Rev Mod Phys 78: 698-741.
42. Derjaguin, B.V., and Churaev, N.V. (1986) Properties of water layers adjacent to interfaces. In Fluid Interfacial Phenomena. Croxton, C.A. (ed.). New York, NY, USA: John Wiley & Sons, pp. 663-738.
43. D'Hondt, S., Rutherford, S., and Spivack, A.J. (2002) Metabolic activity of subsurface life in deep-sea sediments. Science 295: 2067-2070.
44. Dickson, J.L., Head, J.W., Levy, J.S., and Marchant, D.R. (2013) Don Juan Pond, Antarctica: near-surface CaCl2-brine feeding Earth's most saline lake and implications for Mars. Sci Rep 3: 1166.
45. Doroshenko, E.A., Zenova, G.M., Zvyagintsev, D.G., and Sudnitsyn, I.I. (2005) Spore germination and mycelial growth of streptomycetes at different humidity levels. Mikrobiologiia 74: 690-694.
46. Doroshenko, E.A., Zenova, G.M., Sudnicin, I.I., and Zvyagintsev, D.G. (2006) Influence of humidity on soil mycelial bacteria. Vestn Mosk U Poch 1: 45-48.
47. Drewello, R., and Weissmann, R. (1997) Microbially influenced corrosion of glass. Appl Microbiol Biotechnol 47: 337-346.
48. Duda, V.I., Danilevich, V.N., Suzina, N.E., Shorokhova, A.P., Dmitriev, V.V., Mokhova, O.N., and Akimov, V.N. (2004) Changes in the fine structure of microbial cells induced by chaotropic salts. Mikrobiologiya 73: 341-349.
49. Duda, V.I., Danilevich, V.N., Akimov, V.N., Suzina, N.E., Dmitriev, V.V., and Shorokhova, A.P. (2005) Fluorescence microscopic study of microorganisms treated with chaotropic agents. Mikrobiologiya 74: 434-439.
50. Dundas, I. (1998) Was the environment for primordial life hypersaline? Extremophiles 2: 375-377.
51. Dunn, R.V., and Daniel, R.M. (2004) The use of gas-phase substrates to study enzyme catalysis at low hydration. Philos Trans R Soc Lond B Biol Sci 359: 1309-1320.
52. Elevi Bardavid, R., Khristo, P., and Oren, A. (2008) Interrelationships between Dunaliella and halophilic prokaryotes in saltern crystallizer ponds. Extremophiles 12: 5-14.
53. Fahlgren, C., Hagström, A., Nilsson, D., and Zweifel, U.L. (2010) Annual variations in the diversity, viability, and origin of airborne bacteria. Appl Environ Microbiol 76: 3015-3025.
54. Fakes, M.G., Dali, M.V., Haby, T.A., Morris, K.R., Varia, S.A., and Serajuddin, A.T.M. (2000) Moisture sorption behavior of selected bulking agents used in lyophilized products. PDA J Pharm Sci Technol 54: 144-149.
55. Falk, M., Hartman, K.A., and Lord, R.C. (1963) Hydration of deoxyribonucleic acid. II. An infrared study. J Am Chem Soc 85: 387-391.
56. Ferro Fontán, C., and Chirife, J. (1981) The evaluation of water activity in aqueous-solutions from freezing-point depression. J Food Technol 16: 21-30.
57. Fischer, E., Martínez, G.M., Elliott, H.M., and Rennó, N.O. (2014) Experimental evidence for the formation of liquid saline water on Mars. Geophys Res Lett 41: 4456-4462.
58. Foucher, F., Westall, F., Brandstatter, F., Demets, R., Parnell, J., Cockell, C.S., etal. (2010) Testing the survival of microfossils in artificial Martian sedimentary meteorites during entry into Earth's atmosphere: the STONE 6 experiment. Icarus 207: 616-630.
59. Franck, S., von Bloh, W., and Bounama, C. (2007) Maximum number of habitable planets at the time of Earth's origin: new hints for panspermia and the mediocrity principle. Int J Astrobiol 6: 153-157.
60. Frank, M., and Hess, E. (1941) Studies on salt fish: V. Studies on Sporendonema epizoum from 'Dun' salt fish. Can J Fish Aquat Sci 5b: 276-286.
61. Fries, M.D., Steele, A., and Zolensky, M. (2012) Halogen-substituted methane in Monahans halite. Meteorit Planet Sci Supplement 75: 5381.
62. Garcia-Pichel, F., and Pringault, O. (2001) Cyanobacteria track water in desert soil. Nature 413: 380-381.
63. Garvie, L.A., Knauth, L.P., Bungartz, F., Klonowski, S., and Nash, T.H., 3rd (2008) Life in extreme environments: survival strategy of the endolithic desert lichen Verrucaria rubrocincta. Naturwissenschaften 95: 705-712.
64. de Goffau, M.C., van Dijl, J.M., and Harmsen, H.J.M. (2011) Microbial growth on the edge of desiccation. Environ Microbiol 13: 2328-2335.
65. Gramain, A., Díaz, G.C., Demergasso, C., Lowenstein, T.K., and McGenity, T.J. (2011) Archaeal diversity along a subterranean salt core from the Salar Grande (Chile). Environ Microbiol 13: 2105-2121.
66. Grant, W.D. (2004) Life at low water activity. Philos Trans R Soc Lond B Biol Sci 359: 1249-1266.
67. Greenspan, L. (1977) Humidity fixed points of binary saturated aqueous solutions. J Res Nat Bur Stand-A Phys Chem 81A: 89-96.
68. Hallsworth, J.E. (1998a) Ethanol-induced water stress in yeast. J Ferment Bioeng 85: 125-137.
69. Hallsworth, J.E., and Magan, N. (1994) Effects of KCl concentration on accumulation of acyclic sugar alcohols and trehalose in conidia of three entomopathogenic fungi. Lett Appl Microbiol 18: 8-11.
70. Hallsworth, J.E., and Magan, N. (1995) Manipulation of intracellular glycerol and erythritol enhances germination of conidia at low water availability. Microbiol-SGM 29: 7-13.
71. Hallsworth, J.E., and Nomura, Y. (1999) A simple method to determine the water activity of ethanol-containing samples. Biotechnol Bioeng 62: 242-245.
72. Hallsworth, J.E., Nomura, Y., and Iwahara, M. (1998b) Ethanol-induced water stress and fungal growth. J Ferment Bioeng 86: 451-456.
73. Hallsworth, J.E., Heim, S., and Timmis, K.N. (2003a) Chaotropic solutes cause water stress in Pseudomonas putida. Environ Microbiol 5: 1270-1280.
74. Hallsworth, J.E., Prior, B.A., Nomura, Y., Iwahara, M., and Timmis, K.N. (2003b) Compatible solutes protect against chaotrope (ethanol)-induced, nonosmotic water stress. Appl Environ Microbiol 69: 7032-7034.
75. 2-containing environments: chaotropicity defines the window. Environ Microbiol 9: 803-813.
76. Harris, R.F. (1981) The effect of water potential on microbial growth and activity. In Water Potential Relations in Soil Microbiology. Parr, J.F., etal. (eds). Madison, WI, USA: Soil Science Society of America, pp. 23-95.
77. Harrison, J.P., Gheeraert, N., Tsigelnitskiy, D., and Cockell, C.S. (2013) The limits for life under multiple extremes. Trends Microbiol 21: 204-212.
78. Hocking, A.D. (2003) Microbiological facts and fictions in grain storage. In Stored Grain in Australia. Wright, E.J., Webb, M.C., and Highley, E. (eds). Canberra, Australia: CSIRO, pp. 55-58. Proceedings of the Australian Postharvest Technical Conference.
79. Hopkins, B., Elberling, B., Greenfield, L.G., Gregorich, E.G., Novis, P., O'Donnell, A.G., and Sparrow, A.D. (2005) Soil microorganisms in Antarctic dry valleys: resource supply and utilization. In Micro-organisms and Earth Systems-Advances in Geomicrobiology. Gadd, G., Semple, K., and Lappin-Scott, H. (eds). Cambridge, UK: Cambridge University Press, pp. 71-84.
80. Hu, Q., and Wang, J.S.Y. (2003) Aqueous-phase diffusion in unsaturated geologic media: a review. Crit Rev Environ Sci Technol 33: 275-297.
81. Jaenicke, R., and Bohm, G. (1998) The stability of proteins in extreme environments. Curr Opin Struct Biol 8: 738-748.
82. Javor, B.J. (1984) Growth potential of halophilic bacteria isolated from solar salt environments: carbon sources and salt requirements. Appl Environ Microbiol 48: 352-360.
83. Jepsen, S.M., Priscu, J.C., Grimm, R.E., and Bullock, M.A. (2007) The potential for lithoautotrophic life on Mars: application to shallow interfacial water environments. Astrobiology 7: 342-354.
84. Kane, D.A., and Williamson, K.J. (1983) Bacterial toxicity and metabolism of hydrazine fuels. Arch Environ Contam Toxicol 12: 447-453.
85. Kashangura, C., Hallsworth, J.E., and Mswaka, A.Y. (2006) Phenotypic diversity amongst strains of Pleurotus sajor-caju: implications for cultivation in arid environments. Mycol Res 110: 312-317.
86. Khemakhem, H., Elloumi, J., Moussa, M., Aleya, L., and Ayadi, H. (2010) The concept of ecological succession applied to phytoplankton over four consecutive years in five ponds featuring a salinity gradient. Estuar Coast Shelf Sci 88: 33-44.
87. Kminek, G., Rummel, J.D., Cockell, C.S., Atlas, R., Barlow, N., Beaty, D., etal. (2010) Report of the COSPAR Mars special regions colloquium. Adv Space Res 46: 811-829.
88. Kminek, G., Conley, C., Allen, C.C., Bartlett, D.H., Beaty, D.W., Benning, D.G., etal. (2014) Report of the workshop for life detection on samples from Mars. Life Sci Space Res 2: 1-5.
89. Knauth, L.P., and Lowe, D.R. (2003) High Archean climatic temperature inferred from oxygen isotope geochemistry of cherts in the 3.5Ga Swaziland Supergroup, South Africa. Geol Soc Am Bull 115: 566-580.
90. Kreuzer-Martin, H.W., Ehrlinger, J.R., and Hegg, E.L. (2005) Oxygen isotopes indicate most intracellular water in log-phase Escherichia coli is derived from metabolism. Proc Natl Acad Sci USA 102: 17337-17341.
91. Kreuzer-Martin, H.W., Lott, M.J., Ehleringer, J.R., and Hegg, E.L. (2006) Metabolic processes account for the majority of the intracellular water in log-phase Escherichia coli cells as revealed by hydrogen isotopes. Biochemistry 45: 13622-13630.
92. Kurkal, V., Daniel, R.M., Finney, J.L., Tehei, M., Dunn, R.V., and Smith, J.C. (2005) Enzyme activity and flexibility at very low hydration. Biophys J 89: 1282-1287.
93. Küppers, M., O'Rourke, L., Bockelée-Morvan, D., Zakharov, V., Lee, S., von Allmen, P., etal. (2014) Localized sources of water vapour on the dwarf planet (1) Ceres. Nature 505: 525-527.
94. Labuza, T.P., Acott, K., Tatini, S.R., Lee, R.Y., Flink, J., and McCall, W. (1976) Water activity determination - collaborative study of different methods. J Food Sci 41: 910-917.
95. Lange, O.L., Kilian, E., and Ziegler, H. (1986) Water vapour uptake and photosynthesis of lichens: performance differences in species with green and blue-green algae as phycobionts. Oecologia 71: 104-110.
96. Lange, O.L., Meyer, A., Zellner, H., and Heber, U. (1994) Photosynthesis and water relations of lichen soil crusts: field measurements in the coastal fog zone of the Namib Desert. Funct Ecol 8: 253-264.
97. 2 exchange of the terrestrial lichen Teloschistes capensis in the Namib fog desert: measurements during two seasons in the field and under controlled conditions. Flora 201: 268-280.
98. Leong, S.L.L., Lantz, H., Pettersson, O.V., Frisvad, J.C., Thrane, U., Heipieper, H.J., etal. (2014) Genome and physiology of the ascomycete filamentous fungus Xeromyces bisporus, the most xerophilic organism isolated to date. Environ Microbiol doi: 10.1111/1462-2920.12596. (in press)
99. Lievens, B., Hallsworth, J.E., Belgacem, Z.B., Pozo, M.I., Stevenson, A., Willems, K.A., and Jacquemyn, H. (2014) Microbiology of sugar-rich environments: diversity, ecology, and system constraints. Environ Microbiol doi:10.1111/1462-2920.12570. (in press).
100. Lomstein, B.A., Langerhuus, A.T., D'Hondt, S., Jorgensen, B.B., and Spivack, A.J. (2012) Endospore abundance, microbial growth and necromass turnover in deep sub-seafloor sediment. Nature 484: 101-104.
101. Longo, L.M., and Blaber, M. (2014) Prebiotic protein design supports a halophile origin of foldable proteins. Front Microbiol 4: 418.
102. Longo, L.M., Lee, J., and Blaber, M. (2013) Simplified protein design biased for prebiotic amino acids yields a foldable, halophilic protein. Proc Natl Acad Sci USA 110: 2135-2139.
103. Lopez, M., Kurkal-Siebert, V., Dunn, R.V., Tehei, M., Finney, J.L., Smith, J.C., etal. (2010) Activity and dynamics of an enzyme, pig liver esterase, in near-anhydrous conditions. Biophys J 99: L62-L64.
104. Lowe, D.R., and Fisher-Worrell, G. (1999) Sedimentology, mineralogy, and implications of silicified evaporites in the Kromberg Formation, Barberton greenstone belt, South Africa. Geol S Am S 329: 167-188.
105. Lowenstein, T.K., Schubert, B.A., and Timofeeff, M.N. (2011) Microbial communities in fluid inclusions and long-term survival in halite. GSA Today 21: 4-9.
106. McCammick, E.M., Gomase, V.S., Timson, D.J., McGenity, T.J., and Hallsworth, J.E. (2010) Water-hydrophobic compound interactions with the microbial cell. In Handbook of Hydrocarbon and Lipid Microbiology - Hydrocarbons, Oils and Lipids: Diversity, Properties and Formation, Vol. 2. Timmis, K.N. (ed.). New York, NY, USA: Springer, pp. 1451-1466.
107. McEwen, A.S., Dundas, C.M., Mattson, S.S., Toigo, A.D., Ojha, L., Wray, J.J., etal. (2014) Recurring slope lineae in equatorial regions of Mars. Nature Geosci 7: 53-58.
108. McGenity, T.J., Gemmell, R.T., Grant, W.D., and Stan-Lotter, H. (2000) Origins of halophilic microorganisms in ancient salt deposits. Environ Microbiol 2: 243-250.
109. Manners, J.G., and Hossain, S.M.M. (1963) Effects of temperature and humidity on conidial germination in Erysiphe graminis. T Brit Mycol Soc 46: 225-234.
110. Manzoni, S., Schimel, J.P., and Porporato, A. (2012) Responses of soil microbial communities to water-stress: results from a meta-analysis. Ecology 93: 930-938.
111. 12 and its exobiological significance. Planet Space Sci 50: 693-709.
112. Marion, G.M. (1997) A theoretical evaluation of mineral stability in Don Juan Pond, Wright Valley, Victoria Land. Antarct Sci 9: 92-99.
113. Marion, G.M., and Kargel, J.S. (2008) Cold Aqueous Planetary Geochemistry with FREZCHEM: From Modeling to the Search for Life at the Limits. Berlin, Germany: Springer-Verlag.
114. Marion, G.M., Fritsen, C.H., Eicken, H., and Payne, M.C. (2003) The search for life on Europa: limiting environmental factors, potential habitats, and Earth analogues. Astrobiology 3: 785-811.
115. Marion, G.M., Kargen, J.S., Catling, D.C., and Jakubowski, S.D. (2005) Effects of pressure on aqueous chemical equilibria at subzero temperatures with applications to Europa. Geochim Cosmochim Acta 69: 259-274.
116. Martínez, G.M., and Renno, N.O. (2013) Water and brines on Mars: current evidence and implications for MSL. Space Sci Rev 175: 29-51.
117. Matson, D.L., Castillo, J.C., and Lunine, J. (2007) Enceladus' plume: compositional evidence for a hot interior. Icarus 187: 569-573.
118. Mauer, L.J., and Taylor, L.S. (2010) Water-solids interactions: deliquescence. Annu Rev Food Sci Technol 1: 41-63.
119. Miller, E.J., and Chibnall, A.C. (1932) The proteins of grasses. Biochem J 26: 392-402.
120. Mormile, M.R., Hong, B.Y., and Benison, K.C. (2009) Molecular analysis of the microbial communities of Mars analog lakes in Western Australia. Astrobiology 9: 919-930.
121. Morris, C.E., Conen, F., Alex Huffman, J., Phillips, V., Pöschl, U., and Sands, D.C. (2014) Bioprecipitation: a feedback cycle linking Earth history, ecosystem dynamics and land use through biological ice nucleators in the atmosphere. Glob Chang Biol 20: 341-351.
122. Moyano, F.E., Manzoni, S., and Chenu, C. (2013) Responses of soil heterotrophic respiration to moisture availability: an exploration of processes and models. Soil Biol Biochem 59: 72-85.
123. Möhlmann, D. (2005) Adsorption water-related potential chemical and biological processes in the upper Martian surface. Astrobiology 5: 770-777.
124. Möhlmann, D. (2009) Are nanometric films of liquid undercooled interfacial water bio-relevant? Cryobiology 58: 256-261.
125. Möhlmann, D. (2011) Three types of liquid water in icy surfaces of celestial bodies. Planet Space Sci 59: 1082-1086.
126. Möhlmann, D. (2012) Widen the belt of habitability! Orig Life Evol Biosph 42: 93-100.
127. Möhlmann, D.T.F. (2004) Water in the upper Martian surface at mid- and low-latitudes: presence, state, and consequences. Icarus 168: 318-323.
128. Möhlmann, D.T.F. (2008) The influence of van der Waals forces on the state of water in the shallow subsurface of Mars. Icarus 195: 131-139.
129. Mullakhanbhai, M.F., and Larsen, H. (1975) Halobacterium volcanii spec. nov., a Dead Sea halobacterium with a moderate salt requirement. Arch Microbiol 104: 207-214.
130. Muñoz-Iglesias, V., Bonales, L.J., and Prieto-Ballesteros, O. (2013) pH and salinity evolution of Europa's brines: Raman spectroscopy study of fractional precipitation at 1 and 300 bar. Astrobiology 13: 693-702.
131. Mykytczuk, N.C.S., Foote, S.J., Omelon, C.R., Southam, G., Greer, C.W., and Whyte, L.G. (2013) Bacterial growth at -15C; molecular insights from the permafrost bacterium Planococcus halocryophilus Or1. ISME J 7: 1211-1226.
132. Nagel, F.J.J.I., Tramper, J., Bakker, M.S.N., and Rinzema, A. (2001) Model for on-line moisture-content control during solid-state fermentation. Biotechnol Bioeng 76: 291-302.
133. Nimmo, F., Spencer, J.R., Pappalardo, R.T., and Mullen, M.E. (2007) Shear heating as the origin of the plumes and heat flux on Enceladus. Nature 447: 289-291.
134. Oren, A. (1988) The microbial ecology of the Dead Sea. In Advances in Microbial Ecology. Marshall, K.C. (ed.). New York, NY, USA: Plenum Press, pp. 193-229.
135. Oren, A. (1992) Ecology of extremely halophilic bacteria. In The Biology of Halophilic Bacteria. Vreeland, R.H., and Hochstein, L.I. (eds). Boca Raton, FL, USA: CRC Press, pp. 25-54.
136. Oren, A. (1993) The Dead Sea-alive again. Experientia 49: 518-522.
137. Oren, A. (1999) Bioenergetic aspects of halophilism. Microbiol Mol Biol Rev 63: 334-348.
138. Oren, A. (2002) Diversity of halophilic microorganisms: environments, phylogeny, physiology, and applications. J Ind Microbiol Biotechnol 28: 56-63.
139. Oren, A. (2010) The dying Dead Sea: the microbiology of an increasingly extreme environment. Lakes Reser: Res Manag 15: 215-222.
140. Oren, A. (2011) Thermodynamic limits to microbial life at high salt concentrations. Environ Microbiol 13: 1908-1923.
141. Oren, A. (2013) Life in magnesium- and calcium-rich hypersaline environments: salt stress by chaotropic ions. In Polyextremophiles: Life Under Multiple Forms of Stress. Cellular Origin, Life in Extreme Habitats and Astrobiology. Seckbach, J., Oren, A., and Stan-Lotter, H. (eds). Dordrecht, the Netherlands: Springer Science and Business Media, pp. 215-232.
142. Oren, A., and Hallsworth, J.E. (2014) Microbial weeds in hypersaline habitats: the enigma of the weed-like Haloferax mediterranei. FEMS Microbiol Lett. (in press).
143. Oriol, E., Raimbault, M., Roussos, S., and Viniegra-Gonzales, G. (1988) Water and water activity in the solid state fermentation of cassava starch by Aspergillus niger. Appl Microbiol Biotechnol 27: 498-503.
144. Papendick, R.I., and Campbell, G.S. (1981) Theory and measurement of water potential. In Water Potential Relationships in Soil Microbiology. Parr, J.F., etal. (eds). Madison, WI, USA: Soil Science Society of America Publications, pp. 1-22.
145. Parkes, R.J., Cragg, B.A., and Wellsbury, P. (2000) Recent studies on bacterial populations and processes in subseafloor sediments: a review. Hydrogeol J 8: 11-28.
146. Parkinson, C.D., Liang, M.-C., Yung, Y.L., and Kirshcivnk, J.L. (2008) Habitability of Enceladus: planetary conditions for life. Orig Life Evol Biosph 38: 355-369.
147. Pasanen, A.L., Pasanen, P., Jantunen, M.J., and Kalliokoski, P. (1991) Significance of air humidity and air velocity for fungal spore release into the air. Atmos Environ 25: 459-462.
148. Pavlov, A.K., Shelegedina, V.N., Vdovina, M.A., and Pavlov, A.A. (2010) Growth of microorganisms in Martian-like shallow subsurface conditions: laboratory modeling. Int J Astrobiol 9: 51-58.
149. Pearson, R.T., and Derbyshire, W. (1974) NMR studies of water adsorbed on a number of silica surfaces. J Colloid Interf Sci 46: 232-248.
150. Peterson, C.A., and Cowling, E.B. (1973) Influence of various initial moisture contents on decay of sitka spruce and sweetgum sapwood by Polyporus versicolor in the soil-block test. Phytopathology 63: 235-237.
151. Pintado, A., and Sancho, L.G. (2002) Ecological significance of net photosynthesis activation by water vapour uptake in Ramalina capitatafrom rain-protected habitats in central Spain. Lichenologist 34: 403-413.
152. Pitt, J.I. (1975) Xerophilic fungi and the spoilage of foods of plant origin. In Water Relations of Foods. Duckworth, R.B. (ed.). London, UK: Academic Press, pp. 273-307.
153. Pitt, J.I., and Christian, J.H.B. (1968) Water relations of xerophilic fungi isolated from prunes. Appl Environ Microbiol 16: 1853-1858.
154. Postberg, F., Kempf, S., Schmidt, J., Brillantov, N., Beinsen, A., Abel, B., etal. (2009) Sodium salts in E-ring ice grains from an ocean below the surface of Enceladus. Nature 459: 1098-1101.
155. Postberg, F., Schmidt, J., Hillier, J., Kempf, S., and Srama, R. (2011) A salt-water reservoir as the source compositionally stratified plume on Enceladus. Nature 474: 620-622.
156. Potts, M. (1994) Desiccation tolerance of prokaryotes. Microbiol Rev 58: 755-805.
157. Quesada, E., Bejar, V., Valderrama, M.J., and Ramos-Cormenzana, A. (1987) Growth characteristics and salt requirement of Deleya halophila in a defined medium. Curr Microbiol 16: 21-25.
158. Qvit-Raz, N., Jurkevitch, E., and Belkin, S. (2008) Drop-size soda lakes: transient microbial habitats on a salt-secreting desert tree. Genetics 178: 1615-1622.
159. Rajeev, L., Da Rocha, U.N., Klitgord, N., Luning, E.G., Fortney, J., Axen, S.D., etal. (2013) Dynamic cyanobacterial response to hydration and dehydration in a desert biological soil crust. ISME J 7: 2178-2191.
160. Ramos-Cormenzana, A. (1992) Ecology of moderately halophilic bacteria. In The Biology of Halophilic Bacteria. Vreeland, R.H., and Hochstein, L.I. (eds). Boca Raton, FL, USA: CRC Press, pp. 55-86.
161. Rampino, M.R., and Caldeira, K. (1994) The goldilocks problem: climate evolution and long-term habitability of terrestrial planets. Annu Rev Astron Astrophys 32: 83-114.
162. Ratkowsky, D.A., Lowry, R.K., McMeekin, T.A., Stokes, A.N., and Chandler, R.E. (1983) Model for bacterial culture growth rate throughout the entire biokinetic temperature range. J Bacteriol 154: 1222-1226.
163. Raulin, F. (2008) Astrobiology and habitability of Titan. Space Sci Rev 135: 37-48.
164. Raviv, U., Laurat, P., and Klein, J. (2001) Fluidity of water confined to subnanometre films. Nature 413: 51-54.
165. Reponen, T., Willeke, K., Ulevicius, V., Reponen, A., and Grinshpun, S.A. (1996) Effect of relative humidity on the aerodynamic diameter and respiratory deposition of fungal spores. Atmos Environ 30: 3967-3974.
166. Rivkina, E., Friedmann, E., McKay, C., and Gilichinsky, D. (2000) Metabolic activity of permafrost bacteria below the freezing point. Appl Environ Microbiol 66: 3230-3233.
167. Roa, V., and Tapia, M.S. (1998) Estimating water activity in systems containing multiples solutes based on solute properties. J Food Sci 63: 559-564.
168. Robinson, J.L., Pyzyna, B., Atrasz, R.G., Henderson, C.A., Morrill, K.L., Burd, A.M., etal. (2005) Growth kinetics of extremely halophilic Archaea (family Halobacteriaceae) as revealed by Arrhenius plots. J Bacteriol 187: 923-929.
169. Rodriguez-Valera, F. (1992) Introduction to saline environments. In The Biology of Halophilic Bacteria. Vreeland, R.H., and Hochstein, L.I. (eds). Boca Raton, FL, USA: CRC Press, pp. 1-24.
170. de Ronde, C.E.J., Channer, D.M.R., Faure, K., Bray, C.J., and Spooner, E.T.C. (1997) Fluid chemistry of Archean seafloor hydrothermal vents; implications for the composition of circa 3.2Ga seawater. Geochim Cosmochim Acta 61: 4025-4042.
171. Rothschild, L.J., and Mancinelli, R.L. (2001) Life in extreme environments. Nature 409: 1092-1101.
172. Rothschild, L.J., Giver, L.J., White, M.R., and Mancinelli, R.L. (1994) Metabolic activity of microorganisms in evaporates. J Phycol 30: 431-438.
173. Rubin, A.E., Zolensky, M.E., and Bodnar, R.J. (2002) The halite-bearing Zag and Monahans (1998) meteorite breccias: shock metamorphism, thermal metamorphism and aqueous alteration on the H-chondrite parent body. Meteorit Planet Sci 37: 125-141.
174. Samarkin, V.A., Madigan, M.T., Bowles, M.W., Casciotti, K.L., Priscu, J.C., McKay, C.P., and Joye, S.B. (2010) Abiotic nitrous oxide emissions from the hypersaline Don Juan Pond in Antarctica. Nat Geosci 3: 341-344.
175. Samson, R.A., and van der Lustgraaf, B. (1978) Aspergillus penicilloides and Eurotium halophilicum in association with house-dust mites. Mycopathologia 64: 13-16.
176. Sattler, B., Puxbaum, H., and Psenner, R. (2001) Bacterial growth in supercooled cloud droplets. Geophys Res Lett 28: 239-242.
177. Schubert, B.A., Lowenstein, T.K., Timofeeff, M.N., and Parker, M.A. (2009a) How do prokaryotes survive in fluid inclusions in halite for 30, 000 years? Geology 37: 1059-1062.
178. Schubert, B.A., Lowenstein, T.K., and Timofeeff, M.N. (2009b) Microscopic identification of prokaryotes in modern and ancient halite, Saline Valley and Death Valley, California. Astrobiology 9: 467-482.
179. Sereno, A.M., Hubinger, M.D., Comesaña, J.F., and Correa, A. (2001) Prediction of water activity of osmotic solutions. J Food Eng 49: 103-114.
180. Shomari, S.H., and Kennedy, R. (1999) Survival of Oidium anacardii on cashew (Anacardium occidentale) in southern Tanzania. Plant Pathol 48: 505-513.
181. Siegel, B.Z., McMurty, G., Siegel, S.M., Chen, J., and LaRock, P. (1979) Life in the calcium chloride environment of Don Juan Pond, Antarctica. Nature 280: 828-829.
182. Siegel, B.Z., Siegel, S.M., Chen, J., and LaRock, P. (1983) An extraterrestrial habitat on Earth: the algal mat of Don Juan Pond. Adv Space Res 3: 39-42.
183. Snow, D. (1949) The germination of mould spores at controlled humidities. Ann Appl Biol 36: 1-13.
184. Sohl, F., Choukroun, M., Kargel, J., Kimura, J., Pappalardo, R., Vance, S., and Zolotov, M. (2010) Subsurface water oceans on icy satellites: chemical composition and exchange processes. Space Sci Rev 153: 485-510.
185. Stan-Lotter, H., Radax, C., Gruber, C., McGenity, T.J., Legat, A., Wanner, G., and Denner, E.B.M. (2000) The distribution of viable micro-organisms in Permo-Triassic rock salt. In SALT 2000, 8th World Salt Symposium. Geertman, R.M. (ed.). Amsterdam, The Netherlands: Elsevier Science, pp. 921-926.
186. 38). Curr Microbiol 65: 660-665.
187. Stevenson, A., and Hallsworth, J.E. (2014) Water and temperature relations of soil Actinobacteria. Environ Microbiol Rep doi:10.1111/1758-2229.12199. (in press).
188. Strughold, H. (1953) The Green and the Red Planet: A Physiological Study of the Possibility of Life on Mars. Albuquerque, NM, USA: University of New Mexico Press.
189. Sutton, J.C., and Hildebrand, P.D. (1985) Environmental water in relation to Peronospora destructor and related pathogens. Can J Plant Pathol 7: 323-330.
190. 4 production by a hyperthermophilic methanogen under high-pressure cultivation. Proc Natl Acad Sci USA 105: 10949-10954.
191. Teixeira, S., Olu, K., Decker, C., Cunha, R.L., Fuchs, S., Hourdez, S., etal. (2013) High connectivity across the fragmented chemosynthetic ecosystems of the deep Atlantic Equatorial Belt: efficient dispersal mechanisms or questionable endemism? Mol Ecol 22: 4663-4680.
192. Thomson, W. (1871) Presidential Address to the British Association for the Advancement of Science. Nature 4: 262-270.
193. Toner, J.D., Catling, D.C., and Light, B. (2014) Soluble salts at the Phoenix Lander site, Mars: a reanalysis of the wet chemistry laboratory data. Geochim Cosmochim Acta 136: 142-168.
194. Tosca, N.J., Knoll, A.H., and McLennan, S.M. (2008) Water activity and the challenge for life on early Mars. Science 320: 1204-1207.
195. Trevors, J.T., and Pollack, G.H. (2005) Hypothesis: the origin of life in a hydrogel environment. Prog Biophys Mol Biol 89: 1-8.
196. Troller, J.A., and Christian, J.H.B. (1978) Water Activity and Food. New York, NY, USA: Academic Press.
197. Valentine, D.L. (2013) Microbiology: intraterrestrial lifestyles. Nature 496: 176-177.
198. Von Bloh, W., Cuntz, M., Franck, S., and Bounama, C. (2011) Habitability of the Goldilocks Planet Gliese 581g: results from geodynamic models. Astron Astophys 528: A133.
199. Vreeland, R.H., and Martin, E.L. (1980) Growth characteristics, effects of temperature, and ion specificity of the halotolerant bacterium Halomonas elongata. Can J Microbiol 26: 746-752.
200. Waite, J.H., Combi, M.R., Ip, W.-H., Cravens, T.E., McNutt, R.L., Kasprzak, W., etal. (2006) Cassini ion and neutral mass spectrometer: Enceladus plume composition and structure. Science 311: 1419-1422.
201. Waldham, D.C., and Halvorson, H.O. (1954) Studies on the relationship between equilibrium vapor pressure and moisture content of bacterial endospores. Appl Microbiol 2: 333-338.
202. Warren, J.K. (2010) Evaporites through time: tectonic, climatic and eustatic controls in marine and nonmarine deposits. Earth Sci Rev 98: 217-268.
203. Watanabe, K., and Mizoguchi, M. (2002) Amount of unfrozen water in frozen porous media saturated with solution. Cold Reg Sci Technol 34: 103-110.
204. Westall, F. (2012) Early Earth. In Astrobiology. Lunine, J., etal. (eds). Cambridge, UK: Cambridge University Press, pp. 89-114.
205. Westall, F., de Wit, M.J., Dann, J., van der Gaast, S., de Ronde, C.E.J., and Gerneke, D. (2001) Early Archean fossil bacteria and biofilms in hydrothermally-influenced sediments of the Barberton greenstone belt, South Africa. Precambrian Res 106: 93-116.
206. Westall, F., de Ronde, C.E.J., Southam, G., Grassineau, N., Colas, M., Cockell, C.S., and Lammer, H. (2006) Implications of a 3.472-3.333Gyr-old subaerial microbial mat from the Barberton greenstone belt, South Africa for the UV environmental conditions on the early Earth. Philos Trans R Soc Lond B Biol Sci 361: 1857-1875.
207. Westall, F., Cavalazzi, B., Lemelle, L., Marrocchi, Y., Rouzaud, J.-N., Simionovici, A., etal. (2011) Implications of in situ calcification for photosynthesis in a ~3.3Ga-old microbial biofilm from the Barberton greenstone belt, South Africa. Earth Planet Sci Lett 310: 468-479.
208. Westall, F., Loiseau, D., Foucher, F., Bost, N., Betrand, M., Vago, J., and Kminek, G. (2013) Habitability on Mars from a Microbial point of view. Astrobiology 13: 887-897.
209. van der Wielen, P.W.J.J., Bolhuis, H., Borin, S., Daffonchio, D., Corselli, C., Giuliano, L., etal. (2005) The enigma of prokaryotic life in deep hypersaline anoxic basins. Science 307: 121-123.
210. Wierzchos, J., Cámara, B., de los Rios, A., Davila, A.F., Sánchez Almazo, I.M., Arteida, O., etal. (2011) Microbial colonization of Ca-sulfate crusts in the hyperarid core of the Atacama Desert: implications for the search for life on Mars. Geobiology 9: 44-60.
211. Williams, A.J., Buck, B.J., and Beyene, M.A. (2012) Biological soil crusts in the Mojave Desert, USA: micromorphology and pedogenesis. Soil Sci Soc Am J 76: 1685-1695.
212. Williams, J.P., and Hallsworth, J.E. (2009) Limits of life in hostile environments; no limits to biosphere function? Environ Microbiol 11: 3292-3308.
213. Winston, P.W., and Bates, P.S. (1960) Saturated salt solutions for the control of humidity in biological research. Ecology 41: 232-237.
214. Wolfe, J., Bryant, G., and Koster, K.L. (2002) What is 'unfreezable water', how unfreezable is it and how much is there? CryoLetters 23: 157-166.
215. Womack, A.M., Bohannan, B.J.M., and Green, J.L. (2010) Biodiversity and biogeography of the atmosphere. Philos Trans R Soc Lond B Biol Sci 365: 3645-3653.
216. Womack, A.M., Artaxo, P.E., Ishida, F., Jardine, K.J., Saleska, S.R., Wiedemann, K.T., etal. (2012) Microbial community composition and gene expression in the atmosphere over the Brazilian Amazon. ASM, 112th General Meeting American Society for Microbiology, San Francisco, California.
217. Wyatt, T.T., van Leeuwen, M.R., Gerwig, G.J., Golovina, E.A., Hoekstra, F.A., Kuenstner, E.J., etal. (2014a) Functionality and prevalence of trehalose-based oligosaccharides as novel compatible solutes in ascospores of Neosartorya fischeri (Aspergillus fischeri) and other fungi. Environ Microbiol doi:10.1111/1462-2920.12558. (in press)
218. Wyatt, T.T., Golovina, E.A., van Leeuwen, M.R., Hallsworth, J.E., Wösten, H.A.B., and Dijksterhuis, J. (2014b) Decreases in bulk water and mannitol and accumulation of trehalose and trehalose-based oligosaccharides define a two-stage maturation process towards extreme stress-resistance in ascospores of Neosartorya fischeri (Aspergillus fischeri). Environ Microbiol doi:10.1111/1462-2920.12557. (in press).
219. Wynn-Williams, D.D. (1996) Antarctic microbial diversity: the basis of polar ecosystem processes. Biodivers Conserv 5: 1271-1293.
220. Yakimov, M.M., Lo Cono, V., La Spada, G., Bortoluzzi, G., Messina, E., Smedile, F., etal. (2014) Microbial community of seawater-brine interface of the deep-sea brine Lake Kryos as revealed by recovery of mRNA are active below the chaotropicity limit of life. Environ Microbiol doi:10.1111/1462-2920.12587. (in press).
221. Yarwood, C.E. (1950) Water content of fungus spores. Am J Bot 37: 636-639.
222. Yu, X., Schmidt, A.R., and Schmidt, S.J. (2009) Uncertainty analysis of hygrometer-obtained water activity measurements of saturated salt slurries and food materials. Food Chem 115: 214-226.
223. Zakharova, K., Tesei, D., Marzban, G., Dijksterhuis, J., Wyatt, T., and Sterflinger, K. (2013) Microcolonial fungi on rocks: a life in constant drought? Mycopathologia 175: 537-547.
224. Zolensky, M.E., Bodnar, R.J., Gibson, E.K., Nyquist, L.E., Reese, Y., Shih, C.Y., etal. (1999) Asteroidal water within fluid inclusion-bearing halite in an H5 chondrite, Monahans (1998). Science 285: 1377-1379.
225. Zvyagintsev, D.G., Zenova, G.M., Sudnitsyn, I.I., Gracheva, T.A., Napol'skaya, K.R., and Belousova, M.A. (2009) Dynamics of spore germination and mycelial growth of streptomycetes under low humidity conditions. Microbiology 78: 440-444.
226. Zvyagintsev, D.G., Zenova, G.M., Sudnitsyn, I.I., Gracheva, T.A., Lapygina, E.E., Napol'skaya, K.R., and Sydnitsyna, A.E. (2012) Development of actinomycetes in brown semidesert soil under low water pressure. Eursian Soil Sci 45: 717-725.