Environmental genomics reveals a single-species ecosystem deep within earth

Science

Volumn 322, Issue 5899, 2008, Pages 275-278

  Chivian, Dylan ^a,b   Brodie, Eoin L ^a,b   Alm, Eric J ^b,c   Culley, David E ^d   Dehal, Paramvir S ^a,b   DeSantis, Todd Z ^a,b   Gihring, Thomas M ^e   Lapidus, Alla ^f   Lin, Li Hung ^g   Lowry, Stephen R ^f   Moser, Duane P ^h   Richardson, Paul M ^f   Southam, Gordon ⁱ   Wanger, Greg ⁱ   Pratt, Lisa M ^j,k   Andersen, Gary L ^a,b   Hazen, Terry C ^a,b,k   Brockman, Fred J ^d   Arkin, Adam P ^a,b,l   Onstott, Tullis C ^k,m  

^a LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^b Virtual Institute for Microbial Stress and Survival   (United States)

^c MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^d PACIFIC NORTHWEST NATIONAL LABORATORY   (United States)

^e FLORIDA STATE UNIVERSITY   (United States)

^f DOE JOINT GENOME INSTITUTE   (United States)

^g NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^h DESERT RESEARCH INSTITUTE   (United States)

ⁱ UNIVERSITY OF WESTERN ONTARIO   (Canada)

^j INDIANA UNIVERSITY   (United States)

^k NASA   (United States)

^l University of California at Berkeley   (United States)

^m PRINCETON UNIVERSITY   (United States)

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Author keywords

[No Author keywords available]

Indexed keywords

CARBON; DNA; NITROGEN;

BACTERIUM; BIODIVERSITY; CARBON; DNA; ECOSYSTEM APPROACH; FRACTURE ZONE; GENOMICS; GOLD MINE;

ARTICLE; ASTRONOMY; BACTERIUM ISOLATION; BIOSTRATIGRAPHY; CANDIDATUS DESULFORUDIS AUDAXVIATOR; CHEMOAUTOTROPH; DNA BINDING; EARTH CRUST; ECOLOGICAL GENETICS; GENE SEQUENCE; NONHUMAN; PRIORITY JOURNAL; SINGLE SPECIES ECOSYSTEM; SPECIES IDENTIFICATION; SPOROGENESIS; SULFATE REDUCER; THERMOPHILE;

AMMONIA; CARBON; ECOSYSTEM; GENES, BACTERIAL; GENOME, BACTERIAL; GENOMICS; GOLD; MINING; MOLECULAR SEQUENCE DATA; MOVEMENT; OXIDATION-REDUCTION; PEPTOCOCCACEAE; PHYLOGENY; SEQUENCE ANALYSIS, DNA; SOUTH AFRICA; SPORES, BACTERIAL; SULFATES; TEMPERATURE; WATER MICROBIOLOGY;

BACTERIA (MICROORGANISMS);

EID: 53749096964     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1155495     Document Type: Article

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29. We thank J. Banfield and G. Tyson for helpful discussion; J. Bruckner and B. Baker for assistance with microscopy; F. Warnecke for advice on 16S fluorescent in situ hybridization; T. Kieft, G. Zane, and the MicrobesOnline team (M. Price, K. Keller, and K. Huang) for advice; and D. Kershaw and colleagues at the Mponeng mine and AngloGold Ashanti Limited, RSA. This work was part of the Virtual Institute for Microbial Stress and Survival (http://vimss.lbl.gov), supported by DOE, Office of Science, Office of Biological and Environmental Research, Genomics Program:GTL through contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and DOE. This work was also supported by the NASA Astrobiology Institute through award NNA04CC03A to the IPTAI Team co-directed by L.M.P. and T.C.O. A.P.A. received support from the Howard Hughes Medical Institute. The genome sequence and 16S library sequences reported in this study have been deposited in GenBank under the accession numbers CP000860 and EU730965 to EU731008, respectively.