High-throughput mAb expression and purification platform based on transient CHO

Biotechnology Progress

Volumn 31, Issue 1, 2015, Pages 239-247

  Barnard, Gavin C ^a   Hougland, Maria D ^a   Rajendra, Yashas ^a  

^a ELI LILLY AND COMPANY   (United States)

Author keywords

High throughput purification; Protein A; Transient CHO

Indexed keywords

CELL CULTURE; MOLECULAR BIOLOGY; MONOCLONAL ANTIBODIES; PURIFICATION;

AFFINITY MATURATION; AVERAGE CONCENTRATION; HIGH-THROUGHPUT; LIQUID HANDLING ROBOTS; PROTEIN A; PROTEIN CONCENTRATIONS; PURIFICATION PROCESS; TRANSIENT TRANSFECTION;

THROUGHPUT;

MONOCLONAL ANTIBODY; STAPHYLOCOCCUS PROTEIN A;

ANIMAL; CHEMISTRY; CHO CELL LINE; CRICETULUS; CULTURE TECHNIQUE; HAMSTER; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; ISOLATION AND PURIFICATION; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROCEDURES;

ANIMALS; ANTIBODIES, MONOCLONAL; CELL CULTURE TECHNIQUES; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRICETINAE; CRICETULUS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HIGH-THROUGHPUT SCREENING ASSAYS; STAPHYLOCOCCAL PROTEIN A;

EID: 84923413480     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.2012     Document Type: Article

References (33)

1. FiercePharma. The 10 best-selling drugs of 2013. Available at http://www.fiercepharma.com/special-reports/10-best-selling-drugs-2013 [Accessed August 26, 2014].
2. Forbes. The best selling drugs of all time; Humira joins the elite. Available at http://www.forbes.com/ sites/simonking/2013/01/28/the-best-selling-drugs-of-all-time-humira-joins-the-elite/ [Accessed August 26, 2014].
3. PhRMA, The pharmaceutical research and manufacturers of America. Medicines in development for biologics. Available at http://www.phrma.org/medicines-development-biologics [Accessed August 26, 2014].
4. Chon JH, Zarbis-Papastoitsis G. Advances in the production and downstream processing of antibodies. New Biotechnol. 2011;28:458-463.
5. Kessler SW. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: Parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975;115:1617-1624.
6. Ey PL, Prowse SJ, Jenkin CR. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose. Immunochem. 1978;7:429-436.
7. Langone JJ. Protein A of Staphylococcus aureus and related immunoglobulin receptors produced by streptococci and pneumonococci. Adv Immunol. 1982;32:157-252.
8. Braisted AC, Wells JA. Minimizing a binding domain from protein A. Proc Natl Acad Sci USA. 1996;93:5688-5692.
9. Saha K, Bender F, Gizeli E. Comparative study of IgG binding to proteins G and A: Nonequilibrium kinetic and binding constant determination with the acoustic waveguide device. Anal Chem. 2003;75:835-842.
10. Linköping University, Department of Physics, Chemistry and Biology. Maters Thesis by Sofia Nohldén. Affinity Determination of Protein A Domains to IgG subclasses by Surface Plasmon Resonance. Available at http://www.diva-portal.org/smash/get/diva2:17753/FULLTEXT01.pdf [Accessed August 26, 2014].
11. GE Healthcare Life Sciences. Dynamic binding capacity study on MabSelect SuRe™ LX for capturing high-titer monoclonal antibodies. Available at http://www.gelifesciences.com/gehcls_images/GELS/ Related%20Content/Files/1314807262343/litdoc28987525_20140224214140.pdf [Accessed August 26, 2014].
12. Nelson AL, Dhimolea E, Reichert JM. Outlook: Development trends for human monoclonal antibody therapeutics. Nat Rev Drug Discovery. 2010;9:767-774.
13. Kellermann SA, Green LL. Antibody discovery: The use of transgenic mice to generate human monoclonal antibodies for therapeutics. SA, LL Green. Curr Opin Biotechnol. 2002;13:593-597.
14. Harmsen MM, De Haard HJ. Properties, production, and applications of camelid single-domain antibody fragments. Appl.Microbiol.Biotechnol. 2007;77:13-22.
15. Bradbury ARM. Beyond natural antibodies: The power of in vitro display technologies. Nature Biotechnol. 2011;29:245-254.
16. Kretzschmar T, von Rüden T. Antibody discovery: Phage display. Curr Opin Biotechnol. 2002;13:598-602.
17. Winter G, Griffiths AD, Hawkins RE, Hoogenboom HR. Making Antibodies by Phage Display Technology. Annu Rev Immunol. 1994;12:433-455.
18. Lipovsek D, Plückthun A. In-vitro protein evolution by ribosome display and mRNA display. J Immunol Methods. 2004;290:51-67.
19. Presta LG. Molecular engineering and design of therapeutic antibodies. Curr Opin Immunol. 2008;20:460-470.
20. Chan AC, Carter PJ. Therapeutic antibodies for autoimmunity and inflammation. Nat. Rev. Immunol. 2010;10:301-316.
21. Mallaney M, Wang SH, Sreedhara A. Effect of ambient light on monoclonal antibody product quality during small-scale mammalian cell culture process in clear glass bioreactors. Biotechnol Prog. 2014;30:562-570.
22. Backliwal G, Hildinger M, Chenuet S, Wulhfard S, De Jesus M, Wurm FM. Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions. Nucleic Acids Res. 2008;36:e96-e96.
23. Rajendra Y, Kiseljak D, Baldi L, Hacker DL, Wurm FM. A simple high-yielding process for transient gene expression in CHO cells. J Biotechnol. 2011;153:22-26.
24. Rajendra Y, Kiseljak D, Manoli S, Baldi L, Hacker DL, Wurm FM. Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells. Biotechnol Bioeng. 2012;109:2271-2278.
25. Daramola O, Stevenson J, Dean G, Hatton D, Pettman G, Holmes W, Field R. A high-yielding CHO transient system: Coexpression of genes encoding EBNA-1 and GS enhances transient protein expression. Biotechnol Prog. 2014;30:132-141.
26. Cain K, Peters S, Hailu H, Sweeney B, Stephens P, Heads J, Sarkar K, Ventom A, Page C, Dickson A. A CHO cell line engineered to express XBP1 and ERO1-Lα has increased levels of transient protein expression. Biotechnol Prog. 2013;29:697-706.
27. Bos AB, Duque JN, Bhakta S, Farahi F, Chirdon LA, Junutula JR, Harms PD, Wong AW. Development of a semi-automated high throughput transient transfection system. J Biotechnol. 2014;180:10-16.
28. Hopp J, Pritchett R, Darlucio M, Ma J, Chou JH. Development of a high throughput protein: A well-plate purification method for monoclonal antibodies. Biotechnol Prog. 2009;5:1427-1432.
29. Jiang Y, Li F, Button M, Cukan M, Moore R, Sharkey N, Li H. A high-throughput purification of monoclonal antibodies from glycoengineered Pichia pastoris. Protein Expr Purif. 2010;74:9-15.
30. Reusch D, Haberger M, Selman MH, Bulau P, Deelder AM, Wuhrer M, Engler N. High-throughput work flow for IgG Fc-glycosylation analysis of biotechnological samples. Anal Biochem. 2013;432:82-89.
31. Bailey MJ, Hooker AD, Adams CS, Zhang S, James DC. A platform for high-throughput molecular characterization of recombinant monoclonal antibodies. J Chromatogr B Analyt Technol Biomed Life Sci. 2005;826:177-187.
32. Fan L, Kadura I, Krebs LE, Hatfield CC, Shaw MM, Frye CC. Improving the efficiency of CHO cell line generation using glutamine synthetase gene knockout cells. Biotechnol. Bioeng. 2012;109:1007-1015.
33. Fan L, Kadura I, Krebs LE, Larson JL, Bowden DM, Frye CC. Development of a highly-efficient CHO cell line generation system with engineered SV40E promoter. J Biotechnol. 2013;168:652-658.