High-throughput work flow for IgG Fc-glycosylation analysis of biotechnological samples

Analytical Biochemistry

Volumn 432, Issue 2, 2013, Pages 82-89

  Reusch, Dietmar ^a   Haberger, Markus ^a   Selman, Maurice H J ^b   Bulau, Patrick ^a   Deelder, André M ^b   Wuhrer, Manfred ^b   Engler, Niklas ^a  

^a ROCHE DIAGNOSTICS GMBH   (Germany)

^b LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Automation; Biotechnological samples; Electrospray mass spectrometry; Glycopeptides; IgG glycosylation; Recombinant monoclonal antibodies

Indexed keywords

AUTOMATION; BIOTECHNOLOGY; CYTOTOXICITY; FERMENTATION; HYDROPHILICITY; MASS SPECTROMETRY; MONOCLONAL ANTIBODIES; PEPTIDES; PHASE SEPARATION; POSITIVE IONS; PURIFICATION;

ANTIBODY-DEPENDENT CELLULAR CYTOTOXICITIES; COMPLEMENT-DEPENDENT CYTOTOXICITIES; ELECTROSPRAY MASS SPECTROMETRY; GLYCOPEPTIDES; HIGH-THROUGHPUT METHOD; HIGH-THROUGHPUT TECHNIQUE; HYDROPHILIC INTERACTION; RECOMBINANT MONOCLONAL ANTIBODY;

GLYCOSYLATION;

GLYCOPEPTIDE; IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN G1 ANTIBODY; PROTEIN A; TRYPSIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BIOTECHNOLOGICAL PROCEDURES; BROTH DILUTION; CONTROLLED STUDY; CRYSTALLIZATION; ELECTROSPRAY MASS SPECTROMETRY; FEMALE; HIGH THROUGHPUT SCREENING; HYDROPHILIC INTERACTION CHROMATOGRAPHY; NONHUMAN; POSITIVE ION ELECTROSPRAY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN GLYCOSYLATION; SOLID PHASE EXTRACTION;

EID: 84868553080     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.09.032     Document Type: Article

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