Development of a high throughput protein a well-plate purification method for monoclonal antibodies

Biotechnology Progress

Volumn 25, Issue 5, 2009, Pages 1427-1432

  Hopp, Jennifer ^a   Pritchett, Ross ^a   Darlucio, Maria ^a   Ma, Junfen ^a   Chou, Judy H ^a  

^a GENENTECH INC   (United States)

Author keywords

High throughput; Monoclonal antibody purification; Protein A

Indexed keywords

96-WELL; ANALYTICAL CHARACTERIZATION; CAPILLARY ISOELECTRIC FOCUSING; CAPILLARY ZONE ELECTROPHORESIS; CATION EXCHANGE CHROMATOGRAPHY; HIGH THROUGHPUT; MONOCLONAL ANTIBODY PURIFICATION; NEW HIGH; PROTEIN A; PROTEIN LOADINGS; PURIFICATION METHOD; TRYPTIC DIGESTS;

ALLERGIES; CAPILLARY ELECTROPHORESIS; CELL CULTURE; CELL MEMBRANES; CHROMATOGRAPHIC ANALYSIS; MERGERS AND ACQUISITIONS; MONOCLONAL ANTIBODIES; RESINS; SIZE EXCLUSION CHROMATOGRAPHY; SURFACE PLASMON RESONANCE; THROUGHPUT;

PURIFICATION;

IMMUNOGLOBULIN G; ION EXCHANGE RESIN; MONOCLONAL ANTIBODY; RECOMBINANT PROTEIN; STAPHYLOCOCCUS PROTEIN A;

AFFINITY CHROMATOGRAPHY; ARTICLE; CHEMISTRY; GEL CHROMATOGRAPHY; HUMAN; INSTRUMENTATION; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METHODOLOGY;

ANTIBODIES, MONOCLONAL; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; HUMANS; IMMUNOGLOBULIN G; ION EXCHANGE RESINS; RECOMBINANT PROTEINS; STAPHYLOCOCCAL PROTEIN A;

EID: 70350144670     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1002/btpr.247     Document Type: Article

References (14)

1. Fahrner RL, Knudsen HL, Basey CD, Galan W, Feuerhelm D, Vanderlaan M, Blank GS. Industrial purification of pharmaceutical antibodies: development, operation, and validation of chromatography processes. Biotechnol Genet Eng Rev. 2001;18:302-327.
2. Shukla AA, Hubbard B, Tressel T, Guhan S, Low D. Downstream processing of monoclonal antibodies-application of platform approaches. J Chromatogr B. 2007;848:28-39.
3. Londo T, Lynch P, Kehoe T, Meys M, Gordon N. Accelerated recombinant protein purification process development: automated, robotics-based integration of chromatographic purification and analysis. J Chromatogr A. 1998;798:73-82.
4. Rege K, Tugcu N, Cramer SM. Predicting column performance in displacement chromatography from high throughput screening batch experiments. Sep Sci Technol. 2003;38:1499-1517.
5. Rege K, Pepsin M, Falcon B, Steele L, Heng M. High-throughput process development for recombinant protein purification. Biotechnol Bioeng. 2006;93:618-630.
6. Thiemann J, Jankowski J, Rykl J, Kurzawski S, Pohl T, Wittmann-Liebold B, Schluter H. Principle and applications of the protein-purification-parameter screening system. J Chromatogr A. 2004;1043:73-80.
7. Rege K, Ladiwala A, Cramer SM. Multidimensional high-throughput screening of displacers. Anal Chem. 2005:77:6818-6827.
8. Herrmann T, Schroder M, Hubbuch J. Generation of equally sized particle plaques using solid-liquid suspensions. Biotechnol Prog. 2006;22:914-918.
9. Stein A, Kiesewetter A. Cation exchange chromatography in antibody purification: pH screening for optimized binding and HCP removal. J Chromatogr B. 2007;848:151-158.
10. Staby A, Jensen RH, Bensch M, Hubbuch J, Dunweber DL, Krarup J, Nielsen J, Lund M, Kidal S, Hansen TB, Jensen IH. Comparison of chromatographic ion-exchange resins. VI. Weak anion-exchange resins. J Chromatogr A. 2007;1164:82-94.
11. Kelley BD, Switzer M, Bastek P, Kramarczyk JF, Molnar K, Yu T, Coffman J. High-throughput screening of chromatographic separations. IV. Ion-exchange. Biotechnol Bioeng. 2008; 100:950-963.
12. Schulze-Wierling P, Bogumil R, Knieps-Grunhagen E, Hubbuch J. High-throughput screening of packed-bed chromatography coupled with SELDI-TOF MS analysis: monoclonal antibodies versus host cell protein. Biotechnol Bioeng. 2007;98:440-450.
13. Bailey MJ, Hooker AD, Adams CS, Zhang S, James DC. A platform for high-throughput molecular characterization of recombinant monoclonal antibodies. J Chromatogr B. 2005;826: 177-187.
14. Wuhrer M, Stam JC, van de Geijn FE, Koeleman CAM, Verrips CT, Dolhain JEM, Hokke CH, Deelder AM. Glycosylation profiling of immunoglobulin G (IgG) subclasses from human serum. Proteomics 2007;7:4070-4081.