Effects of temperature and glycerol and methanol-feeding profiles on the production of recombinant galactose oxidase in Pichia pastoris

Biotechnology Progress

Volumn 30, Issue 3, 2014, Pages 728-735

  Anasontzis, George E ^a   Penã, Margarita Salazar ^a,c   Spadiut, Oliver ^b,d   Brumer, Harry ^b,e   Olsson, Lisbeth ^a  

^a CHALMERS UNIVERSITY OF TECHNOLOGY   (Sweden)

^b ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^c NOVOZYMES A S   (Denmark)

^d Inst Verfahrenstechnik U   (Austria)

^e UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Fusarium graminearum; Galactose oxidase; Methanol feeding; Optimization; Pichia pastoris

Indexed keywords

GLYCEROL; METHANOL; OPTIMIZATION; PRODUCTIVITY; RECOMBINANT PROTEINS; TEMPERATURE; YEAST;

BIOMASS CONCENTRATIONS; EFFECTS OF TEMPERATURE; EXPONENTIAL FEEDING; FUSARIUM GRAMINEARUM; GALACTOSE OXIDASE; PICHIA PASTORIS; PROTEIN PRODUCTION; VOLUMETRIC PRODUCTIVITY;

FEEDING;

GIBBERELLA ZEAE; PICHIA PASTORIS;

GALACTOSE OXIDASE; GLYCEROL; METHANOL; RECOMBINANT PROTEIN;

BIOSYNTHESIS; ENZYMOLOGY; FERMENTATION; FUSARIUM; GENE EXPRESSION REGULATION; GENETICS; PICHIA; TEMPERATURE;

FERMENTATION; FUSARIUM; GALACTOSE OXIDASE; GENE EXPRESSION REGULATION, FUNGAL; GLYCEROL; METHANOL; PICHIA; RECOMBINANT PROTEINS; TEMPERATURE;

EID: 84922969799     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.1878     Document Type: Article

References (48)

1. Avigad G, Amaral D, Asensio C, Horecker BL. The D-galactose oxidase of Polyporus circinatus. J Biol Chem. 1962;237:2736-2743.
2. Sun L, Petrounia IP, Yagasaki M, Bandara G, Arnold FH. Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution. Protein Eng. 2001;14:699-704.
3. Whittaker JW. Free radical catalysis by galactose oxidase. Chem Rev. 2003;103:2347-2363.
4. Whittaker JW. The radical chemistry of galactose oxidase. Arch Biochem Biophys. 2005;433:227-239.
5. Baron AJ, Stevens C, Wilmot C, Seneviratne KD, Blakeley V, Dooley DM, Phillips SE, Knowles PF, McPherson MJ. Structure and mechanism of galactose oxidase. The free radical site. J Biol Chem. 1994;269:25095-25105.
6. Kosman DJ, Ettinger MJ, Weiner RE, Massaro EJ. The molecular properties of the copper enzyme galactose oxidase. Arch Biochem Biophys. 1974;165:456-467.
7. Rogers MS, Tyler EM, Akyumani N, Kurtis CR, Spooner RK, Deacon SE, Tamber S, Firbank SJ, Mahmoud K, Knowles PF, Phillips SE, McPherson MJ, Dooley DM. The stacking tryptophan of galactose oxidase: A second-coordination sphere residue that has profound effects on tyrosyl radical behavior and enzyme catalysis. Biochemistry 2007;46:4606-4618.
8. Ito N, Phillips SE, Stevens C, Ogel ZB, McPherson MJ, Keen JN, Yadav KD, Knowles PF. Novel thioether bond revealed by a 1.7 A crystal structure of galactose oxidase. Nature 1991;350: 87-90.
9. Deacon SE, Mahmoud K, Spooner RK, Firbank SJ, Knowles PF, Phillips SE, McPherson MJ. Enhanced fructose oxidase activity in a galactose oxidase variant. ChemBioChem 2004;5: 972-979.
10. McFaul SJ, Lin H, Everse J. The mechanism of peroxidasemediated cytotoxicity. I. Comparison of horseradish peroxidase and lactoperoxidase. Proc Soc Exp Biol Med. 1986;183:244-249.
11. Majerus PM, Courtois PA. Susceptibility of Candida albicans to peroxidase-catalyzed oxidation products of thiocyanate, iodide and bromide. J Biol Buccale. 1992;20:241-245.
12. Szabo EE, Adanyi N, Varadi M. Application of biosensor for monitoring galactose content. Biosens Bioelectronics. 1996;11: 1051-1058.
13. Adányi N, Szabó EE, Váradi M. Multi-enzyme biosensors with amperometric detection for determination of lactose in milk and dairy products. Eur Food Res Technol 1999;209:220-226.
14. Amárita Vega F, Núñez CG, Weigel B, Hitzmann B, Diaz Ricci JC. On-line monitoring of galactoside conjugates and glycerol by flow injection analysis. Anal Chim Acta 1998;373:57-62.
15. Kinoshita M, Inagake K, Kawabata A, Kuroda R, Oda Y, Kakehi K. Fluorometric determination of mucin-type glycoproteins by the galactose oxidase-peroxidase method. Anal Biochem. 2000;284:87-92.
16. Henderson GE, Isett KD, Gerngross TU. Site-specific modification of recombinant proteins: a novel platform for modifying glycoproteins expressed in E. coli. Bioconjug Chem. 2011;22:903-912.
17. Said IT, Shamsuddin AM, Sherief MA, Taleb SG, Aref WF, Kumar D. Comparison of different techniques for detection of Gal-GalNAc, an early marker of colonic neoplasia. Histology Histopathol. 1999;14:351-357.
18. Carter JH, Deddens JA, Pullman JL, Colligan BM, Whiteley LO, Carter HW. Validation of the galactose oxidase-Schiff's reagent sequence for early detection and prognosis in human colorectal adenocarcinoma. Clin Cancer Res. 1997;3:1479-1489.
19. Leppänen A-S, Xu C, Parikka K, Eklund P, Sjöholm R, Brumer H, Tenkanen M, Willför S. Targeted allylation and propargylation of galactose-containing polysaccharides in water. Carbohydr Polym. 2014;100:46-54.
20. Parikka K, Leppanen AS, Xu C, Pitkanen L, Eronen P, Osterberg M, Brumer H, Willfor S, Tenkanen M. Functional and anionic cellulose-interacting polymers by selective chemoenzymatic carboxylation of galactose-containing polysaccharides. Biomacromolecules 2012;13:2418-2428.
21. Xu C, Spadiut O, Araujo AC, Nakhai A, Brumer H. Chemoenzymatic assembly of clickable cellulose surfaces via multivalent polysaccharides. ChemSusChem 2012;5:661-665.
22. Lang P, Masci G, Dentini M, Crescenzi V, Cooke D, Gidley MJ, Fanutti C, Reid JSG. Tamarind seed polysaccharide: preparation, characterisation and solution properties of carboxylated, sulphated and alkylaminated derivatives. Carbohydr Polym. 1992;17:185-198.
23. Yalpani M, Hall LD. Some chemical and analytical aspects of polysaccharide modifications. II. A high-yielding, specific method for the chemical derivatization of galactose-containing polysaccharides: Oxidation with galactose oxidase followed by reductive amination. J Polym Sci: Polym Chem Ed. 1982;20:3399-3420.
24. Kupper CE, Rosencrantz RR, Henssen B, Pelantova H, Thones S, Drozdova A, Kren V, Elling L. Chemo-enzymatic modification of poly-N-acetyllactosamine (LacNAc) oligomers and N,Ndiacetyllactosamine (LacDiNAc) based on galactose oxidase treatment. Beilstein J Org Chem. 2012;8:712-725.
25. Escalettes F, Turner NJ. Directed evolution of galactose oxidase: Generation of enantioselective secondary alcohol oxidases. ChemBioChem 2008;9:857-860.
26. Deacon SE, McPherson MJ. Enhanced expression and purification of fungal galactose oxidase in Escherichia coli and use for analysis of a saturation mutagenesis library. ChemBioChem 2011;12:593-601.
27. Sun L, Bulter T, Alcalde M, Petrounia IP, Arnold FH. Modification of galactose oxidase to introduce glucose 6-oxidase activity. ChemBioChem 2002;3:781-783.
28. Rannes JB, Ioannou A, Willies SC, Grogan G, Behrens C, Flitsch SL, Turner NJ. Glycoprotein labeling using engineered variants of galactose oxidase obtained by directed evolution. J Am Chem Soc. 2011;133:8436-8439.
29. Whittaker MM, Whittaker JW. Expression of recombinant galactose oxidase by Pichia pastoris. Protein Expr Purif. 2000;20: 105-111.
30. Spadiut O, Olsson L, Brumer H III. A comparative summary of expression systems for the recombinant production of galactose oxidase. Microb Cell Fact. 2010;9:68.
31. Higgins D, Cregg J. Pichia Protocols. Humana Press; Totowa, New Jersey, 1998;103:270.
32. Cregg JM, Cereghino JL, Shi J, Higgins DR. Recombinant protein expression in Pichia pastoris. Mol Biotechnol. 2000;16:23-52.
33. Zhang AL, Luo JX, Zhang TY, Pan YW, Tan YH, Fu CY, Tu FZ. Recent advances on the GAP promoter derived expression system of Pichia pastoris. Mol Biol Rep. 2009;36:1611-1619.
34. Couderc R, Baratti J. Oxidation of Methanol by the Yeast, Pichia pastoris. Purification and properties of the alcohol oxidase. Agric Biol Chem. 1980;44:2279-2289.
35. Trinh LB, Phue JN, Shiloach J. Effect of methanol feeding strategies on production and yield of recombinant mouse endostatin from Pichia pastoris. Biotechnol Bioeng. 2003;82:438-444.
36. Li ZM, Ping XB, Ye Q, Huang XD, Cao ZF. Production and optimization of recombinant human augmenter of liver regeneration by Pichia pastoris. Enzyme Microb Technol. 2010;47:222-227.
37. Zhang W, Bevins MA, Plantz BA, Smith LA, Meagher MM. Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy-chain fragment C of botulinum neurotoxin, serotype A. Biotechnol Bioeng. 2000;70:1-8.
38. Zhang W, Inan M, Meagher MM. Rational design and optimization of fed-batch and continuous fermentations. In: Cregg JM, editor. Pichia Protocols. Humana Press; 2007;389:43-63.
39. Bushell ME, Rowe M, Avignone-Rossa CA, Wardell JN. Cyclic fed-batch culture for production of human serum albumin in Pichia pastoris. Biotechnol Bioeng. 2003;82:678-683.
40. Li J, Tang C, Shi H, Wu M. Cloning and optimized expression of a neutral endoglucanase gene (ncel5A) from Volvariella volvacea WX32 in Pichia pastoris. J Biosci Bioeng. 2011;111:537-540.
41. Dragosits M, Frascotti G, Bernard-Granger L, Vazquez F, Giuliani M, Baumann K, Rodriguez-Carmona E, Tokkanen J, Parrilli E, Wiebe MG, Kunert R, Maurer M, Gasser B, Sauer M, Branduardi P, Pakula T, Saloheimo M, Penttila M, Ferrer P, Luisa Tutino M, Villaverde A, Porro D, Mattanovich D. Influence of growth temperature on the production of antibody Fab fragments in different microbes: A host comparative analysis. Biotechnol Prog. 2011;27:38-46.
42. Batra G, Gurramkonda C, Nemani SK, Jain SK, Swaminathan S, Khanna N. Optimization of conditions for secretion of dengue virus type 2 envelope domain III using Pichia pastoris. J Biosci Bioeng. 2010;110:408-414.
43. Jungo C, Marison I, von Stockar U. Mixed feeds of glycerol and methanol can improve the performance of Pichia pastoris cultures: A quantitative study based on concentration gradients in transient continuous cultures. J Biotechnol. 2007;128:824-837.
44. Wang Y, Wang Z, Du G, Hua Z, Liu L, Li J, Chen J. Enhancement of alkaline polygalacturonate lyase production in recombinant Pichia pastoris according to the ratio of methanol to cell concentration. Bioresour Technol. 2009;100:1343-1349.
45. Dietzsch C, Spadiut O, Herwig C. A dynamic method based on the specific substrate uptake rate to set up a feeding strategy for Pichia pastoris. Microb Cell Fact 2011;10:14.
46. Dietzsch C, Spadiut O, Herwig C. A fast approach to determine a fed batch feeding profile for recombinant Pichia pastoris strains. Microb Cell Fact. 2011;10:85.
47. Cunha AE, Clemente JJ, Gomes R, Pinto F, Thomaz M, Miranda S, Pinto R, Moosmayer D, Donner P, Carrondo MJ. Methanol induction optimization for scFv antibody fragment production in Pichia pastoris. Biotechnol Bioeng. 2004;86:458-467.
48. Zhou XS, Fan WM, Zhang YX. Effects of different methanol feeding strategy on hirudin production in high-density fermentation by recombinant Pichia pastoris. Chin J Biotechnol. 2002; 18: 348-351.