Cloning and optimized expression of a neutral endoglucanase gene (ncel5A) from Volvariella volvacea WX32 in Pichia pastoris

Journal of Bioscience and Bioengineering

Volumn 111, Issue 5, 2011, Pages 537-540

  Li, Jianfang ^a   Tang, Cunduo ^a   Shi, Hongling ^a   Wu, Minchen ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Endoglucanase optimized expression; Enzymatic properties; Gene cloning; Pichia pastoris; Volvariella volvacea

Indexed keywords

ENDOGLUCANASES; ENZYMATIC PROPERTIES; GENE CLONING; PICHIA PASTORIS; VOLVARIELLA VOLVACEA;

AMINO ACIDS; CLONING; DIFFUSERS (OPTICAL); ENZYME ACTIVITY; OPTIMIZATION; SUGARS;

GENE EXPRESSION;

ANTIBIOTIC G 418; COMPLEMENTARY DNA; DNA FRAGMENT; GLUCAN SYNTHASE; GLYCOSYLATED PROTEIN; PROTEIN NCEL5A; UNCLASSIFIED DRUG;

ARTICLE; ELECTROPORATION; ENZYME ACTIVITY; GENE ACTIVITY; GENE EXPRESSION; GENETIC SCREENING; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; ONLINE ANALYSIS; PH; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; RECOMBINANT PLASMID; SEQUENCE HOMOLOGY; VOLVARIELLA; VOLVARIELLA VOLVACEA;

CELLULASE; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ELECTROPORATION; ENZYME STABILITY; FUNGAL PROTEINS; GENE EXPRESSION; GLYCOSYLATION; HYDROGEN-ION CONCENTRATION; PICHIA; SEQUENCE HOMOLOGY, AMINO ACID; VOLVARIELLA;

PICHIA PASTORIS; VOLVARIELLA VOLVACEA;

EID: 79954720385     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2011.01.002     Document Type: Article

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