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Volumn 12, Issue 4, 2011, Pages 593-601

Enhanced Expression and Purification of Fungal Galactose Oxidase in Escherichia coli and Use for Analysis of a Saturation Mutagenesis Library

(2) Deacon, Sarah E a McPherson, Michael J a

a UNIVERSITY OF LEEDS (United Kingdom)

Author keywords

Escherichia coli; Galactose oxidase; Metalloenzymes; Protein expression; Saturation mutagenesis

Indexed keywords

GALACTOSE OXIDASE; MESSENGER RNA; RECOMBINANT ENZYME;

ARTICLE; BACTERIAL STRAIN; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CYTOLYSIS; CYTOPLASM; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME RELEASE; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; GENE MUTATION; GENE SEQUENCE; GENETIC TRANSCRIPTION; HOST; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; RNA STRUCTURE; RNA TRANSLATION; TEMPERATURE;

BASE SEQUENCE; CATALYTIC DOMAIN; ESCHERICHIA COLI; FUSARIUM; GALACTOSE OXIDASE; GENE EXPRESSION REGULATION, ENZYMOLOGIC; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; MUTAGENESIS; SMALL MOLECULE LIBRARIES;

ESCHERICHIA COLI; FUNGI; FUSARIUM;

EID: 79953121726 PISSN: 14394227 EISSN: 14397633 Source Type: Journal
DOI: 10.1002/cbic.201000634 Document Type: Article

Times cited : (22)

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