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Volumn 118, Issue 3, 2015, Pages 386-394

GAPDH and β-actin protein decreases with aging, making Stain-Free technology a superior loading control in Western blotting of human skeletal muscle

Author keywords

Data normalization; Housekeeping protein; Human skeletal muscle; Reference protein; Stain Free technology; Western blot

Indexed keywords

ACTIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; PROTEIN;

EID: 84922348321     PISSN: 87507587     EISSN: 15221601     Source Type: Journal    
DOI: 10.1152/japplphysiol.00840.2014     Document Type: Article
Times cited : (99)

References (32)
  • 1
    • 46249108210 scopus 로고    scopus 로고
    • The use of total protein stains as loading controls: An alternative to high-abundance single-protein controls in semi-quantitative immunoblotting
    • Aldridge GM, Podrebarac DM, Greenough WT, Weiler IJ. The use of total protein stains as loading controls: an alternative to high-abundance single-protein controls in semi-quantitative immunoblotting. J Neurosci Methods 172: 250-254, 2008.
    • (2008) J Neurosci Methods , vol.172 , pp. 250-254
    • Aldridge, G.M.1    Podrebarac, D.M.2    Greenough, W.T.3    Weiler, I.J.4
  • 3
    • 0036551118 scopus 로고    scopus 로고
    • The light-induced reactions of tryptophan with halocompounds
    • Edwards RA, Jickling G, Turner RJ. The light-induced reactions of tryptophan with halocompounds. Photochem Photobiol 75: 362-368, 2002.
    • (2002) Photochem Photobiol , vol.75 , pp. 362-368
    • Edwards, R.A.1    Jickling, G.2    Turner, R.J.3
  • 4
    • 13844313879 scopus 로고    scopus 로고
    • Housekeeping proteins: A preliminary study illustrating some limitations as useful references in protein expression studies
    • Ferguson RE, Carroll HP, Harris A, Maher ER, Selby PJ, Banks RE. Housekeeping proteins: a preliminary study illustrating some limitations as useful references in protein expression studies. Proteomics 5: 566-571, 2005.
    • (2005) Proteomics , vol.5 , pp. 566-571
    • Ferguson, R.E.1    Carroll, H.P.2    Harris, A.3    Maher, E.R.4    Selby, P.J.5    Banks, R.E.6
  • 7
    • 84884964957 scopus 로고    scopus 로고
    • Stain-Free total protein staining is a superior loading control to beta-actin for Western blots
    • Gilda JE, Gomes AV. Stain-Free total protein staining is a superior loading control to beta-actin for Western blots. Anal Biochem 440: 186-188, 2013.
    • (2013) Anal Biochem , vol.440 , pp. 186-188
    • Gilda, J.E.1    Gomes, A.V.2
  • 8
    • 84907963778 scopus 로고    scopus 로고
    • Two weeks of one-leg immobilization decreases skeletal muscle respiratory capacity equally in young and elderly men
    • Gram M, Vigelso A, Yokota T, Hansen CN, Helge JW, Hey-Mogensen M, Dela F. Two weeks of one-leg immobilization decreases skeletal muscle respiratory capacity equally in young and elderly men. Exp Gerontol 58C: 269-278, 2014.
    • (2014) Exp Gerontol , vol.58 C , pp. 269-278
    • Gram, M.1    Vigelso, A.2    Yokota, T.3    Hansen, C.N.4    Helge, J.W.5    Hey-Mogensen, M.6    Dela, F.7
  • 10
    • 84897532701 scopus 로고    scopus 로고
    • A method for greater reliability in Western blot loading controls - Stain-Free total protein quantitation
    • Hammond M, Kohn J, Oh K, Piatti P, Liu N. A method for greater reliability in Western blot loading controls - Stain-Free total protein quantitation. Bio-Rad Bull 6360: 18-21, 2013.
    • (2013) Bio-Rad Bull , vol.6360 , pp. 18-21
    • Hammond, M.1    Kohn, J.2    Oh, K.3    Piatti, P.4    Liu, N.5
  • 11
    • 0030991893 scopus 로고    scopus 로고
    • Decline in isokinetic force with age: Muscle cross-sectional area and specific force
    • Jubrias SA, Odderson IR, Esselman PC, Conley KE. Decline in isokinetic force with age: muscle cross-sectional area and specific force. Pflügers Arch 434: 246-253, 1997.
    • (1997) Pflügers Arch , vol.434 , pp. 246-253
    • Jubrias, S.A.1    Odderson, I.R.2    Esselman, P.C.3    Conley, K.E.4
  • 12
    • 84875720543 scopus 로고    scopus 로고
    • An old method facing a new challenge: Re-visiting housekeeping proteins as internal reference control for neuroscience research
    • Li R, Shen Y. An old method facing a new challenge: re-visiting housekeeping proteins as internal reference control for neuroscience research. Life Sci 92: 747-751, 2013.
    • (2013) Life Sci , vol.92 , pp. 747-751
    • Li, R.1    Shen, Y.2
  • 13
    • 0034011369 scopus 로고    scopus 로고
    • Glyceraldehyde-3-phosphate dehydrogenase varies with age in glycolytic muscles of rats
    • Lowe DA, Degens H, Chen KD, Alway SE. Glyceraldehyde-3-phosphate dehydrogenase varies with age in glycolytic muscles of rats. J Gerontol A Biol Sci Med Sci 55: B160-164, 2000.
    • (2000) J Gerontol A Biol Sci Med Sci , vol.55 , pp. B160-B164
    • Lowe, D.A.1    Degens, H.2    Chen, K.D.3    Alway, S.E.4
  • 15
    • 84888439104 scopus 로고    scopus 로고
    • Important considerations for protein analyses using antibody based techniques: Down-sizing Western blotting up-sizes outcomes
    • Murphy RM, Lamb GD. Important considerations for protein analyses using antibody based techniques: down-sizing Western blotting up-sizes outcomes. J Physiol 591: 5823-5831, 2013.
    • (2013) J Physiol , vol.591 , pp. 5823-5831
    • Murphy, R.M.1    Lamb, G.D.2
  • 16
    • 84862169922 scopus 로고    scopus 로고
    • Effects of 2 weeks lower limb immobilization and two separate rehabilitation regimens on gastrocnemius muscle protein turnover signaling and normalization genes
    • Nedergaard A, Jespersen JG, Pingel J, Christensen B, Sroczynski N, Langberg H, Kjaer M, Schjerling P. Effects of 2 weeks lower limb immobilization and two separate rehabilitation regimens on gastrocnemius muscle protein turnover signaling and normalization genes. BMC Res Notes 5: 166, 2012.
    • (2012) BMC Res Notes , vol.5 , pp. 166
    • Nedergaard, A.1    Jespersen, J.G.2    Pingel, J.3    Christensen, B.4    Sroczynski, N.5    Langberg, H.6    Kjaer, M.7    Schjerling, P.8
  • 17
    • 65649104081 scopus 로고    scopus 로고
    • All data are not created equal
    • Neill US. All data are not created equal. J Clin Invest 119: 424, 2009.
    • (2009) J Clin Invest , vol.119 , pp. 424
    • Neill, U.S.1
  • 18
    • 85052410125 scopus 로고    scopus 로고
    • The effect of reduced physical activity and retraining on blood lipids and body composition in young and older adult men
    • in press
    • Nørregaard J, Gram M, Vigelsø A, Wiuff C, Kuhlman AB, Helge JW, Dela F. The effect of reduced physical activity and retraining on blood lipids and body composition in young and older adult men. J Aging Phys Act, in press.
    • J Aging Phys Act
    • Nørregaard, J.1    Gram, M.2    Vigelsø, A.3    Wiuff, C.4    Kuhlman, A.B.5    Helge, J.W.6    Dela, F.7
  • 20
    • 84891689485 scopus 로고    scopus 로고
    • V3 stain-free workflow for a practical, convenient, and reliable total protein loading control in Western blotting
    • Posch A, Kohn J, Oh K, Hammond M, Liu N. V3 stain-free workflow for a practical, convenient, and reliable total protein loading control in Western blotting. J Vis Exp 82: 50948, 2013.
    • (2013) J Vis Exp , vol.82 , pp. 50948
    • Posch, A.1    Kohn, J.2    Oh, K.3    Hammond, M.4    Liu, N.5
  • 23
    • 67650944992 scopus 로고    scopus 로고
    • Multiple reprobing of Western blots after inactivation of peroxidase activity by its substrate, hydrogen peroxide
    • Sennepin AD, Charpentier S, Normand T, Sarre C, Legrand A, Mollet LM. Multiple reprobing of Western blots after inactivation of peroxidase activity by its substrate, hydrogen peroxide. Anal Biochem 393: 129-131, 2009.
    • (2009) Anal Biochem , vol.393 , pp. 129-131
    • Sennepin, A.D.1    Charpentier, S.2    Normand, T.3    Sarre, C.4    Legrand, A.5    Mollet, L.M.6
  • 24
    • 34047207654 scopus 로고    scopus 로고
    • Contraction-mediated glucose uptake is increased in men with impaired glucose tolerance
    • Skov-Jensen C, Skovbro M, Flint A, Helge JW, Dela F. Contraction-mediated glucose uptake is increased in men with impaired glucose tolerance. Appl Physiol Nutr Metab 32: 115-124, 2007.
    • (2007) Appl Physiol Nutr Metab , vol.32 , pp. 115-124
    • Skov-Jensen, C.1    Skovbro, M.2    Flint, A.3    Helge, J.W.4    Dela, F.5
  • 26
    • 84890553910 scopus 로고    scopus 로고
    • A defined methodology for reliable quantification of Western blot data
    • Taylor SC, Berkelman T, Yadav G, Hammond M. A defined methodology for reliable quantification of Western blot data. Mol Biotechnol 55: 217-226, 2013.
    • (2013) Mol Biotechnol , vol.55 , pp. 217-226
    • Taylor, S.C.1    Berkelman, T.2    Yadav, G.3    Hammond, M.4
  • 27
    • 84897530175 scopus 로고    scopus 로고
    • The design of a quantitative Western blot experiment
    • Taylor SC, Posch A. The design of a quantitative Western blot experiment. Biomed Res Int 2014: 361590, 2014.
    • (2014) Biomed Res Int , vol.2014 , pp. 361590
    • Taylor, S.C.1    Posch, A.2
  • 28
    • 0036136997 scopus 로고    scopus 로고
    • Aging of the human neuromuscular system
    • Vandervoort AA. Aging of the human neuromuscular system. Muscle Nerve 25: 17-25, 2002.
    • (2002) Muscle Nerve , vol.25 , pp. 17-25
    • Vandervoort, A.A.1
  • 29
    • 85052409041 scopus 로고    scopus 로고
    • 6 weeks' aerobic retraining after 2 weeks' immobilization restores leg lean mass, and aerobic capacity but does not fully rehabilitate leg strength in young and older men
    • in press
    • Vigelsø A, Gram M, Wiuff C, Andersen JL, Helge JW, Dela F. 6 weeks' aerobic retraining after 2 weeks' immobilization restores leg lean mass, and aerobic capacity but does not fully rehabilitate leg strength in young and older men. J Rehabilitation Med, in press.
    • J Rehabilitation Med
    • Vigelsø, A.1    Gram, M.2    Wiuff, C.3    Andersen, J.L.4    Helge, J.W.5    Dela, F.6
  • 30
    • 84921316662 scopus 로고    scopus 로고
    • Human skeletal muscle perilipin 2 and 3 expression varies with insulin sensitivity
    • Vigelsø A, Prats C, Ploug T, Dela F, Helge JW. Human skeletal muscle perilipin 2 and 3 expression varies with insulin sensitivity. J Biomed Sci Engineer 6: 65-72, 2013.
    • (2013) J Biomed Sci Engineer , vol.6 , pp. 65-72
    • Vigelsø, A.1    Prats, C.2    Ploug, T.3    Dela, F.4    Helge, J.W.5
  • 31
    • 79952400632 scopus 로고    scopus 로고
    • Coomassie staining as loading control in Western blot analysis
    • Welinder C, Ekblad L. Coomassie staining as loading control in Western blot analysis. J Proteome Res 10: 1416-1419, 2011.
    • (2011) J Proteome Res , vol.10 , pp. 1416-1419
    • Welinder, C.1    Ekblad, L.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.