Effects of 2weeks lower limb immobilization and two separate rehabilitation regimens on gastrocnemius muscle protein turnover signaling and normalization genes

BMC Research Notes

Volumn 5, Issue , 2012, Pages

  Nedergaard, Anders ^a,b   Jespersen, Jakob G ^a,b   Pingel, Jessica ^a,b   Christensen, Britt ^a,b   Sroczynski, Nicholas ^a,b   Langberg, Henning ^a,b   Kjaer, Michael ^a,b   Schjerling, Peter ^a,b  

^a BISPEBJERG HOSPITAL   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Akt signaling; Immobilization; Protein supplementation; Rehabilitation; Resistance training

Indexed keywords

MESSENGER RNA; MUSCLE PROTEIN;

ADULT; ARTICLE; CLINICAL TRIAL; GENE EXPRESSION REGULATION; GENETICS; HUMAN; IMMOBILIZATION; LEG; MALE; METABOLISM; PHOSPHORYLATION; PHYSIOLOGY; REHABILITATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; SKELETAL MUSCLE; STANDARD;

GENE EXPRESSION REGULATION; HUMANS; IMMOBILIZATION; LEG; MALE; MUSCLE PROTEINS; MUSCLE, SKELETAL; PHOSPHORYLATION; REFERENCE STANDARDS; REHABILITATION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SIGNAL TRANSDUCTION; YOUNG ADULT;

EID: 84862169922     PISSN: None     EISSN: 17560500     Source Type: Journal    
DOI: 10.1186/1756-0500-5-166     Document Type: Article

References (46)

1. Metabolic responses to reduced daily steps in healthy nonexercising men. Olsen RH, Krogh-Madsen R, Thomsen C, Booth FW, Pedersen BK, JAMA 2008 299 1261 1263 18349087 (Pubitemid 351439093)
2. Myostatin expression during human muscle hypertrophy and subsequent atrophy: increased myostatin with detraining. Jespersen JG, Nedergaard A, Andersen LL, Schjerling P, Andersen JL, Scand J Med Sci Sports 2011 21 215 23 10.1111/j.1600-0838.2009.01044.x 19903317
3. Alterations of protein turnover underlying disuse atrophy in human skeletal muscle. Phillips SM, Glover EI, Rennie MJ, J Appl Physiol 2009 107 645 654 19608931
4. Epidemiology of sarcopenia among the elderly in New Mexico. Baumgartner RN, Koehler KM, Gallagher D, Romero L, Heymsfield SB, Ross RR, Garry PJ, Lindeman RD, Am J Epidemiol 1998 147 755 763 10.1093/oxfordjournals.aje.a009520 9554417 (Pubitemid 28183275)
5. Cachexia: a new definition. Evans WJ, Morley JE, Argiles J, Bales C, Baracos V, Guttridge D, Jatoi A, Kalantar-Zadeh K, Lochs H, Mantovani G, et al. Clin Nutr 2008 27 793 799 10.1016/j.clnu.2008.06.013 18718696
6. The temporal responses of protein synthesis, gene expression and cell signalling in human quadriceps muscle and patellar tendon to disuse. de Boer MD, Selby A, Atherton P, Smith K, Seynnes OR, Maganaris CN, Maffulli N, Movin T, Narici MV, Rennie MJ, J Physiol 2007 585 241 251 10.1113/jphysiol.2007.142828 17901116 (Pubitemid 350131116)
7. Disuse atrophy and exercise rehabilitation in humans profoundly affects the expression of genes associated with the regulation of skeletal muscle mass. Jones SW, Hill RJ, Krasney PA, OConner B, Peirce N, Greenhaff PL, FASEB J 2004 18 1025 1027 15084522 (Pubitemid 39561513)
8. Immobilization induces anabolic resistance in human myofibrillar protein synthesis with low and high dose amino acid infusion. Glover EI, Phillips SM, Oates BR, Tang JE, Tarnopolsky MA, Selby A, Smith K, Rennie MJ, J Physiol 2008 586 6049 6061 10.1113/jphysiol.2008.160333 18955382
9. Effects of aging on muscle mechanical function and muscle fiber morphology during short-term immobilization and subsequent retraining. Hvid L, Aagaard P, Justesen L, Bayer ML, Andersen JL, Ortenblad N, Kjaer M, Suetta C, J Appl Physiol 2010 109 1628 1634 10.1152/japplphysiol.00637.2010 20864557
10. Leucine-enriched essential amino acid and carbohydrate ingestion following resistance exercise enhances mTOR signaling and protein synthesis in human muscle. Dreyer HC, Drummond MJ, Pennings B, Fujita S, Glynn EL, Chinkes DL, Dhanani S, Volpi E, Rasmussen BB, Am J Physiol Endocrinol Metab 2008 294 392 E400 18056791
11. Nutrient signalling in the regulation of human muscle protein synthesis. Fujita S, Dreyer HC, Drummond MJ, Glynn EL, Cadenas JG, Yoshizawa F, Volpi E, Rasmussen BB, J Physiol 2007 582 813 823 10.1113/jphysiol.2007.134593 17478528 (Pubitemid 47040563)
12. Essential amino acid and carbohydrate supplementation ameliorates muscle protein loss in humans during 28days bedrest. Paddon-Jones D, Sheffield-Moore M, Urban RJ, Sanford AP, Aarsland A, Wolfe RR, Ferrando AA, J Clin Endocrinol Metab 2004 89 4351 4358 10.1210/jc.2003-032159 15356032 (Pubitemid 39244437)
13. Influence of concurrent exercise or nutrition countermeasures on thigh and calf muscle size and function during 60days of bed rest in women. Trappe TA, Burd NA, Louis ES, Lee GA, Trappe SW, Acta Physiol (Oxf) 2007 191 147 159 10.1111/j.1748-1716.2007.01728.x (Pubitemid 47390302)
14. Mechanisms regulating muscle mass during disuse atrophy and rehabilitation in humans. Marimuthu K, Murton AJ, Greenhaff PL, J Appl Physiol 2011 110 555 560 10.1152/japplphysiol.00962.2010 21030670
15. Physiological control of muscle mass in humans during resistance exercise, disuse and rehabilitation. Murton AJ, Greenhaff PL, Curr Opin Clin Nutr Metab Care 2010 13 249 254 10.1097/MCO.0b013e3283374d19 20110809
16. Signaling in muscle atrophy and hypertrophy. Sandri M, Physiology (Bethesda) 2008 23 160 170 10.1152/physiol.00041.2007
17. Molecular mechanisms and treatment options for muscle wasting diseases. Ruegg MA, Glass DJ, Annu Rev Pharmacol Toxicol 2011 51 373 395 10.1146/annurev-pharmtox-010510-100537 20936944
18. Identification of ubiquitin ligases required for skeletal muscle atrophy. Bodine SC, Latres E, Baumhueter S, Lai VK, Nunez L, Clarke BA, Poueymirou WT, Panaro FJ, Dharmarajan K, et al. Science 2001 294 1704 1708 10.1126/science. 1065874 11679633 (Pubitemid 33104839)
19. Akt/mTOR pathway is a crucial regulator of skeletal muscle hypertrophy and can prevent muscle atrophy in vivo. Bodine SC, Stitt TN, Gonzalez M, Kline WO, Stover GL, Bauerlein R, Zlotchenko E, Scrimgeour A, Lawrence JC, Glass DJ, Yancopoulos GD, Nat Cell Biol 2001 3 1014 1019 10.1038/ncb1101-1014 11715023 (Pubitemid 34428747)
20. FoxO3 coordinately activates protein degradation by the autophagic/lysosomal and proteasomal pathways in atrophying muscle cells. Zhao J, Brault JJ, Schild A, Cao P, Sandri M, Schiaffino S, Lecker SH, Goldberg AL, Cell Metab 2007 6 472 483 10.1016/j.cmet.2007.11.004 18054316 (Pubitemid 350163056)
21. Mediation of IGF-1-induced skeletal myotube hypertrophy by PI(3)K/Akt/mTOR and PI(3)K/Akt/GSK3 pathways. Rommel C, Bodine SC, Clarke BA, Rossman R, Nunez L, Stitt TN, Yancopoulos GD, Glass DJ, Nat Cell Biol 2001 3 1009 1013 10.1038/ncb1101-1009 11715022 (Pubitemid 34428746)
22. Conditional activation of akt in adult skeletal muscle induces rapid hypertrophy. Lai KM, Gonzalez M, Poueymirou WT, Kline WO, Na E, Zlotchenko E, Stitt TN, Economides AN, Yancopoulos GD, Glass DJ, Mol Cell Biol 2004 24 9295 9304 10.1128/MCB.24.21.9295-9304.2004 15485899 (Pubitemid 39391668)
23. Foxo transcription factors induce the atrophy-related ubiquitin ligase atrogin-1 and cause skeletal muscle atrophy. Sandri M, Sandri C, Gilbert A, Skurk C, Calabria E, Picard A, Walsh K, Schiaffino S, Lecker SH, Goldberg AL, Cell 2004 117 399 412 10.1016/S0092-8674(04)00400-3 15109499 (Pubitemid 38534545)
24. Rapid disuse and denervation atrophy involve transcriptional changes similar to those of muscle wasting during systemic diseases. Sacheck JM, Hyatt JP, Raffaello A, Jagoe RT, Roy RR, Edgerton VR, Lecker SH, Goldberg AL, FASEB J 2007 21 140 155 17116744 (Pubitemid 46052553)
25. Multiple types of skeletal muscle atrophy involve a common program of changes in gene expression. Lecker SH, Jagoe RT, Gilbert A, Gomes M, Baracos V, Bailey J, Price SR, Mitch WE, Goldberg AL, FASEB J 2004 18 39 51 10.1096/fj.03-0610com 14718385 (Pubitemid 38076464)
26. Short-term immobilization and recovery affect skeletal muscle but not collagen tissue turnover in humans. Christensen B, Dyrberg E, Aagaard P, Kjaer M, Langberg H, J Appl Physiol 2008 105 1845 1851 10.1152/japplphysiol.90445.2008 18927270
27. Effect of estrogen on tendon collagen synthesis, tendon structural characteristics, and biomechanical properties in postmenopausal women. Hansen M, Kongsgaard M, Holm L, Skovgaard D, Magnusson SP, Qvortrup K, Larsen JO, Aagaard P, Dahl M, Serup A, et al. J Appl Physiol 2009 106 1385 1393 10.1152/japplphysiol.90935.2008 18927264
28. The influence of training status on the drop in muscle strength after acute exercise. Pingel J, Moerch L, Kjaer M, Langberg H, Eur J Appl Physiol 2009 106 605 611 10.1007/s00421-009-1055-0 19363682
29. Load-displacement properties of the human triceps surae aponeurosis in vivo. Magnusson SP, Aagaard P, Dyhre-Poulsen P, Kjaer M, J Physiol 2001 531 277 288 10.1111/j.1469-7793.2001.0277j.x 11179410 (Pubitemid 32178044)
30. Percutaneous needle biopsy of skeletal muscle in physiological and clinical research. Bergstrom J, Scand J Clin Lab Invest 1975 35 609 616 10.3109/00365517509095787 1108172
31. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Chomczynski P, Sacchi N, Anal Biochem 1987 162 156 159 2440339 (Pubitemid 17064313)
32. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F, Genome Biol 2002 3 ESEARCH0034 12184808
33. Activated protein synthesis and suppressed protein breakdown signaling in skeletal muscle of critically ill patients. Jespersen JG, Nedergaard A, Reitelseder S, Mikkelsen UR, Dideriksen KJ, Agergaard J, Kreiner F, Pott FC, Schjerling P, Kjaer M, PLoS One 2011 6 18090 10.1371/journal.pone.0018090 21483870
34. The adaptive responses in several mediators linked with hypertrophy and atrophy of skeletal muscle after lower limb unloading in humans. Sakuma K, Watanabe K, Hotta N, Koike T, Ishida K, Katayama K, Akima H, Acta Physiol (Oxf) 2009 197 151 159 10.1111/j.1748-1716.2009.01995.x
35. FoxO3 controls autophagy in skeletal muscle in vivo. Mammucari C, Milan G, Romanello V, Masiero E, Rudolf R, Del Piccolo P, Burden SJ, Di Lisi R, Sandri C, Zhao J, et al. Cell Metab 2007 6 458 471 10.1016/j.cmet.2007.11.001 18054315 (Pubitemid 350163055)
36. FoxO1 induces apoptosis in skeletal myotubes in a DNA-binding-dependent manner. McLoughlin TJ, Smith SM, DeLong AD, Wang H, Unterman TG, Esser KA, Am J Physiol Cell Physiol 2009 297 548 C555 10.1152/ajpcell.00502.2008 19553561
37. TNF induction of atrogin-1/MAFbx mRNA depends on Foxo4 expression but not AKT-Foxo1/3 signaling. Moylan JS, Smith JD, Chambers MA, McLoughlin TJ, Reid MB, Am J Physiol Cell Physiol 2008 295 986 C993 10.1152/ajpcell.00041.2008 18701653
38. Ros-mediated activation of NF-kappaB and Foxo during muscle disuse. Dodd SL, Gagnon BJ, Senf SM, Hain BA, Judge AR, Muscle Nerve 2010 41 110 113 10.1002/mus.21526 19813194
39. Effects of 3days unloading on molecular regulators of muscle size in humans. Gustafsson T, Osterlund T, Flanagan JN, von Walden F, Trappe TA, Linnehan RM, Tesch PA, J Appl Physiol 2010 109 721 727 20538844
40. Limb immobilization induces a coordinate down-regulation of mitochondrial and other metabolic pathways in men and women. Abadi A, Glover EI, Isfort RJ, Raha S, Safdar A, Yasuda N, Kaczor JJ, Melov S, Hubbard A, Qu X, et al. PLoS One 2009 4 6518 10.1371/journal.pone.0006518 19654872
41. Effect of unloading followed by reloading on expression of collagen and related growth factors in rat tendon and muscle. Heinemeier KM, Olesen JL, Haddad F, Schjerling P, Baldwin KM, Kjaer M, J Appl Physiol 2009 106 178 186 18988763
42. Analysis of human skeletal muscle after 48h immobilization reveals alterations in mRNA and protein for extracellular matrix components. Urso ML, Scrimgeour AG, Chen YW, Thompson PD, Clarkson PM, J Appl Physiol 2006 101 1136 1148 10.1152/japplphysiol.00180.2006 16763108 (Pubitemid 44497558)
43. Molecular properties and gene expression of albumin in the skeletal muscle following hindlimb immobilization in a shortened position. Wagatsuma A, Yamazaki Y, Mizuno K, Yamada S, Acta Neuropathol 2001 101 540 546 11515781 (Pubitemid 32684751)
44. Changes in muscle strength, muscle fibre size and myofibrillar gene expression after immobilization and retraining in humans. Hortobagyi T, Dempsey L, Fraser D, Zheng D, Hamilton G, Lambert J, Dohm L, J Physiol 2000 524 Pt 1 293 304 10747199 (Pubitemid 30195321)
45. Experimental changes to limb muscles elicit contralateral reactions: the problem of controls. Lu D-X, Käser L, Muntener M, J Exp Biol 1999 202 1691 1700 10333514 (Pubitemid 29321702)
46. Control of the size of the human muscle mass. Rennie MJ, Wackerhage H, Spangenburg EE, Booth FW, Annu Rev Physiol 2004 66 799 828 10.1146/annurev. physiol.66.052102.134444 14977422 (Pubitemid 40614463)