Uncovering suitable reference proteins for expression studies in human adipose tissue with relevance to obesity

PLoS ONE

Volumn 7, Issue 1, 2012, Pages

  Pérez Pérez, Rafael ^a,b   López, Juan A ^c   García Santos, Eva ^a,b   Camafeita, Emilio ^c   Gómez Serrano, María ^a,b   Ortega Delgado, Francisco J ^b,d   Ricart, Wifredo ^b,d   Fernández Real, José M ^b,d   Peral, Belén ^a,b  

^a UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

^b INSTITUTO DE SALUD CARLOS III   (Spain)

^c CENTRO NACIONAL DE INVESTIGACIONES CARDIOVASCULARES CNIC   (Spain)

^d HOSPITAL JOSEP TRUETA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYL COENZYME A ACETYLTRANSFERASE; ADENOSYLHOMOCYSTEINASE; ALPHA ENOLASE; BETA ACTIN; CRYSTALLIN ALPHA B; DJ 1 PROTEIN; ENOLASE 1; ENOYL COA HIDRATASE; EUKARYOTIC TRANSLATION ELONGATION FACTOR 1 GAMMA; F ACTIN CAPPING PROTEIN ALPHA 1 SUBUNIT; FUMARYLACETOACETASE; GALECTIN 1; GDP DISSOCIATION INHIBITOR 2; NADP DEPENDENT ISOCITRATE DEHYDROGENASE; PARKINSON DISEASE PROTEIN 7; PEROXIREDOXIN 6; PHOSPHOGLYCERATE KINASE 1; PROTEASOME 26S NON ATPASE SUBUNIT 7; PROTEIN DISULFIDE ISOMERASE; PROTEIN DISULFIDE ISOMERASE FAMILY A MEMBER 3; TRANSALDOLASE 1; UNCLASSIFIED DRUG; VESICLE AMINE TRANSPORT PROTEIN 1; WDR1 PROTEIN;

ADIPOSE TISSUE; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; FEMALE; FLUORESCENCE; HUMAN; HUMAN TISSUE; IMMUNOBLOTTING; MALE; MASS SPECTROMETRY; NUCLEOTIDE SEQUENCE; OBESITY; PERITONEAL FAT; PROTEIN ANALYSIS; PROTEIN BLOOD LEVEL; PROTEIN EXPRESSION; PROTEIN STABILITY; STANDARDIZATION; SUBCUTANEOUS FAT; TWO DIMENSIONAL DIFFERENCE GEL ELECTROPHORESIS; VALIDATION PROCESS; WESTERN BLOTTING;

EID: 84889697829     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0030326     Document Type: Article

References (38)

1. Aldridge GM, Podrebarac DM, Greenough WT, Weiler IJ, (2008) The use of total protein stains as loading controls: an alternative to high-abundance single-protein controls in semi-quantitative immunoblotting. J Neurosci Methods 172: 250-254.
2. Ferguson RE, Carroll HP, Harris A, Maher ER, Selby PJ, et al. (2005) Housekeeping proteins: a preliminary study illustrating some limitations as useful references in protein expression studies. Proteomics 5: 566-571.
3. Dittmer A, Dittmer J, (2006) Beta-actin is not a reliable loading control in Western blot analysis. Electrophoresis 27: 2844-2845.
4. Spiegelman BM, Flier JS, (2001) Obesity and the regulation of energy balance. Cell 104: 531-543.
5. Fox CS, Massaro JM, Hoffmann U, Pou KM, Maurovich-Horvat P, et al. (2007) Abdominal visceral and subcutaneous adipose tissue compartments: association with metabolic risk factors in the Framingham Heart Study. Circulation 116: 39-48.
6. Matsuzawa Y, (2006) Therapy Insight: adipocytokines in metabolic syndrome and related cardiovascular disease. Nat Clin Pract Cardiovasc Med 3: 35-42.
7. Prokesch A, Hackl H, Hakim-Weber R, Bornstein SR, Trajanoski Z, (2009) Novel insights into adipogenesis from omics data. Curr Med Chem 16: 2952-2964.
8. Rasche A, Al-Hasani H, Herwig R, (2008) Meta-analysis approach identifies candidate genes and associated molecular networks for type-2 diabetes mellitus. BMC Genomics 9: 310.
9. del Pozo CH, Calvo RM, Vesperinas-Garcia G, Gomez-Ambrosi J, Fruhbeck G, et al. (2011) Expression profile in omental and subcutaneous adipose tissue from lean and obese subjects. Repression of lipolytic and lipogenic genes. Obes Surg 21: 633-643.
10. Perez-Perez R, Ortega-Delgado FJ, Garcia-Santos E, Lopez JA, Camafeita E, et al. (2009) Differential proteomics of omental and subcutaneous adipose tissue reflects their unalike biochemical and metabolic properties. J Proteome Res 8: 1682-1693.
11. Vohl MC, Sladek R, Robitaille J, Gurd S, Marceau P, et al. (2004) A survey of genes differentially expressed in subcutaneous and visceral adipose tissue in men. Obes Res 12: 1217-1222.
12. Gesta S, Bluher M, Yamamoto Y, Norris AW, Berndt J, et al. (2006) Evidence for a role of developmental genes in the origin of obesity and body fat distribution. Proc Natl Acad Sci U S A 103: 6676-6681.
13. van Beek EA, Bakker AH, Kruyt PM, Hofker MH, Saris WH, et al. (2007) Intra- and interindividual variation in gene expression in human adipose tissue. Pflugers Arch 453: 851-861.
14. MacLaren R, Cui W, Simard S, Cianflone K, (2008) Influence of obesity and insulin sensitivity on insulin signaling genes in human omental and subcutaneous adipose tissue. J Lipid Res 49: 308-323.
15. Peinado JR, Jimenez-Gomez Y, Pulido MR, Ortega-Bellido M, Diaz-Lopez C, et al. (2010) The stromal-vascular fraction of adipose tissue contributes to major differences between subcutaneous and visceral fat depots. Proteomics 10: 3356-3366.
16. Hurtado del Pozo C, Calvo RM, Vesperinas-Garcia G, Gomez-Ambrosi J, Fruhbeck G, et al. (2010) IPO8 and FBXL10: new reference genes for gene expression studies in human adipose tissue. Obesity (Silver Spring) 18: 897-903.
17. Catalan V, Gomez-Ambrosi J, Rotellar F, Silva C, Rodriguez A, et al. (2007) Validation of endogenous control genes in human adipose tissue: relevance to obesity and obesity-associated type 2 diabetes mellitus. Horm Metab Res 39: 495-500.
18. Gabrielsson BG, Olofsson LE, Sjogren A, Jernas M, Elander A, et al. (2005) Evaluation of reference genes for studies of gene expression in human adipose tissue. Obes Res 13: 649-652.
19. Pérez-Pérez R, García-Santos E, Ortega-Delgado FJ, López JA, Camafeita E, et al. (2011) Attenuated metabolism is a hallmark of obesity as revealed by comparative proteomic analysis of human omental adipose tissue. Journal of Proteomics doi:10.1016/j.jprot.2011.09.016.
20. Suckau D, Resemann A, Schuerenberg M, Hufnagel P, Franzen J, et al. (2003) A novel MALDI LIFT-TOF/TOF mass spectrometer for proteomics. Anal Bioanal Chem 376: 952-965.
21. Perkins DN, Pappin DJ, Creasy DM, Cottrell JS, (1999) Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis 20: 3551-3567.
22. Sun S, Yi X, Poon RT, Yeung C, Day PJ, et al. (2009) A protein-based set of reference markers for liver tissues and hepatocellular carcinoma. BMC Cancer 9: 309.
23. Wu X, Hoffstedt J, Deeb W, Singh R, Sedkova N, et al. (2001) Depot-specific variation in protein-tyrosine phosphatase activities in human omental and subcutaneous adipose tissue: a potential contribution to differential insulin sensitivity. J Clin Endocrinol Metab 86: 5973-5980.
24. Gregor MF, Yang L, Fabbrini E, Mohammed BS, Eagon JC, et al. (2009) Endoplasmic reticulum stress is reduced in tissues of obese subjects after weight loss. Diabetes 58: 693-700.
25. Boden G, Duan X, Homko C, Molina EJ, Song W, et al. (2008) Increase in endoplasmic reticulum stress-related proteins and genes in adipose tissue of obese, insulin-resistant individuals. Diabetes 57: 2438-2444.
26. Sharma NK, Das SK, Mondal AK, Hackney OG, Chu WS, et al. (2008) Endoplasmic reticulum stress markers are associated with obesity in nondiabetic subjects. J Clin Endocrinol Metab 93: 4532-4541.
27. Mantovani G, Bondioni S, Alberti L, Gilardini L, Invitti C, et al. (2009) Protein kinase A regulatory subunits in human adipose tissue: decreased R2B expression and activity in adipocytes from obese subjects. Diabetes 58: 620-626.
28. Bashan N, Dorfman K, Tarnovscki T, Harman-Boehm I, Liberty IF, et al. (2007) Mitogen-activated protein kinases, inhibitory-kappaB kinase, and insulin signaling in human omental versus subcutaneous adipose tissue in obesity. Endocrinology 148: 2955-2962.
29. Kovsan J, Bluher M, Tarnovscki T, Kloting N, Kirshtein B, et al. (2011) Altered autophagy in human adipose tissues in obesity. J Clin Endocrinol Metab 96: E268-277.
30. Despres JP, Lemieux I, (2006) Abdominal obesity and metabolic syndrome. Nature 444: 881-887.
31. van Hinsbergh VW, Kooistra T, Scheffer MA, Hajo van Bockel J, van Muijen GN, (1990) Characterization and fibrinolytic properties of human omental tissue mesothelial cells. Comparison with endothelial cells. Blood 75: 1490-1497.
32. Kim RH, Peters M, Jang Y, Shi W, Pintilie M, et al. (2005) DJ-1, a novel regulator of the tumor suppressor PTEN. Cancer Cell 7: 263-273.
33. Kahle PJ, Waak J, Gasser T, (2009) DJ-1 and prevention of oxidative stress in Parkinson's disease and other age-related disorders. Free Radic Biol Med 47: 1354-1361.
34. Rai P, Shivaji S, (2011) The role of DJ-1 in the pathogenesis of endometriosis. PLoS One 6: e18074.
35. Liu S, Yang Z, Wei H, Shen W, Liu J, et al. (2010) Increased DJ-1 and its prognostic significance in hepatocellular carcinoma. Hepatogastroenterology 57: 1247-1256.
36. McNally RS, Davis BK, Clements CM, Accavitti-Loper MA, Mak TW, et al. (2011) DJ-1 enhances cell survival through the binding of Cezanne, a negative regulator of NF-kappaB. J Biol Chem 286: 4098-4106.
37. Zhang L, Shimoji M, Thomas B, Moore DJ, Yu SW, et al. (2005) Mitochondrial localization of the Parkinson's disease related protein DJ-1: implications for pathogenesis. Hum Mol Genet 14: 2063-2073.
38. Tanguay RM, Valet JP, Lescault A, Duband JL, Laberge C, et al. (1990) Different molecular basis for fumarylacetoacetate hydrolase deficiency in the two clinical forms of hereditary tyrosinemia (type I). Am J Hum Genet 47: 308-316.