Coomassie staining as loading control in Western blot analysis

Journal of Proteome Research

Volumn 10, Issue 3, 2011, Pages 1416-1419

  Welinder, Charlotte ^a   Ekblad, Lars ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

Coomassie; GAPDH; loading control; MALDITOF; peptide mass fingerprinting; PVDF; Western blot

Indexed keywords

ANAZOLENE SODIUM;

ANTIBODY DETECTION; ARTICLE; CONTROLLED STUDY; IMMUNODETECTION; IMMUNOHISTOCHEMISTRY; INTERMETHOD COMPARISON; MASS SPECTROMETRY; PRIORITY JOURNAL; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; PEPTIDE MAPPING; PROTEINS; ROSANILINE DYES; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STAINING AND LABELING;

EID: 79952400632     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr1011476     Document Type: Article

References (12)

1. Dittmer, A.; Dittmer, J. Beta-actin is not a reliable loading control in Western blot analysis Electrophoresis 2006, 27 (14) 2844-5 (Pubitemid 44160833)
2. Yamatodani, T.; Ekblad, L.; Kjellén, E.; Johnsson, A.; Mineta, H.; Wennerberg, J. Epidermal growth factor receptor status and persistent activation of Akt and p44/42 MAPK pathways correlate with the effect of cetuximab in head and neck and colon cancer cell lines J. Cancer Res. Clin. Oncol. 2009, 135 (3) 395-402
3. Welinder, C.; Jansson, B.; Fernö, M.; Olsson, H.; Baldetorp, B. Expression of Helix pomatia lectin binding glycoproteins in women with breast cancer in relationship to their blood group phenotypes J. Proteome Res. 2009, 8 (2) 782-7
4. Matsudaira, P. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes J. Biol. Chem. 1987, 262 (21) 10035-8
5. Lin, Y.; Li, Y.; Liu, Y.; Han, W.; He, Q.; Li, J.; Chen, P.; Wang, X.; Liang, S. Improvement of gel-separated protein identification by DMF-assisted digestion and peptide recovery after electroblotting Electrophoresis 2009, 30 (20) 3626-35
6. Luque-Garcia, J. L.; Zhou, G.; Spellman, D. S.; Sun, T. T.; Neubert, T. A. Analysis of electroblotted proteins by mass spectrometry: protein identification after Western blotting Mol. Cell. Proteomics 2008, 7 (2) 308-14 (Pubitemid 351298445)
7. Methogo, R. M.; Dufresne-Martin, G.; Leclerc, P.; Leduc, R.; Klarskov, K. Mass spectrometric peptide fingerprinting of proteins after Western blotting on polyvinylidene fluoride and enhanced chemiluminescence detection J. Proteome Res. 2005, 4 (6) 2216-24 (Pubitemid 41814290)
8. Klein, D.; Kern, R. M.; Sokol, R. Z. A method for quantification and correction of proteins after transfer to immobilization membranes Biochem. Mol. Biol. Int. 1995, 36 (1) 59-66 (Pubitemid 26172956)
9. Romero-Calvo, I.; Ocon, B.; Martinez-Moya, P.; Suarez, M. D.; Zarzuelo, A.; Martinez-Augustin, O.; de Medina, F. S. Reversible Ponceau staining as a loading control alternative to actin in Western blots Anal. Biochem. 2010, 401 (2) 318-20
10. DSouza, A.; Scofield, R. H. Protein stains to detect antigen on membranes Methods Mol. Biol. 2009, 536, 433-40
11. Gallagher, S.; Winston, S. E.; Fuller, S. A.; Hurrell, J. G. Immunoblotting and Immunodetection. Current Protocols in Immunology; 2008; 83, 8.10.1-8.10.28.
12. Pryor, J. L.; Xu, W.; Hamilton, D. W. Immunodetection after complete destaining of coomassie blue-stained proteins on immobilon-PVDF Anal. Biochem. 1992, 202 (1) 100-4