Reversible Ponceau staining as a loading control alternative to actin in Western blots

Analytical Biochemistry

Volumn 401, Issue 2, 2010, Pages 318-320

  Romero Calvo, Isabel ^a   Ocón, Borja ^a   Martínez Moya, Patricia ^a   Suárez, María Dolores ^a   Zarzuelo, Antonio ^a   Martínez Augustin, Olga ^a   de Medina, Fermín Sánchez ^a  

^a UNIVERSITY OF GRANADA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

DENSITOMETRIC ANALYSIS; HOUSEKEEPING GENE; LOADING CONTROL; STANDARD PRACTICES; WESTERN BLOTS;

PROTEINS;

ACTIN; DYE; PONCEAU S; UNCLASSIFIED DRUG;

ARTICLE; CENTRIFUGATION; DEGRADATION; DENSITOMETRY; DIMERIZATION; ELECTROPHORESIS; GLYCOSYLATION; INCUBATION TIME; LOADING TEST; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONTENT; STAINING; VALIDATION PROCESS; WESTERN BLOTTING;

EID: 77952865658     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.02.036     Document Type: Article

References (5)

1. Smith P.K., Krohn R.I., Hermanson G.T., Mallia A.K., Gartner F.H., Provenzano M.D., Fujimoto E.K., Goeke N.M., Olson B.J., Klenk D.C. Measurement of protein using bicinchoninic acid. Anal. Biochem. 1985, 150:76-85.
2. Lin J.J. Monoclonal antibodies against myofibrillar components of rat skeletal muscle decorate the intermediate filaments of cultured cells. Proc. Natl. Acad. Sci. USA 1981, 78:2335-2339.
3. Nakamura K., Tanaka T., Kuwahara A., Takeo K. Microassay for proteins on nitrocellulose filter using protein dye-staining procedure. Anal. Biochem. 1985, 148:311-319.
4. Aldridge G.M., Podrebarac D.M., Greenough W.T., Weiler I.J. The use of total protein stains as loading controls: an alternative to high-abundance single-protein controls in semi-quantitative immunoblotting. J. Neurosci. Methods 2008, 172:250-254.
5. Dittmer A., Dittmer J. β-Actin is not a reliable loading control in Western blot analysis. Electrophoresis 2006, 27:2844-2845.