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Volumn 2014, Issue , 2014, Pages

The design of a quantitative western blot experiment

Author keywords

[No Author keywords available]

Indexed keywords

CALCULATION; CHEMOLUMINESCENCE; DATA ANALYSIS; DENSITOMETRY; EXPERIMENTAL DESIGN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN TARGETING; QUANTITATIVE ANALYSIS; RELIABILITY; REPRODUCIBILITY; REVIEW; WESTERN BLOTTING; CHEMISTRY; LUMINESCENCE; PROCEDURES; STATISTICS;

EID: 84897530175     PISSN: 23146133     EISSN: 23146141     Source Type: Journal    
DOI: 10.1155/2014/361590     Document Type: Review
Times cited : (281)

References (27)
  • 1
    • 65549097797 scopus 로고    scopus 로고
    • Two-dimensional gel electrophoresis and mass spectrometry for biomarker discovery
    • 2-s2.0-65549097797 10.1002/prca.200800025
    • Penque D., Two-dimensional gel electrophoresis and mass spectrometry for biomarker discovery. Proteomics-Clinical Applications 2009 3 2 155 172 2-s2.0-65549097797 10.1002/prca.200800025
    • (2009) Proteomics - Clinical Applications , vol.3 , Issue.2 , pp. 155-172
    • Penque, D.1
  • 2
    • 0009482260 scopus 로고
    • Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
    • Towbin H., Staehelin T., Gordon J., Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 1979 76 9 4350 4354 2-s2.0-0009482260 (Pubitemid 9256559)
    • (1979) Proceedings of the National Academy of Sciences of the United States of America , vol.76 , Issue.9 , pp. 4350-4354
    • Towbin, H.1    Staehelin, T.2    Gordon, J.3
  • 3
    • 0037017766 scopus 로고    scopus 로고
    • Sensitive fluorescent detection of protein on nylon membranes
    • DOI 10.1016/S0165-022X(01)00243-3, PII S0165022X01002433
    • Dubitsky A., DeCollibus D., Ortolano G. A., Sensitive fluorescent detection of protein on nylon membranes. Journal of Biochemical and Biophysical Methods 2002 51 1 47 56 2-s2.0-0037017766 10.1016/S0165-022X(01)00243-3 (Pubitemid 34203811)
    • (2002) Journal of Biochemical and Biophysical Methods , vol.51 , Issue.1 , pp. 47-56
    • Dubitsky, A.1    DeCollibus, D.2    Ortolano, G.A.3
  • 4
    • 84897560336 scopus 로고    scopus 로고
    • Increase Western blot throughput with multiplex fluorescent detection: Advice and tips on how to quickly generate reliable and reproducible results
    • McDonald K., Increase Western blot throughput with multiplex fluorescent detection: advice and tips on how to quickly generate reliable and reproducible results. Pharma 2010
    • (2010) Pharma
    • McDonald, K.1
  • 6
    • 59749100523 scopus 로고    scopus 로고
    • Are genuine changes in protein expression being overlooked? Reassessing Western blotting
    • 2-s2.0-59749100523 10.1016/j.ab.2008.12.029
    • Mollica J. P., Oakhill J. S., Lamb G. D., Murphy R. M., Are genuine changes in protein expression being overlooked? Reassessing Western blotting. Analytical Biochemistry 2009 386 2 270 275 2-s2.0-59749100523 10.1016/j.ab.2008.12.029
    • (2009) Analytical Biochemistry , vol.386 , Issue.2 , pp. 270-275
    • Mollica, J.P.1    Oakhill, J.S.2    Lamb, G.D.3    Murphy, R.M.4
  • 7
    • 33645293489 scopus 로고    scopus 로고
    • A picture worth a thousand words (of explanation)
    • Editorial, A picture worth a thousand words (of explanation). Nature Methods 2006 3 4 237
    • (2006) Nature Methods , vol.3 , Issue.4 , pp. 237
    • Editorial1
  • 8
    • 84890553910 scopus 로고    scopus 로고
    • A defined methodology for reliable quantification of Western blot data
    • Taylor S. C., Berkelman T., Yadav G., Hammond M., A defined methodology for reliable quantification of Western blot data. Molecular Biotechnology 2013 55 3 217 226
    • (2013) Molecular Biotechnology , vol.55 , Issue.3 , pp. 217-226
    • Taylor, S.C.1    Berkelman, T.2    Yadav, G.3    Hammond, M.4
  • 9
    • 84897532701 scopus 로고    scopus 로고
    • A Method for Greater Reliability in Western Blot Loading Controls - Stain-Free Total Protein Quantitation
    • Hammond M., Kohn J., Oh K., Piatti P., Liu N., A Method for Greater Reliability in Western Blot Loading Controls-Stain-Free Total Protein Quantitation. Bio-Rad Bulletin: 6360, 2013
    • (2013) Bio-Rad Bulletin , vol.6360
    • Hammond, M.1    Kohn, J.2    Oh, K.3    Piatti, P.4    Liu, N.5
  • 10
    • 33746738785 scopus 로고    scopus 로고
    • β-Actin is not a reliable loading control in Western blot analysis
    • DOI 10.1002/elps.200500785
    • Dittmer A., Dittmer J., β -Actin is not a reliable loading control in Western blot analysis. Electrophoresis 2006 27 14 2844 2845 2-s2.0-33746738785 10.1002/elps.200500785 (Pubitemid 44160833)
    • (2006) Electrophoresis , vol.27 , Issue.14 , pp. 2844-2845
    • Dittmer, A.1    Dittmer, J.2
  • 11
    • 46249108210 scopus 로고    scopus 로고
    • The use of total protein stains as loading controls: An alternative to high-abundance single-protein controls in semi-quantitative immunoblotting
    • 2-s2.0-46249108210 10.1016/j.jneumeth.2008.05.003
    • Aldridge G. M., Podrebarac D. M., Greenough W. T., Weiler I. J., The use of total protein stains as loading controls: an alternative to high-abundance single-protein controls in semi-quantitative immunoblotting. Journal of Neuroscience Methods 2008 172 2 250 254 2-s2.0-46249108210 10.1016/j.jneumeth. 2008.05.003
    • (2008) Journal of Neuroscience Methods , vol.172 , Issue.2 , pp. 250-254
    • Aldridge, G.M.1    Podrebarac, D.M.2    Greenough, W.T.3    Weiler, I.J.4
  • 12
    • 84865155360 scopus 로고    scopus 로고
    • Avoiding pitfalls of internal controls: Validation of reference genes for analysis by qRT-PCR and western blot throughout rat retinal development
    • 43028
    • Rocha-Martins M., Njaine B., Silveira M. S., Avoiding pitfalls of internal controls: validation of reference genes for analysis by qRT-PCR and western blot throughout rat retinal development. PLoS One 2012 7 8 43028
    • (2012) PLoS One , vol.7 , Issue.8
    • Rocha-Martins, M.1    Njaine, B.2    Silveira, M.S.3
  • 13
    • 84856960677 scopus 로고    scopus 로고
    • Stability of reference proteins in human placenta: General protein stains are the benchmark
    • 2-s2.0-84856960677 10.1016/j.placenta.2011.12.008
    • Lanoix D., St-Pierre J., Lacasse A.-A., Viau M., Lafond J., Vaillancourt C., Stability of reference proteins in human placenta: general protein stains are the benchmark. Placenta 2012 33 3 151 156 2-s2.0-84856960677 10.1016/j.placenta.2011.12.008
    • (2012) Placenta , vol.33 , Issue.3 , pp. 151-156
    • Lanoix, D.1    St-Pierre, J.2    Lacasse, A.-A.3    Viau, M.4    Lafond, J.5    Vaillancourt, C.6
  • 14
    • 77951206695 scopus 로고    scopus 로고
    • Housekeeping genes; Expression levels may change with density of cultured cells
    • 2-s2.0-77951206695 10.1016/j.jim.2010.02.006
    • Greer S., Honeywell R., Geletu M., Arulanandam R., Raptis L., Housekeeping genes; expression levels may change with density of cultured cells. Journal of Immunological Methods 2010 355 1-2 76 79 2-s2.0-77951206695 10.1016/j.jim.2010.02.006
    • (2010) Journal of Immunological Methods , vol.355 , Issue.1-2 , pp. 76-79
    • Greer, S.1    Honeywell, R.2    Geletu, M.3    Arulanandam, R.4    Raptis, L.5
  • 15
    • 13844313879 scopus 로고    scopus 로고
    • Housekeeping proteins: A preliminary study illustrating some limitations as useful references in protein expression studies
    • DOI 10.1002/pmic.200400941
    • Ferguson R. E., Carroll H. P., Harris A., Maher E. R., Selby P. J., Banks R. E., Banks R., Housekeeping proteins: a preliminary study illustrating some limitations as useful references in protein expression studies. Proteomics 2005 5 2 566 571 2-s2.0-13844313879 10.1002/pmic.200400941 (Pubitemid 40262073)
    • (2005) Proteomics , vol.5 , Issue.2 , pp. 566-571
    • Ferguson, R.E.1    Carroll, H.P.2    Harris, A.3    Maher, E.R.4    Selby, P.J.5    Banks, R.E.6    Banks, R.7
  • 16
    • 84884964957 scopus 로고    scopus 로고
    • Stain-free total protein staining is a superior loading control to β -actin for Western blots
    • Gilda J. E., Gomes A. V., Stain-free total protein staining is a superior loading control to β -actin for Western blots. Analytical Biochemistry 2013 440 2 186 188
    • (2013) Analytical Biochemistry , vol.440 , Issue.2 , pp. 186-188
    • Gilda, J.E.1    Gomes, A.V.2
  • 17
    • 38749142906 scopus 로고    scopus 로고
    • Protein production and purification
    • Genomics Consortium S. Structural Genomics Consortium C. Structural Genomics Consortium N
    • Structural Genomics Consortium, China Structural Genomics Consortium, Northeast Structural Genomics Consortium, Protein production and purification. Nature Methods 2008 5 2 135 146
    • (2008) Nature Methods , vol.5 , Issue.2 , pp. 135-146
  • 19
    • 77956291646 scopus 로고    scopus 로고
    • Homogenization of mammalian tissue
    • 10.1101/pdb.prot5455
    • Simpson R. J., Homogenization of mammalian tissue. Cold Spring Harbor Protocols 2010 10.1101/pdb.prot5455
    • (2010) Cold Spring Harbor Protocols
    • Simpson, R.J.1
  • 21
    • 84868029269 scopus 로고    scopus 로고
    • A MIQE Case Study - Effect of RNA Sample Quality and Reference Gene Stability on Gene Expression Data
    • Taylor S., Buchanan M., Basik M., A MIQE Case Study-Effect of RNA Sample Quality and Reference Gene Stability on Gene Expression Data. Bio-Rad Bulletin: 6245, 2011
    • (2011) Bio-Rad Bulletin , vol.6245
    • Taylor, S.1    Buchanan, M.2    Basik, M.3
  • 22
    • 0026521693 scopus 로고
    • High yield electroblotting onto polyvinylidene difluoride membranes from polyacrylamide gels
    • 2-s2.0-0026521693
    • Mozdzanowski J., Hembach P., Speicher D. W., High yield electroblotting onto polyvinylidene difluoride membranes from polyacrylamide gels. Electrophoresis 1992 13 1-2 59 64 2-s2.0-0026521693
    • (1992) Electrophoresis , vol.13 , Issue.1-2 , pp. 59-64
    • Mozdzanowski, J.1    Hembach, P.2    Speicher, D.W.3
  • 23
    • 0031149725 scopus 로고    scopus 로고
    • High-efficiency blotting of proteins of diverse sizes following sodium dodecyl sulfate-polyacrylamide gel electrophoresis
    • DOI 10.1006/abio.1997.2061
    • Bolt M. W., Mahoney P. A., High-efficiency blotting of proteins of diverse sizes following sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Analytical Biochemistry 1997 247 2 185 192 2-s2.0-0031149725 10.1006/abio.1997.2061 (Pubitemid 27200149)
    • (1997) Analytical Biochemistry , vol.247 , Issue.2 , pp. 185-192
    • Bolt, M.W.1    Mahoney, P.A.2
  • 25
    • 84870722152 scopus 로고    scopus 로고
    • Stain-free technology as normalization tool in western blot analysis
    • Guertler A., Kunz N., Gomolka M., Stain-free technology as normalization tool in western blot analysis. Analytical Biochemistry 2013 433 2 105 111
    • (2013) Analytical Biochemistry , vol.433 , Issue.2 , pp. 105-111
    • Guertler, A.1    Kunz, N.2    Gomolka, M.3
  • 26
    • 84875720543 scopus 로고    scopus 로고
    • An old method facing a new challenge: Re-visiting housekeeping proteins as internal reference control for neuroscience research
    • Li R., Shen Y., An old method facing a new challenge: re-visiting housekeeping proteins as internal reference control for neuroscience research. Life Sciences 2013 92 13 747 751
    • (2013) Life Sciences , vol.92 , Issue.13 , pp. 747-751
    • Li, R.1    Shen, Y.2
  • 27
    • 69449092635 scopus 로고    scopus 로고
    • Quantifying Western blots: Pitfalls of densitometry
    • 2-s2.0-69449092635 10.1002/elps.200800720
    • Gassmann M., Grenacher B., Rohde B., Vogel J., Quantifying Western blots: pitfalls of densitometry. Electrophoresis 2009 30 11 1845 1855 2-s2.0-69449092635 10.1002/elps.200800720
    • (2009) Electrophoresis , vol.30 , Issue.11 , pp. 1845-1855
    • Gassmann, M.1    Grenacher, B.2    Rohde, B.3    Vogel, J.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.