A single point mutation enhances hydroxynitrile synthesis by halohydrin dehalogenase

Enzyme and Microbial Technology

Volumn 70, Issue , 2015, Pages 50-57

  Schallmey, Marcus ^a   Jekel, Peter ^a   Tang, Lixia ^a,b   Majerić Elenkov, Maja ^a,c   Höffken, Hans Wolfgang ^d   Hauer, Bernhard ^d,e   Janssen, Dick B ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b UNIVERSITY OF ELECTRONIC SCIENCE AND TECHNOLOGY OF CHINA   (China)

^c RUDER BO KOVI INSTITUTE   (Croatia)

^d BASF SE   (Germany)

^e UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Cyanide; Epoxides; Halohydrin dehalogenase; Hydroxynitrile; Ligand binding; Protein engineering

Indexed keywords

AMINO ACIDS; BINDING SITES; CYANIDES; ENZYMES; HYDROGEN BONDS; NEGATIVE IONS; OXYGEN; SUBSTRATES;

EPOXIDES; HALOHYDRIN DEHALOGENASE; HYDROXYNITRILE; LIGAND BINDING; PROTEIN ENGINEERING;

ENZYME ACTIVITY;

BACTERIAL ENZYME; HALOHYDRIN DEHALOGENASE; HYDROXYNITRILE; NITRILE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; HYDROLASE; MO 113; OXADIAZOLE DERIVATIVE;

ARTICLE; BINDING SITE; ENANTIOSELECTIVITY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME SUBSTRATE; HYDROGEN BOND; NONHUMAN; POINT MUTATION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; STRUCTURE ANALYSIS; SYNTHESIS; AGROBACTERIUM TUMEFACIENS; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BIOTECHNOLOGY; CHEMISTRY; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; MOLECULAR GENETICS; PROTEIN ENGINEERING; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; STEREOISOMERISM; X RAY CRYSTALLOGRAPHY;

AGROBACTERIUM TUMEFACIENS; CORYNEBACTERIUM; MYCOBACTERIUM;

AGROBACTERIUM TUMEFACIENS; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; BIOTECHNOLOGY; CATALYTIC DOMAIN; CHEMISTRY TECHNIQUES, SYNTHETIC; CRYSTALLOGRAPHY, X-RAY; HYDROGEN BONDING; HYDROLASES; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NITRILES; OXADIAZOLES; POINT MUTATION; PROTEIN ENGINEERING; SEQUENCE HOMOLOGY, AMINO ACID; STEREOISOMERISM; SUBSTRATE SPECIFICITY;

EID: 84922260380     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2014.12.009     Document Type: Article

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