Enhancing the recombinant protein expression of halohydrin dehalogenase HheA in Escherichia coli by applying a codon optimization strategy

Enzyme and Microbial Technology

Volumn 49, Issue 4, 2011, Pages 395-401

  Tang, Lixia ^a   Jiang, Rongxiang ^a   Zheng, Kai ^a   Zhu, Xuechen ^a  

^a UNIVERSITY OF ELECTRONIC SCIENCE AND TECHNOLOGY OF CHINA   (China)

Author keywords

+2 Codon optimization; Halohydrin dehalogenase; PGEF(+) expression vector; Purification; Recombinant expression

Indexed keywords

AGROBACTERIUM RADIOBACTER AD1; ARTHROBACTER SP; CHIRAL BUILDING BLOCKS; CODON OPTIMIZATION; CODON USAGE; DEHALOGENASE; E. COLI; END REGIONS; EXPRESSION LEVELS; EXPRESSION VECTORS; GENERAL TOOLS; HALOHYDRINS; RECOMBINANT EXPRESSION; RECOMBINANT PROTEIN EXPRESSION; RECOMBINANT PROTEIN PRODUCTIONS; SECONDARY STRUCTURES; WILD TYPES;

ESCHERICHIA COLI; GENE ENCODING; OPTIMIZATION; PROTEINS; PURIFICATION;

GENE EXPRESSION;

BACTERIAL ENZYME; HALOHYDRIN DEHALOGENASE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTHROBACTER; ARTICLE; BIOCATALYST; CODON; CODON USAGE; ESCHERICHIA COLI; NONHUMAN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE;

ARTHROBACTER; BASE SEQUENCE; BIOTECHNOLOGY; CODON; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; HYDROLASES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; NUCLEIC ACID CONFORMATION; RECOMBINANT PROTEINS;

AGROBACTERIUM TUMEFACIENS; ARTHROBACTER SP.; ESCHERICHIA COLI;

EID: 80051784973     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2011.06.021     Document Type: Article

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