메뉴 건너뛰기




Volumn 5, Issue 2, 2007, Pages 318-323

Synthesis of enantiopure chloroalcohols by enzymatic kinetic resolution

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME KINETICS; REACTION KINETICS; SUBSTRATES; SYNTHESIS (CHEMICAL);

EID: 33846107813     PISSN: 14770520     EISSN: None     Source Type: Journal    
DOI: 10.1039/b613937j     Document Type: Article
Times cited : (44)

References (36)
  • 19
    • 0006170068 scopus 로고    scopus 로고
    • Per enzyme subunit, i.e. per active site. NB The active form of HheC is a tetramer consisting of four identical subunits (28 kD per monomer); each of the four monomers is catalytically active
    • M. Lautens M. L. Maddess E. L. O. Sauer S. G. Ouellet Org. Lett. 2002 4 83
    • (2002) Org. Lett. , vol.4 , pp. 83
    • Lautens, M.1    Maddess, M.L.2    Sauer, E.L.O.3    Ouellet, S.G.4
  • 23
    • 20644469267 scopus 로고
    • C10H11ClO, Mr = 182.65, orthorhombic, P212121, a = 4.9735(7), b = 10.531(2), c = 17.521(3) Å, V = 917.7(3) Å3, Z = 4, Dx = 1.322 g cm-3, F(000) = 384, = 3.63 cm-1, λ(MoK α) = 0.71073 Å, T = 100(1) K, 7911 reflections measured, GooF = 1.078, wR(F2) = 0.0698 for 2109 unique reflections and 153 parameters and R(F) = 0.0311 for 1994 reflections obeying Fo 4.0 σ(Fo) criterion of observability. The asymmetric unit consists of one molecule of the title compound; the molecules are linked into an infinite one-dimensional chain by O-H⋯O intermolecular bonds. The chiral center of C9 showed the S-configuration
    • C. S. Chen Y. Fujimoto G. Girdaukas C. Sih J. Am. Chem. Soc. 1982 104 7294
    • (1982) J. Am. Chem. Soc. , vol.104 , pp. 7294
    • Chen, C.S.1    Fujimoto, Y.2    Girdaukas, G.3    Sih, C.4
  • 28
    • 33846114901 scopus 로고
    • Engl. Ed. 1957 27 1309
    • Ponomarew et al. Zh. Obshch. Khim. 1957 27 1226 Engl. Ed. 1957 27 1309
    • (1957) Zh. Obshch. Khim. , vol.27 , pp. 1226
    • Ponomarew1
  • 34
    • 84981840463 scopus 로고
    • The buffer solution consisted of 0.15 M (NH4) 2SO4 in TEMG (10 mM Tris-sulfate, pH 7.5, 3 mM EDTA, 0.1% 2-mercaptoethanol, 10% glycerol) The solution contained 3.0-6.0 mg ml -1 of active enzyme. Thus, the amount of HheC used was 60-120 mg for analytical scale resolutions and 150-300 mg for preparative scale resolutions. The actual amount of active HheC in each individual resolution was checked prior to the reaction by a spectrophotometric assay, according to a method described in:
    • H. Hopff R. Wandeler Helv. Chim. Acta 1962 45 982
    • (1962) Helv. Chim. Acta , vol.45 , pp. 982
    • Hopff, H.1    Wandeler, R.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.