Smad2 protects against TGF-β1/Smad3-mediated collagen synthesis in human hepatic stellate cells during hepatic fibrosis

Molecular and Cellular Biochemistry

Volumn 400, Issue 1-2, 2015, Pages 17-28

  Zhang, Lei ^a   Liu, Changwei ^a   Meng, Xiao ming ^a   Huang, Cheng ^a   Xu, Fengyun ^a   Li, Jun ^a  

^a ANHUI MEDICAL UNIVERSITY   (China)

Author keywords

Hepatic fibrosis; Smad2; Smad3; TGF 1

Indexed keywords

COLLAGEN TYPE 1; GELATINASE A; MATRIX PROTEIN; SMAD2 PROTEIN; SMAD3 PROTEIN; SMALL INTERFERING RNA; TISSUE INHIBITOR OF METALLOPROTEINASE 1; TRANSFORMING GROWTH FACTOR BETA1; COLLAGEN; SMAD2 PROTEIN, HUMAN; SMAD3 PROTEIN, HUMAN;

ADULT; ANIMAL CELL; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CARBON TETRACHLORIDE-INDUCED LIVER FIBROSIS; COLLAGEN SYNTHESIS; CONTROLLED STUDY; ENZYME ACTIVITY; EXTRACELLULAR MATRIX; FIBROGENESIS; HISTOPATHOLOGY; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; MALE; NONHUMAN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; PROTEIN TARGETING; PROTEIN TRANSPORT; RAT; RNA INTERFERENCE; STELLATE CELL; ANIMAL; BIOSYNTHESIS; GENE EXPRESSION REGULATION; GENETICS; LIVER; LIVER CIRRHOSIS; METABOLISM; PATHOLOGY; SIGNAL TRANSDUCTION;

ANIMALS; COLLAGEN; GENE EXPRESSION REGULATION; HEPATIC STELLATE CELLS; HUMANS; LIVER; LIVER CIRRHOSIS; RATS; SIGNAL TRANSDUCTION; SMAD2 PROTEIN; SMAD3 PROTEIN; TISSUE INHIBITOR OF METALLOPROTEINASE-1; TRANSFORMING GROWTH FACTOR BETA1;

EID: 84922005506     PISSN: 03008177     EISSN: 15734919     Source Type: Journal    
DOI: 10.1007/s11010-014-2258-1     Document Type: Article

References (37)

1. Finnson KW, McLean S, Di Guglielmo GM, Philip A (2013) Dynamics of transforming growth factor beta signaling in wound healing and scarring. Adv Wound Care (New Rochelle) 2:195–214
2. Pakyari M, Farrokhi A, Maharlooei MK, Ghahary A (2013) Critical role of transforming growth factor beta in different phases of wound healing. Adv Wound Care (New Rochelle) 2:215–224
3. Wells RG (2000) Fibrogenesis. V. TGF-beta signaling pathways. Am J Physiol Gastrointest Liver Physiol 279:G845–G850
4. Mu Y, Gudey SK, Landstrom M (2012) Non-Smad signaling pathways. Cell Tissue Res 347:11–20
5. Chen J, Xia Y, Lin X, Feng XH, Wang Y (2014) Smad3 signaling activates bone marrow-derived fibroblasts in renal fibrosis. Lab Invest 94:545–556
6. Matsuzaki K (2013) Smad phospho-isoforms direct context-dependent TGF-beta signaling. Cytokine Growth Factor Rev 24:385–399
7. Kretschmer A, Moepert K, Dames S, Sternberger M, Kaufmann J, Klippel A (2003) Differential regulation of TGF-beta signaling through Smad2, Smad3 and Smad4. Oncogene 22:6748–6763
8. Yagi K, Goto D, Hamamoto T, Takenoshita S, Kato M, Miyazono K (1999) Alternatively spliced variant of Smad2 lacking exon 3. Comparison with wild-type Smad2 and Smad3. J Biol Chem 274:703–709
9. Nomura M, Li E (1998) Smad2 role in mesoderm formation, left-right patterning and craniofacial development. Nature 393:786–790
10. Yang X, Letterio JJ, Lechleider RJ, Chen L, Hayman R, Gu H, Roberts AB, Deng C (1999) Targeted disruption of SMAD3 results in impaired mucosal immunity and diminished T cell responsiveness to TGF-beta. EMBO J 18:1280–1291
11. Derynck R, Zhang YE (2003) Smad-dependent and Smad-independent pathways in TGF-beta family signalling. Nature 425:577–584
12. Heyer J, Escalante-Alcalde D, Lia M, Boettinger E, Edelmann W, Stewart CL, Kucherlapati R (1999) Postgastrulation Smad2-deficient embryos show defects in embryo turning and anterior morphogenesis. Proc Natl Acad Sci USA 96:12595–12600
13. Weinstein M, Yang X, Li C, Xu X, Gotay J, Deng CX (1998) Failure of egg cylinder elongation and mesoderm induction in mouse embryos lacking the tumor suppressor smad2. Proc Natl Acad Sci U S A 95:9378–9383
14. Datto MB, Frederick JP, Pan L, Borton AJ, Zhuang Y, Wang XF (1999) Targeted disruption of Smad3 reveals an essential role in transforming growth factor beta-mediated signal transduction. Mol Cell Biol 19:2495–2504
15. Zavadil J, Cermak L, Soto-Nieves N, Bottinger EP (2004) Integration of TGF-beta/Smad and Jagged1/Notch signalling in epithelial-to-mesenchymal transition. EMBO J 23:1155–1165
16. Roberts AB, Tian F, Byfield SD, Stuelten C, Ooshima A, Saika S, Flanders KC (2006) Smad3 is key to TGF-beta-mediated epithelial-to-mesenchymal transition, fibrosis, tumor suppression and metastasis. Cytokine Growth Factor Rev 17:19–27
17. Vindevoghel L, Lechleider RJ, Kon A, De Caestecker MP, Uitto J, Roberts AB, Mauviel A (1998) SMAD3/4-dependent transcriptional activation of the human type VII collagen gene (COL7A1) promoter by transforming growth factor beta. Proc Natl Acad Sci U S A 95:14769–14774
18. Chen SJ, Yuan W, Mori Y, Levenson A, Trojanowska M, Varga J (1999) Stimulation of type I collagen transcription in human skin fibroblasts by TGF-beta: involvement of Smad 3. J Invest Dermatol 112:49–57
19. Ueberham E, Low R, Ueberham U, Schonig K, Bujard H, Gebhardt R (2003) Conditional tetracycline-regulated expression of TGF-beta1 in liver of transgenic mice leads to reversible intermediary fibrosis. Hepatology 37:1067–1078
20. Su E, Han X, Jiang G (2010) The transforming growth factor beta 1/SMAD signaling pathway involved in human chronic myeloid leukemia. Tumori 96:659–666
21. ten Dijke P, Hill CS (2004) New insights into TGF-beta-Smad signalling. Trends Biochem Sci 29:265–273
22. Uemura M, Swenson ES, Gaca MD, Giordano FJ, Reiss M, Wells RG (2005) Smad2 and Smad3 play different roles in rat hepatic stellate cell function and alpha-smooth muscle actin organization. Mol Biol Cell 16:4214–4224
23. Liu C, Gaca MD, Swenson ES, Vellucci VF, Reiss M, Wells RG (2003) Smads 2 and 3 are differentially activated by transforming growth factor-beta (TGF-beta) in quiescent and activated hepatic stellate cells. Constitutive nuclear localization of Smads in activated cells is TGF-beta-independent. J Biol Chem 278:11721–11728
24. Gressner OA, Lahme B, Siluschek M, Rehbein K, Weiskirchen R, Gressner AM (2009) Connective tissue growth factor is a Smad2 regulated amplifier of transforming growth factor beta actions in hepatocytes–but without modulating bone morphogenetic protein 7 signaling. Hepatology 49:2021–2030
25. Ju W, Ogawa A, Heyer J, Nierhof D, Yu L, Kucherlapati R, Shafritz DA, Bottinger EP (2006) Deletion of Smad2 in mouse liver reveals novel functions in hepatocyte growth and differentiation. Mol Cell Biol 26:654–667
26. Meng XM, Huang XR, Chung AC, Qin W, Shao X, Igarashi P, Ju W, Bottinger EP, Lan HY (2010) Smad2 protects against TGF-beta/Smad3-mediated renal fibrosis. J Am Soc Nephrol 21:1477–1487
27. Verrecchia F, Chu ML, Mauviel A (2001) Identification of novel TGF-beta/Smad gene targets in dermal fibroblasts using a combined cDNA microarray/promoter transactivation approach. J Biol Chem 276:17058–17062
28. Yuan W, Varga J (2001) Transforming growth factor-beta repression of matrix metalloproteinase-1 in dermal fibroblasts involves Smad3. J Biol Chem 276:38502–38510
29. Piek E, Ju WJ, Heyer J, Escalante-Alcalde D, Stewart CL, Weinstein M, Deng C, Kucherlapati R, Bottinger EP, Roberts AB (2001) Functional characterization of transforming growth factor beta signaling in Smad2- and Smad3-deficient fibroblasts. J Biol Chem 276:19945–19953
30. Roberts AB, Russo A, Felici A, Flanders KC (2003) Smad3: a key player in pathogenetic mechanisms dependent on TGF-beta. Ann N Y Acad Sci 995:1–10
31. Tarasewicz E, Jeruss JS (2012) Phospho-specific Smad3 signaling: impact on breast oncogenesis. Cell Cycle 11:2443–2451
32. Flanders KC (2004) Smad3 as a mediator of the fibrotic response. Int J Exp Pathol 85:47–64
33. Masszi A, Kapus A (2011) Smaddening complexity: the role of Smad3 in epithelial-myofibroblast transition. Cells Tissues Organs 193:41–52
34. Latella G, Vetuschi A, Sferra R, Catitti V, D’Angelo A, Zanninelli G, Flanders KC, Gaudio E (2009) Targeted disruption of Smad3 confers resistance to the development of dimethylnitrosamine-induced hepatic fibrosis in mice. Liver Int 29:997–1009
35. Hayes S, Chawla A, Corvera S (2002) TGF beta receptor internalization into EEA1-enriched early endosomes: role in signaling to Smad2. J Cell Biol 158:1239–1249
36. Hu Y, Chuang JZ, Xu K, McGraw TG, Sung CH (2002) SARA, a FYVE domain protein, affects Rab5-mediated endocytosis. J Cell Sci 115:4755–4763
37. Di Guglielmo GM, Le Roy C, Goodfellow AF, Wrana JL (2003) Distinct endocytic pathways regulate TGF-beta receptor signalling and turnover. Nat Cell Biol 5:410–421