Genome editing of BmFib-H gene provides an empty Bombyx mori silk gland for a highly efficient bioreactor

Scientific Reports

Volumn 4, Issue , 2014, Pages

  Ma, Sanyuan ^a   Shi, Run ^a   Wang, Xiaogang ^a   Liu, Yuanyuan ^a   Chang, Jiasong ^a   Gao, Jie ^a   Lu, Wei ^a   Zhang, Jianduo ^a   Zhao, Ping ^a   Xia, Qingyou ^a  

^a SOUTHWEST UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

HYBRID PROTEIN; SILK;

ANIMAL; BIOSYNTHESIS; BOMBYX; GENE; GENE EXPRESSION; GENE INACTIVATION; GENE TARGETING; GENETICS; GENOMICS; INSECT GENOME; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; REPORTER GENE; SEQUENCE ALIGNMENT; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; BASE SEQUENCE; BOMBYX; GENE EXPRESSION; GENE KNOCKOUT TECHNIQUES; GENE TARGETING; GENES, INSECT; GENES, REPORTER; GENOME, INSECT; GENOMICS; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SILK;

EID: 84920984583     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep06867     Document Type: Article

References (39)

1. Wang, Y., Zhao, S., Bai, L., Fan, J. & Liu, E. Expression Systems and Species Used for Transgenic Animal Bioreactors. Biomed. Res. Int. 2013, 580463 (2013).
2. Askarieh, G. et al. Self-assembly of spider silk proteins is controlled by a pHsensitive relay. Nature. 465, 236-238 (2010).
3. Xia, Q., Li, S. & Feng, Q. Advances in Silkworm Studies Accelerated by the Genome Sequencing of Bombyx mori. Annu. Rev. Entomol. 59, 513-536 (2014).
4. Tomita, M. et al. Transgenic silkworms produce recombinant human type III procollagen in cocoons. Nat. Biotechnol. 21, 52-56 (2003).
5. Adachi, T., Tomita, M., Shimizu, K., Ogawa, S. & Yoshizato, K. Generation of hybrid transgenic silkworms that express Bombyx mori prolyl-hydroxylase alpha-subunits and human collagens in posterior silk glands: Production of cocoons that contained collagens with hydroxylated proline residues. J. Biotechnol. 126, 205-219 (2006).
6. Adachi, T. et al. Production of a non-triple helical collagen alpha chain in transgenic silkworms and its evaluation as a gelatin substitute for cell culture. Biotechnol. Bioeng. 106, 860-70 (2010).
7. Royer, C. et al. Biosynthesis and cocoon-export of a recombinant globular protein in transgenic silkworms. Transgenic Res. 14, 463-472 (2005).
8. Hino, R., Tomita, M. & Yoshizato, K. The generation of germline transgenic silkworms for the production of biologically active recombinant fusion proteins of fibroin and human basic fibroblast growth factor. Biomaterials. 27, 5715-5724 (2006).
9. Ogawa, S., Tomita, M., Shimizu, K. & Yoshizato, K. Generation of a transgenic silkworm that secretes recombinant proteins in the sericin layer of cocoon: production of recombinant human serum albumin. J. Biotechnol. 128, 531-44 (2007).
10. Kurihara, H., Sezutsu, H., Tamura, T. & Yamada, K. Production of an active feline interferon in the cocoon of transgenic silkworms using the fibroin H-chain expression system. Biochem. Biophys. Res. Commun. 355, 976-980 (2007).
11. Tateno, M. et al. Production and characterization of the recombinant humanmuopioid receptor from transgenic silkworms. J. Biochem. 145, 37-42 (2009).
12. Iizuka, M. et al. Production of a recombinant mouse monoclonal antibody in transgenic silkworm cocoons. FEBS J. 276, 5806-5820 (2009).
13. Teulé, F. et al. Silkworms transformed with chimeric silkworm/spider silk genes spin composite silk fibers with improved mechanical properties. Proc. Natl. Acad. Sci. USA. 109, 923-928 (2012).
14. Zhu, Z. et al. Mechanical properties of regenerated Bombyx mori silk fibers and recombinant silk fibers produced by transgenic silkworms. J. Biomater. Sci. Polym. Ed. 21, 395-411 (2010).
15. Wen, H. et al. Transgenic silkworms (Bombyx mori) produce recombinant spider dragline silk in cocoons. Mol. Biol. Rep. 37, 1815-1821 (2010).
16. Zhao, A. et al. New and highly efficient expression systems for expressing selectively foreign protein in the silk glands of transgenic silkworm. Transgenic Res. 19, 29-44 (2010).
17. Tomita, M. et al. A germline transgenic silkworm that secretes recombinant proteins in the sericin layer of cocoon. Transgenic Res. 16, 449-465 (2007).
18. Wang, F. et al. An optimized sericin-1 expression system for mass-producing recombinant proteins in the middle silk glands of transgenic silkworms. Transgenic Res. 22, 925-938 (2013).
19. Iizuka, M., Tomita, M., Shimizu, K., Kikuchi, Y. & Yoshizato, K. Translational enhancement of recombinant protein synthesis in transgenic silkworms by a 50-untranslated region of polyhedrin gene of bombyx mori nucleopolyhedrovirus. J. Biosci. Bioeng. 105, 595-603 (2008).
20. Inoue, S. et al. A fibroin secretion-deficient silkworm mutant, Nd-sD, provides an efficient system for producing recombinant proteins. Insect Biochem. Mol. Biol. 35, 51-59 (2005).
21. Beumer, K. J. et al. Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases. Proc. Natl. Acad. Sci.USA. 105, 19821-19826 (2008).
22. Carroll, D. Genome engineering with zinc-finger nucleases. Genetics. 188, 773-782 (2008).
23. Zhang, F. et al. Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat. Biotechnol. 29, 149-153 (2011).
24. Jinek, M. et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. 337, 816-821 (2012).
25. Takasu, Y. et al. Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection. Insect Biochem. Mol. Biol. 40, 759-765 (2010).
26. Ma, S. et al. Highly efficient and specific genome editing in silkwormusing custom TALENs. PLoS One. 7, e45035 (2012).
27. Zhou, C. Z. et al. Fine organization of Bombyx mori fibroin heavy chain gene. Nucleic Acids Res. 28, 2413-2419 (2000).
28. Xia, Q. et al. A draft sequence for the genome of the domesticated silkworm (Bombyx mori). Science. 306, 1937-1940 (2004).
29. Jones, D. et al. High-level expression of recombinant IgG in the human cell line per.c6. Biotechnol. Prog. 19, 163-168 (2003).
30. Shimura, K. Biochemical aspects on fibroin. Tanpakushitsu Kakusan Koso 24, 1324-1335 (1979).
31. Tomita, M. Transgenic silkworms that weave recombinant proteins into silk cocoons. Biotechnol. Lett. 33, 645-654 (2011).
32. Assenberg, R., Wan, P. T., Geisse, S. & Mayr, L. M. Advances in recombinant protein expression for use in pharmaceutical research. Curr. Opin. Struct. Biol. 23, 393-402 (2013).
33. Inoue, S. et al. Silk fibroin of Bombyx mori is secreted, assembling a high molecular mass elementary unit consisting of H-chain, L-chain, and P25, with a 6:6:1 molar. J. Biol. Chem. 275, 40517-40528 (2000).
34. Ma, S. et al. CRISPR/Cas9 mediated multiplex genome editing and heritable mutagenesis of BmKu70 in Bombyx mori. Sci. Rep. 4, 4489 (2014).
35. Liu, Y. et al. Highly efficient multiplex targeted mutagenesis and genomic structure variation in Bombyx mori cells using CRISPR/Cas9. Insect. Biochem. Mol. Biol. 49C, 35-42 (2014).
36. Ma, S. et al. Multiplex genomic structure variation mediated by TALEN and ssODN. BMC Genomics. 15, 41 (2014).
37. Doyon, Y. et al. Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases. Nat. Biotechnol. 26, 702-708 (2008).
38. Tamura, T. et al. Germline transformation of the silkworm Bombyx mori L. using a piggyBac transposon-derived vector. Nat Biotechnol. 18, 81-84 (2000).
39. Zhao, A. et al. Efficient strategies for changing the diapause character of silkworm eggs and for the germline transformation of diapause silkworm strains. Insect Sci. 19, 172-182 (2012).