Production of a non-triple helical collagen α chain in transgenic silkworms and its evaluation as a gelatin substitute for cell culture

Biotechnology and Bioengineering

Volumn 106, Issue 6, 2010, Pages 860-870

  Adachi, Takahiro ^a,b   Wang, Xiaobiao ^a   Murata, Tomoko ^a   Obara, Masanobu ^c   Akutsu, Hidenori ^d   Machida, Masakazu ^d   Umezawa, Akirihiro ^d   Tomita, Masahiro ^a  

^a Neosilk Co Ltd   (Japan)

^b Hiroshima Prefectural Institute of Industrial Science and Technology   (Japan)

^c HIROSHIMA UNIVERSITY   (Japan)

^d NATIONAL CENTER FOR CHILD HEALTH AND DEVELOPMENT   (Japan)

Author keywords

Cell culture; Cocoon; Collagen; Gelatin; Transgenic silkworm

Indexed keywords

BACULOVIRUS; CHAIN CONCENTRATION; CIRCULAR DICHROISM; COCOON; CYNOMOLGUS MONKEYS; EMBRYONIC STEM CELLS; GELATIN; HIGH QUALITY; HUMAN SKIN FIBROBLAST; HYDROXYLASE ACTIVITY; ITS EVALUATION; PLURIPOTENCY; SECONDARY STRUCTURES; SIMPLE METHOD; SPREADING RATE; TRANS-GENES; TRANSGENICS; TRIPLE HELICAL STRUCTURES; TRIPLE-HELICAL COLLAGEN; TYPE I COLLAGEN;

ANIMAL CELL CULTURE; ANIMALS; COLLAGEN; DICHROISM; SILK; STEM CELLS; TISSUE;

CELL CULTURE;

BIOMATERIAL; COLLAGEN TYPE 1; COLLAGEN TYPE 1 ALPHA1; GELATIN; HYBRID PROTEIN; HYDROXYPROLINE; PROCOLLAGEN PROLINE 2 OXOGLUTARATE 4 DIOXYGENASE; RECOMBINANT PROTEIN; TRANSACTIVATOR PROTEIN; UNCLASSIFIED DRUG; CULTURE MEDIUM; VIRUS PROTEIN;

ALPHA CHAIN; ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; BACULOVIRUS; CELL ADHESION; CELL SPREADING; CIRCULAR DICHROISM; COLLAGEN SYNTHESIS; EMBRYO; EMBRYONIC STEM CELL; HOMOZYGOSITY; HUMAN; HUMAN CELL; MONKEY; NONHUMAN; PLURIPOTENT STEM CELL; PROTEIN SECONDARY STRUCTURE; QUALITY CONTROL; SCID MOUSE; SILKWORM; SKIN FIBROBLAST; TERATOMA; TRANSGENE; TRANSGENIC ORGANISM; ANIMAL; BACULOVIRIDAE; BOMBYX; CHEMISTRY; CULTURE MEDIUM; CULTURE TECHNIQUE; FIBROBLAST; GENETICS; ISOLATION AND PURIFICATION; MACACA FASCICULARIS; METABOLISM; PROCEDURES; STEM CELL; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; BACULOVIRIDAE; BOMBYX; CELL CULTURE TECHNIQUES; CIRCULAR DICHROISM; COLLAGEN TYPE I; CULTURE MEDIA; FIBROBLASTS; HUMANS; MACACA FASCICULARIS; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; STEM CELLS; TRANS-ACTIVATORS; VIRAL PROTEINS;

BOMBYX MORI; MACACA FASCICULARIS; MUS;

EID: 77955700162     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22752     Document Type: Article

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