CRISPR/Cas9 mediated multiplex genome editing and heritable mutagenesis of BmKu70 in Bombyx mori

Scientific Reports

Volumn 4, Issue , 2014, Pages

  Ma, Sanyuan ^a   Chang, Jiasong ^a   Wang, Xiaogang ^a   Liu, Yuanyuan ^a   Zhang, Jianduo ^a   Lu, Wei ^a   Gao, Jie ^a   Shi, Run ^a   Zhao, Ping ^a   Xia, Qingyou ^a  

^a SOUTHWEST UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL; BOMBYX; CELL LINE; CRISPR CAS SYSTEM; GENE; GENE TARGETING; GENETICS; GENOTYPE; INSECT GENOME; MOLECULAR GENETICS; MUTAGENESIS; MUTATION; NUCLEOTIDE SEQUENCE; SEQUENCE ALIGNMENT; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; BASE SEQUENCE; BOMBYX; CELL LINE; CRISPR-CAS SYSTEMS; GENE TARGETING; GENES, INSECT; GENOME, INSECT; GENOTYPE; MOLECULAR SEQUENCE DATA; MUTAGENESIS; MUTATION; SEQUENCE ALIGNMENT;

EID: 84897068699     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep04489     Document Type: Article

References (35)

1. Pennisi, E. The crispr craze. Science 341, 833-836 (2013).
2. Mali, P. et al. RNA-guided human genome engineering via Cas9. Science 339, 823-826 (2013).
3. Cong, L. et al.Multiplex genome engineering using CRISPR/Cas systems. Science 339, 819-823 (2013).
4. Jinek, M. et al. RNA-programmed genome editing in human cells. Elife 2, e00471(2013).
5. Wang, H. et al. One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell 153, 910-918 (2013).
6. Yang, H. et al. One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering. Cell 154, 1370-1379 (2013).
7. Hu, X. et al. Heritable gene-targeting with gRNA/Cas9 in rats. Cell research 23, 1322-1325 (2013).
8. Hwang, W. Y. et al. Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol. 31, 227-229 (2013).
9. Jiang, W., Bikard, D., Cox, D., Zhang, F.&Marraffini, L. A. RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol. 31, 233-239 (2013).
10. Bassett, A. R., Tibbit, C., Ponting, C. P. & Liu, J. L. Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system. Cell report 4, 220-228 (2013).
11. DiCarlo, J. E. et al. Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Nucleic Acids Res. 41, 4336-4343 (2013).
12. Friedland, A. E. et al. Heritable genome editing in C. elegans via a CRISPR-Cas9 system. Nat Methods. 10, 741-743 (2013).
13. Shan, Q. et al. Targeted genome modification of crop plants using a CRISPR-Cas system. Nat Biotechnol. 31, 686-688 (2013).
14. Goldsmith, M. R., Shimada, T. & Abe, H. The genetics and genomics of the silkworm, Bombyx mori. Annu Rev Entomol 50, 71-100 (2005).
15. Teulé, F. et al. Silkworms transformed with chimeric silkworm/spider silk genes spin composite silk fibers with improved mechanical properties. Proc Natl Acad Sci USA. 109, 923-928 (2012).
16. Tamura, T. et al. Germline transformation of the silkworm Bombyx mori L. using a piggyBac transposon-derived vector. Nat Biotechnol. 18, 81-84 (2000).
17. Isobe, R. et al. Use of RNAi technology to confer enhanced resistance to BmNPV on transgenic silkworms. Arch Virol. 149, 1931-1940 (2004).
18. Takasu, Y. et al. Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection. Insect Biochem Mol Biol. 40, 759-765 (2010).
19. Ma, S. et al.Highly efficient and specific genome editing in silkwormusing custom TALENs. PLoS One 7, e45035 (2012).
20. Daimon, T., Kiuchi, T. & Takasu, Y. Recent progress in genome engineering techniques in the silkworm, Bombyx mori. Dev Growth Differ. doi:10.1111/dgd.12096 (2013).
21. Bertolini, L. R. et al. Increased Gene Targeting in Ku70 and Xrcc4 Transiently Deficient Human Somatic Cells. Mol Biotechnol. 41, 106-114.
22. Fattah, F. J., Lichter, N. F., Fattah, K. R., Oh, S. & Hendrickson, E. A. Ku70, an essential gene, modulates the frequency of rAAV-mediated gene targeting in human somatic cells. Proc Natl Acad Sci USA. 105, 8703-8708 (2008).
23. Näätsaari, L. et al. Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform Strain Generation for Gene Expression and Synthetic Biology. PLoS One. 7, e39720 (2012).
24. Qi, Y. et al. Increasing frequencies of site-specific mutagenesis and gene targeting in Arabidopsis by manipulating DNA repair pathways. Genome res. 23, 547-554 (2013).
25. Sawada, M. et al. Ku70 suppresses the apoptotic translocation of Bax to mitochondria. Nat Cell Biol 5, 320-329 (2003).
26. Koike, M. Dimerization, translocation and localization of Ku70 and Ku80 proteins. J Rad Res 43, 223-236 (2002).
27. Monferran, S., Muller, C., Mourey, L., Frit, P. & Salles, B. The membraneassociated form of the DNA repair protein Ku is involved in cell adhesion to fibronectin. J Mol Biol 337, 503-511 (2004).
28. Katz, D. J., Beer, M. A., Levorse, J. M. & Tilghman, S. M. Functional characterization of a novel Ku70/80 pause site at the H19/Igf2 imprinting control region. Mol Cell Biol 25, 3855-3863 (2005).
29. Boulton, S. J. & Jackson, S. P. Components of the Ku-dependent non-homologous end-joining pathway are involved in telomeric length maintenance and telomeric silencing. EMBO J. 17, 1819-1828 (1998).
30. Wang, F. et al. An optimized sericin-1 expression system for mass-producing recombinant proteins in the middle silk glands of transgenic silkworms. Transgenic Res. 22, 925-938 (2013).
31. Tanaka, H. et al. Construction of shRNA expression plasmids for silkworm cell lines using single-stranded DNA and Bst DNA polymerase. Methods Mol Biol. 942, 347-355 (2013).
32. Ma, S. et al. Multiplex genomic structure variation mediated by TALEN and ssODN. BMC Genomics. 15, 41 (2014).
33. Mon, H., Kusakabe, T., Lee, J. M., Kawaguchi, Y. & Koga, K. In vivo DNA doublestrand breaks enhance gene targeting in cultured silkworm cells. Comp Biochem Physiol B Biochem Mol Biol. 139, 99-106 (2004).
34. Tomita, M. et al. Transgenic silkworms produce recombinant human type III procollagen in cocoons. Nat Biotechnol. 21, 52-56 (2003).
35. Wang, Y. et al. The CRISPR/Cas System mediates efficient genome engineering in Bombyx mori. Cell Res. doi:10.1038/cr.2013.146 (2013).