A multi-faceted approach to understanding male infertility: gene mutations, molecular defects and assisted reproductive techniques (ART)

Journal of Assisted Reproduction and Genetics

Volumn 31, Issue 9, 2014, Pages 1115-1137

  Tahmasbpour, Eisa ^a   Balasubramanian, Dheepa ^b   Agarwal, Ashok ^c  

^a ISLAMIC AZAD UNIVERSITY   (Iran)

^b CASE WESTERN RESERVE UNIVERSITY   (United States)

^c LERNER RESEARCH INSTITUTE   (United States)

Author keywords

AZF region; Epigenetics; Male infertility; Molecular genetics; Spermatogenesis; Y chromosome

Indexed keywords

5,10 METHYLENETETRAHYDROFOLATE REDUCTASE (FADH2); ANDROGEN RECEPTOR; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; ESTROGEN RECEPTOR; FOLLITROPIN RECEPTOR; LUTEINIZING HORMONE RECEPTOR; MICRORNA; MITOCHONDRIAL DNA; RNA; SEX HORMONE BINDING GLOBULIN; SMALL INTERFERING RNA;

ANEUPLOIDY; AUTOSOME; AZFA GENE; AZFB GENE; AZFC GENE; CHROMOSOME ABERRATION; CHROMOSOME TRANSLOCATION; DNA MODIFICATION; EPIGENETICS; GENE; GENE INTERACTION; GENE MUTATION; HUMAN; INFERTILITY THERAPY; INSL3 GENE; KALLMANN 1 GENE; KLINEFELTER SYNDROME; LGR8 GENE; MALE; MALE INFERTILITY; PROTEIN INTERACTION; PROTEOMICS; REVIEW; SPERM; TAF7L GENE; TRANSCRIPTOMICS; USP26 GENE; Y CHROMOSOME; GENETICS; METABOLISM; MUTATION; PHYSIOLOGY; SEX CHROMOSOME ABERRATION; SPERMATOZOON;

ANEUPLOIDY; DNA, MITOCHONDRIAL; EPIGENOMICS; HUMANS; INFERTILITY, MALE; MALE; MICRORNAS; MUTATION; REPRODUCTIVE TECHNIQUES, ASSISTED; RNA, SMALL INTERFERING; SEX CHROMOSOME ABERRATIONS; SPERMATOZOA;

EID: 84920387555     PISSN: 10580468     EISSN: 15737330     Source Type: Journal    
DOI: 10.1007/s10815-014-0280-6     Document Type: Review

References (227)

1. Vogt PH. Molecular genetic of human male infertility: from genes to new therapeutic perspectives. Curr Pharm Des. 2004;10:1–29.
2. Ji G, Long Y, Zhou Y, Huang C, Gu A, Wang X. Common variants in mismatch repair genes associated with increased risk of sperm DNA damage and male infertility. BMC Med. 2012;10:1–10.
3. De Kretser DM. Male infertility. Lancet. 1997;349:787–90.
4. Dada R, Thilagavathi J, Venkatesh S, Esteves SC, Agarwal A. Genetic testing in male infertility. Open Reprod Sci J. 2011;3:42–56.
5. Varghese AC, du Plessis SS, Agarwal A. Male gamete survival at stake: causes and solutions. Reprod Bio Med Online. 2008;17:866–80.
6. Matzuk MM, Lamb DJ. Genetic dissection of mammalian fertility pathways. Nat Cell Biol. 2008;4:41–9.
7. Benoff S, Jacob A, Hurley IR. Male infertility and environmental exposure to lead and cadmium. Hum Reprod. 2000;6:107–21.
8. Sharpe RM. Lifestyle and environmental contribution to male infertility. Br Med Bull. 2000;56630–642.
9. Hosseinzadeh Colagar A, Jorsaraee GA, Tahmasbpour ME. Cigarette smoking and the risk of male infertility. Pakistan Biol Sci. 2007;10(21):3870–4.
10. Hosseinzadeh Colagar A, Tahmasbpour ME. Ascorbic acid levels in seminal plasma of fertile and idiopathic infertile men: determination and its relationship to sperm quality. J Clin Biochem Nutr. 2009;45:144–9.
11. Hosseinzadeh Colagar A, Tahmasbpour Marzony E, Chaichi MJ. Zinc levels in seminal plasma are associated with sperm quality in fertile and infertile men. Nutr Res. 2009;29:82–8.
12. Agarwal A, Allamaneni SSR. Oxidants and antioxidants in human fertility. Middle East Fertil Society J. 2004;9:187–97.
13. Hosseinzadeh Colagar A, Pouamir M, Tahmasbpour Marzony E, Jorsaraee GA. Relationship between seminal malondialdehyde and sperm parameters quality in the fertile and infertile men. Brazilian Archive Biol Tech. 2009;52(6):1387–92.
14. Krausz C, Forti G, Mcelreavey K. The Y chromosome and male fertility and infertility. Int J Androl. 2003;26:70–5.
15. Krausz C, Degl’Innocenti S, Nuti F, Morelli A, Felici F, Sansone M, et al. Natural transmission of USP9Y gene mutations: a new perspective on the role of AZFa genes in male fertility. Hum Mol Genet. 2006;15:2673–81.
16. Walsh TJ, Pera RR, Turek PJ. The genetics of male infertility. Semin Reprod Med. 2009;27:124–36.
17. O'Flynn O’Brien KL, Varghese AC, Agarwal A. The genetic causes of male factor infertility: A review. Fertil Steril. 2010;93:1–12.
18. Asero P, La Vignera S, Lanzafame F. Genetic aspects of male infertility. J Androl Sci. 2010;17:1–16.
19. Tüttelmann F, Simoni M. Current Recommendations for Genetic Testing in Male Infertility. European Urological Review, 2008; 88-92.
20. Ferlin A, Raicu F, Gatta V, Zuccarello D, Palka G, Foresta C. Male infertility: role of genetic background. Reprod Biomed Online. 2007;14:734–45.
21. Maduro MR, Lamb DJ. Understanding the new genetics of male infertility. J Urol. 2002;168:2197–205.
22. Ferlin A, Arredi B, Foresta C. Genetic causes of male infertility. Reprod Toxicol. 2006;22:133–41.
23. Schiff JD, Palermo GD, Veeck LL, Goldstein M, Rosenwaks Z, Schlegel PN. Success of testicular sperm extraction and intracytoplasmic sperm injection in men with Klinefelter syndrome. J Clin Endocrinol Metab. 2005;90(11):6263–7.
24. Visootsak J, Graham Jr JM. Klinefelter syndrome and other sex chromosomal aneuploidies. Orphanet J of Rare Diseases. 2006;1:42. Available from: http://www.OJRD.com/content/1/1/42.
25. Ichioka K, Utsunomiya N, Kohei N, Ueda N, Inoue K, Terai A. Adult onset of declining spermatogenesis in a man with nonmosaic Klinefelter's syndrome. Fertil Steril, 2006; 85(5): 1511 e1-2.
26. Therman E, Susman M. Human Chromosome: Structure, Behaviour and Effects. New York: Springer-Verlag; 1993.
27. De Braekeleer M, Dao TN. Cytogenetic studies in male infertility: a review. Hum Reprod. 1991;6:245–50.
28. Ananthapur V, Avvari S, Tella S, Nallari P, Akka J. A Robertsonian Translocation rob (14;15) (q10:q10) in a patient with recurrent abortions: A case report. J Reprod Infertil. 2010;11(3):197–200.
29. Georgiou I, Syrrou M, Pardalidis N, Karakitsios K, Mantzavinos T, Giotitsas ND, et al. Genetic and epigenetic risks of intracytoplasmic sperm injection method. Asian J Androl. 2006;8:643–73.
30. Keymolen K, Van Berkel K, Vorsselmans A, Staessen C, Liebaers L. Pregnancy Outcome in Carriers of Robertsonian Translocations. Am J Med Genet Part A. 2011;155:2381–5.
31. Gunel M, Cavkaytar S, Ceylaner G, Batioglu S. Azoospermia and cryptorchidism in a male with a de novo reciprocal t(Y;16) translocation. Genet Couns. 2008;19:277–80.
32. Scriven PN, Flinter FA, Braude PR, Ogivie CM. Robertsonian translocations—reproductive risks and indications for preimplantation gen-etic diagnosis. Hum Reprod. 2001;16:2267–73.
33. Papadimas J, Goulis DG, Giannouli C, Papanicolaou A, Tarlatzis B, Bontis JN. Ambiguous genitalia, 45, X/46, XY mosaic karyotype, and Y chromosome microdeletions in a 17-year-old man. Fertil Steril. 2001;76:1261–3.
34. Vogt PH. Human chromosome deletions in Yq11, AZF candidate genes and male infertility: history and update. Mol Hum Reprod. 1998;4:739–44.
35. Van der Auwera B, Van Roy N, De Paepe A, Hawkins JR, Liebaers I, Castedo S, et al. Molecular cytogenetic analysis of XX males using Y-specific DNA sequences, including SRY. Hum Genet. 1992;89:23–8.
36. Abusheikha N, Lass A, Brisden P. XX males without SRY gene and with infertility. Hum Reprod. 2001;16:717–8.
37. Vogt PH, Fernandes S. Polymorphic DAZ gene family in poly-morphic structure of AZFc locus: artwork or functional for human sperma-togenesis? Acta Pathol Microbiol Immunol Scand. 2003;111:115–27.
38. Foresta C, Ferlin A, Garolla A, Moro E, Pistorello M, Barbaux S, et al. High frequency of well-defined Y-chromosome deletions in idiopathic Sertoli cell-only syndrome. Hum Reprod. 1998;13:302–7.
39. van der Ven K, Montag M, Peschka B, Leygraaf J, Schwanitz G, Haidl G, et al. Combined cytogenetic and Y chromosome microdeletion screening in males undergoing intracytoplasmic sperm injection. Mol Hum Reprod. 1997;3:699–704.
40. Vogt PH, Edelmann A, Kirsch S, Henegariu O, Hirschmann P, Kiesewetter F, et al. Human Y chromo-some azoospermia factors (AZF) mapped to different subregions in Yq11. Hum Mol Genet. 1991;5:933–43.
41. Foresta C, Moro E, Ferlin A. Y chromosome microdeletions and alterations of spermatogenesis. Endocr Rev. 2001;22:226–39.
42. Muslumanoglu MH, Turgut M, Cilingir O, Can C, Ozyurek Y, Artan S. Role of the AZFd locus in spermatogenesis. Fertil Steril. 2005;84:519–22.
43. Sertic J, Cvitkovic P, Myers A, Saiki RK, Rukavina AS. Genetic Markers of Male Infertility: Y Chromosome Microdeletions and Cystic Fibrosis Transmembrane Conductance Gene Mutations. Croat Med J. 2001;42(4):416–20.
44. Silber SJ, Alagappan R, Brown LG, Page DC. Y chromosome deletions in azoospermic and severely oligozoospermic men undergoing intracytoplasmic sperm injection after testicular sperm extraction. Hum Re-prod. 1998;13:3332–7.
45. Fujisawa M, Shirakawa T, Kanzaki M, Okada H, Arakawa S, Kamidono S. Y-chromosome microdeletion and phenotype in cytogenetically normal men with idiopathic azoospermia. Fertil Steril. 2001;76:491–5.
46. Selva DM, Hammond GL. Human sex hormone-binding globulin is expressed in testicular germ cells and not in Sertoli cells. Horm Metab Res. 2006;38:230–5.
47. Selva DM, Hogeveen KN, Seguchi K, Tekpetey F, Hammond GL. A human sex hormone-binding globulin isoform accumulates in the acrosome during spermatogenesis. J Biol Chem. 2002;277:45291–8.
48. Foresta C, Ferlin A, Moro E. Deletion and expression analysis of AZFa genes on the human Y chromosome revealed a major role for DBY in male infertility. Hum Mol Genet. 2000;9:1161–9.
49. Vogt PH. Azoospermia factor (AZF) in Yq11: towards a molecular understanding of its function for human male fertility and spermatogenesis. Reprod Biomed Online. 2005;10:81–93.
50. Galan JJ, Guarducci E, Nuti F, Gonzalez A, Ruiz M, Ruiz A, et al. Molecular analysis of estrogen receptor alpha gene AGATA haplotype and SNP12 in European populations: potential protective effect for cryptorchidism and lack of association with male infertility. Hum Reprod. 2007;22:444–9.
51. Delbridge ML, Harry JL, Toder R, O'Neill RJ, Ma K, Chandley AC, et al. A human candidate spermatogenesis gene, RBM1, is conserved and amplified on the marsupial Y chromosome. Nat Genet. 1997;15:131–6.
52. Vogt PH. AZF deletions and Y chromosomal haplogroups: history and update based on sequence. Hum Reprod Update. 2005;11:319–36.
53. Elliot DJ, Millar MR, Oghene K, Ross A, Kiesewetter F, Pryor J, et al. Expression of RBM in the nuclei of human germ cells is dependent on a critical region of the Y chromosome long arm. Proc Natl Acad Sci. 1997;94:3848–53.
54. Lahn BT, Tang ZL, Zhou J, Barndt RJ, Parvinen M, Allis CD, et al. Previously uncharacterized histone acetyltransferases implicated in mammalian spermatogenesis. Proc Natl Acad Sci U S A. 2002;99:8707–12.
55. Mahanta R, Gogoi A, Chaudhury PNB, Roy S, Bhattacharyya IK, Sharma P, et al. Microdeletions in the Y Chromosome of Infertile Males with Azoospermia, Oligozoospermia and Asthenozoospermia from Assam, India. World Applied Sciences Journal. 2012;17(10):1265–70.
56. Vineeth VS, Malini SS. A Journey on Y Chromosomal Genes and Male Infertility. Int J Hum Genet. 2011;11(4):203–15.
57. de Carvalho CMB, Zuccherato LW, Fujisawa M, Shirakawa T, Ribeiro-dos-Santos AKC, Santos SEB, et al. Study of AZFc partial deletion gr/gr in fertile and infertile Japanese males. J Hum Genet. 2006;51:794–9.
58. Krausz C, Quintana-Murci L. McElreaveyK. Prognostic value of Y deletion analysis: what is the clinical prognostic value of Y chromosome microdeletion analysis? Hum Re-prod. 2000;15:1431–4.
59. Pinho MJ, Neves R, Costa P, Ferras C, Sousa M, Alves C, et al. Unique t(Y;1)(q12;q12) reciprocal translocation with loss of the heterochromatic region of chromosome 1 in a male with azoospermia due to meiotic arrest: a case report. Hum Reprod. 2005;20:689–96.
60. Zhang J, Qiu SD, Li SB, Zhou DX, Tian H, Huo YW, et al. Novel mutations in ubiquitin-specific protease 26 gene might cause spermatogenesis impairment and male infertility. Asian J Androl. 2007;9(6):809–14.
61. Skaletsky H, Kuroda-Kawaguchi T, Minx PJ, Cordum HS, Hillier L, Brown LG, et al. The male-specific region of the human Y chromosome is a mosaic of discrete sequence classes. Nature. 2003;423:825–37.
62. Repping S, Skaletsky H, Brown L, van Daalen SK, Korver CM, Pyntikova T, et al. Polymorphism for a 1.6-Mb deletion of the human Y chromosome persists through balance between recurrent mutation and haploid selection. Nat Genet. 2003;35:247–51.
63. Lardone MC, Parodi DA, Ebensperger M, Penaloza P, Cornejo V, Valdevenito R, et al. AZFc partial deletions in Chilean men with severe sper-matogenic failure. Fertil Steril. 2007;88:1318–26.
64. Hopps CV, Mielnik A, Goldstein M, Palermo GD, Rosenwaks Z, Schlegel PN. Detection of sperm in men with Y chromosome microdeletions of the AZFa, AZFb and AZFc regions. Hum Reprod. 2003;18:1660–5.
65. Eloualid A, Rhaissi H, Reguig A, Bounaceur S. El houate B, Abidi O, Charif M, Louanjli N, Chadli E, Barakat A, Bashamboo A, McElreavey K, Rouba H. Association of Spermatogenic Failure with the b2/b3 Partial AZFc Deletion. PLoS ONE. 2012;7(4):e34902.
66. Repping S, van Daalen SK, Korver CM, Brown LG, Marszalek JD, Gianotten J, et al. A family of human Y chromosomes has dispersed throughout northern eurasia despite a 1.8-Mb deletion in the azoospermia factor c region. Genomics. 2004;83:1046–52.
67. de Llanos M, Ballesca JL, Gazquez C, Margarit E, Oliva R. High frequency of gr/gr chromosome Y deletions in consecutive oligospermic ICSI candidates. Hum Reprod. 2005;20:216–20.
68. Tuttelmann F, Rajpert-De Meyts E, Nieschlag E, Simoni M. Gene polymorphisms and male infertility- a meta-analysis and literature review. RBM Online. 2007;15:643–58.
69. Stouffs K, Lissens W, Tournaye H, Haentjens P. What about gr/gr deletions and male infertility? Systematic review and meta-analysis. Hum Reprod Update. 2011;17:197–209.
70. Ferlin A, Moro E, Garolla A, Foresta C. Human male infertility and Y chromosome deletions: role of the AZF-candidate genes DAZ, RBM and DFFRY. Hum Reprod. 1999;14:1710–6.
71. Kuo PL, Wang ST, Lin YM, Lin YH, Teng YN, Hsu CC. Expression profiles of the DAZ gene family in human testis with and without spermatogenic failure. Fertil Steril. 2004;81:1034–40.
72. Teng YN, Lin YM, Lin YH, Tsao SY, Hsu CC, Lin SJ, et al. Association of a single-nucleotide polymorphism of the deleted-in-azoospermia-like gene with susceptibility to spermatogenic failure. J Clin Endocrinol Metab. 2002;87:5258–64.
73. Fernandes S, Huellen K. Gonc¸alves J, Dukal H, Zeisler J, De Meyts ER, Skakkebaek NE, Habermann B, Krause W, Sousa M et al. High frequency of DAZ1/DAZ2 gene deletions in patients with severe oligo-zoospermia. Mol Hum Reprod. 2002;8:286–98.
74. Ravel C, Chantot-Bastaraud S, Houate BE, Rouba H, Legendre M, Lorencxo D, et al. Y-chromosome AZFc structural architecture and relationship to male fertility. Fertil Steril. 2009;92:1924–33.
75. Sadeghi-Nejad H, Farrokhi F. Genetics of azoospermia: current knowledge, clinical implications, and future directions. Part II. Y chromosome microdeletions. Urol J. 2007;4:192–206.
76. Knower KC, Kelly S, Harley VR. Turning on the male-SRY, SOX9 and sex determination in mammals. Cytogenet Genome Res. 2003;101:185–98.
77. de Carvalho CMB, Santos FR. Human Y-chromosome variation and male dysfunction. J of Mol and Genet Med. 2005;1(2):63–75.
78. Shahid M, Dhillion VS, Jain N, Hedau S, Diwakar S, Sachdeva P, et al. Two new novel point mutations localized upstream and downstream of the HMG box region of the SRY gene in three Indian 46, XY females with sex reversal and gonadal tumour formation. Mol Hum Reprod. 2004;10:521–6.
79. Dasari VK, Goharderakhshan RZ, Perinchery G, Li LC, Tanaka Y, Alonzo J, et al. Expression analysis of Y chromosome genes in human prostate cancer. Urol. 2001;165:1335–41.
80. Eckardstein S, Cooper TG, Rutscha K, Meschede D, Horst J, Nieschlag E. Seminal plasma characteristics as indicators of cystic fibrosis transmembrane conductance regulator (CFTR) gene mutations in men with obstructive azoospermia. Fertil Steril. 2000;73:1226–31.
81. Chillon M, Casals T, Mercier B, Bassas L, Lissens W, Silber S, et al. Mutations in the cystic fibrosis gene in patients with congenital absence of the vas deferens. N Engl J Med. 1995;332(22):1475–80.
82. Hiort O, Holterhus PM, Horter T, Schulze W, Kremke B, Bals-Pratsch M, et al. Significance of mutations in the androgen receptor gene in males with idiopathic infertility. J Clin Endocrinol Metab. 2000;85(8):2810–5.
83. Yong EL, Tut TG, Ghadessy FJ, Prins G, Ratnam SS. Partial androgen insensitivity and correlations with the predicted three dimensional structure of the androgen receptor ligand-binding domain. Mol Cell Endocrinol. 1998;137:41–50.
84. Mifsud A, Sim CK, Boettger-Tong H, Moreira S, Lamb DJ, Lipshultz LI, et al. Trinucleotide (CAG) repeat polymorphisms in the androgen receptor gene: molecular markers of risk for male infertility. Fertil Steril. 2001;75:275–81.
85. Mengual L, Oriola J, Ascaso C, Ballesca JL, Oliva R. An Increased CAG Repeat Length in the Androgen Receptor Gene in Azoospermic ICSI Candidates. J Androl. 2003;24:279–84.
86. Cram DS, Song B, McLachlan RI, Trounson AO. CAG trinucleotide repeats in the androgen receptor gene of infertile men exhibit stable inheritance in female offspring conceived after ICSI. Mol Hum Reprod. 2000;6(9):861–6.
87. Foresta C, Ferlin A. Role of INSL3 and LGR8 in cryptorchidism and testicular functions. Reprod BioMed Online. 2004;9:294–8.
88. Mokosch A, Bernecker C, Willenberg HS, Neumann NJ. Kallmann syndrome. Hautarzt. 2011;62:728–30.
89. Hwang SH, Lee SM, Seo EJ, Choi KU, Park HJ, Park NC, et al. A case of male infertility with a reciprocal translocation t(X;14)(p11.4;p12). Korean J Lab Med. 2007;27(2):139–42.
90. Marques CJ, Costa P, Vaz B, Carvalho F, Fernandes S, Barros A, et al. Abnormal methylation of imprinted genes in human sperm is associated with oligozoospermia. Mol Hum Reprod. 2008;14:67–74.
91. Kelly TL, Neaga OR, Schwahn BC, Rozen R, Trazler JM. Infertility in 5, 10 methylenetetrahydrofolate reductase (MTHFR)-deficient male mice is partially alleviated by lifetime dietary betaine supplementation. Biol Reprod. 2005;72:667–77.
92. Singh AR, Vrtel R, Vodicka R, Dhaifalah I, Konvalinka D, Janikova M, et al. Y Chromosome and Male Infertility. Int J Hum Genet. 2005;5(4):225–35.
93. Singh K, Singh SK, Raman R. MTHFR A1298C polymorphism and idiopathic male infertility. J Grad Med. 2010;56:267–9.
94. Lazaros L, Xita N, Kaponis A, Zikopoulos K, Sofikitis N, Georgiou I. Evidence for association of sex hormone-binding globulin and androgen receptor genes with semen quality. Andrologia. 2008;40:186–91.
95. Rochira V, Granata AR, Madeo B, Zirilli L, Rossi G, Carani C. Estrogens in males: what have we learned in the last 10 years?Asian. J Androl. 2005;7:3–20.
96. Sinkevicius KW, Laine M, Lotan TL, Woloszyn K, Richburg JH, Greene GL. Estrogen-dependent and independent estrogen receptor-alpha signaling separately regulate male fertility. Endocrinology. 2009;150:2898–905.
97. Ferlin A, Tessari A, Ganz F, Marchina E, Barlati S, Garolla A, et al. Association of partial AZFc region deletions with spermatogenic impairment and male infertility. J Med Genet. 2005;42:209–13.
98. Suzuki Y, Sasagawa I, Itoh K. AshidaJ, Muroya K, Ogata T. Estrogen receptor alpha gene polymorphismis associated with idiopathic azoospermia. Fertil Steril. 2002;78:1341–3.
99. Robertson KM, O’Donnell L, Jones ME, Meachem SJ, Boon WC, Fisher CR, et al. Impairment of sperma-togenesis in mice lacking a functional aromatase (cyp 19) gene. Proc Natl Acad Sci. 1999;96:7986–91.
100. Guarducci E, Nuti F, Becherini L, Rotondi M, Balercia G, Forti G, et al. Estrogen receptor α promoter polymorphism: stronger estrogen action is coupled with lower sperm count. Hum Reprod. 2006;21:994–1001.
101. Krege JH, Hodgin JB, Couse JF, Enmark E, Warner M, Mahler JF, Sar M, Korach KS, Gustafsson JA, Smithies O. Generation and reproductive phenotypes of mice lacking estrogen receptor beta.Proc Natl Acad Sci, 1998; 95:15677.
102. Aschim EL, Giwercman A, Stahl O, Eberhard J, Cwikiel M, Nordenskjold A, et al. The RsaI polymorphism in the estrogen receptor-beta gene is associated with male infertility. J Clin Endocrinol Metab. 2005;90:5343.
103. Khattri A, Pandey RK, Gupta NJ, Chakravarty B, Deendayal M, Singh L, et al. CA repeat and RsaI polymorphisms in ERbeta gene are not associated with infertility in Indian men. Int J Androl. 2007;32:81–7.
104. Khattri A, Pandey RK, Gupta NJ, Chakravarty B, Deenadayal M, Singh L, et al. Estrogen receptor β gene mutations in Indian infertile men. Mol Hum Reprod. 2009;15:513–20.
105. Balkan M, Gedik A, Akkoc H, Ay O, Erdal ME, Isi H, et al. FSHR Single Nucleotide Polymorphism Frequencies in Proven Fathers and Infertile Men in Southeast Turkey. J Biomed Biotechnol. 2010;2010:1–5.
106. Zamudio NM, Chong S, O’Bryan MK. Epigenetic regulation in male germ cells. Reproduction. 2008;136:131–46.
107. Zhang F, Lu C, Li Z, Xie P, Xia Y, Zhu X, et al. Partial deletions are associated with an increased risk of complete deletion in AZFc: a new insight into the role of partial AZFc deletions in male infertility. J Med Genet. 2007;44:437–44.
108. Zhang J, Shao XG, Shi YB, Yan L, Wang L, Tian H, et al. Polymorphism of Usp26 correlates with idiopathic male infertility. Zhonghua Nan Ke Xue. 2012;18(2):105–8.
109. Lee IW, Kuan LC, Lin CH, Pan HA, Hsu CC, Tsai YC, et al. Association of USP26 haplotypes in men in Taiwan, China with severe spermatogenic defect. Asian J Androl. 2008;10:896–904.
110. Ribarski I, Lehavi O, Yogev L, Hauser R, Bar-Shira Maymon B, Botchan A, et al. USP26 gene variations in fertile and infertile men. Hum Reprod. 2009;24:477–84.
111. Stouffs K, Lissens W, Tournaye H, Van Steirteghem A, Liebaers I. Possible role of USP26 in patients with severely impaired spermatogenesis. Eur J Hum Genet. 2005;13:336–40.
112. Cheng Y, Buffone MG, Kouadio M, Goodheart M, Page DC, Gerton GL, Davidson I, Wang PJ. Abnormal Sperm in Mice Lacking the Taf7l Gene. Molecular and Cellular Biology, 2007; 2582-2589.
113. Falender AE, Freiman RN, Geles KG, Lo KC, Hwang K, Lamb DJ, et al. Maintenance of spermatogenesis requires TAF4b, a gonad-specific subunit of TFIID. Genes Dev. 2005;19:794–803.
114. Akinloye O, Gromoll J, Callies C, Nieschlag E, Simoni M. Mutation analysis of the X-chromosome linked, testis-specific TAF7L gene in spermatogenic failure. Andrologia. 2007;39:190–5.
115. Kumar DP, Sangeetha N. Mitochondrial DNA mutations and male infertility. Indian J Hum Genet. 2009;15(3):93–7.
116. Taylor RW, Turnbull DM. Mitochondrial DNA mutations in human disease. Nat Rev Genet. 2005;6:389–402.
117. Alcivar AA, Hake LE, Millette CF, Trasler JM, Hecht NB. Mitochondrial gene expression in male germ cells of the mouse. Dev Biol. 1989;135:263–71.
118. Solano A, Playan A, Lopez-Perez MJ, Montoya J. Genetic diseases of human mitochondrial DNA. Salud Publica Mex. 2001;43:1–11.
119. Guney AI, Javadova D, Kırac D, Ulucan K, Koc G, Ergec D, et al. Detection of Y chromosome microdeletions and mitochondrial DNA mutations in male infertility patients. Genet Mol Res. 2012;11:1039–48.
120. Carra E, Sangiorgi D, Gattuccio F, Rinaldi AM. Male infertility and mitochondrial DNA. Biochem Biophys Res Commun. 2004;322:333–9.
121. St John JC, Jokhi RP, Barratt CL. Men with oligoasthenoteratozoospermia harbour higher numbers of multiple mitochondrial DNA deletions in their spermatozoa, but individual deletions are not indicative of overall etiology. Mol Hum Reprod. 2001;7:103–11.
122. May-Panloup P, Chretien MF, Savagner F, Vasseur C, Jean M, Malthiery Y, et al. Increased sperm mitochondrial DNA content in male infertility. Hum Reprod. 2003;18:550–6.
123. Folgero T, Bertheussen K, Lindal S, Torbergsen T, Oian P. Mitochondrial disease and reduced sperm motility. Hum Reprod. 1993;8:1863–8.
124. Lestienne P, Reynier P, Chretien MF, Penisson-Besnier I, Malthièry Y, Rohmer V. Oligoasthenospermia associated with multiple mitochondrial DNA rearrangements. Mol Hum Reprod. 1997;3:811–4.
125. Hoshi K, Kasai T, Ogawa K, Mizuno K, Nagai S, Uchida Y, et al. Relationship between sperm mitochondrial membrane potential, sperm motility, and fertility potential. Asian J Androl. 2002;4:97–103.
126. Kao SH, Chao HT, Wei YH. Multiple deletions of mitochondrial DNA are associated with the decline of motility and fertility of human spermatozoa. Mol Hum Reprod. 1998;4:657–66.
127. Mundy AJ, Ryder TA, Edmonds DK. Asthenozoospermia and the human sperm mid-piece. Hum Reprod. 1995;10:116–9.
128. Hecht NB, Liem H, Kleene KC, Distel RJ, Ho SM. Maternal inheritance of the mouse mitochondrial genome is not mediated by a loss or gross alteration of the paternal mitochondrial DNA or by methylation of the oocyte mitochondrial DNA. Dev Biol. 1984;102:452–61.
129. Wallace DC. Diseases of the mitochondrial DNA. Annu Rev Biochem. 1992;61:1175–212.
130. Kumar V, Hassan MI, Tomar AK, Kashav T, Nautiyal J, Singh S, et al. Proteomic analysis of heparin-binding proteins fromhuman seminal plasma: a step towards identification of molecular markers of male fertility. J Biosci. 2009;34:899–908.
131. Folgero T, Torbergsen T, Oian P. The 3243 MELAS mutation in a pedigree with MERRF. Eur Neurol. 1995;35:168–71.
132. Kao SH, Chao HT, Wei YH. Mitochondrial deoxyribonucleic acid 4977-bp deletion is associated with diminished fertility and motility of human sperm. Biol Reprod. 1995;52:729–36.
133. Sampson MJ, Decker WK, Beaudet AL, Ruitenbeek W, Armstrong D, Hicks MJ, et al. Immotile sperm and infertility in mice lacking mitochondrial voltage-dependent anion channel type 3. J Biol Chem. 2001;276:39206–12.
134. Holyoake AJ, Sin IL, Benny PS, Sin FY. Association of a novel human mtDNA ATPase6 mutation with immature sperm cells. Andrologia. 1999;31:339–45.
135. O’Connell M, McClure N, Lewis SE. A comparison of mitochondrial and nuclear DNA status in testicular sperm from fertile men and those with obstructive azoospermia. Hum Reprod. 2002;17:1571–7.
136. Hosseinzadeh Colagar A, Karimi F. large scale deletions of the mitochondrial DNA in an asthenoterato and oligoasthenoterato-spermic men. Mitochondrial DNA. 2013. doi:10.3109/19401736.2013.796512.
137. St John JC, Jokhi RP, Barratt CLR. The impact of mitochondrial genetics on male infertility. Int J Androl. 2005;28:65–73.
138. Kumar R, Bhat A, Bamezai RN, Shamsi MB, Kumar R, Gupta NP, et al. Necessity of nuclear and mitochondrial genome analysis prior to ART/ICSI. Indian J Biochem Biophys. 2007;44:437–42.
139. Lambard S, Galeraud-Denis I, Martin G, Levy R, Chocat A, Carreau S. Analysis and significance of mRNA in human ejaculated sperm from normozoospermic donors: relationship to sperm motility and capacitation. Mol Hum Reprod. 2004;10:535–41.
140. Kumar R, Venkatesh S, Kumar M, Tanwar M, Shasmsi MB, Kumar R, et al. Oxidative stress and sperm mitochondrial DNA mutation in idiopathic oligoasthenozoospermic men. Indian J Biochem Biophys. 2009;46:172–7.
141. Thangaraj K, Joshi MB, Reddy AG, Rasalkar AA, Singh L. Sperm mitochondrial mutations as a cause of low sperm motility. J Androl. 2003;24:388–92.
142. Spiropoulos J, Turnbull DM, Chinnery PF. Can mitochondrial DNA mutations cause sperm dysfunction? Mol Hum Reprod. 2002;8:719–21.
143. Khosronezhad N, Hosseinzadeh Colagar A, Jorsarayi SGA. T26248G-transversion mutation in exon7 of the putative methyltransferase Nsun7 gene causes a change in protein folding associated with reduced sperm motility in asthenospermia men. Reprod Fertil and Dev. 2013. doi:10.1071/RD13371.
144. Rovio AT, Marchington DR, Donat S, Schuppe HC, Abel J, Fritsche E, et al. Mutations at the mitochondrial DNA polymerase (POLG) locus associated with male infertility. Nature Genet. 2001;29:261–2.
145. Chen SS, Chang LS, Chen HW, Wei YH. Polymorphisms of glutathione S-transferase M1 and male infertility in Taiwanese patients with varicocele. Hum Reprod. 2002;17:718–25.
146. St John JC, Sakkas D, Barrat CLR. A role for mitochondrial DNA in sperm survival. J Androl. 2000;21:189–99.
147. Shamsi MB, Kumar R, Bhatt A, Bamezai RNK, Kumar R, Gupta NP, et al. Mitochondrial DNA Mutations in etiopathogenesis of male infertility. Indian J Urol. 2008;24(2):150–4.
148. Rajender S, Avery K, Agarwal A. Epigenetics, spermatogenesis and male infertility. Mutat Res. 2011;727:62–71.
149. Rajender S, Agarwal A. Aberrant epigenetic modifications in male infertility. Open Reprod Sci J. 2011;3:57–64.
150. Dada R, Kumar M, Jesudasan R, Fernández LJ, Gosalvez J, Agarwal A. Epigenetics and its role in male infertility. J Assist Reprod Genet. 2012. doi:10.1007/s10815-012-9715-0.
151. Biermann K, Steger K. Epigenetics in male germ cells. J Androl. 2007;28:466–80.
152. Houshdaran S, Cortessis VK, Siegmund K, Yang A, Laird PW, Sokol RZ. Widespread epigenetic abnormalities suggest a broad DNA methylation erasure defect in abnormal human sperm. PLoS ONE. 2007;2:e1289.
153. Khazamipour N, Noruzinia M, Fatehmanesh P, Keyhanee M, Pujol P. MTHFR promoter hypermethylation in testicular biopsies of patients with non-obstructive azoospermia: the role of epigenetics in male infertility. Hum Reprod. 2009;24:2361–4.
154. Wu W, Shen O, Qin Y, Niu X, Lu C, Xia Y, et al. Idiopathic male infertility is strongly associated with aberrant promoter methylation of methylenetetrahydrofolate reductase (MTHFR). PLoS ONE. 2010;5:e13884.
155. Hammoud SS, Purwar J, Pflueger C, Cairns BR, Carrell DT. Alterations in sperm DNA methylation patterns at imprinted loci in two classes of infertility. Fertil Steril. 2010;94:1728–33.
156. Poplinski A, Tuttelmann F, Kanber D, Horsthemke B, Gromoll J. Idiopathic male infertility is strongly associated with aberrant methylation of MEST and IGF2/H19 ICR1. Int J Androl. 2010;33:642–9.
157. Boissonnas CC, Abdalaoui HE, Haelewyn V, Fauque P, Dupont JM, Gut I, et al. Specific epigenetic alterations of IGF2-H19 locus in spermatozoa from infertile men. Eur J Hum Genet. 2010;18:73–80.
158. Baarends WM, Wassenaar E, van der Laan R, Hoogerbrugge J, Sleddens-Linkels E, Hoeijmakers JH, et al. Silencing of unpaired chromatin and histone H2A ubiquitination in mammalian meiosis. Mol Cell Biol. 2005;25:1041–53.
159. Okada Y, Scott G, Ray MK, Mishina Y, Zhang Y. Histone demethylase JHDM2A is critical for Tnp1 and Prm1 transcription and spermatogenesis. Nature. 2007;450:119–23.
160. Miller D, Brinkworth M, Iles D. Paternal DNA packaging in spermatozoa: more than the sum of its parts? DNA, histones, protamines and epigenetics. Reproduction. 2010;139:287–301.
161. Oliva R. Protamines and male infertility. Hum Reprod Update. 2006;12:417–35.
162. Oliva R, Bazett-Jones D, Mezquita C, Dixon GH. Factors affecting nucleosome disassemble by protamines ‘in vitro’. Histone hyperacetylation and chromatin structure, time dependence, and the size of the sperm nuclear proteins. J Biol Chem. 1987;262:17016–25.
163. Oliva R, Dixon GH. Vertebrate protamine genes and the histone-to-protamine replacement reaction. Prog Nucleic Acid Res Mol Biol. 1991;40:25–94.
164. 2+/calmodulin-dependent protein kinase IV is expressed in spermatids and targeted to chromatin and the nuclear matrix. J Biol Chem. 2000;275:7994–9.
165. Oliva R, Mezquita C. Histone H4 hyperacetylation and rapid turnover of acetyl groups in transcriptionally inactive rooster testis spermatids. Nucleic Acids Res. 1982;10:8049–59.
166. Torregrosa N, Dominguez-Fandos D, Camejo MI, Shirley CR, Meistrich ML, Ballesca JL, et al. Protamine 2 precursors, protamine 1/protamine 2 ratio, DNA integrity and other sperm parameters in infertile patients. Hum Reprod. 2008;21:2084–9.
167. Aoki VW, Liu L, Carrell DT. Identification and evaluation of a novel sperm protamine abnormality in a population of infertile males. Hum Reprod. 2005;20:1298–306.
168. Faure AK, Pivot-Pajot C, Kerjean A, Hazzouri M, Pelletier R, Peoc’h M, et al. Misregulation of histone acetylation in Sertoli cell-only syndrome and testicular cancer. Mol Hum Reprod. 2003;9:757–63.
169. Sonnack V, Failing K, Bergmann M, Steger K. Expression of hyperacetylated histone H4 during normal and impaired human spermatogenesis. Andrologia. 2002;34:384–90.
170. Steilmann C, Cavalcanti MCO, Bartkuhn M, Pons-Kuhnemann J, Schuppe H, Weidner W, et al. The interaction of modified histones with the bromodomain testis-specific (BRDT) gene and its mRNA level in sperm of fertile donors and subfertile men. Reproduction. 2010;140:435–43.
171. Kobayashi H, Sato A, Otsu E, Hiura H, Tomatsu C, Utsunomiya T, et al. Aberrant DNA methylation of imprinted loci in sperm from oligospermic patients. Hum Mol Genet. 2007;16:2542–51.
172. Ostermeier GC, Miller D, Huntriss JD, Diamond MP, Krawetz SA. Reproductive biology: delivering spermatozoan RNA to the oocyte. Nature. 2004;429:154.
173. Li C, Zhou X. Gene transcripts in spermatozoa: Markers of male infertility. Clin Chim Acta. 2012;413:1035–8.
174. Li C, Zheng L, Wang C, Zhou X. Absence of nerve growth factor and comparison of tyrosine kinase receptor A levels in mature spermatozoa from oligoasthenozoospermic, asthenozoospermic and fertile men. Clin Chim Acta. 2010;411:1482–6.
175. Cai ZM, Gui YT. GuoX, Zhang LB, Wang H, Yu J. Low expression of glycoprotein subunit 130 in ejaculated spermatozoa from asthenozoospermic men. J Androl. 2006;27:645–52.
176. Guo X, Gui YT, Tang AF, Lu LH, Gao X, Cai ZM. Differential expression of VASA gene in ejaculated spermatozoa from normozoospermic men and patients with oligozoospermia. Asian J Androl. 2007;9:339–44.
177. Papaioanou MD, Nef S. MicroRNAs in the testis: building up male fertility. J Andeol. 2010;31:26–33.
178. Krawetz SA, Kruger A, Lalancette C, Tagett R, Anton E, Draghici S, et al. A survey of small RNAs in human sperm. Hum Reprod. 2011;26:3401–12.
179. Esquela-Kerscher A, Slack FJ. Oncomirs- microRNAs with a role in cancer. Nat Rev Cancer. 2006;6:259–69.
180. Bouhallier F, Allioli N, Lavial F, Chalmel F, Perrard M, Durand P, et al. Role of miR-34c microRNA in the late steps of spermatogenesis. RNA. 2010;16:720–31.
181. Belleannee C, Calvo E, Thimon V, Cyr DG, Legare C, Garneau L, et al. Role of MicroRNAs in Controlling Gene Expression in Different Segments of the Human Epididymis. PLoS ONE. 2012;7:1–13. e34996.
182. Bjork JK, Sandqvist A, Elsing AN, Kotaja N, Sistonen L. miR-18, a member of Oncomir-1, targets heat shock transcription factor 2 in spermatogenesis. Development. 2010;137:3177–84.
183. Barad O, Meiri E, Avniel A, Aharonov R, Barzilai A. B entwich I, E inav U, Gilad S, Hurban P, Karov Y, et al. MicroRNA expression detected by oligonucleotide microarrays: system establishment and expression profiling in human tissues. Genome Res. 2004;14:2486–94.
184. Yu ZR, Raabe T, Hecht NB. MicroRNA Mirn122a reduces expression of the posttranscriptionally regulated germ cell transition protein 2 (Tnp2) messenger RNA (mRNA) by mRNA cleavage. Biol Reprod. 2005;73:427–33.
185. Yu Z, Raabe T, Hecht NB. MicroRNA Mirn122a reduces expression of the posttranscriptionally regulated germ cell transi-tion protein 2 (Tnp2) messenger RNA (mRNA) by mRNA cleavage. Biol Reprod. 2005;73:427–33.
186. Novotny GW, Sonne SB, Nielsen JE, Jonstrup SP, Hansen MA, Skakkebaek NE, Rajpert-De Meyts E, Kjems J, Leffers H. Translational repression of E2F1 mRNA in carcinoma in situ and normal testis correlates with expression of the miR-17-92 cluster. Cell Death and Differentiation, 2007; 14879–882.
187. Govindaraju A, Uzun A, Robertson L, Atli MO, Kaya A, Topper E, et al. Dynamics of microRNAs in bull spermatozoa. Reprod Biol Endocrinol. 2012;10(82):1–10.
188. Yan N, Lu Y, Sun H, Tao D, Zhang S, Liu W, et al. A microarray for microRNA profiling in mouse testis tissues. Reproduction. 2007;134:73–9.
189. Lian C, Sun B, Niu S, Yang R, Liu B, Lu C, et al. A comparative profile of the microRNA transcriptome in immature and mature porcine testes using Solexa deep sequencing. FEBS J. 2012;279:964–75.
190. Voorhoeve PM, le Sage C, Schrier M, Gillis AJ, Stoop H, Nagel R, et al. D rost J, G riekspoor A et al. A genetic screen implicates miRNA-372 and miRNA-373 as oncogenes in testicular germ cell tumors. Adv Exp Med Biol. 2007;604:17–46.
191. Lim LP, Lau NC, Garrett-Engele P, Grimson A, Schelter JM, Castle J, et al. Microarray analysis shows that some microRNAs down-regulate large numbers of target mRNAs. Nature. 2005;433:769–73.
192. Sun W, Julie Li YS, Huang HD, Shyy JY, Chien S. MicroRNA: a master regulator of cellular processes for bioengi-neering systems. Annu Rev Biomed Eng. 2010;12:1–27.
193. Hayashi K, Chuva de Sousa Lopes SM, Kaneda M, Tang F, Hajkova P, Lao K, O’Carroll D, Das PP, Tarakhovsky A, Miska EA, Surani MA. MicroRNA biogenesis is required for mouse primordial germ cell development and spermatogenesis. PLoS One, 2008; 3:e1738.
194. Papaioannou MD, Pitetti JL, Ro S, Park C, Aubry F, Schaad O, et al. Sertoli cell DICER1 is essential for spermatogenesis in mice. Dev Biol. 2009;326:250–9.
195. Romero Y, Meikar O, Papaioannou MD, Conne B, Grey C, Weier M, et al. Dicer1 Depletion in Male Germ Cells Leads to Infertility Due to Cumulative Meiotic and Spermiogenic Defects. PLoS ONE. 2011;6:e25241.
196. Qin Y, Xia Y, Wu W, Han X, Lu C, Ji G, et al. Genetic variants in microRNA biogenesis pathway genes are associated with semen quality in a Han-Chinese population. Reprod BioMed Online. 2012;24:454–61.
197. Zhang C, Wang C, Chen X, Yang C, Li K, Wang J, et al. Expression profile of microRNAs in serum: a fingerprint for esophageal squamous cell carcinoma. Clin Chem. 2010;56:1871–9.
198. Huang S, Li H, Ding X, Xiong C. Presence and characterization of cell-free seminal RNA in healthy individuals: implications for noninvasive disease diagnosis and gene expression studies of the male reproductive system. Clin Chem. 2009;55:1967–76.
199. Wang C, Yang C, Chen X, Yao B, Yang C, Zhu C, et al. Altered Profile of Seminal Plasma MicroRNAs in the Molecular Diagnosis of Male Infertility. Clin Chem. 2011;57:1722–31.
200. Girard A, Sachidanandam R, Hannon GJ, Carmell MA. A germline-specific class of small RNAs binds mammalian Piwi proteins. Nature. 2006;442:199–202.
201. Klattenhoff C, Theurkauf W. Biogenesis and germline functions of piRNAs. Development, 135: 3–9.
202. He Z, Kokkinaki M, Pant D, Gallicano GI, Dym M. Small RNA molecules in the regulation of spermatogenesis. Reproduction. 2009;137:901–11.
203. Kuramochi-Miyagawa S. K imura T, Ijiri TW, Isobe T, Asada N, Fujita Y, Ikawa M, Iwai N, Okabe M, Deng W et al. Mili, a mammalian member of piwi family gene, is essential for spermatogenesis. Development. 2004;131:839–49.
204. Carmell MA, Girard A. van de K ant HJ, Bourchis D, Bestor TH, de Rooij DG, Hannon GJ. MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germline. Dev Cell. 2006;12:503–14.
205. Deng W, Lin H. miwi, a murine homolog of piwi, encodes a cytoplasmic protein essential for spermatogenesis. Dev Cell. 2002;2:819–30.
206. Xu M, You Y, Hunsicker P, Hori T, Small C, Griswold MD, et al. Mice deficient for a small cluster of Piwi-interacting RNAs implicate Piwi-interacting RNAs in transposon control. Biol Reprod. 2008;79(1):51–7.
207. du Plessis SS, Kashou AH, Benjamin DJ, Yadav SP, Agarwal A. Proteomics: a subcellular look at spermatozoa. Reprod Biol Endocrinol. 2011;9(36):1–12.
208. Aitken RJ, Baker MA. The role of proteomics in understanding sperm cell biology. Int J Androl. 2008;31:295–302.
209. Caron C, Govin J, Rousseaux S, Khochbin S. How to pack the genome for a safe trip. Prog Mol Subcell Biol. 2005;38:65–89.
210. Sassone-Corsi P. Unique chromatin remodeling and transcriptional regulation in spermatogenesis. Science. 2002;296:2176–8.
211. Li HY, Zhang H. Proteome analysis for profiling infertility markers in male mouse sperm after carbon ion radiation. Toxicol. 2013;306(5):85–92.
212. Sharma R, Agarwal A, Mohanty G, Jesudasan R, Gopalan B, Willard B, et al. Functional proteomic analysis of seminal plasma proteins in men with various semen parameters. Reprod Biol Endocrinol. 2013;11:11–38.
213. Milardi D, Grande G, Vincenzoni F, Messana I, Pontecorvi A, De Marinis L, et al. Proteomic approach in the identification of fertility pattern in seminal plasma of fertile men. Fertil Steril. 2012;97:67–73.
214. Siva AB, Kameshwari DB, Singh V, Pavani K, Sundaram CS, Rangaraj N, et al. Proteomics-based study on asthenozoospermia: differential expression of proteasome alpha complex. Mol Hum Reprod. 2010;16:452–62.
215. Kashou AH, Benjamin DJ, Agarwal A, du Plessis SS. The Advent of Sperm Proteomics has Arrived. Open Reprod Sci J. 2011;3:92–7.
216. Sharma A, Agarwal A, Mohanty G, Hamada AJ, Gopalan B, Willard B, et al. Proteomic analysis of human spermatozoa proteins with oxidative stress. Reproductive Biology and Endocrinology. 2013;11(48):1–18.
217. Thacker S, Yadav SP, Sharma RK, Kashou A, Willard B, Zhang D, et al. Evaluation of Sperm Proteins in Infertile Men: A Proteomic Approach. Fertil Steril. 2011;95:2745–8.
218. Martinez-Heredia J, de Mateo S, Vidal-Taboada JM, Ballesca JL, Oliva R. Identification of proteomic differences in asthenozoospermic sperm samples. Hum Reprod. 2008;23:783–91.
219. Zhao L, Burkin HR, Shi X, Li L, Reim K, Miller DJ. Complexin I is required for mammalian sperm acrosomal exocytosis. Dev Biol. 2007;309:236–44.
220. de Mateo S, Martinez-Heredia J, Estanyol JM, Dominguez-Fandos D, Vidal-Taboada JM, Ballesca JL, et al. Marked correlations in protein expression identified by proteomic analysis of human spermatozoa. Proteomics. 2007;7:4264–77.
221. Agarwal A. Relationship between oxidative stress, varicocele and infertility: a meta-analysis. Reprod BioMed Online. 2006;12:630–3.
222. Vaidya D. The hormonal assessment of the infertile male. In: A Publication of the Hope Infertility Clinic. 2006; Available at: education. vsnl.com/hic/hic.html.
223. Askienazy-Elbhar M. Male genital tract infection: the point of view of the bacteriologist. Gynecol Obstet Fertil. 2005;33(9):691–7.
224. Peterson CM. Human reproduction: clinical, pathologic and pharmacologic correlations. In: Human Reproduction. 2006; Available at: library.med.utah.edu/kw/human_reprod/seminars/seminar2B.html.
225. Collins JA, Burrows EA, Yeo J, YoungLai EV. Frequency and predictive value of antisperm antibodies among infertile couples. Hum Reprod. 1993;8:592–8.
226. Ambasudhan R, Singh K, Agarwal JK, Singh SK, Khanna A, Sah RK, et al. Idiopathic cases of male infertility from a region in India show low incidence of Y-chromosome microdeletions. J Biosci. 2003;28:605–12.
227. Hwang SK, Makita Y, Kurahashi H, Cho YW, Hirose S. Autosomal dominant nocturnal frontal lobe epilepsy: a genotypic comparative study of Japanese and Korean families carrying the CHRNA4 Ser284Leu mutation. J Hum Genet. 2011;56:609–12.