Inactivation of a GAL4-like transcription factor improves cell fitness and product yield in glycoengineered Pichia pastoris strains

Applied and Environmental Microbiology

Volumn 81, Issue 1, 2015, Pages 260-271

  Jiang, Bo ^a,b   Argyros, Rebecca ^a   Bukowski, John ^a,c   Nelson, Stephanie ^a   Sharkey, Nathan ^a   Kim, Sehoon ^a   Copeland, Victoria ^a,d   Davidson, Robert C ^a   Chen, Ronghua ^b,e   Zhuang, Jun ^b   Sethuraman, Natarajan ^a   Stadheim, Terrance A ^a  

^a GlycoFi Incorporated   (United States)

^b MERCK RESEARCH LABORATORIES   (United States)

^c Adimab LLC   (United States)

^d ELI LILLY AND COMPANY   (United States)

^e R and D China Co Ltd   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; CELLS; CYTOLOGY; FERMENTATION; GENE THERAPY; GENES; HEALTH; MANUFACTURE; MONOCLONAL ANTIBODIES; PROTEINS; TRANSCRIPTION;

GENETIC MODIFICATIONS; GLYCOENGINEERED PICHIA PASTORIS; INDUSTRIAL FERMENTATION; PICHIA PASTORIS; PRODUCTION STRAIN; THERAPEUTIC PROTEIN; THERMAL TOLERANCE; TRANSCRIPTIONAL ACTIVATORS;

YEAST;

FERMENTATION; GENE EXPRESSION; GENETIC ANALYSIS; GENETIC ENGINEERING; GENETIC VARIATION; GERM CELL; INDUSTRIAL PRODUCTION; MUTATION; PROTEIN; YEAST;

PICHIA; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

DNA BINDING PROTEIN; GAL4 PROTEIN, S CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEIN; TRANSCRIPTION FACTOR;

GENE DELETION; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETICS; GLYCOSYLATION; GROWTH, DEVELOPMENT AND AGING; METABOLIC ENGINEERING; METABOLISM; MICROBIAL VIABILITY; PICHIA; SEQUENCE HOMOLOGY; VIRULENCE;

DNA-BINDING PROTEINS; GENE DELETION; GENE EXPRESSION REGULATION, FUNGAL; GLYCOSYLATION; METABOLIC ENGINEERING; MICROBIAL VIABILITY; PICHIA; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE HOMOLOGY; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC; VIRULENCE;

EID: 84917678605     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02619-14     Document Type: Article

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