GinsenosideRg5 induces apoptosis and DNA damage in human cervical cancer cells

Molecular Medicine Reports

Volumn 11, Issue 2, 2015, Pages 940-946

  Liang, Li Dan ^a   He, Tao ^a   Du, Ting Wei ^a   Fan, Yong Gang ^a   Chen, Dian Sen ^a   Wang, Yan ^a  

^a Hennan University of Science and Technology   (China)

Author keywords

Apoptosis; Cervical cancer; DNA damage; GinsenosideRg5

Indexed keywords

PANAX GINSENG;

ANTINEOPLASTIC AGENT; GINSENOSIDE; GINSENOSIDE RG5;

APOPTOSIS; CELL SURVIVAL; CHEMISTRY; COMET ASSAY; DNA DAMAGE; DNA FRAGMENTATION; DRUG EFFECTS; FEMALE; HELA CELL LINE; HUMAN; PANAX; PATHOLOGY; TUMOR CELL LINE; UTERINE CERVIX TUMOR;

ANTINEOPLASTIC AGENTS, PHYTOGENIC; APOPTOSIS; CELL LINE, TUMOR; CELL SURVIVAL; COMET ASSAY; DNA DAMAGE; DNA FRAGMENTATION; FEMALE; GINSENOSIDES; HELA CELLS; HUMANS; PANAX; UTERINE CERVICAL NEOPLASMS;

EID: 84916220553     PISSN: 17912997     EISSN: 17913004     Source Type: Journal    
DOI: 10.3892/mmr.2014.2821     Document Type: Article

References (42)

1. Saslow D, Castle PE, Cox JT, et al: American Cancer Society Guideline for human papillomavirus (HPV) vaccine use to prevent cervical cancer and its precursors. CA Cancer J Clin 57: 7-28, 2007.
2. Steben M and Duarte-Franco E: Human papillomavirus infection: epidemiology and pathophysiology. Gynecol Oncol 107: S2-S5, 2007.
3. Long HJ 3rd: Management of metastatic cervical cancer: review of the literature. J Clin Oncol 25: 2966-2974, 2007.
4. Brinkman JA, Caffrey AS, Muderspach LI, Roman LD and Kast WM: Inhibitory effect of ginsenoside Rg5 and its metabolite ginsenoside Rh3 in an oxazolone induced mouse chronic dermatitis model. Eur J Gynaecol Oncol 26: 129 142, 2005.
5. Leitao MM Jr and Chi DS: Recurrent cervical cancer. Curr Treat Options Oncol 3: 105-111, 2002.
6. Yang J, Li J, Sun M and Chen K: Studies of traditional Chinese medicine monomer on HeLa cell of cervical cancer. Pak J Pharm Sci 27 (4 Suppl): 1063-1068, 2014.
7. Cohen I, Tagliaferri M and Tripathy D: Traditional Chinese medicine in the treatment of breast cancer. Semin Oncol 29: 563-574, 2002.
8. Li X, Yang G, Li X, et al: Traditional Chinese medicine in cancer care: a review of controlled clinical studies published in Chinese. PloS One 8: e60338, 2013
9. Yang G, Li X, Li X, et al: Traditional chinese medicine in cancer care: a review of case series published in the chinese literature. Evid Based Complement Alternat Med 2012: 7510-7546, 2012.
10. Liu J, Li X, Liu J, Ma L, Li X and Fønnebø V: Traditional Chinese medicine in cancer care: a review of case reports published in Chinese literature. Forsch Komplementarmed 18: 257-263, 2011.
11. Kwon SW, Han SB, Park IH, Kim JM, Park MK and Park JH: Liquid chromatographic determination of less polar ginsenosides in processed ginseng. J Chromatogr A 921: 335-339, 2001.
12. Kim SN, Ha YW, Shin H, Son SH, Wu SJ and Kim YS: Simultaneous quantification of 14 ginsenosides in Panax ginseng C.A. Meyer (Korean red ginseng) by HPLC-ELSD and its application to quality control. J Pharm Biomed Anal 45: 164-170, 2007.
13. Yun TK, Lee YS, Lee YH, Kim SI and Yun HY: Anticarcinogenic effect of Panax ginseng C.A. Meyer and identification of active compounds. J Korean Med Sci 16: S6-S18, 2001.
14. Kang KS, Kim HY, Baek SH, et al: Study on the hydroxyl radical scavenging activity changes of ginseng and ginsenoside-Rb2 by heat processing. Biol Pharm Bull 30: 724-728, 2007.
15. Nag SA, Qin JJ, Wang W, et al: Ginsenosides as anticancer agents: In vitro and in vivo activities, structure-activity relationships, and molecular mechanisms of action. Front Pharmacol 3: 25, 2012.
16. Lee KY, Lee YH, Kim SI, Park JH and Lee SK: Ginsenoside-Rg5 suppresses cyclin E-dependent protein kinase activity via up-regulating p21Cip/WAF1 and down-regulating cyclin E in SK-HEP-1 cells. Anticancer Res 17: 1067-1072, 1997.
17. Shin YW, Bae EA and Kim DH: Inhibitory effect of ginsenoside Rg5 and its metabolite ginsenoside Rh3 in an oxazolone-induced mouse chronic dermatitis model. Arch Pharm Res 29: 685-690, 2006.
18. Kim TW, Joh EH, Kim B and Kim DH: Ginsenoside Rg5 ameliorates lung inflammation in mice by inhibiting the binding of LPS to toll-like receptor-4 on macrophages. Int Immunopharmacol 12: 110-116, 2012.
19. Kim EJ, Jung IH, Van Le TK, Jeong JJ, Kim NJ and Kim DH: Ginsenosides Rg5 and Rh3 protect scopolamine-induced memory deficits in mice. J Ethnopharmacol 146: 294-299, 2013.
20. Lee YY, Park JS, Jung JS, Kim DH and Kim HS: Anti-inflammatory effect of ginsenoside Rg5 in lipopolysaccharide-stimulated BV2 microglial cells. Int J Mol Sci 14: 9820-9833, 2013.
21. Kawai K, Liu SX, Tyurin VA, et al: Antioxidant and antiapoptotic function of metallothioneins in HL-60 cells challenged with copper nitrilotriacetate. Chem Res Toxicol 13: 1275-1286, 2000.
22. Wu SB, Su JJ, Sun LH, et al: Triterpenoids and steroids from the fruits of Melia toosendan and their cytotoxic effects on two human cancer cell lines. J Natl Prod 73: 1898-1906, 2010.
23. Nakadai A, Li Q and Kawada T: Chlorpyrifos induces apoptosis in human monocyte cell line U937. Toxicology 224: 202-209, 2006.
24. Guanggang X, Diqiu L, Jianzhong Y, et al: Carbamate insecticide methomyl confers cytotoxicity through DNA damage induction. Food Chem Toxicol 53: 352-358, 2013.
25. Zhang YH, Li HD, Li B, Jiang SD and Jiang LS: Ginsenoside Rg3 induces DNA damage in human osteosarcoma cells and reduces MNNG-induced DNA damage and apoptosis in normal human cells. Oncol Rep 31: 919-925, 2014.
26. Wu GS, Lu JJ, Guo JJ, et al: Ganoderic acid DM, a natural triterpenoid, induces DNA damage, G1 cell cycle arrest and apoptosis in human breast cancer cells. Fitoterapia 83: 408-414, 2012.
27. Sokolov MV, Smilenov LB, Hall EJ, Panyutin IG, Bonner WM and Sedelnikova OA: Ionizing radiation induces DNA double-strand breaks in bystander primary human fibroblasts. Oncogene 24: 7257-7265, 2005.
28. Parry MC, Bhabra G, Sood A, et al: Thresholds for indirect DNA damage across cellular barriers for orthopaedic biomaterials. Biomaterials 31: 4477-4483, 2010.
29. Toh DF, Patel DN, Chan EC, Teo A, Neo SY and Koh HL: Anti-proliferative effects of raw and steamed extracts of Panax notoginseng and its ginsenoside constituents on human liver cancer cells. Chin Med 6: 4, 2011.
30. Yue PY, Mak NK, Cheng YK, et al: Pharmacogenomics and the Yin/Yang actions of ginseng: anti-tumor, angiomodulating and steroid-like activities of ginsenosides. Chin Med 2: 6, 2007.
31. Bras M, Queenan B and Susin SA: Programmed cell death via mitochondria: different modes of dying. Biochemistry (Mosc) 70: 231-239, 2005.
32. Broker LE, Kruyt FA and Giaccone G: Cell death independent of caspases: a review. Clin Cancer Res 11: 3155-3162, 2005.
33. Edinger AL and Thompson CB: Death by design: apoptosis, necrosis and autophagy. Curr Opin Cell Biol 16: 663-669, 2004.
34. Kim HS, Lee EH, Ko SR, Choi KJ, Park JH and Im DS: Effects of ginsenosides Rg3 and Rh2 on the proliferation of prostate cancer cells. Arch Pharm Res 27: 429-435, 2004.
35. Park HM, Kim SJ, Kim JS and Kang HS: Reactive oxygen species mediated ginsenoside Rg3- and Rh2-induced apoptosis in hepatoma cells through mitochondrial signaling pathways. Food Chem Toxicol 50: 2736-2741, 2012.
36. Li B, Zhao J, Wang CZ, et al: Ginsenoside Rh2 induces apoptosis and paraptosis-like cell death in colorectal cancer cells through activation of p53. Cancer Lett 301: 185-192, 2011.
37. Kim SE, Lee YH, Park JH and Lee SK: Ginsenoside-Rs4, a new type of ginseng saponin concurrently induces apoptosis and selectively elevates protein levels of p53 and p21WAF1 in human hepatoma SK-HEP-1 cells. Eur J Cancer 35: 507-511, 1999.
38. Oh SH and Lee BH: A ginseng saponin metabolite-induced apoptosis in HepG2 cells involves a mitochondria-mediated pathway and its downstream caspase-8 activation and Bid cleavage. Toxicol Appl Pharmacol 194: 221-229, 2004.
39. Robertson JD and Orrenius S: Role of mitochondria in toxic cell death. Toxicology 181-182: 491-496, 2002.
40. Poon PY, Kwok HH, Yue PY, et al: Cytoprotective effect of 20S-Rg3 on benzo[a]pyrene-induced DNA damage. Drug Metab Dispos 40: 120-129, 2012.
41. Cotelle S and Férard JF: Comet assay in genetic ecotoxicology: a review. Environ Mol Mutagen 34: 246-255, 1999.
42. Yu Y, Zhu W, Diao H, Zhou C, Chen FF and Yang J: A comparative study of using comet assay and gammaH2AX foci formation in the detection of N-methyl-N'-nitro-N-nitrosoguanidine-induced DNA damage. Toxicology In Vitro 20: 959-965, 2006.