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When zinc(ii) is added to MBD (Fig. S33 in ESI) no appreciable increase in emission is observed confirming that protonation results from CCD. Having the morpholinyl group is important as replacing it with a dimethyl amino group results in a false turn "on" with zinc(ii) This value is comparable to those obtained with various zinc(ii) sensors in organic solvents
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- leads to a side (addition) reaction with the imine (Fig. S23 in ESI). In order to obtain a cleaner transformation we used TREN as the demetalation agent. This yielded a clean and reversible catalysis (Fig. S24 in ESI); however, the partial consumption of the released proton by TREN upon reintroduction of zinc(ii) to the mixture, significantly slowed down the reaction rate (i.e., hydrolysis completes in 6 h). This also means that now we have a larger turnover number, because less protons are available for catalysis
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