Analysis of the Staphylococcus aureus capsule biosynthesis pathway in vitro: Characterization of the UDP-GlcNAc C6 dehydratases CapD and CapE and identification of enzyme inhibitors

International Journal of Medical Microbiology

Volumn 304, Issue 8, 2014, Pages 958-969

  Li, Wenjin ^a   Ulm, Hannah ^a   Rausch, Marvin ^a   Li, Xue ^b,c   O'Riordan, Katie ^b   Lee, Jean C ^b   Schneider, Tanja ^a,d   Müller, Christa E ^a  

^a UNIVERSITY OF BONN   (Germany)

^b HARVARD MEDICAL SCHOOL   (United States)

^c INSTITUTE OF MEDICINAL BIOTECHNOLOGY   (China)

^d UNIVERSITY OF COLOGNE   (Germany)

Author keywords

Antibiotics; Capillary electrophoresis; Capsule; Cell wall; Enzyme inhibitors; Enzyme kinetics; Staphylococcus aureus

Indexed keywords

AMPICILLIN; BACTERIAL PROTEIN; ENZYME INHIBITOR; HEXADIMETHRINE BROMIDE; MEMBRANE PROTEIN; PENTAPEPTIDE; PROTEIN CAPD; PROTEIN CAPE; SURAMIN; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE; ANTIINFECTIVE AGENT; HYDROLYASE; WBPM PROTEIN, PSEUDOMONAS AERUGINOSA;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACTERIAL CAPSULE; BACTERIAL CELL WALL; BIOSYNTHESIS; CAPILLARY ELECTROPHORESIS; COMPETITIVE INHIBITION; DRUG IDENTIFICATION; ENZYME ACTIVITY; ENZYME INHIBITION ASSAY; ENZYME KINETICS; HEATING; HIGH THROUGHPUT SCREENING; IN VITRO STUDY; LIMIT OF DETECTION; MICELLAR ELECTROKINETIC CHROMATOGRAPHY; MINIMUM INHIBITORY CONCENTRATION; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE BINDING SITE; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; REGULATORY MECHANISM; SEROTYPE; STAPHYLOCOCCUS AUREUS; STEREOISOMERISM; ULTRACENTRIFUGATION; ANTAGONISTS AND INHIBITORS; ENZYMOLOGY; GENETICS; IC50; ISOLATION AND PURIFICATION; KINETICS; METABOLISM;

AMPICILLIN; ANTI-BACTERIAL AGENTS; BACTERIAL CAPSULES; BACTERIAL PROTEINS; BIOSYNTHETIC PATHWAYS; ENZYME INHIBITORS; HYDRO-LYASES; INHIBITORY CONCENTRATION 50; KINETICS; STAPHYLOCOCCUS AUREUS; SURAMIN;

EID: 84911999672     PISSN: 14384221     EISSN: 16180607     Source Type: Journal    
DOI: 10.1016/j.ijmm.2014.06.002     Document Type: Article

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