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3543141065
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note
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All experiments were performed with a Hewlett-Packard 3D capillary electrophoresis system equipped with a capillary (50-μm inside diameter, 56 cm to the detector, and 64.5 cm total length) thermostated at 20°C. Burning off a 3-mm portion of the polyimide capillary coating formed the detection window.
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15
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3543051073
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note
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r are the migration times of the marker and analyte, respectively.
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16
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3543147068
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note
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The trend of Eq. 2 was not verified for the higher k values, because accurate determination of k when values are very high (>100) is difficult. This is because elution occurs rapidly (peak compression) and no separation occurs without the addition of aqueous-phase modifiers to the BGS.
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17
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3543059234
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note
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With 2-m-long capillaries and applied voltage at -30 kV, migration times were more than 1 hour.
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18
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3543060447
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Sweeping enhancement factor = peak height obtained with sweeping/peak height obtained with usual injection (1 or 2 s at 50 millibars). Supplementary material is available at www.sciencemag.org/feature/data/983374.shl.
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19
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3543087274
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note
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Conditions: injected length of S, 42 cm; other conditions are the same as in Fig. 2; equation of the line, log(ppb) = (slope) × log(mAU) + y intercept; calibration concentration range, 10 to 100 ppb; limit of detection, signal/noise ratio = 3; %RSD (n = 7).
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20
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3543128194
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note
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We thank Drs. K. Otsuka and N. Matsubara for support. J.P.Q. is also grateful to the Ministry of Education, Science, Culture, and Sports, Japan (Monbusho) for a Ph.D. scholarship. Supported in part by grants-in-aid for scientific research (nos. 07554040 and 09304071) from Monbusho.
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