Exceeding 5000-fold concentration of dilute analytes in micellar electrokinetic chromatography

Science

Volumn 282, Issue 5388, 1998, Pages 465-468

  Quirino, Joselito P ^a   Terabe, Shigeru ^a  

^a UNIVERSITY OF HYOGO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

HERBICIDE; LAKE WATER;

ELECTRIC FIELD; LIGHT ABSORPTION; MICELLAR ELECTROKINETIC CHROMATOGRAPHY; PRIORITY JOURNAL; REVIEW; WATER CONTAMINATION;

CHROMATOGRAPHY, MICELLAR ELECTROKINETIC CAPILLARY; HYDROGEN-ION CONCENTRATION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SODIUM DODECYL SULFATE;

EID: 0032538519     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.282.5388.465     Document Type: Review

References (20)

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6. Z. Liu et al., J. Chromatogr. A 673, 125 (1994); K. R. Nielsen and J. P. Foley, ibid. 686, 283 (1994); J. P. Quirino and S. Terabe, ibid. 781, 119 (1997); ibid. 791, 255 (1997); ibid. 798, 251 (1998); Anal. Chem. 70, 149 (1998); ibid., p. 1893; J. P. Quirino, K. Otsuka, S. Terabe J. Chromatogr. B 714, 29 (1998).
7. Z. Liu et al., J. Chromatogr. A 673, 125 (1994); K. R. Nielsen and J. P. Foley, ibid. 686, 283 (1994); J. P. Quirino and S. Terabe, ibid. 781, 119 (1997); ibid. 791, 255 (1997); ibid. 798, 251 (1998); Anal. Chem. 70, 149 (1998); ibid., p. 1893; J. P. Quirino, K. Otsuka, S. Terabe J. Chromatogr. B 714, 29 (1998).
8. Z. Liu et al., J. Chromatogr. A 673, 125 (1994); K. R. Nielsen and J. P. Foley, ibid. 686, 283 (1994); J. P. Quirino and S. Terabe, ibid. 781, 119 (1997); ibid. 791, 255 (1997); ibid. 798, 251 (1998); Anal. Chem. 70, 149 (1998); ibid., p. 1893; J. P. Quirino, K. Otsuka, S. Terabe J. Chromatogr. B 714, 29 (1998).
9. Z. Liu et al., J. Chromatogr. A 673, 125 (1994); K. R. Nielsen and J. P. Foley, ibid. 686, 283 (1994); J. P. Quirino and S. Terabe, ibid. 781, 119 (1997); ibid. 791, 255 (1997); ibid. 798, 251 (1998); Anal. Chem. 70, 149 (1998); ibid., p. 1893; J. P. Quirino, K. Otsuka, S. Terabe J. Chromatogr. B 714, 29 (1998).
10. Z. Liu et al., J. Chromatogr. A 673, 125 (1994); K. R. Nielsen and J. P. Foley, ibid. 686, 283 (1994); J. P. Quirino and S. Terabe, ibid. 781, 119 (1997); ibid. 791, 255 (1997); ibid. 798, 251 (1998); Anal. Chem. 70, 149 (1998); ibid., p. 1893; J. P. Quirino, K. Otsuka, S. Terabe J. Chromatogr. B 714, 29 (1998).
11. Z. Liu et al., J. Chromatogr. A 673, 125 (1994); K. R. Nielsen and J. P. Foley, ibid. 686, 283 (1994); J. P. Quirino and S. Terabe, ibid. 781, 119 (1997); ibid. 791, 255 (1997); ibid. 798, 251 (1998); Anal. Chem. 70, 149 (1998); ibid., p. 1893; J. P. Quirino, K. Otsuka, S. Terabe J. Chromatogr. B 714, 29 (1998).
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14. All experiments were performed with a Hewlett-Packard 3D capillary electrophoresis system equipped with a capillary (50-μm inside diameter, 56 cm to the detector, and 64.5 cm total length) thermostated at 20°C. Burning off a 3-mm portion of the polyimide capillary coating formed the detection window.
15. r are the migration times of the marker and analyte, respectively.
16. The trend of Eq. 2 was not verified for the higher k values, because accurate determination of k when values are very high (>100) is difficult. This is because elution occurs rapidly (peak compression) and no separation occurs without the addition of aqueous-phase modifiers to the BGS.
17. With 2-m-long capillaries and applied voltage at -30 kV, migration times were more than 1 hour.
18. Sweeping enhancement factor = peak height obtained with sweeping/peak height obtained with usual injection (1 or 2 s at 50 millibars). Supplementary material is available at www.sciencemag.org/feature/data/983374.shl.
19. Conditions: injected length of S, 42 cm; other conditions are the same as in Fig. 2; equation of the line, log(ppb) = (slope) × log(mAU) + y intercept; calibration concentration range, 10 to 100 ppb; limit of detection, signal/noise ratio = 3; %RSD (n = 7).
20. We thank Drs. K. Otsuka and N. Matsubara for support. J.P.Q. is also grateful to the Ministry of Education, Science, Culture, and Sports, Japan (Monbusho) for a Ph.D. scholarship. Supported in part by grants-in-aid for scientific research (nos. 07554040 and 09304071) from Monbusho.