Staphylococcus aureus cap5P encodes a UDP-N-acetylglucosamine 2- epimerase with functional redundancy

Journal of Bacteriology

Volumn 181, Issue 16, 1999, Pages 4818-4824

  Kiser, Kevin B ^a   Bhasin, Navneet ^a,b   Deng, Lingyi ^a,c   Lee, Jean C ^a  

^a BRIGHAM AND WOMEN S HOSPITAL   (United States)

^b BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c BOSTON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EPIMERASE; GENE PRODUCT; POLYSACCHARIDE; PROTEIN CAP5P; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE N ACETYLGLUCOSAMINE; URIDINE DIPHOSPHATE N ACETYLGLUCOSAMINE 2 EPIMERASE;

ARTICLE; BACTERIAL GENE; BACTERIAL MEMBRANE; ESCHERICHIA COLI; GENE CLUSTER; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN PURIFICATION; SEQUENCE HOMOLOGY; STAPHYLOCOCCUS AUREUS;

EID: 0032816695     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.16.4818-4824.1999     Document Type: Article

References (28)

1. Albus, A., R. D. Arbeit, and J. C. Lee. 1991. Virulence of Staphylococcus aureus mutants altered in type 5 capsule production. Infect. Immun. 59: 1008-1014.
2. Bhasin, N., A. Albus, F. Michon, P. J. Livolsi, J.-S. Park, and J. C. Lee. 1998. Identification of a gene essential for O-acetylation of the Staphylococcus aureus type 5 capsular polysaccharide. Mol. Microbiol. 27:9-21.
3. Bradford, M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
4. Edman, P. 1950. Method for determination of the amino acid sequence in peptides. Acta Chem. Scand. 4:283-293.
5. Institute of Genomic Research Website. 1999, copyright date. 7 April 1999, revision date. Sequences. [Online.] The Institute for Genomic Research. http://www.tigr.org. [5 July 1999, last date accessed.]
6. Kasatiya, S. S., and J. N. Baldwin. 1967. Nature of the determinant of tetracycline resistance in Staphylococcus aureus. Can. J. Microbiol. 13:1079-1086.
7. Kawamura, T., M. Kimura, S. Yamamori, and E. Ito. 1978. Enzymatic formation of uridine diphosphate N-acetyl-D-mannosamine. J. Biol. Chem. 253:3595-3601.
8. Kaya, S., K. Yokoyama, Y. Araki, and E. Ito. 1984. N-Acetylmannosaminyl (1→4) N-acetylglucosamine, a linkage unit between glycerol teichoic acid and peptidoglycan in cell walls of several Bacillus strains. J. Bacteriol. 158: 990-996.
9. Keenleyside, W. J., and C. Whitfield. 1996. A novel pathway for O-polysaccharide biosynthesis in Salmonella enterica serovar Borreze. J. Biol. Chem. 271:28581-28592.
10. Kiser, K. B., and J. C. Lee. 1998. Staphylococcus aureus cap5O and cap5P genes functionally complement mutations affecting enterobacterial common-antigen biosynthesis in Escherichia coli. J. Bacteriol. 180:403-406.
11. Kojima, N., Y, Araki, and E. Ito. 1983. Structure of linkage region between ribitol teichoic acid and peptidoglycan in cell walls of Staphylococcus aureus H. J. Biol. Chem. 258:9043-9045.
12. Kuhn, H.-M., U. Meier-Dieter, and H. Mayer. 1988. ECA, the enterobacterial common antigen. FEMS Microbiol. Rev. 54:195-222.
13. Lee, J. C. 1995. Electrotransformation of staphylococci. Methods Mol. Biol. 47:209-216.
14. Lee, J. C., M. J. Betley, C. A. Hopkins, N. E. Perez, and G. B. Pier. 1987. Virulence studies, in mice, of transposon-induced mutants of Staphylococcus aureus differing in capsule size. J. Infect. Dis. 156:741-750.
15. Lee, J. C., and C. Y. Lee. 1999. Capsular polysaccharides of Staphylococcus aureus, p. 185-205. In J. B. Goldberg (ed.), Genetics of bacterial polysaccharides. CRC Press, Inc., Boca Raton, Fla.
16. Lee, J. C., S. Takeda, P. J. Livolsi, and L. C. Paolelti. 1993. Effects of in vitro and in vivo growth conditions on expression of type-8 capsular polysaccharide by Staphylococcus aureus. Infect. Immun. 61:1853-1858.
17. Macrina, F. L., R. P. Evans, J. A. Tobian, D. L. Hartley, D. B. Clewell, and K. R. Jones. 1983. Novel shuttle plasmid vehicles for Escherichia-Streptococcus transgeneric cloning. Gene 25:145-150.
18. Meier-Dieter, U., R. Starman, K. Barr, H. Mayer, and P. D. Rick. 1990. Biosynthesis of enterobacterial common antigen in Escherichia coli. J. Biol. Chem. 265:13490-13497.
19. Morona, J. K., R. Morona, and J. C. Paton. 1997. Characterization of the locus encoding the Streptococcus pneumoniae type 19F capsular polysaccharide biosynthetic pathway. Mol. Microbiol. 23:751-763.
20. O'Connell, C., P. A. Pattee, and T. J. Foster. 1993. Sequence and mapping of the aroA gene of Staphylococcus aureus 8325-4. J. Gen. Microbiol. 139:1449-1460.
21. Peng, H.-L., R. P. Novick, B. Kreiswirth, J. Kornblum, and P. Schlievert. 1988. Cloning, characterization, and sequencing of an accessory gene regulator (agr) in Staphylococcus aureus. J. Bacteriol. 170:4365-4372.
22. Sala, R. F., P. M. Morgan, and M. E. Tanner. 1996. Enzymatic formation and release of a stable glycal intermediate: the mechanism of the reaction catalyzed by UDP-N-acetylglucosamine 2-epimerase. J. Am. Chem. Soc. 118: 3033-3034.
23. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
24. Sau, S., N. Bhasin, E. R. Wann, J. C. Lee, T. J. Foster, and C. Y. Lee. 1997. The Staphylococcus aureus allelic genetic loci for serotype 5 and 8 capsule expression contain the type-specific genes flanked by common genes. Microbiology 143:2395-2405.
25. Sau, S., J. Sun, and C. Y. Lee. 1997. Molecular characterization and transcriptional analysis of type 8 capsule genes in Staphylococcus aureus. J. Bacteriol. 179:1614-1621.
26. Sawardeker, J. S., J. H. Sloneker, and A. Jeanes. 1965. Quantitative determination of monosaccharides as their alditol acetates by gas liquid chromatography. Anal. Chem. 37:1602-1604.
27. Soldo, B., V. Lazarevic, P. Margot, and D. Karamata. 1993. Sequencing and analysis of the divergon comprising gtaB, the structural gene of UDP-glucose pyrophosphorylase of Bacillus subtilis 168. J. Gen. Microbiol. 139:3185-3195.
28. Yoneyama, T., Y. Koike, H. Arakawa, K. Yokoyama, Y. Sasaki, T. Kawamura, Y. Araki, E. Ito, and S. Takao. 1982. Distribution of mannosamine and mannosaminuronic acid among cell walls of Bacillus species. J. Bacteriol. 149:15-21.