Cellular reporter systems for high-throughput screening of interactions between bioactive matrices and human mesenchymal stromal cells

Tissue Engineering - Part C: Methods

Volumn 20, Issue 10, 2014, Pages 828-837

  Duryagina, Regina ^a   Anastassiadis, Konstantinos ^b   Maitz, Manfred F ^c   Gramm, Stefan ^c   Schneider, Susan ^b   Wobus, Manja ^a   Thieme, Sebastian ^a   Brenner, Sebastian ^a   Werner, Carsten ^c   Bornhaüser, Martin ^a,d  

^a UNIVERSITY HOSPITAL CARL GUSTAV CARUS   (Germany)

^b DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^c LEIBNIZ INSTITUTE OF POLYMER RESEARCH DRESDEN   (Germany)

^d CENTER FOR REGENERATIVE THERAPIES DRESDEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BONE; CYTOLOGY; PROTEINS; STEM CELLS;

BONE MARROW MICROENVIRONMENT; CONTINUOUS MONITORING; EXTRACELLULAR MATRICES; HEMATOPOIETIC STEM CELLS; HIGH THROUGHPUT SCREENING; MESENCHYMAL STROMAL CELLS; PROMOTER ACTIVITIES; TIME-DEPENDENT EFFECTS;

MONITORING;

ANGIOPOIETIN 1; COLLAGEN TYPE 1; MORPHOGEN; STROMAL CELL DERIVED FACTOR 1; VITRONECTIN; WNT3A PROTEIN; ANGPT1 PROTEIN, HUMAN; MESSENGER RNA; SCLEROPROTEIN;

ARTICLE; CELL ACTIVITY; CELL CULTURE MONITORING; CELL IMMORTALIZATION; CELL INTERACTION; CONCENTRATION RESPONSE; CONTROLLED STUDY; CULTURE OPTIMIZATION; EXTRACELLULAR MATRIX; FEEDER CELL; GENE CONSTRUCT; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; MESENCHYMAL STEM CELL; MESENCHYMAL STROMA CELL; MICROENVIRONMENT; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN MICROARRAY; REPORTER GENE; TRANSIENT EXPRESSION; CHEMISTRY; CYTOLOGY; GENETICS; METABOLISM; PROCEDURES; REPRODUCIBILITY; TISSUE SCAFFOLD;

ANGIOPOIETIN-1; CHEMOKINE CXCL12; EXTRACELLULAR MATRIX; EXTRACELLULAR MATRIX PROTEINS; GENES, REPORTER; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MESENCHYMAL STROMAL CELLS; REPRODUCIBILITY OF RESULTS; RNA, MESSENGER; TISSUE SCAFFOLDS;

EID: 84911909507     PISSN: 19373384     EISSN: 19373392     Source Type: Journal    
DOI: 10.1089/ten.tec.2013.0590     Document Type: Article

References (39)

1. Sacchetti, B., Funari, A., Michienzi, S., Di Cesare, S., Piersanti, S., Saggio, I., Tagliafico, E., Ferrari, S., Robey, P.G., Riminucci, M., and Bianco, P. Self-renewing osteoprogenitors in bone marrow sinusoids can organize a hematopoietic microenvironment. Cell 131, 324, 2007.
2. Mendez-Ferrer, S., Michurina, T.V., Ferraro, F., Mazloom, A.R., MacArthur, B.D., Lira, S.A., Scadden, D.T., Ma'ayan, A., Enikolopov, G.N., and Frenette, P.S. Mesenchymal and haematopoietic stem cells form a unique bone marrow niche. Nature 466, 829, 2010.
3. Sugiyama, T., Kohara, H., Noda, M., and Nagasawa, T. Maintenance of the hematopoietic stem cell pool by CXCL12-CXCR4 chemokine signaling in bone marrow stromal cell niches. Immunity 25, 977, 2006.
4. Sorrentino, A., Ferracin, M., Castelli, G., Biffoni, M., Tomaselli, G., Baiocchi, M., Fatica, A., Negrini, M., Peschle, C., and Valtieri, M. Isolation and characterization of CD146(+) multipotent mesenchymal stromal cells. Exp Hematol 36, 1035, 2008.
5. Barker, J.E. Sl/Sl(D) Hematopoietic progenitors are deficient in-situ. Exp Hematology 22, 174, 1994.
6. Yoshihara, H., Arai, F., Hosokawa, K., Hagiwara, T., Takubo, K., Nakamura, Y., Gomei, Y., Iwasaki, H., Matsuoka, S., Miyamoto, K., Miyazaki, H., Takahashi, T., and Suda, T. Thrombopoietin/MPL signaling regulates hematopoietic stem cell quiescence and interaction with the osteoblastic niche. Cell Stem Cell 1, 685, 2007.
7. Arai, F., Hirao, A., Ohmura, M., Sato, H., Matsuoka, S., Takubo, K., Ito, K., Koh, G.Y., and Suda, T. Tie2/angiopoietin-1 signaling regulates hematopoietic stem cell quiescence in the bone marrow niche. Cell 118, 149, 2004.
8. Calvi, L.M., Adams, G.B., Weibrecht, K.W., Weber, J.M., Olson, D.P., Knight, M.C., Martin, R.P., Schipani, E., Divieti, P., Bringhurst, F.R., Milner, L.A., Kronenberg, H.M., and Scadden, D.T. Osteoblastic cells regulate the haematopoietic stem cell niche. Nature 425, 841, 2003.
9. Ichii, M., Frank, M.B., Iozzo, R.V., and Kincade, P.W. The canonical Wnt pathway shapes niches supportive of hematopoietic stem/progenitor cells. Blood 119, 1683, 2012.
10. Ikushima, Y.M., Arai, F., Hosokawa, K., Toyama, H., Takubo, K., Furuyashiki, T., Narumiya, S., and Suda, T. Prostaglandin E2 regulates murine hematopoietic stem/progenitor cells directly via EP4 receptor and indirectly through mesenchymal progenitor cells. Blood 121, 1995, 2013.
11. Seiffert, D. Detection of vitronectin in mineralized bone matrix. J Histochem Cytochem 44, 275, 1996.
12. Nilsson, S.K., Debatis, M.E., Dooner, M.S., Madri, J.A., Quesenberry, P.J., and Becker, P.S. Immunofluorescence characterization of key extracellular matrix proteins in murine bone marrow in situ. J Histochem Cytochem 46, 371, 1998.
13. Nilsson, S.K., Johnston, H.M., Whitty, G.A., Williams, B., Webb, R.J., Denhardt, D.T., Bertoncello, I., Bendall, L.J., Simmons, P.J., and Haylock, D.N. Osteopontin, a key component of the hematopoietic stem cell niche and regulator of primitive hematopoietic progenitor cells. Blood 106, 1232, 2005.
14. Stier, S., Ko, Y., Forkert, F., Lutz, C., Neuhaus, T., Grunewald, E., Cheng, T., Dombkowski, D., Calvi, L.M., Rittling, S.R., and Scadden, D.T. Osteopontin is a hematopoietic stem cell niche component that negatively regulates stem cell pool size. J Exp Med 201, 1781, 2005.
15. Docheva, D., Popov, C., Mutschler, W., and Schieker, M. Human mesenchymal stem cells in contact with their environment: surface characteristics and the integrin system. J Cell Mol Med 11, 21, 2007.
16. Seib, F.P., Müller, K., Franke, M., Grimmer, M., Bornha üser, M., and Werner, C. Engineered extracellular matrices modulate the expression profile and feeder properties of bone marrow-derived human multipotent mesenchymal stromal cells. Tissue Eng Part A 15, 3161, 2009.
17. Lanfer, B., Seib, F.P., Freudenberg, U., Stamov, D., Bley, T., Bornhaüser, M., and Werner, C. The growth and differentiation of mesenchymal stem and progenitor cells cultured on aligned collagen matrices. Biomaterials 30, 5950, 2009.
18. Huang, N.F., Chu, J., Lee, R.J., and Li, S. Biophysical and chemical effects of fibrin on mesenchymal stromal cell gene expression. Acta Biomater 6, 3947, 2010.
19. Lai, Y.L., Sun, Y., Skinner, C.M., Son, E.L., Lu, Z.D., Tuan, R.S., Jilka, R.L., Ling, J.A., and Chen, X.D. Reconstitution of marrow-derived extracellular matrix ex vivo: a robust culture system for expanding large-scale highly functional human mesenchymal stem cells. Stem Cells Dev 19, 1095, 2010.
20. Pei, M., He, F., and Kish, V.L. Expansion on extracellular matrix deposited by human bone marrow stromal cells facilitates stem cell proliferation and tissue-specific lineage potential. Tissue Eng Part A 17, 3067, 2011.
21. Prewitz, M.C., Seib, F.P., von Bonin, M., Friedrichs, J., Stissel, A., Niehage, C., Mü ller, K, Anastassiadis, K., Waskow, C., Hoflack, B., Bornhaüser, M., and Werner, C. Tightly anchored tissue-mimetic matrices as instructive stem cell microenvironments. Nat Methods 10, 788, 2013.
22. Klein, G. The Extracellular-matrix of the hematopoietic microenvironment. Experientia 51, 914, 1995.
23. Lin, F.B., Ren, X.D., Pan, Z., Macri, L., Zong, W.X., Tonnesen, M.G., Rafailovich, M., Bar-Sagi, D., and Clark, R.A.F. Fibronectin growth factor-binding domains are required for fibroblast survival. J Invest Dermatol 131, 84, 2011.
24. Wijelath, E.S., Rahman, S., Namekata, M., Murray, J., Nishimura, T., Mostafavi-Pour, Z., Patel, Y., Suda, Y., Humphries, M.J., and Sobel, M. Heparin-II domain of fibronectin is a vascular endothelial growth factor-binding domain-enhancement of VEGF biological activity by a singular growth factor/matrix protein synergism. Circ Res 99, 853, 2006.
25. Rahman, S., Patel, Y., Murray, J., Patel, K.V., Sumathipala, R., Sobel, M., and Wijelath, E.S. Novel hepatocyte growth factor (HGF) binding domains on fibronectin and vitronectin coordinate a distinct and amplified Met-integrin induced signalling pathway in endothelial cells. BMC Cell Biol 6, 8, 2005.
26. Bocker, W., Yin, Z., Drosse, I., Haasters, F., Rossmann, O., Wierer, M., Popov, C., Locher, M., Mutschler, W., Docheva, D., and Schieker, M. Introducing a single-cellderived human mesenchymal stem cell line expressing hTERT after lentiviral gene transfer. J Cell Mol Med 12, 1347, 2008
27. Zufferey, R., Dull, T., Mandel, R.J., Bukovsky, A., Quiroz, D., Naldini, L., and Trono, D. Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery. J Virol 72, 9873, 1998.
28. Schambach, A., Bohne, J., Chandra, S., Will, E., Margison, G.P., Williams, D.A., and Baum, C. Equal potency of gammaretroviral and lentiviral SIN vectors for expression of O-6-methylguanine-DNA methyltransferase in hematopoietic cells. Mol Ther 13, 391, 2006.
29. Reya, T., O'Riordan, M., Okamura, R., Devaney, E., Willert, K., Nusse, R., and Grosschedl, R. Wnt signaling regulates B lymphocyte proliferation through a LEF-1 dependent mechanism. Immunity 13, 15, 2000.
30. Hosokawa, K., Arai, F., Yoshihara, H., Iwasaki, H., Hembree, M., Yin, T., Nakamura, Y., Gomei, Y., Takubo, K., Shiama, H., Matsuoka, S., Li, L., and Suda, T. Cadherinbased adhesion is a potential target for niche manipulation to protect hematopoietic stem cells in adult bone marrow. Cell Stem Cell 6, 194, 2010.
31. Dishowitz, M.I., Zhu, F., Sundararaghavan, H.G., Ifkovits, J.L., Burdick, J.A., and Hankenson, K.D. Jagged1 immobilization to an osteoconductive polymer activates the Notch signaling pathway and induces osteogenesis. J Biomed Mater Res A 2013 [Epub ahead of print]; DOI: 10.1002/jbm.a.34825.
32. Gobaa, S., Hoehnel, S., Roccio, M., Negro, A., Kobel, S., and Lutolf, M.P. Artificial niche microarrays for probing single stem cell fate in high throughput. Nat Methods 8, 949, 2011.
33. Peerani, R., and Zandstra, P.W. Enabling stem cell therapies through synthetic stem cell-niche engineering. J Clin Invest 120, 60, 2010.
34. Anderson, D.G., Levenberg, S., and Langer, R. Nanoliterscale synthesis of arrayed biomaterials and application to human embryonic stem cells. Nat Biotechnol 22, 863, 2004.
35. Flaim, C.J., Chien, S., and Bhatia, S.N. An extracellular matrix microarray for probing cellular differentiation. Nat Methods 2, 119, 2005.
36. Soen, Y., Mori, A., Palmer, T.D., and Brown, P.O. Exploring the regulation of human neural precursor cell differentiation using arrays of signaling microenvironments. Mol Syst Biol 2, 37, 2006.
37. Lutolf, M.P., Doyonnas, R., Havenstrite, K., Koleckar, K., and Blau, H.M. Perturbation of single hematopoietic stem cell fates in artificial niches. Integr Biol (Camb) 1, 59, 2009.
38. Berger, J., Hauber, J., Hauber, R., Geiger, R., and Cullen, B.R. Secreted placental alkaline-phosphatase-a powerful new quantitative indicator of gene-expression in eukaryotic cells. Gene 66, 1, 1988.
39. Tannous, B.A. Gaussia luciferase reporter assay for monitoring biological processes in culture and in vivo. Nat Protocols 4, 582, 2009.