Resveratrol supplementation does not augment performance adaptations or fibre-type–specific responses to high-intensity interval training in humans

Applied Physiology, Nutrition and Metabolism

Volumn 39, Issue 11, 2014, Pages 1305-1313

  Scribbans, Trisha D ^a   Ma, Jasmin K ^a   Edgett, Brittany A ^a   Vorobej, Kira A ^b   Mitchell, Andrew S ^b   Zelt, Jason G E ^a   Simpson, Craig A ^a   Quadrilatero, Joe ^b   Gurd, Brendon J ^a  

^a QUEEN S UNIVERSITY   (Canada)

^b UNIVERSITY OF WATERLOO   (Canada)

Author keywords

Anaerobic exercise capacity; Antioxidant; High intensity interval training (HIIT); Oxidative glycolytic capacity; PGC 1 ; Resveratrol; Skeletal muscle

Indexed keywords

OXYGEN; RESVERATROL; STILBENE DERIVATIVE;

ADAPTATION; BIOPSY; CONTROLLED STUDY; DOUBLE BLIND PROCEDURE; DRUG EFFECTS; EXERCISE; EXERCISE TEST; FEMALE; GENE EXPRESSION; HUMAN; MALE; METABOLISM; PHYSIOLOGY; RANDOMIZED CONTROLLED TRIAL; REAL TIME POLYMERASE CHAIN REACTION; SKELETAL MUSCLE; SKELETAL MUSCLE CELL; YOUNG ADULT;

ADAPTATION, PHYSIOLOGICAL; BIOPSY; DOUBLE-BLIND METHOD; EXERCISE; EXERCISE TEST; FEMALE; GENE EXPRESSION; HUMANS; MALE; MUSCLE FIBERS, SKELETAL; MUSCLE, SKELETAL; OXYGEN; REAL-TIME POLYMERASE CHAIN REACTION; STILBENES; YOUNG ADULT;

EID: 84910144768     PISSN: 17155312     EISSN: 17155320     Source Type: Journal    
DOI: 10.1139/apnm-2014-0070     Document Type: Article

References (46)

1. Baur, J.A., and Sinclair, D.A. 2006. Therapeutic potential of resveratrol: the in vivo evidence. Nat. Rev. Drug Discov. 5(6): 493-506. doi:10.1038/nrd2060. PMID:16732220.
2. Bergstrom, J. 1975. Percutaneous needle biopsy of skeletal muscle in physiological and clinical research. Scand. J. Clin. Lab. Invest. 35(7): 609-616. doi:10. 3109/00365517509095787. PMID:1108172.
3. Blanco, C.E., Sieck, G.C., and Edgerton, V.R. 1988. Quantitative histochemical determination of succinic dehydrogenase activity in skeletal muscle fibres. Histochem. J. 20(4): 230-243. doi:10.1007/BF01747468. PMID:3209423.
4. Bloemberg, D., and Quadrilatero, J. 2012. Rapid determination of myosin heavy chain expression in rat, mouse, and human skeletal muscle using multicolor immunofluorescence analysis. PLoS ONE. 7(4): e35273. doi:10.1371/journal. pone.0035273. PMID:22530000.
5. Brown, V.A., Patel, K.R., Viskaduraki, M., Crowell, J.A., Perloff, M., Booth, T.D., et al. 2010. Repeat dose study of the cancer chemopreventive agent resveratrol in healthy volunteers: safety, pharmacokinetics, and effect on the insulin-like growth factor axis. Cancer Res. 70(22): 9003-9011. doi:10.1158/ 0008-5472.CAN-10-2364. PMID:20935227.
6. Burgomaster, K.A., Howarth, K.R., Phillips, S.M., Rakobowchuk, M., MacDonald, M.J., McGee, S.L., et al. 2008. Similar metabolic adaptations during exercise after low volume sprint interval and traditional endurance training in humans. J. Physiol. 586(1): 151-160. PMID:17991697.
7. Canto, C., and Auwerx, J. 2009. PGC-1α, SIRT1 and AMPK, an energy sensing network that controls energy expenditure. Curr. Opin. Lipidol. 20(2): 98-105. doi:10.1097/MOL.0b013e328328d0a4. PMID:19276888.
8. Chomczynski, P., and Sacchi, N. 2006. The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twentysomething years on. Nat. Protoc. 1(2): 581-585. doi:10.1038/nprot.2006.83. PMID:17406285.
9. Cohen, J. 1988. Statistical Power Analysis for the Behavioral Sciences. Routledge, Hillsdale, N.J., USA.
10. Crandall, J.P., Oram, V., Trandafirescu, G., Reid, M., Kishore, P., Hawkins, M., et al. 2012. Pilot Study of Resveratrol in Older Adults With Impaired Glucose Tolerance. J. Gerontol. A Biol. Sci. Med. Sci. 67(12): 1307-1312. doi:10.1093/ gerona/glr235. PMID:22219517.
11. Dolinsky, V.W., Jones, K.E., Sidhu, R.S., Haykowsky, M., Czubryt, M.P., Gordon, T., et al. 2012. Improvements in skeletal muscle strength and cardiac function induced by resveratrol during exercise training contribute to enhanced exercise performance in rats. J. Physiol. 590(11): 2783-2799. doi:10. 1113/jphysiol.2012.230490. PMID:22473781.
12. Edgett, B.A., Foster, W.S., Hankinson, P.B., Simpson, C.A., Little, J.P., Graham, R.B., et al. 2013. Dissociation of Increases in PGC-1α and Its Regulators from Exercise Intensity and Muscle Activation Following Acute Exercise. PLoS ONE, 8(8): e71623. doi:10.1371/journal.pone.0071623. PMID:23951207.
13. Faul, F., Erdfelder, E., Lang, A.-G., and Buchner, A. 2007. G*Power 3: A flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behavior Research Methods, 39(2): 175-191. doi:10.3758/BF03193146. PMID:17695343.
14. Gliemann, L., Schmidt, J.F., Olesen, J., Bienso, R.S., Peronard, S.L., Grandjean, S.U., et al. 2013. Resveratrol blunts the positive effects of exercise training on cardiovascular health in aged men. J. Physiol. 591(20): 5047-5059. doi:10.1113/jphysiol.2013.258061. PMID:23878368.
15. Gomez-Cabrera, M.-C., Domenech, E., Romagnoli, M., Arduini, A., Borras, C., Pallardo, F.V., et al. 2008. Oral administration of vitamin C decreases muscle mitochondrial biogenesis and hampers training-induced adaptations in endurance performance. Am. J. Clin. Nutr. 87(1): 142-149. PMID:18175748.
16. Halkjaer-Kristensen, J.J., and Ingemann-Hansen, T.T. 1979. Microphotometric analysis of NADH-tetrazolium reductase and alpha-glycerophosphate dehydrogenase in human quadriceps muscle. Histochem. J. 11(2): 127-136. doi:10. 1007/BF01002990. PMID:155667.
17. Hart, N., Sarga, L., Csende, Z., Koltai, E., Koch, L.G., Britton, S.L., et al. 2013. Resveratrol enhances exercise training responses in rats selectively bred for high running performance. Food and Chemical Toxicology, 61: 53-59. doi: 10.1016/j.fct.2013.01.051. PMID:23422033.
18. Hawley, S.A., Ross, F.A., Chevtzoff, C., Green, K.A., Evans, A., Fogarty, S., et al. 2010. Use of cells expressing y subunit variants to identify diverse mechanisms of AMPK activation. Cell Metab. 11(6): 554-565. doi:10.1016/j.cmet.2010. 04.001. PMID:20519126.
19. Higashida, K., Kim, S.H., Jung, S.R., Asaka, M., Holloszy, J.O., and Han, D.-H. 2013. Effects of resveratrol and SIRT1 on PGC-1α activity and mitochondrial biogenesis: a reevaluation. Plos Biol. 11: e1001603. doi:10.1371/journal.pbio.1001603. PMID:23874150.
20. Joyner, M.J. 2013. Food for thought - resveratrol vs. exercise training. J. Physiol. 591(20): 4953-4953. doi:10.1113/jphysiol.2013.263004.
21. Koopman, R.R., Schaart, G.G., and Hesselink, M.K.M. 2001. Optimisation of oil red O staining permits combination with immunofluorescence and automated quantification of lipids. Histochem. Cell Biol. 116(1): 63-68. doi:10.1007/ s004180100297. PMID:11479724.
22. Lagouge, M., Argmann, C., Gerhart-Hines, Z., Meziane, H., Lerin, C., Daussin, F., et al. 2006. Resveratrol improves mitochondrial function and protects against metabolic disease by activating SIRT1 and PGC-1α. Cell, 127(6): 1109- 1122. doi:10.1016/j.cell.2006.11.013. PMID:17112576.
23. Larsen, S., Nielsen, J., Hansen, C.N., Nielsen, L.B., Wibrand, F., Stride, N., et al. 2012. Biomarkers of mitochondrial content in skeletal muscle of healthy young human subjects. J. Physiol. 590(14): 3349-3360. doi:10.1113/jphysiol. 2012.230185. PMID:22586215.
24. Livak, K.J., and Schmittgen, T.D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods, 25(4): 402-408. doi:10.1006/meth.2001.1262. PMID:11846609.
25. Ma, J.K., Scribbans, T.D., Edgett, B.A., Colin Boyd, J., Simpson, C.A., Little, J.P., et al. 2013. Extremely low-volume, high-intensity interval training improves exercise capacity and increases mitochondrial protein content in human skeletal muscle. Open J. Mol. Integ. Phys. 03(04): 202-210. doi:10.4236/ojmip. 2013.34027.
26. McRae, G., Payne, A., Zelt, J.G.E., Scribbans, T.D., Jung, M.E., Little, J.P., et al. 2012. Extremely low volume, whole-body aerobic-resistance training improves aerobic fitness and muscular endurance in females. Appl. Physiol. Nutr. Metab. 37: 1124-1131. doi:10.1139/h2012-093. PMID:22994393.
27. Menzies, K.J., Singh, K., Saleem, A., and Hood, D.A. 2013. Sirtuin 1-mediated Effects of Exercise and Resveratrol on Mitochondrial Biogenesis. J. Biol. Chem. 288(10): 6968-6979. doi:10.1074/jbc.M112.431155. PMID:23329826.
28. Murase, T., Haramizu, S., Ota, N., and Hase, T. 2008. Suppression of the agingassociated decline in physical performance by a combination of resveratrol intake and habitual exercise in senescence-accelerated mice. Biogerontology, 10(4): 423-434. doi:10.1007/s10522-008-9177-z. PMID:18830683.
29. Paulsen, G., Cumming, K.T., Holden, G., Hallen, J., Ronnestad, B.R., Sveen, O., et al. 2014. Vitamin C and E supplementation hampers cellular adaptation to endurance training in humans: a double-blind randomized controlled trial. J. Physiol. doi:10.1113/jphysiol.2013.267419.
30. Pearson, K.J., Baur, J.A., Lewis, K.N., Peshkin, L., Price, N.L., Labinskyy, N., et al. 2008. Resveratrol delays age-related deterioration and mimics transcriptional aspects of dietary restriction without extending life span. Cell Metab. 8(2): 157-168. doi:10.1016/j.cmet.2008.06.011. PMID:18599363.
31. Perry, C.G.R., Heigenhauser, G.J.F., Bonen, A., and Spriet, L.L. 2008. Highintensity aerobic interval training increases fat and carbohydrate metabolic capacities in human skeletal muscle. Appl. Physiol. Nutr. Metab. 33(6): 1112- 1123. doi:10.1139/H08-097. PMID:19088769.
32. Phillips, S.M., Green, H.J., Tarnopolsky, M.A., Heigenhauser, G.J., and Grant, S.M. 1996. Progressive effect of endurance training on metabolic adaptations in working skeletal muscle. Am. J. Physiol. 270(2 Pt. 1): E265-E272.
33. Poulsen, M.M., Vestergaard, P.F., Clasen, B.F., Radko, Y., Christensen, L.P., Stodkilde-Jorgensen, H., et al. 2013. High-dose resveratrol supplementation in obese men: an investigator-initiated, randomized, placebo-controlled clinical trial of substrate metabolism, insulin sensitivity, and body composition. Diabetes, 62(4): 1186-1195. doi:10.2337/db12-0975. PMID:23193181.
34. Quadrilatero, J., Bombardier, E., Norris, S.M., Talanian, J.L., Palmer, M.S., Logan, H.M., et al. 2010. Prolonged moderate-intensity aerobic exercise does not alter apoptotic signaling and DNA fragmentation in human skeletal muscle. Am. J. Physiol. Endocrinol. Metab. 298(3): E534-E547. doi:10.1152/ ajpendo.00678.2009. PMID:19996388.
35. Rasmussen, R. 2001. Quantification on the LightCycler. In Rapid Cycle Real-Time PCR. Edited by S. Meuer, C. Wittwer, and K.-I. Nakagawara. Springer. pp. 21-34. doi:10.1007/978-3-642-59524-0_3.
36. Shepherd, S.O., Cocks, M., Tipton, K.D., Ranasinghe, A.M., Barker, T.A., Burniston, J.G., et al. 2013. Sprint interval and traditional endurance training increase net intramuscular triglyceride breakdown and expression of perilipin 2 and 5. J. Physiol. 591(3): 657-675. doi:10.1113/jphysiol.2012.240952. PMID:23129790.
37. Skrobuk, P., Kraemer, S., Semenova, M.M., Zitting, A., and Koistinen, H.A. 2012. Acute exposure to resveratrol inhibits AMPK activity in human skeletal muscle cells. Diabetologia, 55(11): 3051-3060. doi:10.1007/s00125-012-2691-1. PMID: 22898769.
38. Smoliga, J.M., and Blanchard, O.L. 2013. Recent data do not provide evidence that resveratrol causes 'mainly negative' or 'adverse' effects on exercise training in humans. J. Physiol. 591(20): 5251-5252. doi:10.1113/jphysiol.2013.262956. PMID:24130323.
39. Tabata, I., Nishimura, K., Kouzaki, M., Hirai, Y., Ogita, F., Miyachi, M., et al. 1996. Effects of moderate-intensity endurance and high-intensity intermittent training on anaerobic capacity and VO2max. Med. Sci. Sports Exerc. 28(10): 1327-1330. doi:10.1097/00005768-199610000-00018. PMID:8897392.
40. Terada, S., Tabata, I., and Higuchi, M. 2004. Effect of high-intensity intermittent swimming training on fatty acid oxidation enzyme activity in rat skeletal muscle. Jpn. J. Physiol. 54(1): 47-52. doi:10.2170/jjphysiol.54.47. PMID:15040848.
41. Timmers, S., Konings, E., Bilet, L., Houtkooper, R.H., van de Weijer, T., Goossens, G.H., et al. 2011. Calorie restriction-like effects of 30 days of resveratrol supplementation on energy metabolism and metabolic profile in obese Humans. Cell Metab. 14(5): 612-622. doi:10.1016/j.cmet.2011.10.002. PMID:22055504.
42. Yeo, W.K., McGee, S.L., Carey, A.L., Paton, C.D., Garnham, A.P., Hargreaves, M., et al. 2010. Acute signalling responses to intense endurance training commenced with low or normal muscle glycogen. Exp. Physiol. 95(2): 351-358. doi:10.1113/expphysiol.2009.049353. PMID:19854796.
43. Yoshino, J., Conte, C., Fontana, L., Mittendorfer, B., Imai, S.-I., Schechtman, K.B., et al. 2012. Resveratrol Supplementation does not improve metabolic function in nonobese women with normal glucose tolerance. Cell Metab. 16(5): 658-664. doi:10.1016/j.cmet.2012.09.015. PMID:23102619.
44. Zang, M., Xu, S., Maitland-Toolan, K.A., Zuccollo, A., Hou, X., Jiang, B., et al. 2006. Polyphenols stimulate AMP-activated protein kinase, lower lipids, and inhibit accelerated atherosclerosis in diabetic LDL receptor-deficient mice. Diabetes, 55(8): 2180-2191. doi:10.2337/db05-1188. PMID:16873680.
45. Zheng, J., and Ramirez, V.D. 2000. Inhibition of mitochondrial proton F0F1- ATPase/ATP synthase by polyphenolic phytochemicals. Br. J. Pharmacol. 130(5): 1115-1123. doi:10.1038/sj.bjp.0703397. PMID:10882397.
46. Zini, R., Morin, C., Bertelli, A., Bertelli, A.A., and Tillement, J.P. 1999. Effects of resveratrol on the rat brain respiratory chain. Drugs Exp. Clin. Res. 25(2-3): 87-97. PMID:10370869.