Seamless stitching of biosynthetic gene cluster containing type I polyketide synthases using Red/ET mediated recombination for construction of stably co-existing plasmids

Gene

Volumn 554, Issue 2, 2015, Pages 233-240

  Su, Chun ^a   Zhao, Xin Qing ^a   Wang, Hai Na ^a   Qiu, Rong Guo ^a,b   Tang, Li ^a,b,c  

^a DALIAN UNIVERSITY OF TECHNOLOGY   (China)

^b Beijing Biostar Technologies Ltd   (China)

^c Beijing Biostar Technologies Ltd ^*  (China)

Author keywords

Co existing; Heterologous expression; Polyketide synthases (PKS); Precise seamless stitching; Red ET recombination

Indexed keywords

AMPICILLIN; APRAMYCIN; CHLORAMPHENICOL; DNA FRAGMENT; ENDONUCLEASE; FOSTRIECIN; KANAMYCIN; POLYKETIDE SYNTHASE; POLYKETIDE SYNTHASE TYPE I; TETRACYCLINE; UNCLASSIFIED DRUG; ESCHERICHIA COLI PROTEIN; RIBOSOME PROTEIN; RPSL PROTEIN, E COLI;

ARTICLE; BIOSYNTHESIS; CONTROLLED STUDY; DRUG SYNTHESIS; ESCHERICHIA COLI; GENE CASSETTE; GENE CLUSTER; GENE EXPRESSION; GENE SEQUENCE; GENETIC ENGINEERING; GENETIC RECOMBINATION; HETEROLOGOUS EXPRESSION; NONHUMAN; PLASMID; RECOMBINANT GENE; RESTRICTION FRAGMENT; DNA SEQUENCE; ENZYMOLOGY; GENE VECTOR; GENETICS; METABOLISM; MOLECULAR CLONING; MULTIGENE FAMILY; PROCEDURES;

CLONING, MOLECULAR; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC VECTORS; MULTIGENE FAMILY; PLASMIDS; POLYKETIDE SYNTHASES; RECOMBINATION, GENETIC; RIBOSOMAL PROTEINS; SEQUENCE ANALYSIS, DNA;

EID: 84910135412     PISSN: 03781119     EISSN: 18790038     Source Type: Journal    
DOI: 10.1016/j.gene.2014.10.022     Document Type: Article

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