General co-expression vectors for the overexpression of heterodimeric protein complexes in Escherichia coli

Protein Expression and Purification

Volumn 38, Issue 1, 2004, Pages 1-8

  Dzivenu, Oki K ^a   Park, Hyun Ho ^a   Wu, Hao ^a  

^a WEILL CORNELL MEDICAL COLLEGE   (United States)

Author keywords

Bacterial expression; Co expression; Plasmid vectors

Indexed keywords

BACTERIAL PROTEIN; HYBRID PROTEIN; MULTIPROTEIN COMPLEX;

ARTICLE; BACTERIOPHAGE T7; BIOLOGICAL MODEL; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENE VECTOR; GENETICS; METABOLISM; MOLECULAR CLONING; PLASMID; RESTRICTION MAPPING;

BACTERIAL PROTEINS; BACTERIOPHAGE T7; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC VECTORS; MODELS, BIOLOGICAL; MULTIPROTEIN COMPLEXES; PLASMIDS; RECOMBINANT FUSION PROTEINS; RESTRICTION MAPPING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; VECTORS;

EID: 5344225676     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.07.016     Document Type: Article

References (46)

1. P.T. Chan, H. Ohmori, J. Tomizawa, and J. Lebowitz Nucleotide sequence and gene organization of ColE1 DNA J. Biol. Chem. 260 1985 8925 8935
2. F.W. Larimer, R.J. Mural, and T.S. Soper Versatile protein engineering vectors for mutagenesis, expression and hybrid enzyme formation Protein Eng. 3 1990 227 231
3. J.S. Elce, C. Hegadorn, S. Gauthier, J.W. Vince, and P.L. Davies Recombinant calpain II: improved expression systems and production of a C105A active-site mutant for crystallography Protein Eng. 8 1995 843 848
4. K. Graham-Siegenthaler, S. Gauthier, P.L. Davies, and J.S. Elce Active recombinant rat calpain II. Bacterially produced large and small subunits associate both in vivo and in vitro J. Biol. Chem. 269 1994 30457 30460
5. W. Yang, L. Zhang, Z. Lu, W. Tao, and Z. Zhai A new method for protein coexpression in Escherichia coli using two incompatible plasmids Protein Expr. Purif. 22 2001 472 478
6. M. Better, C.P. Chang, R.R. Robinson, and A.H. Horwitz Escherichia coli secretion of an active chimeric antibody fragment Science 240 1988 1041 1043
7. C. Li, J.W. Schwabe, E. Banayo, and R.M. Evans Coexpression of nuclear receptor partners increases their solubility and biological activities Proc. Natl. Acad. Sci. USA 94 1997 2278 2283
8. M. Baedeker, and G.E. Schulz Overexpression of a designed 2.2 kb gene of eukaryotic phenylalanine ammonia-lyase in Escherichia coli FEBS Lett. 457 1999 57 60
9. A. Skerra, and A. Pluckthun Assembly of a functional immunoglobulin Fv fragment in Escherichia coli Science 240 1988 1038 1041
10. F.E. Chen, S. Kempiak, D.B. Huang, C. Phelps, and G. Ghosh Construction, expression, purification and functional analysis of recombinant NFkappaB p50/p65 heterodimer Protein Eng. 12 1999 423 428
11. J. Dong, and T.D. Porter Coexpression of mammalian cytochrome P450 and reductase in Escherichia coli Arch. Biochem. Biophys. 327 1996 254 259
12. S. Fribourg, C. Romier, S. Werten, Y.G. Gangloff, A. Poterszman, and D. Moras Dissecting the interaction network of multiprotein complexes by pairwise coexpression of subunits in E. coli J. Mol. Biol. 306 2001 363 373
13. W.L. Gerards, W.W. de Jong, H. Bloemendal, and W. Boelens The human proteasomal subunit HsC8 induces ring formation of other alpha-type subunits J. Mol. Biol. 275 1998 113 121
14. B.P. Halling, D.A. Yuhas, R.R. Eldridge, S.N. Gilbey, V.A. Deutsch, and J.D. Herron Expression and purification of the hormone binding domain of the Drosophila ecdysone and ultraspiracle receptors Protein Expr. Purif. 17 1999 373 386
15. L.A. Henricksen, C.B. Umbricht, and M.S. Wold Recombinant replication protein A: expression, complex formation, and functional characterization J. Biol. Chem. 269 1994 11121 11132
16. M. Ishiai, J.P. Sanchez, A.A. Amin, Y. Murakami, and J. Hurwitz Purification, gene cloning, and reconstitution of the heterotrimeric single-stranded DNA-binding protein from Schizosaccharomyces pombe J. Biol. Chem. 271 1996 20868 20878
17. P. Rucker, F.M. Torti, and S.V. Torti Recombinant ferritin: modulation of subunit stoichiometry in bacterial expression systems Protein Eng. 10 1997 967 973
18. S. Shuman Catalytic activity of vaccinia mRNA capping enzyme subunits coexpressed in Escherichia coli J. Biol. Chem. 265 1990 11960 11966
19. P. Widlak The DFF40/CAD endonuclease and its role in apoptosis Acta Biochim. Pol. 47 2000 1037 1044
20. C.L. Fisher, and G.K. Pei Modification of a PCR-based site-directed mutagenesis method Biotechniques 23 1997 570 571 574
21. A. Kuderova, E. Nanak, M. Truksa, and B. Brzobohaty Use of rifampicin in T7 RNA polymerase-driven expression of a plant enzyme: rifampicin improves yield and assembly Protein Expr. Purif. 16 1999 405 409
22. A.C. Chang, and S.N. Cohen Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid J. Bacteriol. 134 1978 1141 1156
23. Y. Nakano, Y. Yoshida, Y. Yamashita, and T. Koga Construction of a series of pACYC-derived plasmid vectors Gene 162 1995 157 158
24. N. Casali, A.P.D. Preston, in: Methods in Molecular Biology, vol. 235, Humana, Totowa, NJ, 2003, pp. xi, 316 p.: ill., 324 cm
25. O.K. Dzivenu, Structure-based mutational analysis of S. pombe p13Suc1, PhD thesis. University of Oxford Press (1999)
26. F.W. Studier, A.H. Rosenberg, J.J. Dunn, and J.W. Dubendorff Use of T7 RNA polymerase to direct expression of cloned genes Methods Enzymol. 185 1990 60 89
27. E. Sharif-Askari, A. Alam, E. Rheaume, P.J. Beresford, C. Scotto, K. Sharma, D. Lee, W.E. DeWolf, M.E. Nuttall, J. Lieberman, and R.P. Sekaly Direct cleavage of the human DNA fragmentation factor-45 by granzyme B induces caspase-activated DNase release and DNA fragmentation Embo J. 20 2001 3101 3113
28. H.M. Berman, J. Westbrook, Z. Feng, G. Gilliland, T.N. Bhat, H. Weissig, I.N. Shindyalov, and P.E. Bourne The protein data bank Nucleic Acids Res. 28 2000 235 242
29. Y. Bourne, M.H. Watson, M.J. Hickey, W. Holmes, W. Rocque, S.I. Reed, and J.A. Tainer Crystal structure and mutational analysis of the human CDK2 kinase complex with cell cycle-regulatory protein CksHs1 Cell 84 1996 863 874
30. P.D. Jeffrey, A.A. Russo, K. Polyak, E. Gibbs, J. Hurwitz, J. Massague, and N.P. Pavletich Mechanism of CDK activation revealed by the structure of a cyclinAâ€"CDK2 complex Nature 376 1995 313 320
31. C.E. Stebbins, W.G. Kaelin Jr., and N.P. Pavletich Structure of the VHL-ElonginCâ€"ElonginB complex: implications for VHL tumor suppressor function Science 284 1999 455 461
32. G. Wu, G. Xu, B.A. Schulman, P.D. Jeffrey, J.W. Harper, and N.P. Pavletich Structure of a beta- TrCP1â€"Skp1â€"beta-catenin complex: destruction motif binding and lysine specificity of the SCF(beta-TrCP1) ubiquitin ligase Mol. Cell 11 2003 1445 1456
33. K. Luger, A.W. Mader, R.K. Richmond, D.F. Sargent, and T.J. Richmond Crystal structure of the nucleosome core particle at 2.8 â., resolution Nature 389 1997 251 260
34. K. Luger, T.J. Rechsteiner, A.J. Flaus, M.M. Waye, and T.J. Richmond Characterization of nucleosome core particles containing histone proteins made in bacteria J. Mol. Biol. 272 1997 301 311
35. S. Tan, Y. Hunziker, D.F. Sargent, and T.J. Richmond Crystal structure of a yeast TFIIA/TBP/DNA complex Nature 381 1996 127 151
36. K. Zalenskaya, J. Lee, C.N. Gujuluva, Y.K. Shin, M. Slutsky, and A. Goldfarb Recombinant RNA polymerase: inducible overexpression, purification and assembly of Escherichia coli rpo gene products Gene 89 1990 7 12
37. S. Tan A modular polycistronic expression system for overexpressing protein complexes in Escherichia coli Protein Expr. Purif. 21 2001 224 234
38. P.A. Cole Chaperone-assisted protein expression Structure 4 1996 239 242
39. C. Vonrhein, U. Schmidt, G.A. Ziegler, S. Schweiger, I. Hanukoglu, and G.E. Schulz Chaperone-assisted expression of authentic bovine adrenodoxin reductase in Escherichia coli FEBS Lett. 443 1999 167 169
40. S.K. Burley, and J.B. Bonanno Structuring the universe of proteins Annu. Rev. Genomics Hum. Genet. 3 2002 243 262
41. A. Sali, R. Glaeser, T. Earnest, and W. Baumeister From words to literature in structural proteomics Nature 422 2003 216 225
42. K. Johnston, A. Clements, R.N. Venkataramani, R.C. Trievel, and R. Marmorstein Coexpression of proteins in bacteria using T7-based expression plasmids: expression of heteromeric cell-cycle and transcriptional regulatory complexes Protein Expr. Purif. 20 2000 435 443
43. N. Kholod, and T. Mustelin Novel vectors for co-expression of two proteins in E. coli Biotechniques 31 2001 322 323 326â€"328
44. G.J. Phillips, S.K. Park, and D. Huber High copy number plasmids compatible with commonly used cloning vectors Biotechniques 28 2000 400 402 404, 406
45. K. Skowronek, and A.A. Kasprzak A two-plasmid system for independent genetic manipulation of subunits of homodimeric proteins and selective isolation of chimeric dimers Anal. Biochem. 300 2002 185 191
46. S.C. Makrides Strategies for achieving high-level expression of genes in Escherichia coli Microbiol. Rev. 60 1996 512 538