Enhanced transcription rates in membrane-free protocells formed by coacervation of cell lysate

Proceedings of the National Academy of Sciences of the United States of America

Volumn 110, Issue 29, 2013, Pages 11692-11697

  Sokolova, Ekaterina ^a   Spruijt, Evan ^a   Hansen, Maike M K ^a   Dubuc, Emilien ^a   Groen, Joost ^a   Chokkalingam, Venkatachalam ^a   Piruska, Aigars ^a   Heus, Hans A ^a   Huck, Wilhelm T S ^a  

^a RADBOUD UNIVERSITY NIJMEGEN   (Netherlands)

Author keywords

Macromolecular crowding; Microdroplets

Indexed keywords

GREEN FLUORESCENT PROTEIN; MESSENGER RNA; RNA POLYMERASE;

ARTICLE; BINDING AFFINITY; CELL LYSATE; COACERVATION; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC TRANSCRIPTION; IN VIVO STUDY; NONHUMAN; PHASE SEPARATION; PHASE TRANSITION; PRIORITY JOURNAL; PROTEIN SYNTHESIS;

MACROMOLECULAR CROWDING; MICRODROPLETS;

ARTIFICIAL CELLS; ESCHERICHIA COLI; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; MACROMOLECULAR SUBSTANCES; MICROSCOPY, FLUORESCENCE; MODELS, BIOLOGICAL; PHASE TRANSITION; TRANSCRIPTION, GENETIC;

EID: 84880391309     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1222321110     Document Type: Article

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21. Rasmussen S, Chen LH, Nilsson M, Abe S (2003) Bridging nonliving and living matter. Artif Life 9(3):269-316.
22. Noireaux V, Libchaber A (2004) A vesicle bioreactor as a step toward an artificial cell assembly. Proc Natl Acad Sci USA 101(51)-17669-17674.
23. Szostak JW, Bartel DP, Luisi PL (2001) Synthesizing life. Nature 409(6818):387-390.
24. Hyman AA, Simons K (2012) Cell biology. Beyond oil and water - phase transitions in cells. Science 337(6098)-1047-1049.
25. Oparin AI (1953) The Origin of Life (Dover Publications, New York), 2nd Ed, p 270.
26. Fulton AB (1982) How crowded is the cytoplasm? Cell 30(2):345-347.
27. Daube SS, Bracha D, Buxboim A, Bar-Ziv RH (2010) Compartmentalization by directional gene expression. Proc Natl Acad Sci USA 107(7):2836-2841.
28. Strulson CA, Molden RC, Keating CD, Bevilacqua PC (2012) RNA catalysis through compartmentalization. Nat Chem 4(11):941-946.
29. Koga S, Williams DS, Perriman AW, Mann S (2011) Peptide-nucleotide microdroplets as a step towards a membrane-free protocell model. Nat Chem 3(9):720-724.
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34. Brangwynne CP, Mitchison TJ, Hyman AA (2011) Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes. Proc Natl Acad Sci USA 108(11):4334-4339.
35. Kaiser TE, Intine RV, DundrM (2008) De novo formation of a subnuclear body. Science 322(5908)-1713-1717.
36. Parker R, Sheth U (2007) P bodies and the control of mRNA translation and degradation. Mol Cell 25(5):635-646.
37. Albertsson PA (1958) Partition of proteins in liquid polymer-polymer two-phase systems. Nature 182(4637):709-711.
38. Baumgart T, et al. (2007) Large-scale fluid/fluid phase separation of proteins and lipids in giant plasma membrane vesicles. Proc Natl Acad Sci USA 104(9):3165-3170.